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    Nucleic acid labeling methods
    3.
    发明申请
    Nucleic acid labeling methods 有权

    公开(公告)号：US20060160096A1

    公开(公告)日：2006-07-20

    申请号：US10983046

    申请日：2004-11-04

    申请人： Kyle Cole ,  Vivi Truong ,  Glenn McGall ,  Anthony Barone

    发明人： Kyle Cole ,  Vivi Truong ,  Glenn McGall ,  Anthony Barone

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C07H19/20 ,  C07H19/10 ,  C07H21/00

    摘要： In one aspect of the invention, a method is provided for end-labeling RNA (total RNA, mRNA, cRNA or fragmented RNA). In one aspect of the present invention, T4 RNA ligase is used to attach a 3′-labeled AMP or CMP donor to an RNA acceptor molecule. In another embodiment, a pyrophosphate molecule 3′-AppN-3′-linker-detectable moiety is used as donor molecule. In another aspect of the present invention, a method of detecting the presence of an RNA of interest in a sample is provided, the method having the following steps: providing the sample comprising RNA which may or may not have said RNA of interest; treating the sample with a fragmenting reagent to provide RNA fragments; removing phosphate groups from said fragments to provide fragments with free 3′ OH groups; ligating said fragment with a labeling reagent according to the instant invention; providing a nucleic acid array having probes directed to said RNA of interest; hybridizing the labeled nucleic acid fragments to said nucleic acid array; and determining the extent of hybridization to said probes to determine the presence of said RNA of interest.

    Amplification of nucleic acids
    4.
    发明申请
    Amplification of nucleic acids 审中-公开
    核酸扩增

    公开(公告)号：US20050136417A1

    公开(公告)日：2005-06-23

    申请号：US10796323

    申请日：2004-03-09

    申请人： Kyle Cole ,  Vivi Truong ,  Glenn McGall

    发明人： Kyle Cole ,  Vivi Truong ,  Glenn McGall

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6846 ,  C12Q2600/156 ,  C12Q2531/119 ,  C12Q2525/119 ,  C12Q2521/301

    摘要： Methods and kits for amplifying nucleic acids are provided. Double stranded cDNA with uridine residues incorporated into one strand is synthesized and the uridine containing strand is nicked at uridine residues. The DNA is extended from the nicks in the presence of dUTP using a strand displacing enzyme. Repeated cycles of nicking and extension result in amplification of the nucleic acid. Methods are also provided for analysis of the above sample by hybridization to an array, which may be specifically designed to interrogate the collection of target sequences for particular characteristics, such as, for example, the presence or absence of one or more polymorphisms or the presence or absence of a transcript.

    摘要翻译： 提供了用于扩增核酸的方法和试剂盒。 合成具有并入一条链中的尿苷残基的双链cDNA，并将含有尿苷的链在尿苷残基上切口。 使用链置换酶，在dUTP存在下，DNA从缺口延伸。 切口和延伸的重复循环导致核酸的扩增。 还提供了用于通过与阵列杂交来分析上述样品的方法，该阵列可被特别设计用于询问特定特征的靶序列的收集，例如存在或不存在一个或多个多态性或存在 或没有抄本。

    Nucleic Acid Labeling Methods
    6.
    发明申请
    Nucleic Acid Labeling Methods 有权
    核酸标记方法

