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1.发明申请公开(公告)号：US20190177441A1
公开(公告)日：2019-06-13
申请号：US16215414
申请日：2018-12-10
发明人： Jian YIN , Jing HU , Peter Seeberger , Xiaopeng ZOU
IPC分类号： C08B37/00
CPC分类号： C08B37/006 , A61K39/00
摘要： The disclosure discloses a preparation method of outer core octasaccharide of Helicobacter pylori lipopolysaccharide, and belongs to the field of carbohydrate chemistry. The structure of the outer core octasaccharide of Helicobacter pylori is (α-D-Glc-(1-3)-α-D-Glc-(1-4)-β-D-Gal-(1-7)-D-α-D-Hep[α-D-Glc-(1-6)-α-D-Glc-(1-6)-α-D-Glc-(1-2)-D-α-D-Hep]-Linker. The structure consists of three monosaccharides: glucose, galactose and heptose. It contains five α-glycosidic bonds of glucose, one β-glycosidic bond of galactose and two α-glycosidic bonds of heptose. The disclosure prepares the octasaccharide by the synergistic action of remote neighboring group participation effect, solvent effect, temperature effect, additives and leaving groups. The reducing end of the octasaccharide may also be linked with a linker for future attachment to the protein to form a glycoconjugate for immunological studies.
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2.发明申请公开(公告)号：US20190160007A1
公开(公告)日：2019-05-30
申请号：US16215395
申请日：2018-12-10
发明人： Jian YIN , Jing HU , Peter Seeberger , Zhou YE
IPC分类号： A61K9/107 , A61K47/32 , A61K49/00 , A61K47/26 , A61K31/65 , C12N15/113 , A61K31/7088 , A61P35/00
摘要： The present disclosure discloses an integrated nano system for liver-targeting co-delivery of genes/drugs and a preparation method, belonging to the field of biomedicines. In the disclosure, a plurality of functions are integrated in a carrier having good biocompatibility and safety, a nucleic acid/drug-loading copolymer portion having a pH-stimulating response function is formed by poly(N,N-dimethylaminoethyl methacrylate) (PDMAEMA) grafted with poly(3-azido-2-hydroxypropylmethacrylate) (PGMA-N3), and a fluorescence-based imaging component Rhodamine B (RhB) and galactose are used as targeting ligands. The drug delivery system provided by the present disclosure is safe, is capable of taking a synergistic effect of gene/drug therapy, and is expected to play a great role in clinical application.
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3.发明申请公开(公告)号：US20190233459A1
公开(公告)日：2019-08-01
申请号：US16215349
申请日：2018-12-10
发明人： Jian YIN , Jing HU , Peter Seeberger , Chunjun QIN
摘要： The present disclosure discloses the chemical synthesis method of the Plesiomonas shigelloides serotype O51 O-antigen oligosaccharide, belonging to the field of chemistry. Source-abundant D-glucose, L-fucose, D-glucosamine and the like are used as raw materials to prepare three glycosylation building blocks, the synthetic route composed of 11 reaction modules is designed, and through the optimization of protecting group and the optimization of the time of introducing functional group, the preparation of the target oligosaccharide chain is successfully achieved. The oligosaccharide chain prepared in the present disclosure has the advantages of cheap and easy-to-get raw materials, and simple and easy-to-repeat preparation method. The present disclosure will have good application prospects in the aspects of development of new drugs and vaccines of Plesiomonas shigelloides, and the like.
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4.发明申请公开(公告)号：US20210002388A1
公开(公告)日：2021-01-07
申请号：US17004067
申请日：2020-08-27
申请人： Jiangnan University
发明人： Jian YIN , Jing HU , Juntao CAI
摘要： The present disclosure discloses a method for preparation of derivatives of gram-positive bacteria surface capsular polysaccharide, and belongs to the field of carbohydrate chemistry. The present disclosure takes glucose as a glycosyl donor to obtain a target β-glucosidic bond, then successfully synthesizes a disaccharide building block through a method of redox of a glucose C-2 site, and then takes the disaccharide building block as a repeat unit to synthesize a target oligosaccharide structure such as a derivative [→3)-α-D-Manp-(1→4)-β-D-Rhap-(1→]5-Linker of gram-positive bacteria cell wall capsular polysaccharide. A reduction end of decose is linked with a linker to be linked with a protein to make glycoconjugates for immunological studies. The method provided by the present disclosure is simple, time-saving, labor-saving and low-cost, and the resultant derivatives of the gram-positive bacteria surface capsular polysaccharide may be used for development and preparation of medicine related to autism.
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公开(公告)号：US20220315711A1
公开(公告)日：2022-10-06
申请号：US17846269
申请日：2022-06-22
申请人： Jiangnan University
发明人： Jing HU , Jian YIN , Xiang WANG
摘要： The present disclosure discloses a preparation method and use of a crosslinked hydrogel for muscle stem cell culture, and belongs to the technical field of biological food materials. Chitosan, alginate, dextran and Ca2+ are crosslinked through physical crosslinking to form a double-network hydrogel with a high mechanical strength, the hydrogel is coated with heparin and collagen through dip coating, such that the hydrogel can immobilize growth factors and adhere to cells. Meanwhile, extracted primary muscle stem cells are inoculated onto the hydrogel and cultured in a growth medium (79% of DMEM, 10% of FBS and 1% of double antibodies) for 24 h. The cells are cultured in an incubator with a differential medium (97% of DMEM, 2% of horse serum and 1% of double antibodies) for 7 d. The hydrogel can enhance the absorption to nutrient substances by the muscle stem cells and facilitate growth of the muscle stem cells. The double-network hydrogel has the potential to be a scaffold for growth of muscle stem cells for cultured meat from stem cells.
