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    Mutant fatty acid desaturase
    2.
    发明授权
    Mutant fatty acid desaturase 有权
    突变型脂肪酸去饱和酶

    公开(公告)号：US06686186B2

    公开(公告)日：2004-02-03

    申请号：US09988929

    申请日：2001-12-03

    申请人： John Shanklin ,  Edgar B. Cahoon

    发明人： John Shanklin ,  Edgar B. Cahoon

    IPC分类号： C12N902

    CPC分类号： C12N9/0083 ,  C12N15/8247

    摘要： The present invention relates to a method for producing mutants of a fatty acid desaturase having a substantially increased activity towards fatty acid substrates with chains containing fewer than 18 carbons relative to an unmutagenized precursor desaturase having an 18 carbon atom chain length substrate specificity. The method involves inducing one or more mutations in the nucleic acid sequence encoding the precursor desaturase, transforming the mutated sequence into an unsaturated fatty acid auxotroph cell such as MH13 E. coli, culturing the cells in the absence of supplemental unsaturated fatty acids, thereby selecting for recipient cells which have received and which express a mutant fatty acid desaturase with an elevated specificity for fatty acid substrates having chain lengths of less than 18 carbon atoms. A variety of mutants having 16 or fewer carbon atom chain length substrate specificities are produced by this method. Mutant desaturases produced by this method can be introduced via expression vectors into prokaryotic and eukaryotic cells and can also be used in the production of transgenic plants which may be used to produce specific fatty acid products.

    摘要翻译： 本发明涉及一种生产脂肪酸去饱和酶突变体的方法，该突变体相对于具有18个碳原子链长基底特异性的未成熟前体去饱和酶，具有对具有少于18个碳的链的脂肪酸底物具有显着增加的活性。 该方法包括在编码前体去饱和酶的核酸序列中诱导一个或多个突变，将突变的序列转化为不饱和脂肪酸营养缺陷型细胞如MH13大肠杆菌，在不存在补充不饱和脂肪酸的情况下培养细胞，从而选择 对于接受并且表达具有链长度小于18个碳原子的脂肪酸底物具有特异性提高的突变脂肪酸去饱和酶的受体细胞。 通过该方法制备具有16个或更少碳原子链长基底特异性的各种突变体。 通过该方法产生的突变型去饱和酶可以通过表达载体导入原核和真核细胞中，并且还可用于生产可用于产生特定脂肪酸产物的转基因植物。

    Modified acyl-ACP desaturase
    4.
    发明授权
    Modified acyl-ACP desaturase 有权
    改性酰基-ACP去饱和酶

    公开(公告)号：US6100091A

    公开(公告)日：2000-08-08

    申请号：US276295

    申请日：1999-03-25

    申请人： Edgar B. Cahoon ,  John Shanklin ,  Ylva Lindgvist ,  Gunter Schneider

    发明人： Edgar B. Cahoon ,  John Shanklin ,  Ylva Lindgvist ,  Gunter Schneider

    IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/21 ,  C12N5/10 ,  C12N9/02 ,  C12N15/00 ,  C12N15/11 ,  C12N15/29 ,  C12N15/82 ,  C07H21/04 ,  C12N5/14

    CPC分类号： C12N9/0083 ,  C12N15/8247 ,  C07K2319/00

    摘要： Disclosed is a methods for modifying the chain length and double bond positional specificities of a soluble plant fatty acid desaturase. More specifically, the method involves modifying amino acid contact residues in the substrate binding channel of the soluble fatty acid desaturase which contact the fatty acid. Specifically disclosed is the modification of an acyl-ACP desaturase. Amino acid contact residues which lie within the substrate binding channel are identified, and subsequently replaced with different residues to effect the modification of activity.

    摘要翻译： 公开了用于改变可溶性植物脂肪酸去饱和酶的链长和双键位置特异性的方法。 更具体地，该方法包括修饰与脂肪酸接触的可溶性脂肪酸去饱和酶的底物结合通道中的氨基酸接触残基。 具体公开的是酰基-ACP去饱和酶的修饰。 鉴定位于底物结合通道内的氨基酸接触残基，随后用不同的残基替换以影响活性的修饰。

    Modified Acyl-ACP desaturase
    5.
    发明授权
    Modified Acyl-ACP desaturase 失效
    改性酰基-ACP去饱和酶

    公开(公告)号：US5888790A

    公开(公告)日：1999-03-30

    申请号：US853979

    申请日：1997-05-09

    申请人： Edgar B. Cahoon ,  John Shanklin ,  Ylva Lindqvist ,  Gunter Schneider

    发明人： Edgar B. Cahoon ,  John Shanklin ,  Ylva Lindqvist ,  Gunter Schneider

    IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/21 ,  C12N5/10 ,  C12N9/02 ,  C12N15/00 ,  C12N15/11 ,  C12N15/29 ,  C12N15/82

    CPC分类号： C12N9/0083 ,  C12N15/8247 ,  C07K2319/00

    摘要： Disclosed is a method for modifying the chain length and double bond positional specificities of a soluble plant fatty acid desaturase. More specifically, the method involves modifying amino acid contact residues in the substrate binding channel of the soluble fatty acid desaturase which contact the fatty acid. Specifically disclosed is the modification of an acyl-ACP desaturase. Amino acid contact residues which lie within the substrate binding channel are identified, and subsequently replaced with different residues to effect the modification of activity.

    摘要翻译： 公开了一种改变可溶性植物脂肪酸去饱和酶的链长和双键位置特异性的方法。 更具体地，该方法包括修饰与脂肪酸接触的可溶性脂肪酸去饱和酶的底物结合通道中的氨基酸接触残基。 具体公开的是酰基-ACP去饱和酶的修饰。 鉴定位于底物结合通道内的氨基酸接触残基，随后用不同的残基替换以影响活性的修饰。

    Methods and compositions relating to plant .DELTA..sup.6 palmitoyl-acyl carrier protein desaturase
    8.
    发明授权
    Methods and compositions relating to plant .DELTA..sup.6 palmitoyl-acyl carrier protein desaturase 失效
    与植物DELTA 6棕榈酰酰基载体蛋白质去饱和酶相关的方法和组合物

    公开(公告)号：US5654402A

    公开(公告)日：1997-08-05

    申请号：US329560

    申请日：1994-10-26

    申请人： Edgar B. Cahoon ,  John B. Ohlrogee

    发明人： Edgar B. Cahoon ,  John B. Ohlrogee

    IPC分类号： C12N9/02 ,  C12N15/82 ,  C07K14/415 ,  C12N9/04 ,  C12N15/29

    CPC分类号： C12N9/0083 ,  C12N15/8247

    摘要： A plant .DELTA..sup.6 palmitoyl-acyl carrier protein desaturase, the gene encoding the desaturase, and transgenic plants and plant cells containing the heterologous DNA encoding the desaturase are described. The desaturase introduces a double bond at the sixth carbon atom from the carboxyl end of a 16 carbon saturated fatty acid, and is therefore useful in production of plant seeds having a modified fatty acid profile.

    摘要翻译： 描述了植物DELTA 6棕榈酰酰基载体蛋白去饱和酶，编码去饱和酶的基因，以及含有编码去饱和酶的异源DNA的转基因植物和植物细胞。 去饱和酶在16碳饱和脂肪酸的羧基末端的第六个碳原子处引入双键，因此可用于生产具有改性脂肪酸分布的植物种子。