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1.发明授权Modular nucleic acid-based circuits for counters, binary operations, memory and logic 有权用于计数器，二进制操作，存储器和逻辑的基于模块化的基于核酸的电路公开(公告)号：US08645115B2
公开(公告)日：2014-02-04
申请号：US13141165
申请日：2009-12-22
申请人： James J Collins , Timothy Lu
发明人： James J Collins , Timothy Lu
CPC分类号： C12Q1/6897 , C12N15/63 , C12N15/635 , C12Q2521/507
摘要： We have created novel engineered genetic counter designs and methods of use thereof that utilize DNA recombinases to provide modular systems, termed single invertase memory modules (SIMMs), for encoding memory in cells and cellular systems. Our designs are easily extended to compute to high numbers, by utilizing the >100 known recombinases to create subsequent modules. Flexibility in our engineered genetic counter designs is provided by daisy-chaining individual modular components, i.e., SIMMs together. These modular components of the engineered genetic counters can be combined in other network topologies to create circuits that perform, amongst other things, logic and memory. Our novel engineered genetic counter designs allow for the maintenance of memory and provide the ability to count between discrete states by expressing the recombinases between their cognate recognition sites.
摘要翻译： 我们已经创建了新颖的遗传算法设计及其使用方法，其利用DNA重组酶来提供称为单一转换酶存储器模块（SIMM）的模块化系统，用于对细胞和细胞系统中的存储器进行编码。 通过使用> 100个已知重组酶来创建后续模块，我们的设计可以轻松扩展到高数量计算。 我们设计的基因计数器设计中的灵活性是通过菊花链连接单个模块化组件（即SIMM）来提供的。 设计的遗传计数器的这些模块化组件可以组合在其他网络拓扑中，以创建执行逻辑和存储器的电路。 我们的新颖设计的基因计数器设计允许维持记忆，并提供通过在其同源识别位点之间表达重组酶来提供离散状态之间的计数能力。
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公开(公告)号：US09388425B2
公开(公告)日：2016-07-12
申请号：US12446300
申请日：2007-10-19
申请人： James J Collins , Tara L Deans
发明人： James J Collins , Tara L Deans
CPC分类号： C12N15/85 , C12N15/111 , C12N15/63 , C12N2310/14 , C12N2320/30 , C12N2800/30 , C12N2830/005 , C12N2830/006 , C12N2830/50 , C12N2840/102
摘要： The present invention relates generally the field of genetics, in particular methods, compositions and systems for controlling the inducible expression of transgenes, while eliminating background expression of transgene expression. The present invention relates to methods of use of the compositions and systems as disclosed herein for controlling the inducible expression of transgenes while eliminating background expression of transgene expression, such as use in, for example, the in generation of transgenic animals, use in therapeutic application and use in assays. In some embodiments, the present invention relates to a system of controlled expression of RNAi molecules which target binding sites in the untranslated regions of transgene, thereby the expression of the transgene is modulated and leakiness is reduced. The compositions and methods of the present invention can be used to for therapy, prophylaxis, research and diagnostics in diseases and disorders which afflict mammalian species, generation of transgenic animals, in the study of biological processes as well as for enhance performance of agricultural crops.
摘要翻译： 本发明一般涉及遗传学领域，特别是用于控制转基因诱导表达的方法，组合物和系统，同时消除转基因表达的背景表达。 本发明涉及如本文所公开的用于控制转基因诱导表达同时消除转基因表达的背景表达的用途的方法，例如在例如转基因动物的产生中的用途，在治疗应用中的用途 并用于测定。 在一些实施方案中，本发明涉及靶向转基因非翻译区中的结合位点的RNAi分子的受控表达系统，由此调节转基因的表达并降低渗透性。 本发明的组合物和方法可用于治疗，预防，研究和诊断在影响哺乳动物物种的疾病和病症中，产生转基因动物，研究生物过程以及增强农作物的性能。
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3.发明授权Engineered bacteriophages as adjuvants for antimicrobial agents and compositions and methods of use thereof 有权工程化噬菌体作为抗微生物剂的佐剂及其组合物及其使用方法公开(公告)号：US09056899B2
公开(公告)日：2015-06-16
申请号：US12812212
申请日：2009-01-12
IPC分类号： A01N63/00 , C12N7/01 , C07K14/005 , A61K35/76 , C12N15/113
CPC分类号： C07K14/005 , A61K35/76 , C12N15/113 , C12N2320/32 , C12N2330/30 , C12N2795/14122 , C12N2795/14132
摘要： The present invention relates to the treatment and prevention of bacteria and bacterial infections. In particular, the present invention relates to engineered bacteriophages used in combination with antimicrobial agents to potentiate the antimicrobial effect and bacterial killing by the antimicrobial agent. The present invention generally relates to methods and compositions comprising engineered bacteriophages and antimicrobial agents for the treatment of bacteria, and more particularly to bacteriophages comprising agents that inhibit antibiotic resistance genes and/or cell survival genes, and/or bacteriophages comprising repressors of SOS response genes or inhibitors of antimicrobial defense genes and/or expressing an agent which increases the sensitivity of bacteria to an antimicrobial agent in combination with at least one antimicrobial agent, and their use thereof.
