摘要:
The present invention provides methods, kits and compositions for high fidelity nucleic acid amplification reactions of target nucleic acids such that specific amplification products that incorporate non-standard bases are produced. The amplification reactions can either be linear or exponential. In some embodiments, the fidelity of the amplification reaction is high enough such that a majority of the amplification products maintain non-standard bases at specific sites designated by the user after a specified number of amplification cycles. In some embodiments, the ratio of non-standard nucleoside triphosphates is greater than the amount of standard nucleoside triphosphates initially present in the reaction mixture.
摘要:
Disclosed are methods, kits, and components for detecting JAK2 nucleic acids in a sample. In one aspect, the methods may be used to detect mutant JAK2 nucleic acid in a mixture of mutant JAK2 nucleic acid and wild-type JAK2 nucleic acid. The methods utilize primers and reporter molecules comprising non-natural bases. The disclosed kits may include one or more components for performing the disclosed methods.
摘要:
The invention provides methods for selecting nucleotide sequences that yield dsRNA-mediated gene suppression in a target organism and enable their uptake by the target organism. The invention further provides expression constructs that confer stabilized expression of such sequences in a transgenic host cell, and methods for their use. Also provided are organisms, cells and tissues prepared by a method of the invention.
摘要:
Various embodiments of methods and systems for providing virtual leaf driver nodes in a clock tree to drive a clock grid of an integrated circuit are disclosed. An integrated circuit may include a large number of clocked elements such as registers, flip-flops, etc. whose operation is synchronized by one or more clocks. For example, an operation performed by circuitry on one side of the die may need to occur at precisely the same time as another operation performed by circuitry on the other side of the die. In order to assure synchronicity of these events, a clock grid may be provided in the IC that is driven by virtual leaf driver nodes. The clock tree driving the clock grid may include a tier of leaf buffers. The output of a leaf buffer may be split, and the branches of the output connected to separate points on the clock grid.
摘要:
A tool for scraping paint and dusting the loosened particles away. The tool fits in the pocket of the painter's overalls which is designed for the scraper alone, and it eliminates the need for a separate brush or rag to dust a surface clean for painting. The Dusper has snapping pins on the handle for removing and replacing the brush.
摘要:
An article of furniture that is convertible from a couch configuration into a bed configuration. The ability to convert the article is provided by a two pieced frame assembly including a seat section and a back section which are pivotally attached preferably at only two discreet points. The back section is provided with legs that support the back section in a horizontal position when the article is in its bed configuration which, in turn, is attached by pivots to the rear portion of the seat section and supports same. The front portion of the seat section is supported by separate legs.
摘要:
A method and apparatus for powering up an integrated circuit (IC). An IC includes a plurality of power domains each coupled to receive power from one of a plurality of power sources. Each power domain includes a power-sensing unit. A power-sensing unit in a first one of the plurality of power domains is coupled to receive a first power ok signal from an upstream power domain, and is configured to assert a second power ok signal to be provided to a second power domain. A power-sensing unit in the second power domain is coupled to detect the presence of voltage in the first power domain, and to receive the first power ok signal. When the power-sensing unit in the second power domain has both sensed the presence of power in the first power domain and received the second power ok signal, a third power ok signal is asserted.
摘要:
A network abstraction and isolation layer (NAIL) for masquerading the machine identity of a computer in a network to enable the computer to communicate in the network with a different machine identity including an isolated network interface for communicating with the computer, an abstraction network interface for communicating with a network device coupled to the network, and control instructions or device. The control instructions or device performs machine identity translation to masquerade machine identity of the computer relative to the network. Machine identity masquerading includes selectively translating any one or more of an IP address, a MAC address, a machine name, a system identifier, and a DNS Name in the header or payload of communication packets.
摘要:
The present invention provides apparatus and methods for treating the beaks of poultry to prevent further growth by non-contact heating while protecting the birds' tongues. The birds' tongues are protected by providing pressure on underside of the lower beak of the bird, proximate the base of the lower beak.