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公开(公告)号：US08372605B2
公开(公告)日：2013-02-12
申请号：US13072314
申请日：2011-03-25
申请人： James Nadeau , Tobin J. Hellyer , Dolores M. Berger , William Nussbaumer , Robert Rosenstein , Andrew Kuhn , Sha-Sha Wang , Keith Edward Thornton
发明人： James Nadeau , Tobin J. Hellyer , Dolores M. Berger , William Nussbaumer , Robert Rosenstein , Andrew Kuhn , Sha-Sha Wang , Keith Edward Thornton
CPC分类号： C12Q1/6804 , C12Q1/6851
摘要： A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte.
摘要翻译： 提供了高灵敏度，低背景的免疫扩增测定法，其提供流线型工作流程，适用于临床相关样品如血液和其他体液的高通量测定。 该测定包括使用两个邻近成员，每个邻近成员包含与寡核苷酸缀合的分析物特异性结合成分。 结合分析物使邻近成员的寡核苷酸部分充分紧密接触，使寡核苷酸形成扩增子。 然后通过扩增扩增子和扩增核酸的检测来检测分析物的存在。 通过防止与分析物不复合的邻近成员形成假的或非特异性的扩增子来提高本发明的测定的灵敏度。
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公开(公告)号：US08323929B2
公开(公告)日：2012-12-04
申请号：US12419737
申请日：2009-04-07
CPC分类号： C12Q1/6827 , C12Q2565/1025 , C12Q2535/125 , C12Q2531/119 , C12Q2531/101
摘要： The invention employs an unlabeled signal primer comprising a 5′ adapter sequence for detection of variations in nucleic acid target sequences. The detection system further comprises a reporter probe, the 3′ end of which hybridizes to the complement of the 5′ adapter sequence of the signal primer to produce a 5′ overhang. Polymerase is used to fill in the overhang and synthesize the complement of the 5′ overhang of the reporter probe. Synthesis of the reporter probe complement is detected, either directly or indirectly, as an indication of the presence of the target.
摘要翻译： 本发明采用包含5'衔接子序列的未标记信号引物来检测核酸靶序列的变异。 检测系统还包括报道探针，其3'末端与信号引物的5'衔接子序列的互补体杂交以产生5'突出端。 聚合酶用于填充突出端并合成报告基因探针的5'突出端的互补体。 直接或间接检测报道探针补体的合成，作为目标存在的指示。
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公开(公告)号：US20060246495A1
公开(公告)日：2006-11-02
申请号：US11404744
申请日：2006-04-14
申请人： James Garrett , Sha-Sha Wang , Keith Thornton , Richard Moore , William Keating , William Nussbaumer , Craig Whiteford
发明人： James Garrett , Sha-Sha Wang , Keith Thornton , Richard Moore , William Keating , William Nussbaumer , Craig Whiteford
IPC分类号： C12Q1/68 , G01N33/567 , G01N33/53
CPC分类号： C12Q1/6883 , C12Q1/6888 , C12Q2600/112 , C12Q2600/158 , C12Q2600/16 , G01N33/56911 , G01N33/6893 , G01N2333/5412 , G01N2333/5421 , G01N2800/26 , G01N2800/50
摘要： Methods for predicting the development of sepsis in a subject at risk for developing sepsis are provided. In one method, features in a biomarker profile of the subject are evaluated. The subject is likely to develop sepsis if these features satisfy a particular value set. Methods for predicting the development of a stage of sepsis in a subject at risk for developing a stage of sepsis are provided. In one method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to have the stage of sepsis if these feature values satisfy a particular value set. Methods of diagnosing sepsis in a subject are provided. In one such method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to develop sepsis when the plurality of features satisfies a particular value set.
