公开(公告)号：US4578361A

公开(公告)日：1986-03-25

申请号：US656957

申请日：1984-10-02

申请人： Joachim Siedel ,  Ulrich Neumann ,  Joachim Ziegenhorn ,  Hans-Georg Batz ,  Helmut Lenz ,  Brigitte Pautz ,  Winfried Albert

发明人： Joachim Siedel ,  Ulrich Neumann ,  Joachim Ziegenhorn ,  Hans-Georg Batz ,  Helmut Lenz ,  Brigitte Pautz ,  Winfried Albert

IPC分类号： G01N33/53 ,  A61K38/00 ,  C07K16/18 ,  G01N33/531 ,  G01N33/543 ,  G01N33/577 ,  G01N33/70 ,  A61K39/00

CPC分类号： C07K16/18 ,  G01N33/531 ,  G01N33/54306 ,  G01N33/577 ,  G01N33/70 ,  A61K38/00

摘要： The present invention provides a creatinine antibody and a process for the preparation thereof, wherein a conjugate of creatinine and a material suitable for antiserum formation, which are connected via an aliphatic or araliphatic carboxylic acid as bridge member, is used as immunogen for antiserum formation.The present invention is also concerned with the use of the above antibody for the immunological determination of creatinine, wherein the antibody is incubated with a creatinine-containing sample solution, reacted with a conjugate of creatinine with a hapten carrier substance, whereby one of the components, antibody and conjugate, is present in the solid phase or in dissolved form and the other component is present in dissolved form and the inhibition of the binding reaction between the antibody and the creatinine conjugate is measured.Furthermore, the present invention provides a reagent for the immunological determination of creatinine, wherein it contains the above antibody, a conjugate of creatinine with a hapten carrier substance and buffer substance.

摘要翻译： 本发明提供一种肌酸酐抗体及其制备方法，其中通过脂肪族或脂环族羧酸作为桥接剂连接肌酸酐和适于抗血清形成的物质的缀合物，用作抗血清形成的免疫原。 本发明还涉及上述抗体用于肌酸酐免疫学测定的用途，其中将抗体与含肌酸酐的样品溶液一起温育，与肌酸酐的缀合物与半抗原载体物质反应，由此其中一种成分 ，抗体和缀合物以固相或溶解形式存在，另一组分以溶解形式存在，并测量抗体与肌酐结合物之间结合反应的抑制。 此外，本发明提供了用于肌酸酐免疫测定的试剂，其中含有上述抗体，肌酸酐与半抗原载体物质和缓冲物质的结合物。

公开(公告)号：US4543325A

公开(公告)日：1985-09-24

申请号：US449925

申请日：1982-12-15

申请人： Winfried Albert ,  Joachim Ziegenhorn ,  Joachim Siedel ,  Hans-Georg Batz ,  Helmut Lenz ,  Brigitte Pautz

发明人： Winfried Albert ,  Joachim Ziegenhorn ,  Joachim Siedel ,  Hans-Georg Batz ,  Helmut Lenz ,  Brigitte Pautz

IPC分类号： G01N33/53 ,  G01N33/70 ,  C12N9/96 ,  C12Q1/34

CPC分类号： G01N33/70 ,  Y10S435/81 ,  Y10S435/961 ,  Y10S436/822

摘要： The present invention provides a process for the immunological determination of creatinine, wherein creatinine is converted into 1-methylhydantoin, the 1-methylhydantoin formed is incubated in an aqueous medium with antibodies which are directed against a conjugate of a first hydantoin derivative of the general formula: ##STR1## in which R.sup.1, R.sup.2, R.sup.3 and R.sup.4, which can be the same or different, are hydrogen atoms, alkyl radicals containing up to 3 carbon atoms or phenyl radicals, with a first hapten carrier substance suitable for antibody formation, reacted with a conjugate of a second hydantoin derivative of general formula (I) with a second hapten carrier substance, one of the components antibody and conjugate being present in the solid phase or in dissolved form and the other component being present in dissolved form, and the inhibition of the binding reaction between the antibodies and the hydantoin conjugate with the second hapten carrier substance is measured.The present invention also provides a reagent for the immunological determination of creatinine, wherein it contains creatinine iminohydrolase, antibodies against a conjugate of a hydantoin of the general formula: ##STR2## in which R.sup.1, R.sup.2, R.sup.3 and R.sup.4, which can be the same or different, are hydrogen atoms, alkyl radicals containing up to 3 carbon atoms or phenyl radicals, with a first hapten carrier substance, a conjugate of a hydantoin of general formula (I) with a second hapten carrier substance which does not cross-react substantially with the first hapten carrier substance and a buffer substance.

