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    Iterative optical based histology
    3.
    发明申请
    Iterative optical based histology 审中-公开
    迭代光学基础组织学

    公开(公告)号：US20050035305A1

    公开(公告)日：2005-02-17

    申请号：US10886940

    申请日：2004-07-08

    申请人： David Kleinfeld ,  Jeffrey Squier ,  Philbert Tsai

    发明人： David Kleinfeld ,  Jeffrey Squier ,  Philbert Tsai

    IPC分类号： G01N1/04 ,  G01N1/06 ,  G01N33/483 ,  G01N1/00

    CPC分类号： G01N1/06 ,  G01N2001/045 ,  G01N2001/2886

    摘要： Femtosecond laser pulses are used to iteratively cut and image fixed as well as exsanguinated fresh tissue. Such images help to automate three-dimensional histological analysis of biological tissue. Cuts are accomplished with approximately 0.3 to 100 microJoule pulses to ablate tissue with one-micrometer precision. Permeability, immunoreactivity, and optical clarity of the remaining tissue is retained after pulsed laser cutting. Samples from transgenic mice that express fluorescent proteins retained their fluorescence to within micrometers of the cut surface.

    摘要翻译： 飞秒激光脉冲用于迭代切割和图像固定以及放血的新鲜组织。 这样的图像有助于自动化生物组织的三维组织学分析。 用大约0.3至100微焦耳脉冲完成切割，以一微米精度烧蚀组织。 脉冲激光切割后，剩余组织的渗透性，免疫反应性和光学透明度得以保留。 来自表达荧光蛋白的转基因小鼠的样品将其荧光保留在切割表面的微米内。

    Device and method for inducing vascular injury and/or blockage in an animal model
    4.
    发明申请
    Device and method for inducing vascular injury and/or blockage in an animal model 失效
    用于在动物模型中诱导血管损伤和/或阻塞的装置和方法

    公开(公告)号：US20060142746A1

    公开(公告)日：2006-06-29

    申请号：US10538548

    申请日：2003-12-11

    申请人： Beth Friedman ,  David Kleinfeld ,  Patrick Lyden ,  Nozomi Nishimura ,  Christopher Schaffer ,  Lee Schroeder ,  Philbert Tsai

    发明人： Beth Friedman ,  David Kleinfeld ,  Patrick Lyden ,  Nozomi Nishimura ,  Christopher Schaffer ,  Lee Schroeder ,  Philbert Tsai

    IPC分类号： A61B18/18

    CPC分类号： A61B18/20 ,  A61B18/203 ,  A61B2017/00057 ,  A61B2018/00452

    摘要： Ultrashort laser pulses are used to induce photodisruptive breakdown in vasculature in an animal to controllably produce hemorrhage, thrombosis or breach of the blood-brain barrier in individual, specifically-targeted blood vessels. Damage is limited to the targeted vessels such that neighboring vessels exhibit no signs of vascular damage, including vessels directly above and directly below the targeted vessel. Ultrashort laser pulses of lower energy are also used to observe and quantify the baseline and altered states of blood flow. Observation and measurement may be performed by TPLSM, OCT or other known techniques, providing a real-time, in vivo model for the dynamics and effects of vascular injury.

    摘要翻译： 超短脉冲激光脉冲被用于诱发动物脉管系统中的光破坏性破坏，以可控地产生出血，血栓形成或破坏个体特异性靶向血管中的血脑屏障。 损害局限于目标船只，使相邻船只没有血管损伤迹象，包括直接位于目标船只正下方的血管。 较低能量的超短脉冲激光脉冲也用于观察和定量血流的基线和改变的状态。 观察和测量可以通过TPLSM，OCT或其他已知技术进行，为血管损伤的动力学和影响提供实时的体内模型。

    Method for measuring neurotransmitters in vivo
    6.
    发明授权
    Method for measuring neurotransmitters in vivo 有权
    体内神经递质测定方法

