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    Method for producing protein
    3.
    发明授权
    Method for producing protein 有权

    公开(公告)号：US10442843B2

    公开(公告)日：2019-10-15

    申请号：US15685053

    申请日：2017-08-24

    申请人： NIPPI, INCORPORATED

    发明人： Tomonori Ueno ,  Yuki Taga ,  Kiyoko Goto ,  Yuko Kaku

    IPC分类号： C07K14/47 ,  C12N15/85 ,  C12P21/00 ,  C12P21/02 ,  C12N15/67 ,  C07K14/705

    摘要： It has been believed that promoting the assembly of polysomes composed of many ribosomes attached to mRNA is very effective for highly efficient protein synthesis. However, the mechanism for p180 protein's capability of promoting polysome formation has been yet to be elucidated.The inventors of the present application newly discovered SF3b4 protein as a protein that specifically interacts with the coiled-coil domain of p180 protein, a responsible region for its capability of promoting polysome formation, and which is capable of promoting mRNA localization to an endoplasmic reticulum (ER). The inventors also found that, in cells capable of highly expressing both p180 protein and a protein promoting mRNA localization to an endoplasmic reticulum (ER) (e.g., SF3b4 protein), the mRNA localization to the endoplasmic reticulum can be significantly elevated so that the secretory capacity in cultured cells can be enhanced. Further, the inventors demonstrated that when a particular nucleotide sequence is inserted into an expression plasmid, SF3b4 protein exhibiting protein expression enhancing ability can be localized onto the endoplasmic reticulum membrane, and the mRNA distribution in polysomes can be shifted towards heavier fractions, whereby the secretory capacity in cells can be enhanced.

    Method for producing protein
    4.
    发明授权
    Method for producing protein 有权

    公开(公告)号：US09884897B2

    公开(公告)日：2018-02-06

    申请号：US14780313

    申请日：2014-03-26

    申请人： NIPPI, INCORPORATED

    发明人： Tomonori Ueno ,  Yuki Taga ,  Kiyoko Goto ,  Yuko Kaku

    IPC分类号： C07K14/47 ,  C12P21/02 ,  C12N15/67 ,  C07K14/705 ,  C12N15/85 ,  C12P21/00

    CPC分类号： C07K14/47 ,  C07K14/70553 ,  C12N15/67 ,  C12N15/85 ,  C12P21/00 ,  C12P21/02

    摘要： It has been believed that promoting the assembly of polysomes composed of many ribosomes attached to mRNA is very effective for highly efficient protein synthesis. However, the mechanism for p180 protein's capability of promoting polysome formation has been yet to be elucidated.The inventors of the present application newly discovered SF3b4 protein as a protein that specifically interacts with the coiled-coil domain of p180 protein, a responsible region for its capability of promoting polysome formation, and which is capable of promoting mRNA localization to an endoplasmic reticulum (ER). The inventors also found that, in cells capable of highly expressing both p180 protein and a protein promoting mRNA localization to an endoplasmic reticulum (ER) (e.g., SF3b4 protein), the mRNA localization to the endoplasmic reticulum can be significantly elevated so that the secretory capacity in cultured cells can be enhanced. Further, the inventors demonstrated that when a particular nucleotide sequence is inserted into an expression plasmid, SF3b4 protein exhibiting protein expression enhancing ability can be localized onto the endoplasmic reticulum membrane, and the mRNA distribution in polysomes can be shifted towards heavier fractions, whereby the secretory capacity in cells can be enhanced.

    METHOD FOR PRODUCING PROTEIN
    5.
    发明申请
    METHOD FOR PRODUCING PROTEIN 有权
    生产蛋白质的方法

    公开(公告)号：US20160075747A1

    公开(公告)日：2016-03-17

    申请号：US14780313

    申请日：2014-03-26

    申请人： NIPPI, INCORPORATED

    发明人： Tomonori Ueno ,  Yuki Taga ,  Kiyoko Goto ,  Yuko Kaku

    IPC分类号： C07K14/47 ,  C12P21/00 ,  C12N15/85

    CPC分类号： C07K14/47 ,  C07K14/70553 ,  C12N15/67 ,  C12N15/85 ,  C12P21/00 ,  C12P21/02

    摘要： It has been believed that promoting the assembly of polysomes composed of many ribosomes attached to mRNA is very effective for highly efficient protein synthesis. However, the mechanism for p180 protein's capability of promoting polysome formation has been yet to be elucidated.The inventors of the present application newly discovered SF3b4 protein as a protein that specifically interacts with the coiled-coil domain of p180 protein, a responsible region for its capability of promoting polysome formation, and which is capable of promoting mRNA localization to an endoplasmic reticulum (ER). The inventors also found that, in cells capable of highly expressing both p180 protein and a protein promoting mRNA localization to an endoplasmic reticulum (ER) (e.g., SF3b4 protein), the mRNA localization to the endoplasmic reticulum can be significantly elevated so that the secretory capacity in cultured cells can be enhanced. Further, the inventors demonstrated that when a particular nucleotide sequence is inserted into an expression plasmid, SF3b4 protein exhibiting protein expression enhancing ability can be localized onto the endoplasmic reticulum membrane, and the mRNA distribution in polysomes can be shifted towards heavier fractions, whereby the secretory capacity in cells can be enhanced.

    摘要翻译： 已经认为，促进由连接到mRNA的许多核糖体组成的多核糖体的组装对于高效的蛋白质合成是非常有效的。 然而，p180蛋白质促进多聚体形成能力的机制尚未阐明。 本申请的发明人新发现的SF3b4蛋白作为与p180蛋白的卷曲螺旋结构域特异性相互作用的蛋白质，其是促进多聚体形成的能力的负责区域，并且能够促进mRNA定位于内质网（ ER）。 发明人还发现，在能够高度表达p180蛋白和蛋白质促进mRNA定位于内质网（ER）（例如，SF3b4蛋白）的细胞中）的细胞中，可以显着提高对内质网的mRNA定位，使得分泌 培养细胞的能力可以提高。 此外，本发明人发现，当将特定的核苷酸序列插入表达质粒时，表现出蛋白表达增强能力的SF3b4蛋白可以定位于内质网膜上，并且多核糖体中的mRNA分布可以向较重部分转移，由此分泌 细胞中的能力可以提高。