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    Vascular targets for detecting, imaging and treating neoplasia or neovasculature
    2.
    发明授权
    Vascular targets for detecting, imaging and treating neoplasia or neovasculature 有权
    用于检测，成像和治疗肿瘤或新生血管的血管靶

    公开(公告)号：US08715675B2

    公开(公告)日：2014-05-06

    申请号：US12766062

    申请日：2010-04-23

    申请人： Jan E. Schnitzer ,  Philip Oh

    发明人： Jan E. Schnitzer ,  Philip Oh

    IPC分类号： A61K39/395

    CPC分类号： A61K38/1709 ,  A61K2039/505 ,  C07K16/18

    摘要： Methods of delivering an agent in a tissue-specific manner, by targeting annexin A1, a derivative of annexin A1, or a binding partner of annexin A1, are described. The methods can be used for detecting, imaging and/or treating neoplasia, angiogenesis or neovasculature, as well as for diagnostics and methods of assessing treatment efficacy. Antibodies to annexin A1 are also described, as are methods screening for agents altering annexin A1 activity.

    摘要翻译： 描述了通过靶向膜联蛋白A1，膜联蛋白A1的衍生物或膜联蛋白A1的结合配偶体以组织特异性方式递送药剂的方法。 该方法可用于检测，成像和/或治疗瘤形成，血管生成或新血管系统，以及评估治疗功效的诊断和方法。 还描述了膜联蛋白A1的抗体，以及筛选改变膜联蛋白A1活性的药剂的方法。

    Immunoisolation of caveolae
    3.
    发明授权
    Immunoisolation of caveolae 失效
    穴状病毒的免疫隔离

    公开(公告)号：US06737516B1

    公开(公告)日：2004-05-18

    申请号：US09208195

    申请日：1998-12-09

    申请人： Jan E. Schnitzer ,  Philip Oh

    发明人： Jan E. Schnitzer ,  Philip Oh

    IPC分类号： A23J100

    CPC分类号： G01N33/5308

    摘要： Method of producing purified caveolae are described, in which a sample of interest comprising plasma membranes is subjected to an immunoisolation method in which the sample of interest is incubated with an antibody that is specific for caveolin and which binds to caveolin in its native (oligomeric) state, preferably for a brief time period, and caveolae that are bound to the antibody are separated from other materials in the sample of interest. Purified caveolae produced by the methods, and uses for the purified caveolae, are also described.

    摘要翻译： 描述了产生纯化的细胞膜穴状病毒的方法，其中将包含质膜的感兴趣的样品进行免疫隔离方法，其中将感兴趣的样品与特异于小窝蛋白的抗体一起孵育，并结合其天然（寡聚）的小窝蛋白， 状态，优选短暂的时间段，并且与抗体结合的小室游离体与感兴趣的样品中的其它物质分离。 还描述了通过该方法产生的净化的穴状病毒，以及用于纯化的小窝穴病毒的用途。

    Tissue-specific imaging and therapeutic agents targeting proteins expressed on lung endothelial cell surface
    4.
    发明授权
    Tissue-specific imaging and therapeutic agents targeting proteins expressed on lung endothelial cell surface 有权
    针对在内皮细胞表面表达的蛋白质的组织特异性成像和治疗剂

    公开(公告)号：US08815235B2

    公开(公告)日：2014-08-26

    申请号：US11143114

    申请日：2005-06-02

    申请人： Jan E. Schnitzer ,  Philip Oh

    发明人： Jan E. Schnitzer ,  Philip Oh

    IPC分类号： A61K39/395

    CPC分类号： A61K47/6849

    摘要： Methods of delivering an agent in a tissue-specific manner, particularly lung tissue, by targeting a protein expressed on the endothelial cell surface, are described. The methods can be used for detecting, imaging and/or treating pathologies, as well as for diagnostics.

