公开(公告)号：US20100235951A1

公开(公告)日：2010-09-16

申请号：US12293772

申请日：2007-03-16

申请人： Jeroen Van Rie ,  Frank Meulewaeter ,  Gerben Van Eldik

发明人： Jeroen Van Rie ,  Frank Meulewaeter ,  Gerben Van Eldik

IPC分类号： A01H5/00 ,  C07H21/04 ,  C12N5/10 ,  C12N1/21 ,  A01C7/00 ,  A01C14/00

CPC分类号： C12N15/8286 ,  A01N37/18 ,  C07K14/325 ,  C12N9/0006 ,  C12Y101/0104 ,  Y02A40/162

摘要： The present invention relates to novel gene sequences encoding insecticidal proteins produced by Bacillus thuringiensis strains. Particularly, new chimeric genes encoding a Cry1C, Cry1B or Cry1D protein are provided which are useful to protect plants from insect damage. Also included herein are plant cells or plants comprising such genes and methods of making or using them, as well as plant cells or plants comprising one of such chimeric gene and at least one other of such chimeric genes.

摘要翻译： 本发明涉及编码苏云金芽孢杆菌菌株产生的杀虫蛋白的新基因序列。 特别地，提供了编码Cry1C，Cry1B或Cry1D蛋白的新的嵌合基因，其可用于保护植物免受昆虫损害。 本文还包括植物细胞或植物，其包含这些基因及其制备或使用方法，以及植物细胞或植物，其包含这种嵌合基因之一和至少一种其它嵌合基因。

公开(公告)号：US07148400B1

公开(公告)日：2006-12-12

申请号：US09551494

申请日：2000-04-18

申请人： Frank Meulewaeter ,  Marc Cornelissen ,  John Jacobs ,  Gerben Van Eldik ,  Michael Metzlaff

发明人： Frank Meulewaeter ,  Marc Cornelissen ,  John Jacobs ,  Gerben Van Eldik ,  Michael Metzlaff

IPC分类号： C12N15/82 ,  C12N15/83 ,  C12N15/09

CPC分类号： C12N15/8203 ,  C12N15/8218

摘要： The invention provides methods and means for the identification of genes involved in the determination of a plant trait or for the identification encoded by a nucleic acid comprising a determined nucleotide sequence. The invention also provides kits comprising viral RNA vectors derived from satellite viruses and corresponding helper viruses for the introduction of inhibitory RNA into plant cells and plants.

摘要翻译： 本发明提供了用于鉴定涉及植物性状的确定或由包含确定的核苷酸序列的核酸编码的鉴定的基因的方法和手段。 本发明还提供了包含衍生自卫星病毒的病毒RNA载体和用于将抑制性RNA引入植物细胞和植物的相应辅助病毒的试剂盒。

公开(公告)号：US07554005B2

公开(公告)日：2009-06-30

申请号：US11715375

申请日：2007-03-08

申请人： Greet Vanderkimpen ,  Gerben Van Eldik ,  Frank Meulewaeter

发明人： Greet Vanderkimpen ,  Gerben Van Eldik ,  Frank Meulewaeter

IPC分类号： C12N15/63 ,  C12N15/82 ,  C12N5/14

CPC分类号： C12N15/8286 ,  C07K14/415 ,  C12N15/8227 ,  Y02A40/162

摘要： The invention relates to the isolation of promoters from corn capable of directing transcription of an operably linked foreign DNA sequence preferentially, selectively or exclusively in the roots of plants, such as corn plants. The invention also relates to the use of chimeric genes for the preferential or selective expression of biologically active RNA of interest in the roots of plants, such as corn plants. Plants, such as corn plants, comprising corn root preferential or selective promoters operably linked to a foreign DNA sequence which, upon transcription, yield biologically active RNA preferentially or selectively in the roots of plants are also provided.

摘要翻译： 本发明涉及从玉米分离启动子，能够优先选择性地或排他地指导植物根系如玉米植物的可操作地连接的外源DNA序列的转录。 本发明还涉及嵌合基因在植物根系如玉米植物中优先或选择性表达感兴趣的生物活性RNA的用途。 还提供了植物，例如玉米植物，其包含与外来DNA序列可操作地连接的玉米根优先或选择性启动子，其在转录时优选或选择性地在植物根中产生生物活性RNA。

公开(公告)号：US20070174938A1

公开(公告)日：2007-07-26

申请号：US11715375

申请日：2007-03-08

申请人： Greet Vanderkimpen ,  Gerben Van Eldik ,  Frank Meulewaeter

发明人： Greet Vanderkimpen ,  Gerben Van Eldik ,  Frank Meulewaeter

IPC分类号： A01H5/00 ,  C07H21/04 ,  C12N5/04 ,  C07K14/415

CPC分类号： C12N15/8286 ,  C07K14/415 ,  C12N15/8227 ,  Y02A40/162

摘要： The invention relates to the isolation of promoters from corn capable of directing transcription of an operably linked foreign DNA sequence preferentially, selectively or exclusively in the roots of plants, such as corn plants. The invention also relates to the use of chimeric genes for the preferential or selective expression of biologically active RNA of interest in the roots of plants, such as corn plants. Plants, such as corn plants, comprising corn root preferential or selective promoters operably linked to a foreign DNA sequence which, upon transcription, yield biologically active RNA preferentially or selectively in the roots of plants are also provided.

