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公开(公告)号：US20070169226A1
公开(公告)日：2007-07-19
申请号：US11621465
申请日：2007-01-09
申请人： Jeffrey Habben , Chris Zinselmeier , Dwight Tomes , Shane Abbitt , Timothy Helentjaris , Xiaomu Niu
发明人： Jeffrey Habben , Chris Zinselmeier , Dwight Tomes , Shane Abbitt , Timothy Helentjaris , Xiaomu Niu
CPC分类号： C12N15/8234 , C12N9/0032 , C12N9/1085 , C12N15/8243 , C12N15/8261 , C12N15/8271 , C12N15/8295 , Y02A40/146
摘要： This invention relates generally to the field of plant molecular biology. More specifically, this invention relates to methods and reagents for the temporally- and/or spatially-regulated expression of genes, particularly in plant seeds and related female reproductive tissue. The present invention also provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions comprise novel nucleotide sequences for a seed-preferred promoter known as eep1. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell.
摘要翻译： 本发明一般涉及植物分子生物学领域。 更具体地，本发明涉及用于时间和/或空间调节基因表达的方法和试剂，特别是在植物种子和相关的雌性生殖组织中。 本发明还提供用于调节植物中异源核苷酸序列表达的组合物和方法。 组合物包含用于称为eep1的种子优选启动子的新型核苷酸序列。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括转化植物细胞以包含与本发明的启动子之一可操作地连接的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。
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公开(公告)号：US20050229274A1
公开(公告)日：2005-10-13
申请号：US11139461
申请日：2005-05-27
申请人： Jeffrey Habben , Chris Zinselmeier , Dwight Tomes
发明人： Jeffrey Habben , Chris Zinselmeier , Dwight Tomes
CPC分类号： C12N15/8261 , C12N15/8243 , Y02A40/146
摘要： This invention relates generally to the field of plant molecular biology. More specifically, this invention relates to methods and reagents for the temporal and/or spatial expression of genes that affect metabolically effective levels of cytokinins in plant seeds and related maternal tissue. This invention further relates to transgenic plants having enhanced levels of cytokinin expression wherein the transgenic plant exhibits useful characteristics, including: improved seed size, decreased tip kernel abortion, increased seed set during unfavorable environmental conditions, and stability of yield.
摘要翻译： 本发明一般涉及植物分子生物学领域。 更具体地，本发明涉及用于影响植物种子和相关母体组织中细胞分裂素的代谢有效水平的基因的时间和/或空间表达的方法和试剂。 本发明还涉及具有增强的细胞分裂素表达水平的转基因植物，其中转基因植物表现出有用的特征，包括：在不利的环境条件下提高种子大小，降低的穗粒子流产，增加的种子集合以及产量的稳定性。
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3.发明授权Plants with altered root architecture, related constructs and methods involving genes encoding leucine rich repeat kinase (LLRK) polypeptides and homologs thereof 有权具有改变的根结构的植物，涉及编码富含亮氨酸重复激酶（LLRK）多肽的基因的相关构建体和方法及其同源物公开(公告)号：US09090901B2
公开(公告)日：2015-07-28
申请号：US13347850
申请日：2012-01-11
申请人： Graziana Taramino , Stephen M. Allen , Stanley Luck , Xiaomu Niu , Hajime Sakai , Scott V. Tingey , Dwight Tomes
发明人： Graziana Taramino , Stephen M. Allen , Stanley Luck , Xiaomu Niu , Hajime Sakai , Scott V. Tingey , Dwight Tomes
IPC分类号： C12N15/63 , C12N15/82 , C07K14/415
CPC分类号： C12N15/8262 , C07K14/415 , C12N9/12 , C12N15/8201 , C12N15/8241 , C12Y207/11001
摘要： Isolated polynucleotides and polypeptides and recombinant DNA constructs particularly useful for altering root structure of plants, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter functional in a plant, wherein said polynucleotide encodes a polypeptide useful for altering plant root architecture.