摘要翻译： 本发明提供了一种免疫学测定肌酐的方法，其中将肌酐转化为1-甲基乙内酰脲，所形成的1-甲基乙内酰脲在水性介质中与针对通式 ：其中可以相同或不同的R 1，R 2，R 3和R 4为氢原子，含有至多3个碳原子的烷基或苯基，其中第一半抗原载体物质适用于抗体 与通式（I）的第二乙内酰脲衍生物与第二半抗原载体物质的缀合物反应，抗体和缀合物之一是固相或溶解形式存在，另一种成分以溶解形式存在 并且测量了与第二半抗原载体物质的抗体和乙内酰脲缀合物之间的结合反应的抑制。 本发明还提供了一种用于免疫测定肌酐的试剂，其中含有肌酐亚氨基水解酶，针对通式如下的乙内酰脲的缀合物的抗体：其中R1，R2，R3和R4可以相同 或不同的是氢原子，含有至多3个碳原子的烷基或苯基，具有第一半抗原载体物质，通式（I）的乙内酰脲与不会基本上交叉反应的第二半抗原载体物质的缀合物 具有第一半抗原载体物质和缓冲物质。

公开(公告)号：US4485177A

公开(公告)日：1984-11-27

申请号：US412411

申请日：1982-08-27

申请人： Joachim Siedel ,  Ulrich Neumann ,  Joachim Ziegenhorn ,  Hans-George Batz ,  Helmut Lenz ,  Brigitte Pautz ,  Winfried Albert

发明人： Joachim Siedel ,  Ulrich Neumann ,  Joachim Ziegenhorn ,  Hans-George Batz ,  Helmut Lenz ,  Brigitte Pautz ,  Winfried Albert

IPC分类号： G01N33/53 ,  A61K38/00 ,  C07K16/18 ,  G01N33/531 ,  G01N33/543 ,  G01N33/577 ,  G01N33/70 ,  G01N33/54 ,  A61K39/00 ,  C07G7/00

CPC分类号： C07K16/18 ,  G01N33/531 ,  G01N33/54306 ,  G01N33/577 ,  G01N33/70 ,  A61K38/00

摘要： The present invention provides a creatinine antibody and a process for the preparation thereof, wherein a conjugate of creatinine and a material suitable for antiserum formation, which are connected via an aliphatic or araliphatic carboxylic acid as bridge member, is used as immunogen for antiserum formation.The present invention is also concerned with the use of the above antibody for the immunological determination of creatinine, wherein the antibody is incubated with a creatinine-containing sample solution, reacted with a conjugate of creatinine with a hapten carrier substance, whereby one of the components, antibody and conjugate, is present in the solid phase or in dissolved form and the other component is present in dissolved form and the inhibition of the binding reaction between the antibody and the creatinine conjugate is measured.Furthermore, the present invention provides a reagent for the immunological determination of creatinine, wherein it contains the above antibody, a conjugate of creatinine with a hapten carrier substance and buffer substance.

摘要翻译： 本发明提供一种肌酸酐抗体及其制备方法，其中通过脂肪族或脂环族羧酸作为桥接剂连接肌酸酐和适于抗血清形成的物质的缀合物，用作抗血清形成的免疫原。 本发明还涉及上述抗体用于肌酸酐免疫学测定的用途，其中将抗体与含肌酸酐的样品溶液一起温育，与肌酸酐的缀合物与半抗原载体物质反应，由此其中一种成分 ，抗体和缀合物以固相或溶解形式存在，另一组分以溶解形式存在，并测量抗体与肌酐结合物之间结合反应的抑制。 此外，本发明提供了用于肌酸酐免疫测定的试剂，其中含有上述抗体，肌酸酐与半抗原载体物质和缓冲物质的结合物。

公开(公告)号：US4851335A

公开(公告)日：1989-07-25

申请号：US908031

申请日：1986-09-16

申请人： Lorenz Kerscher ,  Joachim Siedel ,  Joachim Ziegenhorn ,  Brigitte Pautz

发明人： Lorenz Kerscher ,  Joachim Siedel ,  Joachim Ziegenhorn ,  Brigitte Pautz

IPC分类号： C12Q1/00 ,  C12Q1/26 ,  C12Q1/44 ,  C12Q1/60 ,  G01N33/92

CPC分类号： C12Q1/60 ,  G01N33/92

摘要： The present invention provides a process for the specific determination of HDL cholesterol in serum or plasma by incubation with a cholesterol detection system, containing cholesterol oxidase and cholesterol esterase in a buffered aqueous medium, and measurement of a product of the cholesterol oxidase reaction or of the oxygen consumption, wherein a sample to be tested is incubated in the presence of a salt of a bile acid or of a bile acid derivative or of dioctylsulphosuccinate, a first measurement is then carried out, subsequently a non-ionic, polyethylene oxide group-containing detergent or a secondary alkane sulphonate is added, again incubated and a second measurement is carried out, the amount of HDL cholesterol being determined from the difference between the first and second measurement.The present invention also provides a reagent for the specific determination of HDL cholesterol in serum or plasma containing cholesterol oxidase, buffer and cholesterol esterase, wherein, referred to the final solution, it contains0.1 to 10 U/ml. cholesterol esterase0.05 to 10 U/ml. cholesterol oxidase20 to 500 mmole/liter buffer substance (pH 6.0 to 8.0)0.2 to 20 mmole/liter of a salt of a bile acid or of a bile acid derivative or of dioctylsulphosuccinate and, separately therefrom,0.02 to 2% non-ionic polyethylene oxide group-containing detergent or secondary alkane sulphonate, and optionally 0.05 to 2% of an alcohol containing up to 3 carbon atoms.