公开(公告)号：US07205454B2

公开(公告)日：2007-04-17

申请号：US10623500

申请日：2003-07-22

申请人： Greet Vanderkimpen ,  Gerben Van Eldik ,  Frank Meulewaeter

发明人： Greet Vanderkimpen ,  Gerben Van Eldik ,  Frank Meulewaeter

IPC分类号： C12N15/82 ,  A01H5/10 ,  C07H21/04

CPC分类号： C12N15/8286 ,  C07K14/415 ,  C12N15/8227 ,  Y02A40/162

摘要： The invention relates to the isolation of promoters from corn capable of directing transcription of an operably linked foreign DNA sequence preferentially, selectively or exclusively in the roots of plants, such as corn plants. The invention also relates to the use of chimeric genes for the preferential or selective expression of biologically active RNA of interest in the roots of plants, such as corn plants. Plants, such as corn plants, comprising corn root preferential or selective promoters operably linked to a foreign DNA sequence which, upon transcription, yield biologically active RNA preferentially or selectively in the roots of plants are also provided.

摘要翻译： 本发明涉及从玉米分离启动子，能够优先选择性地或排他地指导植物根系如玉米植物的可操作地连接的外源DNA序列的转录。 本发明还涉及嵌合基因在植物根系如玉米植物中优先或选择性表达感兴趣的生物活性RNA的用途。 还提供了植物，例如玉米植物，其包含与外来DNA序列可操作地连接的玉米根优先或选择性启动子，其在转录时优选或选择性地在植物根中产生生物活性RNA。

公开(公告)号：US08933297B2

公开(公告)日：2015-01-13

申请号：US13376912

申请日：2010-06-10

申请人： Koen Weterings ,  Gerben Van Eldik

发明人： Koen Weterings ,  Gerben Van Eldik

IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/01 ,  C12N15/113 ,  C12N15/63 ,  C12N15/82 ,  C12P21/00 ,  C12N5/04 ,  C12N5/10 ,  C12N15/54 ,  C12N9/10

CPC分类号： C12N9/1077 ,  C12N15/8257 ,  C12N15/8258

摘要： The invention provides Nicotiana benthamiana mutant plants which are incapable of forming xylosyl-structures on glycoproteins. In addition, the invention provides methods for the production of heterologous glycoproteins in said mutant plants.

摘要翻译： 本发明提供了不能在糖蛋白上形成木糖基结构的烟草本生烟草突变体植物。 此外，本发明提供了在所述突变体植物中产生异源糖蛋白的方法。

公开(公告)号：US20100154081A1

公开(公告)日：2010-06-17

申请号：US12601131

申请日：2008-05-20

申请人： Koen Weterings ,  Gerben Van Eldik ,  Bicke Nagels

发明人： Koen Weterings ,  Gerben Van Eldik ,  Bicke Nagels

IPC分类号： A01H5/00 ,  C12P21/00 ,  C12N5/04

CPC分类号： C12N15/8257 ,  C12N15/8245

摘要： The invention provides methods to modify the N-glycosylation pattern of glycoproteins in higher plant cells, through reducing or eliminating the level of β(1,2) xylosyltransferase and α (1,3) fucosyltransferase activity and increasing the β(1,4) galactosyltransferase activity in the cell of the higher plant.

摘要翻译： 本发明提供了通过降低或消除（1,2）木糖基转移酶和α（1,3）岩藻糖基转移酶活性的水平来增加高等植物细胞中糖蛋白的N-糖基化模式的方法， 4）高等植物细胞中的半乳糖基转移酶活性。

公开(公告)号：US20120083014A1

公开(公告)日：2012-04-05

申请号：US13376912

申请日：2010-06-10

申请人： Koen Weterings ,  Gerben Van Eldik

发明人： Koen Weterings ,  Gerben Van Eldik

IPC分类号： C12P21/02 ,  C12N5/10 ,  A01H5/10 ,  A01H5/00

CPC分类号： C12N9/1077 ,  C12N15/8257 ,  C12N15/8258

摘要： The invention provides Nicotiana benthamiana mutant plants which are incapable of forming xylosyl-structures on glycoproteins. In addition, the invention provides methods for the production of heterologous glycoproteins in said mutant plants.

摘要翻译： 本发明提供了不能在糖蛋白上形成木糖基结构的烟草本生烟草突变体植物。 此外，本发明提供了在所述突变体植物中产生异源糖蛋白的方法。

公开(公告)号：US20100242128A1

公开(公告)日：2010-09-23

申请号：US12740433

申请日：2007-10-31

申请人： Herta Steinkellner ,  Richard Strasser ,  Josef Glössl ,  Koen Weterings ,  Gerben Van Eldik

发明人： Herta Steinkellner ,  Richard Strasser ,  Josef Glössl ,  Koen Weterings ,  Gerben Van Eldik

IPC分类号： A01H1/02 ,  C12N5/04 ,  C12N5/10 ,  A01H1/00 ,  C12Q1/02 ,  C07K14/00 ,  C12Q1/68 ,  C07H21/04 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N9/1051 ,  C12N15/8246 ,  C12N15/8258

摘要： Provided is a novel method to produce a plant cell or plant having an altered N-glycosylation pattern resulting, in particular, in a low level of β-1,2-xylose residues and core α-1,3-fucose residues on protein-bound N-glycans. The plant cell or plant is of particular interest for producing therapeutic glycoproteins with a low, or not detectable, level of β-1,2-xylose and α-1,3-fucose residues. Also provided are novel α-1,3-fucosyltransferase nucleotide sequences and uses.

摘要翻译： 提供了一种产生具有改变的N-糖基化模式的植物细胞或植物的新方法，特别是在蛋白质上的低水平的1,2-木糖残基和核心的α-1,3-岩藻糖残基 N-聚糖。 用于生产具有低或不可检测水平的1,2-木糖和α-1,3-岩藻糖残基的治疗性糖蛋白特别有意义的植物细胞或植物。 还提供了新的α-1,3-岩藻糖基转移酶核苷酸序列和用途。