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    Automated immunoassay cassette, apparatus and method
    5.
    发明授权
    Automated immunoassay cassette, apparatus and method 有权
    自动免疫测定盒，仪器和方法

    公开(公告)号：US07238519B2

    公开(公告)日：2007-07-03

    申请号：US11016016

    申请日：2004-12-17

    申请人： Neal F. Bellet ,  Jeffrey Shindelman ,  Thomas E. Worthy ,  Kimberly Haley ,  Richard K-T Lee ,  Jane Eunhee Kyung ,  Suyue Qian

    发明人： Neal F. Bellet ,  Jeffrey Shindelman ,  Thomas E. Worthy ,  Kimberly Haley ,  Richard K-T Lee ,  Jane Eunhee Kyung ,  Suyue Qian

    IPC分类号： C12M1/34

    CPC分类号： G01N21/03 ,  B01L3/5023 ,  B01L9/52 ,  B01L2200/0668 ,  B01L2300/0825 ,  B01L2300/0887 ,  B01L2400/0406 ,  B01L2400/0633 ,  G01N33/54306 ,  G01N33/54366 ,  G01N33/54386 ,  G01N2035/00108 ,  G01N2035/00128 ,  G01N2035/0436 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S435/97 ,  Y10S436/81

    摘要： An immunoassay cassette, apparatus, and method for detecting an analyte in a liquid body-fluid sample are disclosed. The cassette has a body and a support mounted on the body, for movement relative to the body to first and second transfer positions. Sample supplied to a sample well in the cassette body is transferred to a reagent reservoir on the cassette body, by moving the support to its first transfer position. Here the sample reacts with a first reagent composition in the reservoir effective to form a modified sample. The modified sample is then transferred to a reagent strip on the support by moving the support to its second transfer position. The reagent strip has a transfer zone at which sample material is transferred to the strip, a second reagent composition effective to react with the modified sample to form a detectable analyte-dependent product, and a detection zone located downstream at which the detectable product can be observed. By controlling movement of the support to its first and second positions, the cassette can effectively handle a variety of samples having different flow rates.

    摘要翻译： 公开了用于检测液体体液样品中的分析物的免疫测定盒，装置和方法。 盒具有主体和安装在主体上的支撑件，用于相对于主体移动到第一和第二转移位置。 通过将支撑件移动到其第一转移位置，提供给盒体中的样品阱的样品被转移到盒体上的试剂容器。 这里样品与存储器中的第一试剂组合物反应，有效地形成修饰的样品。 然后通过将支撑件移动到其第二转移位置，将改性样品转移到支撑体上的试剂条。 试剂条具有将样品材料转移到条带上的转移区域，有效与经修饰的样品反应以形成可检测的分析物依赖产物的第二试剂组合物，以及位于下游的检测区域，可检测产物可以 观察到的。 通过控制支撑件到其第一和第二位置的移动，盒可以有效地处理具有不同流速的各种样品。

    Automated immunoassay cassette, apparatus and method
    6.
    发明授权
    Automated immunoassay cassette, apparatus and method 有权
    自动免疫测定盒，仪器和方法

    公开(公告)号：US07220595B2

    公开(公告)日：2007-05-22

    申请号：US10456230

    申请日：2003-06-06

    申请人： Anthony J. Nugent ,  Leanne M. Cowley ,  Neal F. Bellet ,  Jeffrey Shindelman ,  Michael E. Leos ,  Thomas E. Worthy ,  Kimberly Haley

    发明人： Anthony J. Nugent ,  Leanne M. Cowley ,  Neal F. Bellet ,  Jeffrey Shindelman ,  Michael E. Leos ,  Thomas E. Worthy ,  Kimberly Haley

    IPC分类号： G01N33/558

    CPC分类号： B01L9/52 ,  B01L3/5023 ,  B01L2300/0825 ,  B01L2300/0887 ,  B01L2400/0406 ,  B01L2400/0633 ,  G01N33/5304 ,  G01N33/54306 ,  G01N33/54366 ,  G01N33/54386 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S435/97 ,  Y10S436/81

    摘要： An immunoassay cassette, apparatus, and method for detecting an analyte in a liquid body-fluid sample are disclosed. The cassette has a body and a support mounted on the body, for movement toward and away from a sample transfer position. Sample supplied to a sample well in the cassette body is taken up by a reagent reservoir containing a first reagent composition effective to form a modified sample. The support provides a reagent strip having a transfer zone that is brought into contact with the reservoir, when the support is in the transfer position, a detection zone located downstream of the transfer zone, and a second reagent composition effective to react with the modified sample to form a detectable analyte-dependent product. By controlling movement of the support, volume and rate of sample flow from the reservoir to the strip can be controlled to optimize and/or standardize sample-transfer conditions in the assay.

    摘要翻译： 公开了用于检测液体体液样品中的分析物的免疫测定盒，装置和方法。 盒具有安装在主体上的主体和支撑件，用于朝向和远离样品转移位置移动。 在盒体中提供给样品阱的样品被含有有效形成改性样品的第一试剂组合物的试剂容器吸收。 所述支持体提供具有转移区域的试剂条带，所述转移区域在所述载体处于转移位置时与所述储存器接触，位于所述转移区域下游的检测区域和有效与所述修饰样品反应的第二试剂组合物 以形成可检测的分析物依赖性产物。 通过控制载体的移动，可以控制从储存器到条带的样品流量的体积和速率，以在测定中优化和/或标准化样品转移条件。

    Heterogeneous fluorescence assays using controlled pore glass particles
    7.
    发明授权
    Heterogeneous fluorescence assays using controlled pore glass particles 失效
    使用受控孔隙玻璃颗粒的异质荧光测定

    公开(公告)号：US4780423A

    公开(公告)日：1988-10-25

    申请号：US939903

    申请日：1986-12-09

    申请人： Barry I. Bluestein ,  A. Judy Famulare ,  Thomas E. Worthy

    发明人： Barry I. Bluestein ,  A. Judy Famulare ,  Thomas E. Worthy

    IPC分类号： G01N33/543 ,  G01N33/552 ,  G01N33/58

    CPC分类号： G01N33/54313 ,  G01N33/552 ,  G01N33/582 ,  Y10S436/80

    摘要： Improved heterogeneous fluorescence assays are provided which employ solid supports comprising controlled pore glass particles which are substantially transparent at both the wavelength used to excite the fluorescent probe used in the assay and at the emission wavelength of the probe. The assays achieve sensitivities comparable to those achieved with radioimmunoassays without the need to concentrate the particles prior to the fluorescent measurement as in prior art heterogeneous fluorescence assays employing other types of solid supports.

    摘要翻译： 提供了改进的异质荧光测定法，其使用包括受控孔玻璃颗粒的固体支持物，其在用于激发测定中使用的荧光探针的波长和探针的发射波长处都是基本透明的。 该测定实现了与使用放射免疫测定实现的那些相当的灵敏度，而不需要在荧光测量之前浓缩颗粒，如在使用其它类型的固体支持物的现有技术的异质荧光测定中那样。