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公开(公告)号：US07811754B2
公开(公告)日：2010-10-12
申请号：US10941289
申请日：2004-09-09
申请人： James N. Herron , Samuel Tolley , Hsu-Kun Wang
发明人： James N. Herron , Samuel Tolley , Hsu-Kun Wang
CPC分类号： C12Q1/6827 , C12Q1/6874 , C12Q2565/501 , C12Q2563/107 , C12Q2561/113
摘要： Methods and apparatus for detecting single nucleotide polymorphisms in genes of interest are disclosed. A plurality of probes is immobilized on a planar waveguide. The probes comprise sequences complementary to a wildtype sequence of the gene of interest and complementary to a sequence of a known SNP in the gene of interest. A fluorescently-labeled analyte is flowed over the planar waveguide. The binding between the labeled analyte and each of the probes causes a change in the fluorescence signal. The SNP is detected by comparing the hybridization kinetics of the analyte with each of the probes. A method of detecting single nucleotide polymorphisms in a gene of interest by sequencing by hybridization is also disclosed.
摘要翻译： 公开了用于检测感兴趣的基因中单核苷酸多态性的方法和装置。 多个探针固定在平面波导上。 探针包含与感兴趣的基因的野生型序列互补的序列，并与感兴趣的基因中已知SNP的序列互补。 荧光标记的分析物在平面波导上流动。 标记的分析物与每个探针之间的结合导致荧光信号的变化。 通过比较分析物与每个探针的杂交动力学来检测SNP。 还公开了通过杂交测序检测目的基因中的单核苷酸多态性的方法。
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公开(公告)号：US20060228713A1
公开(公告)日：2006-10-12
申请号：US10941289
申请日：2004-09-09
申请人： James Herron , Samuel Tolley , Hsu-Kun Wang
发明人： James Herron , Samuel Tolley , Hsu-Kun Wang
CPC分类号： C12Q1/6827 , C12Q1/6874 , C12Q2565/501 , C12Q2563/107 , C12Q2561/113
摘要： Methods and apparatus for detecting single nucleotide polymorphisms in genes of interest are disclosed. A plurality of probes is immobilized on a planar waveguide. The probes comprise sequences complementary to a wildtype sequence of the gene of interest and complementary to a sequence of a known SNP in the gene of interest. A fluorescently-labeled analyte is flowed over the planar waveguide. The binding between the labeled analyte and each of the probes causes a change in the fluorescence signal. The SNP is detected by comparing the hybridization kinetics of the analyte with each of the probes. A method of detecting single nucleotide polymorphisms in a gene of interest by sequencing by hybridization is also disclosed.
摘要翻译： 公开了用于检测感兴趣的基因中单核苷酸多态性的方法和装置。 多个探针固定在平面波导上。 探针包含与感兴趣的基因的野生型序列互补的序列，并与感兴趣的基因中已知SNP的序列互补。 荧光标记的分析物在平面波导上流动。 标记的分析物与每个探针之间的结合导致荧光信号的变化。 通过比较分析物与每个探针的杂交动力学来检测SNP。 还公开了通过杂交测序检测目的基因中的单核苷酸多态性的方法。
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公开(公告)号：US20050153320A1
公开(公告)日：2005-07-14
申请号：US10984629
申请日：2004-11-08
申请人： James Herron , Samuel Tolley
发明人： James Herron , Samuel Tolley
CPC分类号： C12Q1/6825 , C12Q1/683 , C12Q2565/607 , C12Q2565/537 , C12Q2535/125
摘要： Genetic polymorphisms can change protein structure and function, altering dispositions to diseases and conditions. A single nucleotide polymorphism is the smallest genetic mutation and the most difficult to detect. However, single nucleotide polymorphisms also make up 90% of known genetic mutations, thus identifying such polymorphisms is essential. Single base extension uses the affinity of one base for its complementary base to detect polymorphisms, including single nucleotide polymorphisms. Planar waveguides are used as the platform for single base extension enabling rapid, real time detection of genetic polymorphisms. Detection limits in the picomolar range can be obtained. Signals from the non-matched DNA bases are in the range of the blank signal. Detection times of 5 minutes are reported.
摘要翻译： 遗传多态性可以改变蛋白质的结构和功能，改变疾病和状况。 单核苷酸多态性是最小的遗传突变，最难检测。 然而，单核苷酸多态性也占已知遗传突变的90％，因此识别这种多态性是至关重要的。 单碱基延伸使用一个碱基的互补碱基的亲和力来检测多态性，包括单核苷酸多态性。 平面波导用作单碱基扩增的平台，可以快速，实时地检测遗传多态性。 可以获得皮摩尔范围内的检测限。 来自非匹配DNA碱基的信号在空白信号的范围内。 报告检测时间为5分钟。
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公开(公告)号：US20050029445A1
公开(公告)日：2005-02-10
申请号：US10873752
申请日：2004-06-21
申请人： Edgar Lee , Alan Rockwood , Milton Lee , Samuel Tolley
发明人： Edgar Lee , Alan Rockwood , Milton Lee , Samuel Tolley
CPC分类号： H01J49/4205 , G01N27/624 , H01J49/0031 , H01J49/0081
摘要： A single set of electrodes, wherein different electrical potentials are applied to the single set of electrodes at different times in order to perform both ion mobility-based spectrometry and mass spectrometry (MS) on a sample of ions, wherein the ions are processed by performing ion mobility-based spectrometry and mass spectrometry in any sequence, any number of times, and as isolated or superposed procedures in order to trap, separate, fragment, and/or analyze charged particles and charged particles derived from atoms, molecules, particles, sub-atomic particles and ions.
摘要翻译： 单组电极，其中不同电位在不同时间施加到单组电极，以便对离子样品进行离子迁移率谱分析和质谱分析（MS），其中离子通过执行 基于离子迁移率的光谱和质谱法以任何顺序，任意次数，以及作为分离或重叠的方法，以捕获，分离，分离和/或分析带电粒子和衍生自原子，分子，粒子，子 原子粒子和离子。
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