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    Coleopteran-toxic polypeptide compositions
    2.
    发明申请
    Coleopteran-toxic polypeptide compositions 失效
    鞘翅目毒性多肽组合物

    公开(公告)号：US20060253925A1

    公开(公告)日：2006-11-09

    申请号：US11485621

    申请日：2006-07-12

    申请人： Mark Rupar ,  William Donovan ,  Chih-Rei Chu ,  Elizabeth Pease ,  Yuping Tan ,  Annette Slaney ,  Thomas Malvar ,  James Baum

    发明人： Mark Rupar ,  William Donovan ,  Chih-Rei Chu ,  Elizabeth Pease ,  Yuping Tan ,  Annette Slaney ,  Thomas Malvar ,  James Baum

    IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N1/20 ,  C12N15/82 ,  C12N5/04 ,  C07K14/325

    CPC分类号： C07K14/325 ,  A01N63/02 ,  C12N15/8286 ,  Y02A40/162

    摘要： Disclosed are novel insecticidal polypeptides, and compositions comprising these polypeptides, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that contain nucleic acid segments that encode the disclosed δ-endotoxin polypeptides. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising these polynucleotide sequences, as well as methods for controlling an insect population, such as Colorado potato beetle, southern corn rootworm and western corn rootworm, and for conferring to a plant resistance to a target insect species.

    摘要翻译： 公开了新型杀虫多肽，以及包含这些多肽，其肽片段和特异性抗体的组合物。 还公开了载体，转化的宿主细胞和含有编码公开的δ-内毒素多肽的核酸片段的转基因植物。 还公开了鉴定相关多肽和多核苷酸的方法，制备和使用包含这些多核苷酸序列的转基因细胞的方法，以及用于控制昆虫种群的方法，例如科罗拉多马铃薯甲虫，南方玉米根虫和西方玉米根虫，并赋予 对植物对目标昆虫物种的抗性。

    Polypeptide compositions toxic to diabrotica insects, and methods of use
    5.
    发明申请
    Polypeptide compositions toxic to diabrotica insects, and methods of use 有权
    对双歧杆菌昆虫有毒的多肽组合物，以及使用方法

    公开(公告)号：US20060123512A1

    公开(公告)日：2006-06-08

    申请号：US11332654

    申请日：2006-01-13

    申请人： Anne-Marie Mettus ,  James Baum

    发明人： Anne-Marie Mettus ,  James Baum

    IPC分类号： A01H1/00 ,  C12N15/82 ,  C07K14/325 ,  C12N1/21 ,  C12N5/04

    CPC分类号： C07K14/325 ,  C07K2319/00 ,  C12N15/8286 ,  Y02A40/162 ,  Y10S530/825

    摘要： Disclosed is a novel Lepidopteran- and Coleopteran-active δ-endotoxin polypeptide, and compositions comprising the polypeptide, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that comprise nucleic acid segments encoding the polypeptide. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising the novel sequences of the invention, as well as methods for controlling an insect population, such as the Western Corn Rootworm and Colorado potato beetle, and for conferring to a plant population resistance to the target insect species.

    摘要翻译： 公开了一种新型鳞翅目昆虫和鞘翅目昆虫活性δ-内毒素多肽，以及包含多肽，其肽片段和特异性抗体的组合物。 还公开了载体，转化的宿主细胞和包含编码多肽的核酸片段的转基因植物。 还公开了鉴定相关多肽和多核苷酸的方法，制备和使用包含本发明新颖序列的转基因细胞的方法，以及用于控制昆虫种群的方法，例如西部玉米根虫和科罗拉多马铃薯甲虫，以及赋予 对植物群体抵抗目标昆虫物种。

    Transgenic plants expressing lepidopteran-active delta-endotoxins
    7.
    发明申请
    Transgenic plants expressing lepidopteran-active delta-endotoxins 有权
    表达鳞翅目昆虫活性δ-内毒素的转基因植物

    公开(公告)号：US20050155103A1

    公开(公告)日：2005-07-14

    申请号：US10999188

    申请日：2004-11-29

    申请人： James Baum ,  Amy Gilmer ,  Anne-Marie Mettus

    发明人： James Baum ,  Amy Gilmer ,  Anne-Marie Mettus

    IPC分类号： A01H5/00 ,  A01N63/00 ,  A01N63/02 ,  C07K14/325 ,  C07K16/12 ,  C12N1/21 ,  C12N15/09 ,  C12N15/31 ,  C12N15/82 ,  C12P21/02 ,  C12P21/08 ,  C12R1/38 ,  A01H1/00 ,  A01N65/00 ,  C12N5/04

    CPC分类号： C12R1/075 ,  C07K14/325 ,  C12N15/8286 ,  Y02A40/162

    摘要： Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding δ-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the α helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.

    摘要翻译： 公开了编码对鳞翅目昆虫具有杀虫活性的δ-内毒素的新型合成修饰的苏云金芽孢杆菌核酸片段。 还公开了由这些新型核酸序列编码的合成晶体蛋白质。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。 还公开了用于修饰，改变和诱变这些晶体蛋白（包括Cry1C）的结构域1中的α螺旋之间的特异性环区的方法，以产生遗传工程改造的重组cry *基因及其编码的具有改善的杀虫活性的蛋白质。 在优选的实施方案中，公开了新的Cry1C *氨基酸片段和编码它们的经修饰的cry1C *核酸序列。