    公开(公告)号：US20090054258A1

    公开(公告)日：2009-02-26

    申请号：US12256256

    申请日：2008-10-22

    申请人： Kyle B. Cole ,  Vivi Truong ,  Glenn H. McGall ,  Anthony D. Barone

    发明人： Kyle B. Cole ,  Vivi Truong ,  Glenn H. McGall ,  Anthony D. Barone

    IPC分类号： C40B30/04 ,  C07H19/20 ,  C07H19/10

    CPC分类号： C07H19/20 ,  C07H19/10 ,  C07H21/00

    摘要： In one aspect of the invention, a method is provided for end-labeling RNA (total RNA, mRNA, cRNA or fragmented RNA). In one aspect of the present invention, T4 RNA ligase is used to attach a 3′-labeled AMP or CMP donor to an RNA acceptor molecule. In another embodiment, a pyrophosphate molecule 3′-AppN-3′-linker-detectable moiety is used as donor molecule.In another aspect of the present invention, a method of detecting the presence of an RNA of interest in a sample is provided, the method having the following steps: providing the sample comprising RNA which may or may not have said RNA of interest; treating the sample with a fragmenting reagent to provide RNA fragments; removing phosphate groups from said fragments to provide fragments with free 3′ OH groups; ligating said fragment with a labeling reagent according to the instant invention; providing a nucleic acid array having probes directed to said RNA of interest; hybridizing the labeled nucleic acid fragments to said nucleic acid array; and determining the extent of hybridization to said probes to determine the presence of said RNA of interest.

    摘要翻译： 在本发明的一个方面，提供了用于末端标记RNA（总RNA，mRNA，cRNA或片段化RNA）的方法。 在本发明的一个方面，T4 RNA连接酶用于将3'标记的AMP或CMP供体连接到RNA受体分子上。 在另一个实施方案中，将焦磷酸盐分子3'- AppN-3'-接头可检测部分用作供体分子。 在本发明的另一方面，提供了检测样品中目的RNA存在的方法，该方法具有以下步骤：提供包含可能具有或不具有所述目的RNA的RNA的样品; 用碎裂试剂处理样品以提供RNA片段; 从所述片段中除去磷酸基以提供具有游离的3'OH基团的片段; 将所述片段与根据本发明的标记试剂连接; 提供具有针对所述感兴趣的RNA的探针的核酸阵列; 将标记的核酸片段与所述核酸阵列杂交; 以及确定与所述探针的杂交程度，以确定所述目的RNA的存在。

    Downstream genes of tumor suppressor WT1
    7.
    发明授权
    Downstream genes of tumor suppressor WT1 失效
    肿瘤抑制因子WT1的下游基因

    公开(公告)号：US06177248B1

    公开(公告)日：2001-01-23

    申请号：US09256301

    申请日：1999-02-24

    申请人： Jonathan Oliner ,  Vivi Truong ,  Daniel Haber ,  Sean Lee

    发明人： Jonathan Oliner ,  Vivi Truong ,  Daniel Haber ,  Sean Lee

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6827 ,  C12Q2600/158 ,  Y02A90/26

    摘要： Methods are provided for diagnosing cancers, drug-screening, and functionally analyzing mutations involving the WT1 gene. The methods involve use of the newly identified set of genes which are regulated by WT1 as well as by the set of genes which are regulated by WT1 fusions to EWS. Monitoring expression levels of these sets of genes can be used as an indicator of the genetic status of the gene. It can also identify which have similar effects on down-stream genes.

    摘要翻译： 提供了诊断癌症，药物筛选和功能分析涉及WT1基因的突变的方法。 这些方法涉及使用由WT1调节的新鉴定的一组基因以及由WT1融合到EWS调节的一组基因。 监测这些基因组的表达水平可用作基因遗传状态的指标。 它还可以确定哪些对下游基因具有相似的影响。

    Nucleic acid labeling methods
    8.
    发明授权
    Nucleic acid labeling methods 有权
    核酸标记方法

    公开(公告)号：US07824863B2

    公开(公告)日：2010-11-02

    申请号：US12256256

    申请日：2008-10-22

    申请人： Kyle B. Cole ,  Vivi Truong ,  Glenn H. McGall ,  Anthony D. Barone

    发明人： Kyle B. Cole ,  Vivi Truong ,  Glenn H. McGall ,  Anthony D. Barone

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H15/00 ,  C07H21/00 ,  C07H19/04

    CPC分类号： C07H19/20 ,  C07H19/10 ,  C07H21/00

    摘要： In one aspect of the invention, a method is provided for end-labeling RNA (total RNA, mRNA, cRNA or fragmented RNA). In one aspect of the present invention, T4 RNA ligase is used to attach a 3′-labeled AMP or CMP donor to an RNA acceptor molecule. In another embodiment, a pyrophosphate molecule 3′-AppN-3′-linker-detectable moiety is used as donor molecule.In another aspect of the present invention, a method of detecting the presence of an RNA of interest in a sample is provided, the method having the following steps: providing the sample comprising RNA which may or may not have said RNA of interest; treating the sample with a fragmenting reagent to provide RNA fragments; removing phosphate groups from said fragments to provide fragments with free 3′ OH groups; ligating said fragment with a labeling reagent according to the instant invention; providing a nucleic acid array having probes directed to said RNA of interest; hybridizing the labeled nucleic acid fragments to said nucleic acid array; and determining the extent of hybridization to said probes to determine the presence of said RNA of interest.