摘要翻译： 提供了预防脓毒症风险发生的患者脓毒症发展的方法。 在一种方法中，评估受试者的生物标志物特征中的特征。 如果这些特征满足特定的值集合，主体可能会发展为败血症。 提供了预防脓毒症发展阶段脓毒症发展阶段发展的方法。 在一种方法中，评估受试者的生物标志物轮廓中的多个特征。 如果这些特征值满足特定的值集合，受试者可能具有脓毒症的阶段。 提供诊断受试者败血症的方法。 在一种这样的方法中，评估对象的生物标志物轮廓中的多个特征。 当多个特征满足特定值集合时，受试者可能发展为败血症。
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公开(公告)号：US07767395B2
公开(公告)日：2010-08-03
申请号：US11404744
申请日：2006-04-14
申请人： James A. Garrett , Sha-Sha Wang , Keith Thornton , Richard L. Moore , William Keating , William A. Nussbaumer , Craig C. Whiteford
发明人： James A. Garrett , Sha-Sha Wang , Keith Thornton , Richard L. Moore , William Keating , William A. Nussbaumer , Craig C. Whiteford
CPC分类号： C12Q1/6883 , C12Q1/6888 , C12Q2600/112 , C12Q2600/158 , C12Q2600/16 , G01N33/56911 , G01N33/6893 , G01N2333/5412 , G01N2333/5421 , G01N2800/26 , G01N2800/50
摘要： Methods for predicting the development of sepsis in a subject at risk for developing sepsis are provided. In one method, features in a biomarker profile of the subject are evaluated. The subject is likely to develop sepsis if these features satisfy a particular value set. Methods for predicting the development of a stage of sepsis in a subject at risk for developing a stage of sepsis are provided. In one method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to have the stage of sepsis if these feature values satisfy a particular value set. Methods of diagnosing sepsis in a subject are provided. In one such method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to develop sepsis when the plurality of features satisfies a particular value set.
摘要翻译： 提供了预防脓毒症风险发生的患者脓毒症发展的方法。 在一种方法中，评估受试者的生物标志物特征中的特征。 如果这些特征满足特定的值集合，主体可能会发展为败血症。 提供了预防脓毒症发展阶段脓毒症发展阶段发展的方法。 在一种方法中，评估受试者的生物标志物轮廓中的多个特征。 如果这些特征值满足特定的值集合，受试者可能具有脓毒症的阶段。 提供诊断受试者败血症的方法。 在一种这样的方法中，评估对象的生物标志物轮廓中的多个特征。 当多个特征满足特定值集合时，受试者可能发展为败血症。
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公开(公告)号：US10190152B2
公开(公告)日：2019-01-29
申请号：US12874602
申请日：2010-09-02
IPC分类号： C12Q1/68 , C12N1/06 , C12Q1/70 , C12Q1/6806 , G01N33/569
摘要： A direct chemical lysis composition includes an assay compatible buffer composition and an assay compatible surfactant. When combined with a specimen storage composition, such compositions prevent undesired modifications to nucleic acid and proteins lysed from cells in the biological sample. Assays of samples from such compositions do not require expensive and time-consuming steps such as centrifugation and prolonged high temperature processing. The direct chemical lysis composition of the present invention permits direct nucleic acid extraction from the cells in the biological sample without the need to decant off the transport media or otherwise exchange the transport media with assay compatible buffers. There is no need to combine the sample with proteinase K or another enzyme to extract nucleic acids from the cells. A method for lysing cells to obtain target nucleic acid for assay and a kit for combining the direct chemical lysis composition with a sample are also contemplated.
