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    Methods for transforming streptomyces and other actinomycetes
    5.
    发明授权
    Methods for transforming streptomyces and other actinomycetes 失效
    转化链霉菌和其他放线菌的方法

    公开(公告)号：US5393665A

    公开(公告)日：1995-02-28

    申请号：US833596

    申请日：1992-02-10

    申请人： Jeffrey T. Fayerman ,  Richard K. Stanzak

    发明人： Jeffrey T. Fayerman ,  Richard K. Stanzak

    IPC分类号： C12N1/21 ,  C12N15/76 ,  C12R1/19 ,  C12R1/465 ,  C12N15/64

    CPC分类号： C12N15/76

    摘要： Novel vectors and methods for a single-stranded DNA mediated gene transfer system via transformation, fusion or transduction of Streptomyces, other actinomycetes, and E. coli using a variety of vectors. Phasmid shuttle vectors of the invention are particularly useful as single-stranded vectors that appear to bypass one or more host cell restriction systems, and thus increase the efficiency of gene transfer into highly restrictive host cell systems. New and useful vectors are provided that allow for the cloning of genes both for increasing the yields of known antibiotics and also for producing new antibiotics, antibiotic derivatives, or any other useful gene product, including a variety of mammalian protein products.

    摘要翻译： 通过使用多种载体转化，融合或转导链霉菌，其他放线菌和大肠杆菌的单链DNA介导的基因转移系统的新型载体和方法。 本发明的噬粒穿梭载体特别可用作看起来绕过一个或多个宿主细胞限制系统的单链载体，从而提高基因转移到高度限制性的宿主细胞系统中的效率。 提供了新的和有用的载体，其允许克隆基因以增加已知抗生素的产量，并且还用于生产新的抗生素，抗生素衍生物或任何其它有用的基因产物，包括多种哺乳动物蛋白质产物。

    Method for using an homologous bacillus promoter and associated natural or modified ribosome binding site-containing DNA sequence in streptomyces
    10.
    发明授权
    Method for using an homologous bacillus promoter and associated natural or modified ribosome binding site-containing DNA sequence in streptomyces 失效
    在链霉菌中使用同源杆菌启动子和相关天然或修饰的核糖体结合位点的DNA序列的方法

    公开(公告)号：US4559300A

    公开(公告)日：1985-12-17

    申请号：US458803

    申请日：1983-01-18

    申请人： Steven Kovacevic ,  Jeffrey T. Fayerman ,  James R. Miller ,  Mark A. Richardson

    发明人： Steven Kovacevic ,  Jeffrey T. Fayerman ,  James R. Miller ,  Mark A. Richardson

    IPC分类号： C12N15/09 ,  C12N1/00 ,  C12N15/74 ,  C12N15/76 ,  C12P21/00 ,  C12N1/20 ,  C12N15/00

    CPC分类号： C12N15/74 ,  C12N15/76 ,  Y10S435/886

    摘要： A method for expressing a functional polypeptide in Streptomyces comprises transforming a Streptomyces host cell with a recombinant DNA expression vector and then culturing the transformed cell under conditions suitable for cell growth. The recombinant DNA expression vector comprises the veg or any other homologous Bacillus promoter, a naturally occurring or modified ribosome binding site-containing DNA sequence and a gene that codes for a functional polypeptide such as human pre-proinsulin. The method is specifically exemplified by use of expression plasmids pOW529, pOW539 and transformants, Streptomyces ambofaciens/pOW529 and Streptomyces ambofaciens/pOW539. The method is broadly applicable and is particularly useful in economically important Streptomyces taxa.

    摘要翻译： 在链霉菌中表达功能性多肽的方法包括用重组DNA表达载体转化链霉菌宿主细胞，然后在适合于细胞生长的条件下培养转化的细胞。 重组DNA表达载体包含veg或任何其他同源芽孢杆菌启动子，天然存在或修饰的含核糖体结合位点的DNA序列和编码诸如人前胰岛素原的功能性多肽的基因。 该方法通过使用表达质粒pOW529，pOW539和转化体，淀粉链霉菌（Streptomyces ambofaciens）/ pOW529和淀粉链霉菌（Stubtomyces ambofaciens）/ pOW539来具体举例说明。 该方法广泛适用，在经济上重要的链霉菌属分类群中特别有用。