摘要翻译： 本发明涉及细菌和细菌感染的治疗和预防。 特别地，本发明涉及与抗微生物剂组合使用的工程化噬菌体，以增强抗微生物效果和抗微生物剂的细菌杀伤作用。 本发明一般涉及包含工程化噬菌体和用于治疗细菌的抗微生物剂的方法和组合物，更具体地涉及包含抑制抗生素抗性基因和/或细胞存活基因的试剂和/或包含SOS应答基因阻遏物的噬菌体的噬菌体 或抗微生物防御基因的抑制剂和/或表达增加细菌对抗微生物剂与至少一种抗微生物剂的敏感性的试剂及其用途。
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4.发明授权公开(公告)号：US08182804B1
公开(公告)日：2012-05-22
申请号：US11662551
申请日：2005-09-12
申请人： James J Collins , Hideki Kobayashi , Mads Kearn , Michihiro Araki , Ari Friedland , Timothy Kuan-Ta Lu
发明人： James J Collins , Hideki Kobayashi , Mads Kearn , Michihiro Araki , Ari Friedland , Timothy Kuan-Ta Lu
CPC分类号： C12N9/2437 , A61L2/16 , C07K14/005 , C07K2319/00 , C12N7/00 , C12N2795/10222 , C12N2795/10243 , C12Y302/01004
摘要： The present invention provides engineered bacteriophages that express at least one biofilm degrading enzyme on their surface and uses thereof for degrading bacterial biofilms. The invention also provides genetically engineered bacteriophages expressing the biofilm degrading enzymes and proteins necessary for the phage to replicate in different naturally occurring biofilm producing bacteria. The phages of the invention allow a method of biofilm degradation by the use of one or only a few administration of the phage because the system using these phages is self perpetuating, and capable of degrading biofilm even when the concentration of bacteria within the biofilm is low.
摘要翻译： 本发明提供了在其表面上表达至少一种生物膜降解酶的工程化噬菌体及其用于降解细菌生物膜的工程噬菌体。 本发明还提供表达生物膜降解酶和蛋白质在不同天然存在的生物膜产生细菌中复制的噬菌体所需的遗传工程化噬菌体。 本发明的噬菌体允许通过使用一种或仅少量的噬菌体施用来生物膜降解的方法，因为使用这些噬菌体的系统是自我延续的，并且即使当生物膜内的细菌浓度低时也能降解生物膜 。
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5.发明授权Engineered enzymatically active bacteriophage and methods for dispersing biofilms 有权工程化的酶活性噬菌体和分散生物膜的方法公开(公告)号：US08153119B2
公开(公告)日：2012-04-10
申请号：US12337677
申请日：2008-12-18
CPC分类号： A01N63/02 , C12N7/00 , C12N9/2402 , C12N2795/10211 , C12N2795/10221 , C12N2795/10232 , C12Y207/07006 , C12Y302/01052
摘要： The present invention is directed to engineered enzymatically active bacteriophages that are both capable of killing the bacteria by lysis and dispersing the bacterial biofilm because they have been also engineered to express biofilm-degrading enzymes, particularly dispersin B (DspB), an enzyme that hydrolyzes β-1,6-N-acetyl-D-glucosamine, a crucial adhesion molecule needed for biofilm formation and integrity in Staphylococcus and E. coli, including E. coli K-12, as well as clinical isolates.
摘要翻译： 本发明涉及工程化的酶活性噬菌体，其能够通过裂解和分散细菌生物膜来杀死细菌，因为它们也被工程化以表达生物降膜酶，特别是分解B（DspB），其是水解＆bgr的酶 ; -1,6-N-乙酰基-D-葡糖胺，生物膜形成所需的关键粘附分子和葡萄球菌和大肠杆菌（包括大肠杆菌K-12）以及临床分离物中的完整性。
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6.发明申请ENGINEERED BACTERIOPHAGES AS ADJUVANTS FOR ANTIMICROBIAL AGENTS AND COMPOSITIONS AND METHODS OF USE THEREOF 有权工程化杀菌剂作为抗菌药物及其组合物的使用方法及其使用方法公开(公告)号：US20100322903A1
公开(公告)日：2010-12-23
申请号：US12812212
申请日：2009-01-12
CPC分类号： C07K14/005 , A61K35/76 , C12N15/113 , C12N2320/32 , C12N2330/30 , C12N2795/14122 , C12N2795/14132
摘要： The present invention relates to the treatment and prevention of bacteria and bacterial infections. In particular, the present invention relates to engineered bacteriophages used in combination with antimicrobial agents to potentiate the antimicrobial effect and bacterial killing by the antimicrobial agent. The present invention generally relates to methods and compositions comprising engineered bacteriophages and antimicrobial agents for the treatment of bacteria, and more particularly to bacteriophages comprising agents that inhibit antibiotic resistance genes and/or cell survival genes, and/or bacteriophages comprising repressors of SOS response genes or inhibitors of antimicrobial defense genes and/or expressing an agent which increases the sensitivity of bacteria to an antimicrobial agent in combination with at least one antimicrobial agent, and their use thereof.
摘要翻译： 本发明涉及细菌和细菌感染的治疗和预防。 特别地，本发明涉及与抗微生物剂组合使用的工程化噬菌体，以增强抗微生物效果和抗微生物剂的细菌杀伤作用。 本发明一般涉及包含工程化噬菌体和用于治疗细菌的抗微生物剂的方法和组合物，更具体地涉及包含抑制抗生素抗性基因和/或细胞存活基因的试剂和/或包含SOS应答基因阻遏物的噬菌体的噬菌体 或抗微生物防御基因的抑制剂和/或表达增加细菌对抗微生物剂与至少一种抗微生物剂的敏感性的试剂及其用途。
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