    摘要翻译： 在本发明的一个方面，提供了用于末端标记RNA（总RNA，mRNA，cRNA或片段化RNA）的方法。 在本发明的一个方面，T4 RNA连接酶用于将3'标记的AMP或CMP供体连接到RNA受体分子上。 在另一个实施方案中，将焦磷酸盐分子3'- AppN-3'-接头可检测部分用作供体分子。 在本发明的另一方面，提供了检测样品中目的RNA存在的方法，该方法具有以下步骤：提供包含可能具有或不具有所述目的RNA的RNA的样品; 用碎裂试剂处理样品以提供RNA片段; 从所述片段中除去磷酸基以提供具有游离的3'OH基团的片段; 将所述片段与根据本发明的标记试剂连接; 提供具有针对所述感兴趣的RNA的探针的核酸阵列; 将标记的核酸片段与所述核酸阵列杂交; 以及确定与所述探针的杂交程度，以确定所述目的RNA的存在。

    Nucleic acid labeling methods
    9.
    发明授权
    Nucleic acid labeling methods 有权
    核酸标记方法

    公开(公告)号：US07504215B2

    公开(公告)日：2009-03-17

    申请号：US10983046

    申请日：2004-11-04

    申请人： Kyle B. Cole ,  Vivi Truong ,  Glenn H. McGall ,  Anthony D. Barone

    发明人： Kyle B. Cole ,  Vivi Truong ,  Glenn H. McGall ,  Anthony D. Barone

    IPC分类号： C12Q1/68 ,  C07H19/04 ,  C07H21/02 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C07H19/20 ,  C07H19/10 ,  C07H21/00

    摘要： In one aspect of the invention, a method is provided for end-labeling RNA (total RNA, mRNA, cRNA or fragmented RNA). In one aspect of the present invention, T4 RNA ligase is used to attach a 3′-labeled AMP or CMP donor to an RNA acceptor molecule. In another embodiment, a pyrophosphate molecule 3′-AppN-3′-linker-detectable moiety is used as donor molecule.In another aspect of the present invention, a method of detecting the presence of an RNA of interest in a sample is provided, the method having the following steps: providing the sample comprising RNA which may or may not have said RNA of interest; treating the sample with a fragmenting reagent to provide RNA fragments; removing phosphate groups from said fragments to provide fragments with free 3′ OH groups; ligating said fragment with a labeling reagent according to the instant invention; providing a nucleic acid array having probes directed to said RNA of interest; hybridizing the labeled nucleic acid fragments to said nucleic acid array; and determining the extent of hybridization to said probes to determine the presence of said RNA of interest.

    摘要翻译： 在本发明的一个方面，提供了用于末端标记RNA（总RNA，mRNA，cRNA或片段化RNA）的方法。 在本发明的一个方面，T4 RNA连接酶用于将3'标记的AMP或CMP供体连接到RNA受体分子上。 在另一个实施方案中，将焦磷酸盐分子3'- AppN-3'-接头可检测部分用作供体分子。 在本发明的另一方面，提供了检测样品中目的RNA存在的方法，该方法具有以下步骤：提供包含可能具有或不具有所述目的RNA的RNA的样品; 用碎裂试剂处理样品以提供RNA片段; 从所述片段中除去磷酸基以提供具有游离的3'OH基团的片段; 将所述片段与根据本发明的标记试剂连接; 提供具有针对所述感兴趣的RNA的探针的核酸阵列; 将标记的核酸片段与所述核酸阵列杂交; 以及确定与所述探针的杂交程度，以确定所述目的RNA的存在。