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1.发明授权Compounds and methods for assaying redox state of metabolically active cells and methods for measuring NAD(P)/NAD(P)H 有权用于测定代谢活性细胞的氧化还原状态的化合物和方法以及用于测量NAD（P）/ NAD（P）H的方法公开(公告)号：US09273343B2
公开(公告)日：2016-03-01
申请号：US13600579
申请日：2012-08-31
申请人： James J. Cali , Sarah Duellman , Dieter Klaubert , Donna Leippe , Martha O'Brien , John Shultz , Jolanta Vidugiriene , Wenhui Zhou , Mary Sobol
发明人： James J. Cali , Sarah Duellman , Dieter Klaubert , Donna Leippe , Martha O'Brien , John Shultz , Jolanta Vidugiriene , Wenhui Zhou , Mary Sobol
IPC分类号： C07D417/04 , C07D277/68 , C07D487/04 , C12Q1/32 , C07D493/10 , C12Q1/66 , C07D497/10 , C07F9/6558 , C07F9/6561 , G01N21/76 , G01N33/50 , G01N33/573 , G01N33/58
CPC分类号： C12Q1/32 , C07D277/68 , C07D417/04 , C07D487/04 , C07D493/10 , C07D497/10 , C07F9/65583 , C07F9/6561 , C12Q1/66 , G01N21/763 , G01N33/5038 , G01N33/5735 , G01N33/582
摘要： The present invention provides compounds and methods for assaying redox state of metabolically active cells and methods for assaying enzyme activity and/or metabolite level by coupling to redox defining co-factor NAD(P)/NAD(P)H measurement.
摘要翻译： 本发明提供了用于测定代谢活性细胞的氧化还原状态的化合物和方法，以及通过与氧化还原定义的辅因子NAD（P）/ NAD（P）H测量偶联来测定酶活性和/或代谢物水平的方法。
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公开(公告)号：US06893834B2
公开(公告)日：2005-05-17
申请号：US10693214
申请日：2003-10-24
IPC分类号： G01N33/53 , C12Q1/42 , C12Q1/48 , G01N33/543 , G01N33/573
摘要： Disclosed is a method and corresponding kit for assaying the presence, activity, or both, of an enzyme classified within an enzyme classification selected from the group consisting of EC 2.7.1, EC 3.1.3, and EC 3.1.4. The method generally includes the steps of reacting an enzyme with a substrate for a time sufficient to yield phosphorylated or dephosphorylated product; contacting the product with a binding matrix, whereby product is adhered to the matrix; and then analyzing the matrix for presence of, amount of, or both the presence and the amount of the product fixed to the matrix, whereby the presence, the activity, or both the presence and activity of the enzyme can be determined.
摘要翻译： 公开了用于测定分类在选自EC 2.7.1，EC 3.1.3和EC 3.1.4的酶分类内的酶的存在，活性或两者的方法和相应试剂盒。 该方法通常包括使酶与底物反应足以产生磷酸化或脱磷酸化产物的步骤; 使产物与结合基质接触，由此产物粘附到基质上; 然后分析该基质是否存在固定在基质上的产物的存在和量的存在和数量，从而可以确定酶的存在，活性或存在和活性。
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公开(公告)号：US10000792B2
公开(公告)日：2018-06-19
申请号：US12710087
申请日：2010-02-22
CPC分类号： C12Q1/485 , C12Q1/008 , C12Q1/44 , C12Q1/527 , C12Q1/66 , G01N33/74 , G01N2333/16 , G01N2333/726 , G01N2333/916 , G01N2333/988
摘要： The present invention relates in general to cellular analysis tools and more particularly to methods for detecting or determining cyclic nucleotide concentrations in samples. Samples containing cyclic nucleotides may be contacted with a cyclic nucleotide-dependent protein kinase and a detection system which includes a substrate for the cyclic nucleotide-dependent protein kinase. The activities in cyclic nucleotide related pathways may be measured using the detection system.
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公开(公告)号：US20110129821A1
公开(公告)日：2011-06-02
申请号：US12710087
申请日：2010-02-22
IPC分类号： C12Q1/68
CPC分类号： C12Q1/485 , C12Q1/008 , C12Q1/44 , C12Q1/527 , C12Q1/66 , G01N33/74 , G01N2333/16 , G01N2333/726 , G01N2333/916 , G01N2333/988
摘要： The present invention relates in general to cellular analysis tools and more particularly to methods for detecting or determining cyclic nucleotide concentrations in samples. Samples containing cyclic nucleotides may be contacted with a cyclic nucleotide-dependent protein kinase and a detection system which includes a substrate for the cyclic nucleotide-dependent protein kinase. The activities in cyclic nucleotide related pathways may be measured using the detection system.
摘要翻译： 本发明一般涉及细胞分析工具，更具体地涉及用于检测或确定样品中环核苷酸浓度的方法。 含有环状核苷酸的样品可以与环状核苷酸依赖性蛋白激酶和包含环状核苷酸依赖性蛋白激酶底物的检测系统接触。 可以使用检测系统测量环核苷酸相关途径中的活性。
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公开(公告)号：US20070172896A1
公开(公告)日：2007-07-26
申请号：US11634756
申请日：2006-12-06
CPC分类号： C12Q1/485 , C12Q1/008 , C12Q1/44 , C12Q1/527 , C12Q1/66 , G01N33/74 , G01N2333/16 , G01N2333/726 , G01N2333/916 , G01N2333/988
摘要： The present invention relates in general to cellular analysis tools and more particularly to methods for detecting or determining cyclic nucleotide concentrations in samples.
摘要翻译： 本发明一般涉及细胞分析工具，更具体地涉及用于检测或确定样品中环核苷酸浓度的方法。
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公开(公告)号：US06720162B2
公开(公告)日：2004-04-13
申请号：US09871424
申请日：2001-05-31
IPC分类号： C12Q142
摘要： Disclosed is a method and corresponding kit for assaying the presence, activity, or both, of an enzyme classified within an enzyme classification selected from the group consisting of EC 2.7.1, EC 3.1.3, and EC 3.1.4. The method generally includes the steps of reacting an enzyme with a substrate for a time sufficient to yield phosphorylated or dephosphorylated product; contacting the product with a binding matrix, whereby product is adhered to the matrix; and then analyzing the matrix for presence of, amount of, or both the presence and the amount of the product fixed to the matrix, whereby the presence, the activity, or both the presence and activity of the enzyme can be determined.
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