    摘要翻译： 描述了通过靶向内皮细胞表面上表达的蛋白质以组织特异性方式递送药剂的方法，特别是肺组织。 该方法可用于检测，成像和/或治疗病理学以及诊断。

    Method of recovering endothelial membrane from tissue and applications thereof
    6.
    发明授权
    Method of recovering endothelial membrane from tissue and applications thereof 失效
    鉴定血管内皮细胞疾病特异性表面成分的方法。

    公开(公告)号：US5281700A

    公开(公告)日：1994-01-25

    申请号：US928912

    申请日：1992-08-11

    申请人： Jan E. Schnitzer ,  Bruce S. Jacobson

    发明人： Jan E. Schnitzer ,  Bruce S. Jacobson

    IPC分类号： G01N33/48 ,  A61K35/12 ,  A61K35/44 ,  C07K14/435 ,  G01N1/04 ,  C07K3/12 ,  C07K3/28

    CPC分类号： A61K35/44 ,  Y10S436/813 ,  Y10S530/835 ,  Y10S530/837 ,  Y10S530/839 ,  Y10S530/841 ,  Y10S530/846 ,  Y10S530/849 ,  Y10S530/854 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/255

    摘要： A process is disclosed which makes possible the isolation of the luminal endothelial cell membrane from associated tissue. It is particularly applicable to vasculature, but broadly is applicable to all tissue cavities which are accessible from adjacent perfusable lumens. The method involves the identification of characteristic molecules (primarily proteins and lipids) associated with the luminal surface of the any endothelial membrane in situ by utilizing a novel membrane-isolation scheme to separate the endothelium from associated tissue. In this method, the endothelial luminal plasmalemma of a given organ is coated with colloidal silica by perfusion, a pellicle is formed, the coated area of tissue is excised and the coated plasmalemma fragments are isolated from the cognate homogenate by centrifugation. The isolated plasmalemma attached to the pellicle can then be subjected to biochemical analysis to identify and catalogue molecules characteristic of the endothelial membrane. The method is broadly applicable to any tissue of any organ which is adjacent to a perfusable luminal cavity and which has luminal endothelial membrane exposed to such luminal cavity.

    摘要翻译： 公开了一种可以将腔内皮细胞膜与相关组织分离的方法。 它特别适用于脉管系统，但广泛适用于可从相邻可灌注腔内接近的所有组织腔。 该方法涉及通过利用新的膜分离方案来将内皮与相关组织分离，从而鉴定与原位任何内皮膜的腔表面相关联的特征分子（主要是蛋白质和脂质）。 在这种方法中，给予器官的内皮腔内质膜通过灌注涂覆胶体二氧化硅，形成防护薄膜，切下组织的包被区域，并通过离心从同源匀浆中分离包被的浆细胞片段。 然后可以将附着在防护薄膜组件上的分离的等离子体进行生物化学分析，以鉴定和分类内皮膜特征的分子。 该方法广泛适用于与可灌注腔腔相邻并且具有暴露于这种腔腔的腔内皮细胞膜的任何器官的任何组织。

    Isolation and uses of caveolae
    7.
    发明授权
    Isolation and uses of caveolae 失效

    公开(公告)号：US5914127A

    公开(公告)日：1999-06-22

    申请号：US24952

    申请日：1998-02-17

    申请人： Jan E. Schnitzer

    发明人： Jan E. Schnitzer

    IPC分类号： A61K9/127 ,  A61K38/00 ,  C07K14/47 ,  C07K14/705 ,  C07K16/28 ,  C12N9/22 ,  C12N9/88 ,  A61K31/00 ,  C08G18/00 ,  C12N11/02

    CPC分类号： A61K9/1275 ,  C07K14/47 ,  C07K14/705 ,  C07K16/28 ,  C12N9/22 ,  C12N9/88 ,  A61K38/00 ,  Y10S424/812 ,  Y10S435/82