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公开(公告)号：US20220333080A1
公开(公告)日：2022-10-20
申请号：US17846227
申请日：2022-06-22
申请人： Jiangnan University
发明人： Jing HU , Jian YIN , Xiang WANG
IPC分类号： C12N5/077
摘要： The present disclosure discloses a crosslinked hydrogel for muscle stem cell culture and a preparation method and use thereof, and belongs to the technical field of biological food materials. The preparation method includes: dissolving collagen to prepare a solution and adding a certain amount of alginate and heparan sulfate proteoglycan for being uniformly mixed with the collagen solution; and adding ε-PL and TGase into the solution, uniformly stirring, and putting a slurry into a mold for crosslinking to obtain the hydrogel. The hydrogel is prepared by linking the collagen, the polylysine and the heparan sulfate proteoglycan using the TGase to form covalent crosslinking, and forming a compact three-dimensional “egg box” network structure through a physical electrostatic interaction between the polylysine and the alginate. The hydrogel can enhance the absorption to nutrient substances by the muscle stem cells and facilitate the growth of the muscle stem cells. The double-network crosslinked hydrogel has the potential to be a scaffold for the growth of muscle stem cells for cultured meat from stem cells.
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公开(公告)号：US20200331951A1
公开(公告)日：2020-10-22
申请号：US16918104
申请日：2020-07-01
申请人： Jiangnan University
发明人： Jian YIN , Jing HU , Zhonghua LIU , Chunjun QIN
IPC分类号： C07H15/203 , C07H15/18 , C07H1/00
摘要： Disclosed is synthesis of an O-Antigen oligosaccharide compound of Helicobacter pylori serotype O2, belonging to the field of organic synthesis. The disclosure obtains O-antigen disaccharide to tetracosasaccharide of Helicobacter pylori serotype O2 by chemical synthesis. A chemical synthesis method which is quite conducive to production of a glucose-α-glucosidic bond is developed in the disclosure by a protectant strategy, temperature effect, solvent effect, and additive effect. The method is applied in synthesis of an O-antigen oligosaccharide compound of Helicobacter pylori serotype O2 assembled with an amino linking arm. A saccharide conjugate can be prepared from the synthesized O-antigen oligosaccharide compound of Helicobacter pylori serotype O2 assembled with an amino linking arm together with a carrier protein for immunology researches, playing an important role in preventing and treating Helicobacter pylori.
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8.发明公开公开(公告)号：US20240068001A1
公开(公告)日：2024-02-29
申请号：US18373423
申请日：2023-09-27
申请人： Jiangnan University
发明人： Jian YIN , Jing HU , Xiaopeng ZOU
IPC分类号： C12P19/04
CPC分类号： C12P19/04
摘要： The disclosure discloses a method for chemically synthesizing a Helicobacter pylori core lipopolysaccharide oligosaccharide antigen carbohydrate chain, and belongs to the technical field of carbohydrate chemistry. The disclosure uses D-glucose, D-galactose and D-mannose as starting materials, which undergo a series of protection and deprotection reactions to prepare eight monosaccharide blocks. The eight monosaccharide block compounds as shown in formulas 2 to 9 undergo glycosylation reactions under catalysis of corresponding activating reagents, to prepare H. pylori lipopolysaccharide core oligosaccharide antigen fragments as shown in formula 1. The disclosure further combines the synthesized oligosaccharide fragments with a chip to make a carbohydrate chip, screens the optimal antigen fragments using patient serum, or combines the synthesized oligosaccharide fragments with carrier proteins to make glycoconjugates.
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公开(公告)号：US20210308280A1
公开(公告)日：2021-10-07
申请号：US17345582
申请日：2021-06-11
申请人： Jiangnan University
IPC分类号： A61K47/69 , A61K31/704 , A61K31/44 , A61K31/513 , A61K41/00 , A61K47/54 , A61K47/60
摘要： The disclosure discloses a glyco-metal-organic frameworks-based hepatic targeted therapeutic drug and a preparation method thereof, and belongs to the field of biomedicine. The disclosure loads a chemotherapeutic drug onto specific metal-organic frameworks, and modifies targeted molecule galactose on the surface of the materials through amide reaction. The biocompatibility and cytotoxicity of the obtained hepatic targeted therapeutic drug have been carefully evaluated at the cellular level. The hepatic targeted therapeutic drug of the disclosure has good stability and acidic pH triggered drug release property, and can exert the synergistic therapeutic effect of photodynamic therapy and chemotherapy. In addition, in vivo behavioral tracing and therapeutic efficacy are evaluated in mouse models with subcutaneous solid tumor and tumor in situ, and the disclosure is expected to play a huge role in clinical applications.
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公开(公告)号：US20200319174A1
公开(公告)日：2020-10-08
申请号：US16906106
申请日：2020-06-19
申请人： Jiangnan University
IPC分类号： G01N33/543 , G01N33/68 , C07K14/765
摘要： The disclosure discloses a method for detecting a human soluble asialoglycoprotein receptor, and belongs to the field of immunological detection. The disclosure provides an economical, rapid, accurate and highly practical ELISA method for detecting a human sASGPR. Based on the specific recognition between a specific ligand of ASGPR and ASGPR, the method selects galactosylated human serum albumin (GSA) as the specific ligand of ASGPR. GSA is prepared from human serum albumin, and has the advantages of cheap price, easy operation, easy storage and the like, and the limit of detection of the method is suitable for the detection of sASGPR in human serum samples. The method can be carried out in ordinary laboratories without using special, large-scale instruments and equipment. The method has the advantages of high specificity, good stability, simple and convenient operation, low cost and the like, and provides a certain reference value for clinical liver function evaluation.
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