摘要翻译： 本发明提供了通过与在缓冲的水性介质中含有胆固醇氧化酶和胆固醇酯酶的胆固醇检测系统温育并测量胆固醇氧化酶反应或胆固醇氧化酶反应产物的方法来特异性测定血清或血浆中的HDL胆固醇的方法 氧气消耗，其中将待测试样品在胆汁酸或胆汁酸衍生物或二辛基磺基琥珀酸盐的存在下孵育，然后进行第一次测量，随后进行非离子的含聚氧乙烯基团 加入洗涤剂或仲烷基磺酸盐，再次孵育并进行第二次测量，由第一次和第二次测量之间的差异确定HDL胆固醇的量。 本发明还提供了用于特异性测定含有胆固醇氧化酶，缓冲液和胆固醇酯酶的血清或血浆中的HDL胆固醇的试剂，其中称为最终溶液，其含有0.1至10U / ml。 胆固醇酯酶0.05〜10U / ml。 胆固醇氧化酶20-500毫摩尔/升缓冲物质（pH 6.0〜8.0）0.2〜20mmol /升胆汁酸或胆汁酸衍生物或二辛基磺基琥珀酸盐的盐，分别为0.02〜2％的非离子性 含有聚环氧乙烷的洗涤剂或仲烷烃磺酸盐，以及任选地0.05至2％的含有至多3个碳原子的醇。

公开(公告)号：US4892815A

公开(公告)日：1990-01-09

申请号：US107467

申请日：1987-10-06

申请人： Lorenz Kerscher ,  Brigitte Pautz ,  Gisela Trunk ,  Joachim Ziegenhorn

发明人： Lorenz Kerscher ,  Brigitte Pautz ,  Gisela Trunk ,  Joachim Ziegenhorn

IPC分类号： C12Q1/26 ,  C12Q1/44 ,  C12Q1/60 ,  G01N33/92

CPC分类号： C12Q1/60 ,  G01N33/92 ,  G01N2800/044 ,  Y10S435/805 ,  Y10S435/962 ,  Y10S436/81 ,  Y10S436/824

摘要： The present invention provides a process for the specific determination of the cholesterol of the HDL fraction in the presence of the LDL fraction of serum lipoproteins. Pancreatic cholesterol esterase is used to liberate cholesterol, and the liberated cholesterol then reacts with cholesterol oxidase and oxygen to form hydrogen peroxide. The kinetics of either of hydrogen peroxide formation or oxygen consumption is measured within 2 to 15 minutes after the start of the reaction between cholesterol and the oxidase. The temperature is maintained within a range of 20.degree. C., during a predetermined time interval. Specific concentrations of reactants are maintained in the reaction solution, i.e., from 0.05 to 30 U/ml pancreatic cholesterol esterase; from 0.1 to 50 U/ml cholesterol oxidase; from 1.0 to 20 mMole/liter of a tenside of the bile acid group, and 0.1 to 10 g/liter of a non-ionic detergent. The pH is kept within a range of 5 to 9. In addition, a reagent is provided which is used for specific determination of HDL fraction cholesterol in the presence of the LDL fraction of serum lipoproteins. The reagent contains pancreatic esterase (0.05 to 30 U/ml); cholesterol oxidase (0.1 to 50 U/ml) a tenside of the bile acid grop (1.5 to 8 mMole/liter) and a non-ionic detergent (0.1 to 10 g/liter), all concentrations referring to the dilution used in the test. The reagent also contains a buffer at a pH of from 5 to 9, and a system for photometric determination of hydrogen peroxide.

摘要翻译： 本发明提供了在血清脂蛋白的LDL级分存在下具体测定HDL级分的胆固醇的方法。 胰胆固醇酯酶用于释放胆固醇，然后释放的胆固醇与胆固醇氧化酶和氧气反应形成过氧化氢。 在胆固醇和氧化酶反应开始后2至15分钟内测量过氧化氢形成或氧气消耗的动力学。 在预定的时间间隔内将温度保持在20℃的范围内。 反应物的特定浓度保持在反应溶液中，即0.05至30U / ml胰胆固醇酯酶; 0.1至50U / ml胆固醇氧化酶; 1.0至20mMole /升的胆汁酸基的表面活性剂和0.1至10g /升的非离子型洗涤剂。 pH值保持在5〜9的范围。此外，提供了在血清脂蛋白的LDL级分存在下用于特异性测定HDL级分胆固醇的试剂。 试剂含有胰酯酶（0.05〜30U / ml）; 胆固醇氧化酶（0.1至50U / ml）胆汁酸组织（1.5至8mMole /升）的表面活性剂和非离子型洗涤剂（0.1至10g /升），所有浓度均指试验中使用的稀释液 。 该试剂还含有pH为5至9的缓冲液和用于光度测定过氧化氢的系统。