摘要翻译： 分离的多核苷酸和多肽以及特别可用于改变植物的根结构，包含这些重组DNA构建体的组合物（例如植物或种子）以及利用这些重组DNA构建体的方法的重组DNA构建体。 重组DNA构建体包含可操作地连接到在植物中起作用的启动子的多核苷酸，其中所述多核苷酸编码可用于改变植物根结构的多肽。
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4.发明申请PLANTS WITH ALTERED ROOT ARCHITECTURE, RELATED CONSTRUCTS AND METHODS INVOLVING GENES ENCODING EXOSTOSIN FAMILY POLYPEPTIDES AND HOMOLOGS THEREOF 审中-公开具有改变的根架构的植物，涉及除草剂家族多肽及其同源物的基因的相关构造和方法公开(公告)号：US20110035822A1
公开(公告)日：2011-02-10
申请号：US12908397
申请日：2010-10-20
申请人： Graziana Taramino , Hajime Sakai , Scott V. Tingey , Stephen M. Allen , Stanley Luck , Dwight Tomes , Xiaomu Niu
发明人： Graziana Taramino , Hajime Sakai , Scott V. Tingey , Stephen M. Allen , Stanley Luck , Dwight Tomes , Xiaomu Niu
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/8201 , C12N15/8271 , Y02A40/146
摘要： Isolated polynucleotides and polypeptides and recombinant DNA constructs particularly useful for altering root structure of plants, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter functional in a plant, wherein said polynucleotide encodes a polypeptide useful for altering plant root architecture.
摘要翻译： 分离的多核苷酸和多肽以及特别可用于改变植物的根结构，包含这些重组DNA构建体的组合物（例如植物或种子）以及利用这些重组DNA构建体的方法的重组DNA构建体。 重组DNA构建体包含可操作地连接到在植物中起作用的启动子的多核苷酸，其中所述多核苷酸编码可用于改变植物根结构的多肽。
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5.发明授权公开(公告)号：US07479584B2
公开(公告)日：2009-01-20
申请号：US11190339
申请日：2005-07-27
申请人： Dwight Tomes , Silvio Salvi , Michele Morgante , Giorgio Sponza , Edward Bruggemann , Xiaomu Niu , Bailin Li , Roberto Tuberosa
发明人： Dwight Tomes , Silvio Salvi , Michele Morgante , Giorgio Sponza , Edward Bruggemann , Xiaomu Niu , Bailin Li , Roberto Tuberosa
CPC分类号： C12N15/827 , C07K14/415
摘要： The present invention provides compositions and methods for modulating flowering time in plants. Maize RAP2.7 nucleotide sequences are disclosed which upon overexpression cause later flowering and when inhibited cause earlier flowering. Also disclosed is a DNA sequence which acts as a regulator/enhancer of RAP2.7, termed VGT1. This sequence does not code for any known protein, but acts as either a RNAi element or a regulatory DNA or RNA element that either directly regulates expression of flowering genes such as Rap2.7 or specifically targets expression of other genes which control flowering genes such as Rap2.7. This element this can be used as a sequence—based marker to identify inbred and hybrids which have altered maturity. Methods for expressing these nucleotide sequences in a plant for modifying maturity and flowering in plants are provided as well as expression constructs, vectors, transformed cells and plants.