    公开(公告)号：US08663604B2

    公开(公告)日：2014-03-04

    申请号：US12676912

    申请日：2008-09-04

    申请人： Quoc-Thang Nguyen ,  David Kleinfeld ,  Lee F. Schroeder

    发明人： Quoc-Thang Nguyen ,  David Kleinfeld ,  Lee F. Schroeder

    IPC分类号： A61B5/00 ,  A61B5/1495

    CPC分类号： G01N33/9406 ,  A61B5/0084 ,  A61B5/4064

    摘要： A method is provided for in vivo detection of a biochemical substance in an animal by culturing neurofluocytes that stably express a receptor of the biochemical substances by transfecting cells with cDNA of the receptor and a tag that will emit a detectable energy in the presence of the biochemical substance, implanting the neurofluocyte into the animal's brain; and detecting the energy emission of the tag. In a first embodiment, the biochemical substance is a neurotransmitter, the tag is a fluophore, and the step of detecting includes forming an opening in the animal's skull and optically detecting fluorescent emissions using a two-photon laser scanning microscope. Multiple biochemical substances can be simultaneously detected by culturing neurofluocytes that express different receptors and have different fluophor tags that produce fluorescent signals at distinguishable wavelengths.

    摘要翻译： 提供一种用于体内检测动物体内生物化学物质的方法，该方法是通过培养通过用受体的cDNA转染细胞来稳定表达生物化学物质的受体的神经细胞，以及在生物化学物质存在下将发出可检测的能量的标签 物质，将神经细胞植入动物的脑中; 并检测标签的能量发射。 在第一实施方案中，生物化学物质是神经递质，标签是荧光团，检测步骤包括在动物颅骨中形成开口，并使用双光子激光扫描显微镜光学检测荧光发射。 可以通过培养表达不同受体的神经细胞同时检测多种生物化学物质，并具有不同的荧光标记，其在可区分的波长处产生荧光信号。

    Method for Measuring Neurotransmitters in Vivo
    7.
    发明申请
    Method for Measuring Neurotransmitters in Vivo 有权
    测量体内神经递质的方法

    公开(公告)号：US20100278746A1

    公开(公告)日：2010-11-04

    申请号：US12676912

    申请日：2008-09-04

    申请人： Quoc-Thang Nguyen ,  David Kleinfeld ,  Lee F. Schroeder

    发明人： Quoc-Thang Nguyen ,  David Kleinfeld ,  Lee F. Schroeder

    IPC分类号： A61K49/00

    CPC分类号： G01N33/9406 ,  A61B5/0084 ,  A61B5/4064

    摘要： A method is provided for in vivo detection of a biochemical substance in an animal by culturing neurofluocytes that stably express a receptor of the biochemical substances by transfecting cells with cDNA of the receptor and a tag that will emit a detectable energy in the presence of the biochemical substance, implanting the neurofluocyte into the animal's brain; and detecting the energy emission of the tag. In a first embodiment, the biochemical substance is a neurotransmitter, the tag is a fluophore, and the step of detecting includes forming an opening in the animal's skull and optically detecting fluorescent emissions using a two-photon laser scanning microscope. Multiple biochemical substances can be simultaneously detected by culturing neurofluocytes that express different receptors and have different fluophor tags that produce fluorescent signals at distinguishable wavelengths.

    摘要翻译： 提供一种用于体内检测动物体内生物化学物质的方法，该方法是通过培养通过用受体的cDNA转染细胞来稳定表达生物化学物质的受体的神经细胞，以及在生物化学物质存在下将发出可检测的能量的标签 物质，将神经细胞植入动物的脑中; 并检测标签的能量发射。 在第一实施方案中，生物化学物质是神经递质，标签是荧光团，检测步骤包括在动物颅骨中形成开口，并使用双光子激光扫描显微镜光学检测荧光发射。 可以通过培养表达不同受体的神经细胞同时检测多种生物化学物质，并具有不同的荧光标记，其在可区分的波长处产生荧光信号。

    Temporal sequences with neural networks
    8.
    发明授权
    Temporal sequences with neural networks 失效
    具有神经网络的时间序列

    公开(公告)号：US4752906A

    公开(公告)日：1988-06-21

    申请号：US942431

    申请日：1986-12-16

    申请人： David Kleinfeld

    发明人： David Kleinfeld

    IPC分类号： G06N3/04 ,  G06F15/46

    CPC分类号： G06N3/049

    摘要： A sequence generator employing a neural network having its output coupled to at least one plurality of delay elements. The delayed outputs are fed back to an input interconnection network, wherein they contribute to the next state transition through an appropriate combination of interconnections.

    摘要翻译： 一种采用神经网络的序列发生器，其神经网络具有耦合到至少一个多个延迟元件的输出。 延迟的输出被反馈到输入互连网络，其中它们通过适当的互连组合有助于下一状态转换。