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    Probe-type near-field confocal having feedback for adjusting probe distance
    8.
    发明授权
    Probe-type near-field confocal having feedback for adjusting probe distance 失效
    探头型近场共聚焦显微镜具有调整探头距离的反馈

    公开(公告)号：US5874726A

    公开(公告)日：1999-02-23

    申请号：US731093

    申请日：1996-10-09

    申请人： Philip G. Haydon

    发明人： Philip G. Haydon

    IPC分类号： G01Q60/18 ,  G02B21/00 ,  G01J1/20 ,  G01N21/64

    CPC分类号： G01Q60/22 ,  B82Y20/00 ,  B82Y35/00 ,  G01Q10/065 ,  G02B21/0072 ,  G02B21/0076 ,  Y10S977/853 ,  Y10S977/862

    摘要： A method and apparatus for achieving optical microscopic images and monitoring metabolic processes of living biological specimens such as cells at a resolution superior to the diffraction limit is disclosed. A primary difficulty in performing near-field scanning optical microscopy of living cells, e.g., determining the separation between the cell surface and the illuminating probe tip, is overcome by using a photon-density feedback method in which a fluorescent dye signal strength is monitored in the cell as the tip is brought to the cell surface, and registering a maximum value, at which point the tip begins to dimple the cell surface and can get no closer to the dye. Thereafter the tip is either maintained in contact with the membrane for point measurements of metabolic processes or is withdrawn a selected distance from the surface as measured against a corresponding decrease in the fluorescent dye signal strength The signal strength serves as a photon-density feedback for maintaining the probe tip at a constant elevation above the cell surface as scanning is performed or time-series measurements of metabolism are recorded. Advantageously, the apparatus also combines confocal means in the form of a pin-hole or the like for high-fidelity light detection in three dimensions from the cell surface in the near-field of the probe tip.

    摘要翻译： 公开了一种用于实现光学显微镜图像和监测活体生物样品例如细胞的分辨率高于衍射极限的分辨率的方法和装置。 通过使用其中监测荧光染料信号强度的光子密度反馈方法克服了执行活细胞的近场扫描光学显微镜（例如，确定细胞表面和照射探针尖端之间的分离）的主要困难， 将细胞作为尖端被带到细胞表面，并记录最大值，此时尖端开始使细胞表面凹陷并且不会更接近染料。 此后，尖端保持与膜接触，用于代谢过程的点测量，或者相对于荧光染料信号强度的相应降低测量，从表面取出选定距离。信号强度用作维持光子密度反馈 在进行扫描时进行细胞表面上方恒定高度的探针尖端或代谢的时间序列测量。 有利地，该装置还结合了针孔等形式的共焦装置，用于从探针尖端的近场中的细胞表面三维地进行高保真光检测。

    URIDINE NUCLEOSIDE DERIVATIVES, COMPOSITIONS AND METHODS OF USE
    9.
    发明申请
    URIDINE NUCLEOSIDE DERIVATIVES, COMPOSITIONS AND METHODS OF USE 审中-公开
    尿苷核苷衍生物，组合物和使用方法

    公开(公告)号：US20160075731A1

    公开(公告)日：2016-03-17

    申请号：US14775426

    申请日：2014-03-13

    申请人： Philip G. HAYDON ,  Jinbo LEE ,  TUFTS UNIVERSITY

    发明人： Philip G. Haydon ,  Jinbo Lee

    IPC分类号： C07H19/06

    摘要： This disclosure relates to uridine nucleoside derivatives, compositions comprising therapeutically effective amounts of those nucleoside derivatives and methods of using those nucleoside derivatives or compositions in treating disorders that are responsive to ligands, such as agonists, of P2Y6 receptor, e.g., neuronal disorders, including neurodegenerative disorders (e.g., Alzheimer's disease, Parkinson's disease) and traumatic CNS injury, pain, Down Syndrome (DS), glaucoma and inflammatory conditions.

    摘要翻译： 本公开涉及尿苷核苷衍生物，包含治疗有效量的那些核苷衍生物的组合物和使用这些核苷衍生物或组合物治疗对P2Y6受体的配体如激动剂例如神经元障碍（包括神经变性）起反应的疾病的方法 疾病（如阿尔茨海默病，帕金森病）和外伤性CNS损伤，疼痛，唐氏综合征（DS），青光眼和炎症状况。