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1.发明申请公开(公告)号：US20100115661A1
公开(公告)日：2010-05-06
申请号：US12581331
申请日：2009-10-19
申请人： John H. Duesing , Anthony J. Kinney , Daniel Siehl
发明人： John H. Duesing , Anthony J. Kinney , Daniel Siehl
CPC分类号： C12N15/8243 , C12N15/8251 , C12N15/8253 , C12N15/8254
摘要： Compositions and methods for increasing the level of one or more selected free ε and/or α-N-acetylated amino acids in a selected tissue or organ of a plant are provided. In specific embodiments, the plant, plant part, seed or grain comprises a free α-N-acetylated amino acid content of at least 200 ppm or a ratio of free α-N-acetylated amino acid content to free non-α-N-acetylated amino acid content of about 3 to about 1000. Compositions comprising plants, plant parts, seed and grain having stably incorporated into their genome a heterologous polynucleotide encoding an amino acid-N-acetyltransferase polypeptide operably linked to a promoter active in the seed are provided. Further provided are compositions comprising a plant, plant part, seed or grain having stably incorporated into their genome a first heterologous polynucleotide encoding a first amino acid-N-acetyltransferase polypeptide operably linked to a first promoter active in the seed and a second heterologous polynucleotide encoding a second amino acid-N-acetyltransferase polypeptide operably linked to a second promoter active in the seed, wherein the first and the second amino acid-N-acetyltransferase polypeptide acetylate the α-amine of distinct amino acids. Compositions comprising food sources, feed and supplements, along with methods of increasing the nutritional value of a plant, plant part, seed or grain, are further provided.
摘要翻译： 用于增加一个或多个所选择的免费等级的组合和方法 和/或α-N-乙酰化氨基酸在植物的选定组织或器官中。 在具体实施方案中，植物，植物部分，种子或颗粒包含至少200ppm的游离α-N-乙酰化氨基酸含量或游离的α-N-乙酰化氨基酸含量与游离的非α-N- 提供约3至约1000的乙酰化氨基酸含量。提供包含植物，植物部分，种子和颗粒的组合物，其稳定地并入其基因组中，编码与种子中活性物质活化的启动子可操作地连接的氨基酸-N-乙酰转移酶多肽的异源多核苷酸 。 进一步提供的是包含植物，植物部分，种子或谷物的组合物，其稳定地并入其基因组中，第一异源多核苷酸编码与种子中有活性的第一启动子可操作地连接的第一氨基酸-N-乙酰转移酶多肽和第二异源多核苷酸编码 可操作地连接到在种子中有活性的第二启动子的第二氨基酸-N-乙酰转移酶多肽，其中所述第一和第二氨基酸-N-乙酰转移酶多肽对不同氨基酸的α-胺进行乙酰化。 还提供了包含食物来源，饲料和补充剂的组合物，以及增加植物，植物部分，种子或谷物的营养价值的方法。
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公开(公告)号：US20110268861A1
公开(公告)日：2011-11-03
申请号：US13182876
申请日：2011-07-14
IPC分类号： C08G63/48 , A23D9/00 , A23L1/31 , A23C20/00 , A23D7/00 , A23K1/00 , A23L2/52 , A23C9/20 , A23L1/30 , C07C53/126 , A23C9/152
CPC分类号： C12N15/8247 , A23D9/00 , A23K10/30 , A23K20/163 , A23K50/80 , A23L33/12 , C12N9/0083 , C12N9/1029 , Y02A40/818
摘要： Oilseed plants which have been transformed to produce arachidonic acid, recombinant constructs used in such transformations, methods for producing arachidonic acid in a plant are described and uses of oils and seeds obtained from such transformed plants in a variety of food and feed applications are described.
摘要翻译： 已经转化生产花生四烯酸的油籽植物，用于这种转化的重组构建体，植物中花生四烯酸的生产方法，并描述了在各种食品和饲料应用中从这些转化植物获得的油和种子的用途。
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公开(公告)号：US20090138992A1
公开(公告)日：2009-05-28
申请号：US12354018
申请日：2009-01-15
IPC分类号： A01H5/00
CPC分类号： C07H21/04 , C12N9/0083 , C12N9/1029 , C12N15/8247
摘要： This invention relates to an isolated nucleic acid fragment encoding an enzyme involved in lipid biosynthesis. The invention also relates to the construction of a chimeric gene encoding all or a portion of the enzyme involved in lipid biosynthesis, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the enzyme involved in lipid biosynthesis in a transformed host cell.
摘要翻译： 本发明涉及编码参与脂质生物合成的酶的分离的核酸片段。 本发明还涉及在正义或反义方向上编码参与脂质生物合成的全部或部分酶的嵌合基因的构建，其中嵌合基因的表达导致生产参与脂质生物合成的酶水平的变化 转化的宿主细胞。
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公开(公告)号：US07456014B2
公开(公告)日：2008-11-25
申请号：US11476510
申请日：2006-06-28
申请人： Anthony J. Kinney , Kevin L. Stecca
发明人： Anthony J. Kinney , Kevin L. Stecca
CPC分类号： C12N15/8216 , C12N15/8218 , C12N15/8245 , C12N15/8246 , C12N15/8247 , C12N15/825 , C12N15/8251
摘要： Recombinant constructs useful for reducing the expression of endogenous mRNA and any substantially similar endogenous mRNA are disclosed. In particular, a recombinant construct comprising, inter alia, a suitable nucleic acid sequence and its reverse complement can be used to alter the expression of any homologous, endogenous RNA (i.e., the target RNA) which is in proximity to this suitable nucleic acid sequence.
