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1.发明授权Method for rapid base sequencing in DNA and RNA with two base labeling 失效用于在两个碱基标记的DNA和RNA中进行快速碱基测序的方法
公开(公告)号：US5405747A
公开(公告)日：1995-04-11
申请号：US208506
申请日：1994-03-07
申请人： James H. Jett , Richard A. Keller , John C. Martin , Richard G. Posner , Babetta L. Marrone , Mark L. Hammond , Daniel J. Simpson
发明人： James H. Jett , Richard A. Keller , John C. Martin , Richard G. Posner , Babetta L. Marrone , Mark L. Hammond , Daniel J. Simpson
CPC分类号： C12Q1/6816 , C12Q1/6869 , Y10T436/143333
摘要： Method for rapid-base sequencing in DNA and RNA with two-base labeling and employing fluorescent detection of single molecules at two wavelengths. Bases modified to accept fluorescent labels are used to replicate a single DNA or RNA strand to be sequenced. The bases are then sequentially cleaved from the replicated strand, excited with a chosen spectrum of electromagnetic radiation, and the fluorescence from individual, tagged bases detected in the order of cleavage from the strand.
摘要翻译： 在DNA和RNA中进行快速碱基测序的方法，采用双碱基标记，并采用双波长单分子荧光检测。 用于接受荧光标记的碱基用于复制要测序的单个DNA或RNA链。 然后从复制的链中顺序地切割碱基，用所选择的电磁辐射谱激发，并且以从链断裂的顺序检测来自单个的标记碱基的荧光。
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公开(公告)号：US5558998A
公开(公告)日：1996-09-24
申请号：US486400
申请日：1995-06-05
IPC分类号： C12Q1/68 , G01N15/14 , G01N33/483 , G01N35/08
CPC分类号： C12Q1/6827 , G01N15/14 , Y10T436/117497
摘要： A method is provided for sizing DNA fragments using high speed detection systems, such as flow cytometry to determine unique characteristics of DNA pieces from a sample. In one characterization the DNA piece is fragmented at preselected sites to produce a plurality of DNA fragments. The DNA piece or the resulting DNA fragments are treated with a dye effective to stain stoichiometrically the DNA piece or the DNA fragments. The fluorescence from the dye in the stained fragments is then examined to generate an output functionally related to the number of nucleotides in each one of the DNA fragments. In one embodiment, the intensity of the fluorescence emissions from each fragment is linearly related to the fragment length. The distribution of DNA fragment sizes forms a characterization of the DNA piece for use in forensic and research applications.
摘要翻译： 提供了使用高速检测系统（例如流式细胞术）来定尺寸DNA片段的方法，以确定样品中DNA片段的独特特征。 在一个表征中，DNA片段在预选位点被片段化以产生多个DNA片段。 用有效染色DNA片段或DNA片段化学计量的染料处理DNA片段或得到的DNA片段。 然后检查染色片段中来自染料的荧光，以产生功能上与每个DNA片段中的核苷酸数相关的输出。 在一个实施方案中，来自每个片段的荧光发射强度与片段长度线性相关。 DNA片段大小的分布形成了用于法医学和研究应用的DNA片段的表征。
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公开(公告)号：US4962037A
公开(公告)日：1990-10-09
申请号：US105375
申请日：1987-10-07
申请人： James H. Jett , Richard A. Keller , John C. Martin , Robert K. Moyzis , Robert L. Ratliff , E. Brooks Shera , Carleton C. Stewart
发明人： James H. Jett , Richard A. Keller , John C. Martin , Robert K. Moyzis , Robert L. Ratliff , E. Brooks Shera , Carleton C. Stewart
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6813 , C12Q1/6823 , Y10S436/80 , Y10T436/143333 , Y10T436/25125
摘要： A method is provided for the rapid base sequencing of DNA or RNA fragments wherein a single fragment of DNA or RNA is provided with identifiable bases and suspended in a moving flow stream. An exonuclease sequentially cleaves individual bases from the end of the suspended fragment. The moving flow stream maintains the cleaved bases in an orderly train for subsequent detection and identification. In a particular embodiment, individual bases forming the DNA or RNA fragments are individually tagged with a characteristic fluorescent dye. The train of bases is then excited to fluorescence with an output spectrum characteristic of the individual bases. Accordingly, the base sequence of the original DNA or RNA fragment can be reconstructed.