摘要翻译： 本发明提供调节植物开花时间的组合物和方法。 公开了玉米RAP2.7核苷酸序列，其在过表达导致后期开花并且当被抑制时引起早期开花。 还公开了作为RAP2.7调节/增强子的DNA序列，称为VGT1。 该序列不编码任何已知的蛋白质，而是作为直接调节开花基因如Rap2.7的表达的RNAi元件或调节DNA或RNA元件，或者特异性靶向控制开花基因的其他基因的表达，例如 Rap2.7。 这个元素可以用作基于序列的标记来鉴定已经改变成熟度的近交系和杂交种。 提供用于在植物中表达这些核苷酸序列以修饰植物中的成熟和开花的方法以及表达构建体，载体，转化的细胞和植物。
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6.发明申请PLANTS WITH ALTERED ROOT ARCHITECTURE, RELATED CONSTRUCTS AND METHODS INVOLVING GENES ENCODING NUCLEOSIDE DIPHOSPHATASE KINASE (NDK) POLYPEPTIDES AND HOMOLOGS THEREOF 审中-公开具有改变的根结构的植物，涉及编码核苷酸二磷酸酶激酶（NDK）多肽的基因的相关构建体和方法及其同源物公开(公告)号：US20120124697A1
公开(公告)日：2012-05-17
申请号：US12671808
申请日：2008-08-29
申请人： Graziana Taramino , Scott V. Tingey , Stephen M. Allen , Dwight Tomes , Stanley Luck , Hajime Sakai , Xiaomu Niu
发明人： Graziana Taramino , Scott V. Tingey , Stephen M. Allen , Dwight Tomes , Stanley Luck , Hajime Sakai , Xiaomu Niu
CPC分类号： C12N15/8273 , C12N9/1229 , C12N15/8262
摘要： Isolated polynucleotides and polypeptides and recombinant DNA constructs particularly useful for altering root structure of plants, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter functional in a plant, wherein said polynucleotide encodes a polypeptide useful for altering plant root architecture.
摘要翻译： 分离的多核苷酸和多肽以及特别可用于改变植物的根结构，包含这些重组DNA构建体的组合物（例如植物或种子）以及利用这些重组DNA构建体的方法的重组DNA构建体。 重组DNA构建体包含可操作地连接到在植物中起作用的启动子的多核苷酸，其中所述多核苷酸编码可用于改变植物根结构的多肽。
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公开(公告)号：US20050204432A1
公开(公告)日：2005-09-15
申请号：US10931077
申请日：2004-08-31
申请人： Dilbag Multani , Xiaomu Niu , Dwight Tomes , Haiyin Wang , Deping Xu
发明人： Dilbag Multani , Xiaomu Niu , Dwight Tomes , Haiyin Wang , Deping Xu
CPC分类号： C07K14/415 , C12N15/8222 , C12N15/8223
摘要： The invention relates to the regulation of gene expression, particularly to methods and compositions for regulating gene expression in plants. Expression cassettes comprising a polynucleotide molecule of interest operably linked to the Brachytic2 (Br2) promoter, the Dwarf3 (Dw3) promoter, or a functional fragment or variant thereof, are provided. Methods of using such expression cassettes, as well as plant cells, plant tissues, plants, and seeds transformed with these expression cassettes, are additionally provided. The methods and compositions find use in regulating the expression of a polynucleotide molecule of interest in a plant.
摘要翻译： 本发明涉及基因表达的调节，特别涉及用于调节植物中基因表达的方法和组合物。 提供了包含与Brachytic2（Br2）启动子，Dw3（Dw3）启动子或其功能片段或变体可操作连接的目的多核苷酸分子的表达盒。 另外提供了使用这些表达盒以及用这些表达盒转化的植物细胞，植物组织，植物和种子的方法。 所述方法和组合物用于调节植物中感兴趣的多核苷酸分子的表达。
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8.发明授权Plants with altered root architecture, related constructs and methods involving genes encoding exostosin family polypeptides and homologs thereof 有权具有改变的根结构的植物，涉及编码外瘤蛋白家族多肽的基因的相关构建体和方法及其同源物公开(公告)号：US09115203B2
公开(公告)日：2015-08-25
申请号：US12908397
申请日：2010-10-20
申请人： Graziana Taramino , Hajime Sakai , Scott V Tingey , Stanley Luck , Dwight Tomes , Xiaomu Niu
发明人： Graziana Taramino , Hajime Sakai , Scott V Tingey , Stanley Luck , Dwight Tomes , Xiaomu Niu
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/8201 , C12N15/8271 , Y02A40/146
摘要： Isolated polynucleotides and polypeptides and recombinant DNA constructs particularly useful for altering root structure of plants, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter functional in a plant, wherein said polynucleotide encodes a polypeptide useful for altering plant root architecture.