摘要翻译： 公开了可用于减少内源性mRNA的表达和任何基本上相似的内源mRNA的重组构建体。 特别地，包含尤其是合适的核酸序列及其反向互补序列的重组构建体可用于改变任何同源的内源RNA（即靶RNA）的表达，其接近该合适的核酸序列 。
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公开(公告)号：US20080209596A1
公开(公告)日：2008-08-28
申请号：US11970805
申请日：2008-01-08
CPC分类号： C07K14/415 , C12N15/8245
摘要： This invention relates to an isolated nucleic acid fragment encoding a sugar transport protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the sugar transport protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the sugar transport protein in a transformed host cell.
摘要翻译： 本发明涉及编码糖转运蛋白的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码全部或部分糖转运蛋白的嵌合基因，其中嵌合基因的表达导致转化的宿主细胞中糖转运蛋白的水平的改变 。
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公开(公告)号：US07365242B2
公开(公告)日：2008-04-29
申请号：US10757155
申请日：2004-01-14
申请人： Anthony J. Kinney , Gary M. Fader
发明人： Anthony J. Kinney , Gary M. Fader
CPC分类号： C12N15/8216 , C07K14/415 , C12N15/8218 , C12N15/8247 , C12N15/8251
摘要： This invention concerns the construction of transgenic soybean lines wherein the expression of genes encoding seed storage proteins are modulated to effect a change in seed storage protein profile of transgenic plants. Modification of the seed storage protein profile can result in the production of novel soy protein products with unique and valuable functional characteristics.
摘要翻译： 本发明涉及转基因大豆品系的构建，其中编码种子储存蛋白的基因的表达被调节以影响转基因植物的种子储存蛋白谱的变化。 种子储存蛋白质谱的修饰可以产生具有独特且有价值的功能特征的新型大豆蛋白产品。
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公开(公告)号：US07157621B2
公开(公告)日：2007-01-02
申请号：US10183687
申请日：2002-06-27
申请人： Stephen M. Allen , William B. Allen , Rebecca E. Cahoon , Sabine Epelbaum , Omolayo O. Famodu , Leslie T. Harvell , Todd J. Jones , Anthony J. Kinney , Theodore M. Klein , Changjiang Li , Igor Cunha Oliveira , Hajime Sakai , Bo Shen , Mitchell C. Tarczynski
发明人： Stephen M. Allen , William B. Allen , Rebecca E. Cahoon , Sabine Epelbaum , Omolayo O. Famodu , Leslie T. Harvell , Todd J. Jones , Anthony J. Kinney , Theodore M. Klein , Changjiang Li , Igor Cunha Oliveira , Hajime Sakai , Bo Shen , Mitchell C. Tarczynski
CPC分类号： C12N15/8247 , C07K14/415
摘要： The preparation and use of nucleic acid fragments useful in altering the oil phenotype in plants are disclosed. Chimeric construct incorporating such nucleic acid fragments and suitable regulatory sequences can be used to create transgenic plants having altered lipid profiles. Methods for altering the oil phenotype in plants using such nucleic acid fragments also are disclosed.
摘要翻译： 公开了可用于改变植物油表型的核酸片段的制备和使用。 掺入此类核酸片段和合适调节序列的嵌合构建体可用于产生具有改变的脂质分布的转基因植物。 还公开了使用这种核酸片段改变植物油表型的方法。
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公开(公告)号：US06900369B2
公开(公告)日：2005-05-31
申请号：US10233926
申请日：2002-09-03
申请人： Stephen M. Allen , Karlene H. Butler , Saverio Carl Falco , Anthony J. Kinney , Kevin L. Stecca
发明人： Stephen M. Allen , Karlene H. Butler , Saverio Carl Falco , Anthony J. Kinney , Kevin L. Stecca
CPC分类号： C12N9/1241 , C12N15/8243
摘要： This invention relates to an isolated nucleic acid fragment encoding a cholinephosphate cytidylyltransferase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the cholinephosphate cytidylyltransferase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the cholinephosphate cytidylyltransferase in a transformed host cell.
摘要翻译： 本发明涉及编码胆碱磷酸胞苷酰转移酶的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码全部或部分胆碱磷酸胞苷酰基转移酶的嵌合基因，其中嵌合基因的表达导致转化宿主细胞中胆碱磷酸胞苷酰转移酶水平的改变。
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公开(公告)号：US06864077B1
公开(公告)日：2005-03-08
申请号：US09857524
申请日：1999-12-02
CPC分类号： C12N9/0083
摘要： This invention relates to an isolated nucleic acid fragment encoding a delta-6 desaturase or sphingolipid desaturase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the delta-6 desaturase or sphingolipid desaturase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the delta-6 desaturase or sphingolipid desaturase in a transformed host cell.
摘要翻译： 本发明涉及编码δ-6去饱和酶或鞘脂脱氢酶的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码Δ-6去饱和酶或鞘脂脱氢酶的全部或部分的嵌合基因，其中嵌合基因的表达导致产生改变的δ-6去饱和酶水平 或鞘脂脱氢酶在转化的宿主细胞中。
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公开(公告)号：US06849783B2
公开(公告)日：2005-02-01
申请号：US09740288
申请日：2000-12-19
CPC分类号： C12N15/52
摘要： This invention relates to an isolated nucleic acid fragment encoding a biotin synthases. The invention also relates to the construction of a chimeric gene encoding all or a portion of the biotin synthases, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the biotin synthases in a transformed host cell.
摘要翻译： 本发明涉及编码生物素合酶的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码全部或一部分生物素合酶的嵌合基因，其中嵌合基因的表达导致转化宿主细胞中生物素合酶水平的改变。
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