    摘要： Methods of isolating and purifying caveolae, microdomains of GPI-anchored proteins, and membranes consisting essentially of caveolae associated with microdomains of GPI-anchored proteins from endothelial cell membranes are disclosed. The methods comprise coating a luminal surface of an endothelial cell membrane with an adherent first ionic material by perfusion from a luminal cavity adjacent to the endothelial cell membrane, forming a pellicle by contacting the first ionic material with a second ionic material, and isolating and purifying the pellicle. The pellicle is then processed to isolate the desired cellular component. Caveolae which are substantially free of microdomains of GPI-anchored proteins; microdomains of GPI-anchored proteins which are substantially free of caveolae; and membranes consisting essentially of caveolae, microdomains of GPI-anchored proteins, and caveolae associated with microdomains of GPI-anchored proteins; all of which are substantially free of other cellular elements, are also disclosed.

    Method for identification of disease-specific surface components of vascular endothelial cells
    8.
    发明授权
    Method for identification of disease-specific surface components of vascular endothelial cells 失效
    鉴定血管内皮细胞疾病特异性表面成分的方法

    公开(公告)号：US5587297A

    公开(公告)日：1996-12-24

    申请号：US185433

    申请日：1994-01-24

    申请人： Bruce S. Jacobson ,  Jan E. Schnitzer

    发明人： Bruce S. Jacobson ,  Jan E. Schnitzer

    IPC分类号： G01N33/48 ,  A61K35/12 ,  A61K35/44 ,  C07K14/435 ,  G01N1/04 ,  C12Q1/02 ,  C07K1/18 ,  C07K1/30

    CPC分类号： A61K35/44 ,  Y10S436/813 ,  Y10S530/835 ,  Y10S530/837 ,  Y10S530/839 ,  Y10S530/841 ,  Y10S530/846 ,  Y10S530/849 ,  Y10S530/854 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/255

    摘要： The present invention provides methods for identifying molecular components (e.g. proteins and/or lipids) that are characteristic of vascular endothelia associated with a particular disease and are not also present in (i) normal (non-disease associated) vascular endothelia or (ii) non-disease associated vascular endothelia subjected to altered conditions that accompany the disease but are not unique to the disease. The methods include isolating plasma membrane fractions from disease-associated vascular endothelial cells, from normal (nondisease-associated) vascular endothelial cells and from nondisease-associated vascular endothelial cells subjected to at least one of the altered conditions; purifying and resolving molecular components from these membrane fractions; comparing the components resolved from each of the foregoing vascular endothelial cell sources and identifying components that are present in disease-associated endothelial cells and absent from the normal or otherwise conditioned endothelial cells. The resulting endothelial cell membrane components that are unique to the disease state can be used for diagnostic and/or therapeutic purposes.

    摘要翻译： 本发明提供用于鉴定与特定疾病相关的血管内皮特征的分子组分（例如蛋白质和/或脂质）的方法，并且不存在于（i）正常（非疾病相关）血管内皮中，或（ii） 非疾病相关的血管内皮经历伴随疾病的改变的病症，但不是疾病独有的。 所述方法包括从正常（非病变相关的）血管内皮细胞和经受至少一种改变的病症的非病变相关的血管内皮细胞分离病原相关血管内皮细胞的质膜组分; 从这些膜级分中纯化和分离分子成分; 比较从每个前述血管内皮细胞源分辨的组分和识别存在于疾病相关内皮细胞中并且不存在于正常或其它条件的内皮细胞中的组分。 所产生的疾病状态独特的内皮细胞膜组分可用于诊断和/或治疗目的。

    Tissue-specific monoclonal antibodies
    9.
    发明授权
    Tissue-specific monoclonal antibodies 失效
    组织特异性单克隆抗体