摘要翻译： 分离的多核苷酸和多肽以及特别可用于改变植物的根结构，包含这些重组DNA构建体的组合物（例如植物或种子）以及利用这些重组DNA构建体的方法的重组DNA构建体。 重组DNA构建体包含可操作地连接到在植物中起作用的启动子的多核苷酸，其中所述多核苷酸编码可用于改变植物根结构的多肽。
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9.发明申请PLANTS WITH ALTERED ROOT ARCHITECTURE, RELATED CONSTRUCTS AND METHODS INVOLVING GENES ENCODING LEUCINE RICH REPEAT KINASE (LLRK) POLYPEPTIDES AND HOMOLOGS THEREOF 审中-公开具有改变的根部结构的植物，涉及基因的相关构造和方法编码多种重复激酶（LLRK）多肽及其同源物公开(公告)号：US20120110700A1
公开(公告)日：2012-05-03
申请号：US13347850
申请日：2012-01-11
申请人： Graziana Taramino , Scott V. Tingey , Hajime Sakai , Stephen M. Allen , Dwight Tomes , Stanley Luck , Xiaomu Niu
发明人： Graziana Taramino , Scott V. Tingey , Hajime Sakai , Stephen M. Allen , Dwight Tomes , Stanley Luck , Xiaomu Niu
CPC分类号： C12N15/8262 , C07K14/415 , C12N9/12 , C12N15/8201 , C12N15/8241 , C12Y207/11001
摘要： Isolated polynucleotides and polypeptides and recombinant DNA constructs particularly useful for altering root structure of plants, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter functional in a plant, wherein said polynucleotide encodes a polypeptide useful for altering plant root architecture.
摘要翻译： 分离的多核苷酸和多肽以及特别可用于改变植物的根结构，包含这些重组DNA构建体的组合物（例如植物或种子）以及利用这些重组DNA构建体的方法的重组DNA构建体。 重组DNA构建体包含可操作地连接到在植物中起作用的启动子的多核苷酸，其中所述多核苷酸编码可用于改变植物根结构的多肽。
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10.发明申请PLANTS WITH ALTERED ROOT ARCHITECTURE, RELATED CONSTRUCTS AND METHODS INVOLVING GENES ENCODING EXOSTOSIN FAMILY POLYPEPTIDES AND HOMOLOGS THEREOF 审中-公开具有改变的根架构的植物，涉及除草剂家族多肽及其同源物的基因的相关构造和方法公开(公告)号：US20090113570A1
公开(公告)日：2009-04-30
申请号：US12261411
申请日：2008-10-30
申请人： Graziana Taramino , Hajime Sakai , Scott V. Tingey , Stephen M. Allen , Stanley Luck , Dwight Tomes , Xiaomu Niu
发明人： Graziana Taramino , Hajime Sakai , Scott V. Tingey , Stephen M. Allen , Stanley Luck , Dwight Tomes , Xiaomu Niu
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/8201 , C12N15/8271 , Y02A40/146
摘要： Isolated polynucleotides and polypeptides and recombinant DNA constructs particularly useful for altering root structure of plants, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter functional in a plant, wherein said polynucleotide encodes a polypeptide useful for altering plant root architecture.
摘要翻译： 分离的多核苷酸和多肽以及特别可用于改变植物的根结构，包含这些重组DNA构建体的组合物（例如植物或种子）以及利用这些重组DNA构建体的方法的重组DNA构建体。 重组DNA构建体包含可操作地连接到在植物中起作用的启动子的多核苷酸，其中所述多核苷酸编码可用于改变植物根结构的多肽。
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