摘要翻译： 提供了用于DNA或RNA片段的快速碱基测序的方法，其中DNA或RNA的单个片段具有可鉴定的碱基并悬浮在移动的流动中。 外切核酸酶从悬浮片段的末端依次切割各个碱基。 移动流动流将切割的基部保持在有序的列中，用于随后的检测和识别。 在一个具体实施方案中，形成DNA或RNA片段的单个碱基用特征荧光染料单独标记。 然后将基因列激发成具有各基因的输出光谱特征的荧光。 因此，可以重构原始DNA或RNA片段的碱基序列。
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4.发明授权
公开(公告)号：US6049380A
公开(公告)日：2000-04-11
申请号：US169025
申请日：1998-10-09
CPC分类号： G01N21/6428 , G01N21/6408
摘要： Single fluorescent molecules in a flowing sample stream are distinguished and identified using only a single laser excitation wavelength. A sample stream is formed containing a dilute mixture of single molecule fluorophores, wherein each one of the fluorophores is serially ordered in the sample stream. The sample stream is illuminated with s single excitation wavelength laser effective to excite each fluorophore one at a time. Fluorescence emission photons from each said fluorophore are detected. A burst size is determined for each fluorophore to identify each fluorophore. A pulsed laser may be used, where burst size and an intra-burst fluorescence decay rate for each fluorophore are determined simultaneously from the detected fluorescence emission photons. The burst size and the decay rate are correlated to identify each fluorophore.
摘要翻译： 流动样品流中的单个荧光分子仅使用单个激光激发波长来识别和识别。 形成包含单分子荧光团的稀混合物的样品流，其中每个荧光团在样品流中连续排列。 用单个激发波长激光器照射样品流，有效地一次激发每个荧光团。 检测来自每个所述荧光团的荧光发射光子。 确定每个荧光团的突发尺寸以识别每个荧光团。 可以使用脉冲激光，其中从检测到的荧光发射光子同时测定每个荧光团的突发尺寸和爆发内荧光衰减速率。 脉冲串大小和衰减速率相关，以识别每个荧光团。
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公开(公告)号：US06309886B1
公开(公告)日：2001-10-30
申请号：US09449280
申请日：1999-11-24
申请人： W. Patrick Ambrose , W. Kevin Grace , Peter M. Goodwin , James H. Jett , Alan Van Orden , Richard A. Keller
发明人： W. Patrick Ambrose , W. Kevin Grace , Peter M. Goodwin , James H. Jett , Alan Van Orden , Richard A. Keller
IPC分类号： G01N2164
CPC分类号： G01N21/6456 , G01N15/1434 , G01N15/1459 , G01N21/6458 , G01N2015/1006
摘要： Apparatus and method enable imaging multiple fluorescent sample particles in a single flow channel. A flow channel defines a flow direction for samples in a flow stream and has a viewing plane perpendicular to the flow direction. A laser beam is formed as a ribbon having a width effective to cover the viewing plane. Imaging optics are arranged to view the viewing plane to form an image of the fluorescent sample particles in the flow stream, and a camera records the image formed by the imaging optics.
摘要翻译： 装置和方法能够在单个流动通道中成像多个荧光样品颗粒。 流动通道限定流动流中的样品的流动方向，并且具有垂直于流动方向的观察平面。 激光束形成为具有有效覆盖观察平面的宽度的带状物。 成像光学器件被布置成观看观察平面以形成流动流中的荧光样品颗粒的图像，并且相机记录由成像光学元件形成的图像。
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公开(公告)号：US4402606A
公开(公告)日：1983-09-06
申请号：US238236
申请日：1981-02-25
CPC分类号： G01N27/628 , G01N21/3103
摘要： The disclosure relates to an optogalvanic intracavity detector and method for its use. Measurement is made of the amount of light absorbed by atoms, small molecules and ions in a laser cavity utilizing laser-produced changes in plasmas containing the same atoms, molecules, or ions.
摘要翻译： 本公开涉及一种光电腔内探测器及其使用方法。 使用激光产生的含有相同原子，分子或离子的等离子体变化的激光腔中的原子，小分子和离子吸收的光量进行测量。
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公开(公告)号：US08403615B1
公开(公告)日：2013-03-26
申请号：US12541932
申请日：2009-08-15
IPC分类号： B60P1/02
CPC分类号： B60P1/4457
摘要： A vehicle lift barrier is adapted to be mounted to vehicle lifts and the like that assist in loading and unloading cargo and provide a storage environment in which cargo is less likely to migrate from a cargo area into a passenger seating area or compartment. A barrier composed of a barricade is mounted to a vehicle lift, in some cases example by mounting the barrier to a generally vertical post, mast or the like or mounting the barrier to a lift platform, allowing the lift to operate normally while the barricade obstructs all or a substantial portion of the passage between the motor vehicle storage/cargo area and the passenger area. Where the barrier is mounted to a vertical post or the like on a vehicle lift, cut-out bracketing can be used to mate with the outer cross-sectional shape of the post to inhibit pivoting of the barrier about the post. Other bracket profiles can be used to enhance the barrier's protective advantages. A wrap-around sheet metal bracket configuration creates two flanges per bracket to reinforce bracing and to provide securing belt mounting points for a given vehicle lift. Such barriers can be used in motor vehicles with rear and/or side access-ways where vehicle lifts typically are provided and do not require special tie-downs or other cargo securing means, making such devices especially useful in vehicles that might serve a number of different users and/or that might transport a variety of types of cargo and/or mobility devices.