    公开(公告)号：US06255457B1

    公开(公告)日：2001-07-03

    申请号：US09029459

    申请日：1998-06-25

    申请人： Jan E. Schnitzer

    发明人： Jan E. Schnitzer

    IPC分类号： C07K1600

    CPC分类号： A61K9/1275 ,  A61K38/00 ,  C07K14/47 ,  C07K14/705 ,  C07K16/28 ,  C12N9/22 ,  C12N9/88

    摘要： Methods of isolating and purifying caveolae, microdomains of GPI-anchored proteins, and membranes consisting essentially of caveolae associated with microdomains of GPI-anchored proteins from endothelial cell membranes are disclosed. The methods comprise coating a luminal surface of an endothelial cell membrane with an adherent first ionic material (e.g. (cationic) colloidal silica) by perfusion from a luminal cavity adjacent to the endothelial cell membrane, forming a pellicle by contacting the first ionic material with a second ionic material, (e.g. acrylic polymer) and isolating and purifying the pellicle. The pellicle is then processed to isolate the desired cellular component. Caveolae which are substantially free of microdomains of GPI-anchored proteins; microdomains of GPI-anchored proteins which are substantially free of caveolae; and membranes consisting essentially of caveolae, microdomains of GPI-anchored proteins, and caveolae associated with the microdomains of GPI-anchored proteins; all of which are substantially free of other cellular elements are also disclosed.

    摘要翻译： 公开了分离和纯化细胞壁蛋白，GPI锚定蛋白的微区域和基本上由与来自内皮细胞膜的GPI锚定蛋白的微区相关的细胞膜穴样细胞膜的方法。 所述方法包括通过从与内皮细胞膜相邻的腔腔腔灌注灌注具有粘附的第一离子材料（例如（阳离子）胶体二氧化硅）的内皮细胞膜的腔表面，通过使第一离子材料与 第二离子材料（例如丙烯酸聚合物）并分离和纯化防护薄膜。 然后处理防护薄膜组件以分离所需的细胞组分。 基本上没有GPI锚定蛋白的微区域的穴状花序 GPI锚定的蛋白质的微区域基本上不含穴状病毒体; 和基本上由caveolae组成的膜，GPI锚定蛋白质的微区域和与GPI锚定的蛋白质的微区域相关的小窝蛋白; 所有这些都基本上不含其它细胞元素。

    Isolation and uses of caveolae
    10.
    发明授权
    Isolation and uses of caveolae 失效

    公开(公告)号：US5776770A

    公开(公告)日：1998-07-07

    申请号：US582917

    申请日：1996-01-04

    申请人： Jan E. Schnitzer

    发明人： Jan E. Schnitzer

    IPC分类号： A61K9/127 ,  A61K38/00 ,  C07K14/47 ,  C07K14/705 ,  C07K16/28 ,  C12N9/22 ,  C12N9/88 ,  C12N11/02 ,  C08G18/00 ,  C07H1/36

    CPC分类号： A61K9/1275 ,  C07K14/47 ,  C07K14/705 ,  C07K16/28 ,  C12N9/22 ,  C12N9/88 ,  A61K38/00 ,  Y10S424/812 ,  Y10S435/82

    摘要： Methods of isolating and purifying caveolae, microdomains of GPI-anchored proteins, and membranes consisting essentially of caveolae associated with microdomains of GPI-anchored proteins from endothelial cell membranes are disclosed. The methods comprise coating a luminal surface of an endothelial cell membrane with an adherent first ionic material by perfusion from a luminal cavity adjacent to the endothelial cell membrane, forming a pellicle by contacting the first ionic material with a second ionic material, and isolating and purifying the pellicle. The pellicle is then processed to isolate the desired cellular component. Caveolae which are substantially free of microdomains of GPI-anchored proteins; microdomains of GPI-anchored proteins which are substantially free of caveolae; and membranes consisting essentially of caveolae, microdomains of GPI-anchored proteins, and caveolae associated with microdomains of GPI-anchored proteins; all of which are substantially free of other cellular elements, are also disclosed.