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公开(公告)号：US20110105350A1
公开(公告)日：2011-05-05
申请号：US12776245
申请日：2010-05-07
申请人： James A. Garrett , Sha-Sha Wang , Keith Thornton , Richard L. Moore , William Keating , William A. Nussbaumer , Craig C. Whiteford
发明人： James A. Garrett , Sha-Sha Wang , Keith Thornton , Richard L. Moore , William Keating , William A. Nussbaumer , Craig C. Whiteford
CPC分类号： C12Q1/6883 , C12Q1/6888 , C12Q2600/112 , C12Q2600/158 , C12Q2600/16 , G01N33/56911 , G01N33/6893 , G01N2333/5412 , G01N2333/5421 , G01N2800/26 , G01N2800/50
摘要： Methods for predicting the development of sepsis in a subject at risk for developing sepsis are provided. In one method, features in a biomarker profile of the subject are evaluated. The subject is likely to develop sepsis if these features satisfy a particular value set. Methods for predicting the development of a stage of sepsis in a subject at risk for developing a stage of sepsis are provided. In one method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to have the stage of sepsis if these feature values satisfy a particular value set. Methods of diagnosing sepsis in a subject are provided. In one such method, a plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to develop sepsis when the plurality of features satisfies a particular value set.
摘要翻译： 提供了预防脓毒症风险发生的患者脓毒症发展的方法。 在一种方法中，评估受试者的生物标志物特征中的特征。 如果这些特征满足特定的值集合，主体可能会发展为败血症。 提供了预防脓毒症发展阶段脓毒症发展阶段发展的方法。 在一种方法中，评估受试者的生物标志物轮廓中的多个特征。 如果这些特征值满足特定的值集合，受试者可能具有脓毒症的阶段。 提供诊断受试者败血症的方法。 在一种这样的方法中，评估对象的生物标志物轮廓中的多个特征。 当多个特征满足特定值集合时，受试者可能发展为败血症。
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公开(公告)号：US06682889B1
公开(公告)日：2004-01-27
申请号：US09708208
申请日：2000-11-08
申请人： Sha-Sha Wang , David Wolfe
发明人： Sha-Sha Wang , David Wolfe
IPC分类号： C12Q168
CPC分类号： C12Q1/6893
摘要： Amplification primers and methods for specific amplification and detection of a rnpB gene sequence are disclosed. The primer-target binding sequences are useful for amplification and detection of organisms of the Chlamydiaceae family in a variety of amplification and detection reactions.
摘要翻译： 公开了用于特异性扩增和检测rnpB基因序列的扩增引物和方法。 引物 - 靶结合序列可用于在多种扩增和检测反应中扩增和检测衣原体科的生物体。
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公开(公告)号：US20110059455A1
公开(公告)日：2011-03-10
申请号：US12874602
申请日：2010-09-02
CPC分类号： C12Q1/6806 , C12N1/06 , C12Q1/708 , G01N33/56983 , G01N2333/025 , C12Q2527/137 , C12Q2527/125
摘要： A direct chemical lysis composition includes an assay compatible buffer composition and an assay compatible surfactant. When combined with a specimen storage composition, such compositions prevent undesired modifications to nucleic acid and proteins lysed from cells in the biological sample. Assays of samples from such compositions do not require expensive and time-consuming steps such as centrifugation and prolonged high temperature processing. The direct chemical lysis composition of the present invention permits direct nucleic acid extraction from the cells in the biological sample without the need to decant off the transport media or otherwise exchange the transport media with assay compatible buffers. There is no need to combine the sample with proteinase K or another enzyme to extract nucleic acids from the cells. A method for lysing cells to obtain target nucleic acid for assay and a kit for combining the direct chemical lysis composition with a sample are also contemplated.
摘要翻译： 直接化学裂解组合物包括测定相容缓冲液组合物和测定相容性表面活性剂。 当与样品储存组合物组合时，这种组合物防止对生物样品中细胞裂解的核酸和蛋白质的不期望的修饰。 来自这些组合物的样品的测定不需要昂贵且耗时的步骤，例如离心和长时间的高温处理。 本发明的直接化学溶解组合物允许从生物样品中的细胞中直接进行核酸提取，而不需要从运输介质中滗出或以其它方式与测定相容的缓冲液交换传输介质。 不需要将样品与蛋白酶K或其他酶组合从细胞中提取核酸。 还考虑了用于裂解细胞以获得用于测定的靶核酸的方法和用于将直接化学裂解组合物与样品组合的试剂盒。
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