摘要翻译： 车辆升降障板适于安装到有助于装载和卸载货物的车辆升降机等，并提供货物不太可能从货物区域迁移到乘客座位区域或隔间中的存储环境。 由路障组成的障碍物安装在车辆升降机上，在某些情况下，例如通过将障碍物安装到大致垂直的立柱，桅杆等上，或将障碍物安装到升降平台上，允许升降机在路障阻碍时正常地操作 机动车辆存储/货物区域和乘客区域之间的通道的全部或大部分。 在障碍物安装在车辆升降机上的垂直立柱等上时，切割包围可用于与柱的外部横截面形状配合，以阻止围绕柱的枢转。 其他支架轮廓可用于增强屏障的保护优势。 环绕的金属板支架配置为每个支架创建两个法兰以加强支撑并为给定的车辆电梯提供固定带安装点。 这种障碍物可用于具有通常提供车辆升降机的后部和/或侧面通道的机动车辆，并且不需要特殊的绑扎或其他货物固定装置，使得这种装置在可能服务于多个 不同的用户和/或可能运输各种类型的货物和/或移动设备。
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8.发明授权
公开(公告)号：US5164891A
公开(公告)日：1992-11-17
申请号：US748240
申请日：1991-08-21
申请人： Richard A. Keller
发明人： Richard A. Keller
CPC分类号： G05F1/563 , H02M3/156 , Y02B70/1425
摘要： A voltage regulator generates an output voltage between an output line and an output reference voltage from an input voltage between an input line and an input reference voltage. The voltage regulator combines a self excited series resonant oscillator and a switching regulator control circuit. The self excited series resonant oscillator includes an oscillator circuit which produces an oscillating signal. The oscillating signal is rectified to produce a rectified signal. The rectified signal is filtered to produce the output voltage on the output line. The switching regulator control circuit switches the self excited series resonant oscillator on and off at a duty cycle which maintains the output voltage on the output line at a predetermined value.
摘要翻译： 电压调节器从输入线和输入参考电压之间的输入电压产生输出线和输出参考电压之间的输出电压。 电压调节器组合了自激式串联谐振振荡器和开关稳压器控制电路。 自激式串联谐振振荡器包括产生振荡信号的振荡电路。 振荡信号被整流以产生整流信号。 经整流的信号被滤波以在输出线上产生输出电压。 开关稳压器控制电路以保持输出线上的输出电压为预定值的占空比来开启和关闭自激式串联谐振振荡器。
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公开(公告)号：US4184127A
公开(公告)日：1980-01-15
申请号：US960193
申请日：1978-11-13
CPC分类号： G01N27/628 , G01N21/3103 , H01S3/137
摘要： An apparatus and method for utilizing the opto-galvanic effect to perform ectroscopic or analytic investigations of atomic or molecular species. A sample of the substance to be analyzed is vaporized in an analytical flame, gas discharge tube, high temperature furnace or the like, and the vapor is irradiated with chopped or pulsed variable wavelength monochromatic light. The electrical resistance of the vapor is monitored as the frequency of the radiation is tuned through one or more electronic transition frequencies of the substance. The resistance spectrum resembles the optical absorption spectrum of the species in the vapor. The optogalvanic effect may also be used to frequency lock a laser to a transition frequency of a substance in a gas discharge cell.
摘要翻译： 一种利用光电效应进行原子或分子物质的光谱或分析研究的装置和方法。 将待分析物质的样品在分析火焰，气体放电管，高温炉等中汽化，并用斩波或脉冲可变波长单色光照射蒸汽。 当通过物质的一个或多个电子跃迁频率调节辐射的频率时，监测蒸汽的电阻。 电阻谱类似于蒸汽中物质的光吸收光谱。 光电效应也可用于将激光器频率锁定到气体放电单元中的物质的过渡频率。
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公开(公告)号：US4771840A
公开(公告)日：1988-09-20
申请号：US38538
申请日：1987-04-15
申请人： Richard A. Keller
发明人： Richard A. Keller
CPC分类号： B62B5/0026 , B62B2207/00 , Y10S280/04
摘要： An articulated shopping cart-tractor unit with a seat is provided for the handicapped. The tractor unit is connected to a conventional shopping cart by a bracket and coupling.
摘要翻译： 为残疾人提供了带有座椅的铰接式购物车牵引车单元。 拖拉机单元通过支架和联轴器连接到传统的购物车。
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