公开(公告)号：US20140004580A1

公开(公告)日：2014-01-02

申请号：US13995912

申请日：2011-12-19

申请人： James Roberts ,  Fred Cross ,  Brett K. Kaiser

发明人： James Roberts ,  Fred Cross ,  Brett K. Kaiser

IPC分类号： C12P7/64 ,  C12N15/82

CPC分类号： C12P7/6436 ,  C12N9/0008 ,  C12N15/74 ,  C12N15/8242 ,  C12N15/8247 ,  C12P7/6409 ,  C12Y102/0108

摘要： This disclosure describes genetically modified photosynthetic microorganisms, e.g., Cyanobacteria, that overexpress an acyl-acyl carrier protein reductase (acyl-ACP reductase). These microorganisms may optionally overexpress one or more fatty acid synthesis proteins such as ACP and ACCase, and/or one or more polypeptides associated with glycogen breakdown. Also included are photosynthetic microorganisms comprising mutations or deletions in a glycogen biosynthesis or storage pathway, which accumulate a reduced amount of glycogen under reduced nitrogen conditions as compared to a wild type photosynthetic microorganism. The modified photosynthetic microorganisms provided herein are capable of producing increased amounts of lipids such as fatty acids or wax esters and/or synthesizing triglycerides.

摘要翻译： 本公开描述了过表达酰基酰基载体蛋白还原酶（酰基-ACP还原酶）的转基因修饰的光合微生物，例如蓝细菌。 这些微生物可以任选地过表达一种或多种脂肪酸合成蛋白，例如ACP和ACCase，和/或与糖原分解有关的一种或多种多肽。 还包括光合微生物，其包含糖原生物合成或储存途径中的突变或缺失，与野生型光合微生物相比，其在降低的氮条件下积累减少量的糖原。 本文提供的修饰的光合微生物能够产生增加量的脂质，例如脂肪酸或蜡酯和/或合成甘油三酯。

公开(公告)号：US09523096B2

公开(公告)日：2016-12-20

申请号：US13995925

申请日：2011-12-19

申请人： James Roberts ,  Fred Cross ,  Margaret Mary McCormick ,  Ernesto Javier Munoz ,  Brett K. Kaiser ,  Michael Carleton

发明人： James Roberts ,  Fred Cross ,  Margaret Mary McCormick ,  Ernesto Javier Munoz ,  Brett K. Kaiser ,  Michael Carleton

IPC分类号： C12N1/00 ,  C12N1/21 ,  C12N15/82 ,  C07K14/195 ,  C12N9/10 ,  C12N9/12 ,  C12N9/16 ,  C12N9/20 ,  C12N9/90 ,  C12N9/00 ,  C12P7/64

CPC分类号： C12N15/82 ,  C07K14/195 ,  C12N9/1029 ,  C12N9/12 ,  C12N9/16 ,  C12N9/20 ,  C12N9/90 ,  C12N9/93 ,  C12P7/64 ,  Y02P20/52

摘要： This disclosure describes genetically modified photosynthetic microorganisms, e.g., Cyanobacteria, that overexpress an acyl carrier protein (ACP), an acyl-ACP synthase (Aas), or both, optionally in combination with one or more overexpressed or exogenous lipid biosynthesis proteins, and/or one or more overexpressed or exogenous glycogen breakdown proteins. Exemplary biosynthesis proteins include diacyglycerol acyltransferases, thioesterases, phosphatidate phosphatases, phospholipases, triacylglycerol (TAG) hydrolases, fatty acyl-CoA synthetases, and/or acetyl-CoA carboxylases, including combinations thereof. Also included are photosynthetic microorganisms comprising mutations or deletions in a glycogen biosynthesis or storage pathway, which accumulate a reduced amount of glycogen under reduced nitrogen conditions as compared to a wild type photosynthetic microorganism. The modified photosynthetic microorganisms provided herein are capable of producing increased amounts of lipids such as fatty acids and/or synthesizing triglycerides.

摘要翻译： 本公开描述了任选与一种或多种过表达或外源脂质生物合成蛋白组合的转基因修饰的光合微生物，例如过度表达酰基载体蛋白（ACP），酰基-ACP合酶（Aas）或两者的蓝细菌，和/ 或一种或多种过表达或外源糖原分解蛋白。 示例性生物合成蛋白包括二酰甘油酰基转移酶，硫酯酶，磷脂酸磷酸酶，磷脂酶，三酰基甘油（TAG）水解酶，脂肪酰辅酶A合成酶和/或乙酰辅酶A羧化酶，包括其组合。 还包括光合微生物，其包含糖原生物合成或储存途径中的突变或缺失，与野生型光合微生物相比，其在降低的氮条件下积累减少量的糖原。 本文提供的修饰的光合微生物能够产生增加量的脂质，例如脂肪酸和/或合成甘油三酯。

公开(公告)号：US20130344549A1

公开(公告)日：2013-12-26

申请号：US13995925

申请日：2011-12-19

申请人： James Roberts ,  Fred Cross ,  Margaret Mary McCormick ,  Ernesto Javier Munoz ,  Brett K. Kaiser ,  Michael Carleton

发明人： James Roberts ,  Fred Cross ,  Margaret Mary McCormick ,  Ernesto Javier Munoz ,  Brett K. Kaiser ,  Michael Carleton

IPC分类号： C12P7/64 ,  C12N15/82

CPC分类号： C12N15/82 ,  C07K14/195 ,  C12N9/1029 ,  C12N9/12 ,  C12N9/16 ,  C12N9/20 ,  C12N9/90 ,  C12N9/93 ,  C12P7/64 ,  Y02P20/52

摘要： This disclosure describes genetically modified photosynthetic microorganisms, e.g., Cyanobacteria, that overexpress an acyl carrier protein (ACP), an acyl-ACP synthase (Aas), or both, optionally in combination with one or more overexpressed or exogenous lipid biosynthesis proteins, and/or one or more overexpressed or exogenous glycogen breakdown proteins. Exemplary biosynthesis proteins include diacyglycerol acyltransferases, thioesterases, phosphatidate phosphatases, phospholipases, triacylglycerol (TAG) hydrolases, fatty acyl-CoA synthetases, and/or acetyl-CoA carboxylases, including combinations thereof. Also included are photosynthetic microorganisms comprising mutations or deletions in a glycogen biosynthesis or storage pathway, which accumulate a reduced amount of glycogen under reduced nitrogen conditions as compared to a wild type photosynthetic microorganism. The modified photosynthetic microorganisms provided herein are capable of producing increased amounts of lipids such as fatty acids and/or synthesizing triglycerides.

摘要翻译： 本公开描述了任选与一种或多种过表达或外源脂质生物合成蛋白组合的转基因修饰的光合微生物，例如过度表达酰基载体蛋白（ACP），酰基-ACP合酶（Aas）或两者的蓝细菌，和/ 或一种或多种过表达或外源糖原分解蛋白。 示例性生物合成蛋白包括二酰甘油酰基转移酶，硫酯酶，磷脂酸磷酸酶，磷脂酶，三酰基甘油（TAG）水解酶，脂肪酰辅酶A合成酶和/或乙酰辅酶A羧化酶，包括其组合。 还包括光合微生物，其包含糖原生物合成或储存途径中的突变或缺失，与野生型光合微生物相比，其在降低的氮条件下积累减少量的糖原。 本文提供的修饰的光合微生物能够产生增加量的脂质，例如脂肪酸和/或合成甘油三酯。