公开(公告)号：US5798273A

公开(公告)日：1998-08-25

申请号：US719221

申请日：1996-09-25

申请人： John K. Shuler ,  Stephen J. Lovell ,  Abigail S. Fisher ,  Alan J. Weiss ,  Robert W. Rosenstein

发明人： John K. Shuler ,  Stephen J. Lovell ,  Abigail S. Fisher ,  Alan J. Weiss ,  Robert W. Rosenstein

IPC分类号： G01N33/543 ,  G01N33/558 ,  G01N33/563 ,  G01N33/564 ,  G01N33/566

CPC分类号： G01N33/558

摘要： The present invention relates to a method and assay device for detecting small analytes. The results of the assay can be directly read from the device, which is a lateral flow device.

摘要翻译： 本发明涉及用于检测小分析物的方法和测定装置。 可以从作为侧流装置的装置直接读取测定结果。

公开(公告)号：US5776710A

公开(公告)日：1998-07-07

申请号：US771507

申请日：1996-12-23

申请人： Robert A. Levine ,  Stephen C. Wardlaw ,  Leon W. M. M. Terstappen ,  Kristen L. Manion ,  Rodolfo R. Rodriguez ,  Adrien P. Malick ,  Subhash Dhanesar ,  Stephen J. Lovell ,  Alvydas J. Ozinskas

发明人： Robert A. Levine ,  Stephen C. Wardlaw ,  Leon W. M. M. Terstappen ,  Kristen L. Manion ,  Rodolfo R. Rodriguez ,  Adrien P. Malick ,  Subhash Dhanesar ,  Stephen J. Lovell ,  Alvydas J. Ozinskas

IPC分类号： G01N33/543 ,  B01L3/14 ,  G01N33/49 ,  G01N33/537 ,  G01N33/569 ,  G01N33/58 ,  G01N33/558

CPC分类号： B01L3/5021 ,  G01N33/491 ,  G01N33/5375 ,  G01N33/56972 ,  G01N33/585 ,  Y10S435/81 ,  Y10S435/967 ,  Y10S435/971 ,  Y10S435/973 ,  Y10S436/805 ,  Y10S436/81 ,  Y10S436/824 ,  Y10S436/829 ,  Y10T436/111666

摘要： A patient's health may be diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more bodies or groups of bodies such as floats, inserts, liposomes, or plastic beads of different densities. Each density-defined body carries analyte-capture binding materials such as antigens or antibodies, which are specific to an epitope, or other specific high affinity binding site on a target analyte which target analyte may be in the blood or other sample being tested; and the level of which analyte is indicative of the patient's health. At least one labeled binding material which is also specific to an epitope, or other specific high affinity binding site on the target analyte is added to the sample so as to form labeled binding material/analyte/body complexes in the sample. Upon centrifugation, the complexes will settle out in different areas in the tube according to the respective density of the body or bodies; and the degree of label emission of the complex layers can enable qualitative and/or quantitative analyses of the sample to be made. Unbound labeled binding materials will be separated from the complexed layers by the washing action of ascending or descending components of the sample during the centrifugation step. Unbound labeled binding material will thus not interfere with the analysis.

摘要翻译： 可以通过将透明管中的血液样品离心来诊断患者的健康，该管包含一个或多个不同密度的浮体，插入物，脂质体或塑料珠的主体或组。 每个密度定义的身体携带分析物 - 捕获结合材料，例如抗原或抗体，其对靶分析物是特异性的，或靶分析物上的其它特异性高亲和力结合位点，其目标分析物可能在待测试的血液或其他样品中; 并且其分析物的水平表示患者的健康。 将至少一种对靶分析物上的表位或其他特异性高亲和力结合位点特异性的标记结合物质加入到样品中，以便在样品中形成标记的结合材料/分析物/身体复合物。 离心后，复合物将根据身体或身体的相应密度沉淀在管中的不同区域; 并且复合层的标签发射程度可以使得要进行样品的定性和/或定量分析。 通过在离心步骤期间样品的上升或下降组分的洗涤作用，未结合的标记结合材料将从复合层分离。 因此，未结合的标签结合材料不会影响分析。

公开(公告)号：US07776522B2

公开(公告)日：2010-08-17

申请号：US12108550

申请日：2008-04-24

申请人： Richard Ivey ,  Stephen J. Lovell ,  Robert Rosenstein ,  Thomas Gentle ,  Song Shi

发明人： Richard Ivey ,  Stephen J. Lovell ,  Robert Rosenstein ,  Thomas Gentle ,  Song Shi

IPC分类号： C12Q1/68

CPC分类号： G01N33/56983 ,  G01N2333/025

摘要： Methods for diagnosis of HPV infection in a subject are provided. HPV infection in a subject can be determined by generating mass profile data for a biological sample from the subject and correlating the mass profile data with reference mass profiles to detect the presence or absence, and/or quantity of at least one biomarker associated with HPV infection. Methods for detecting at least one biomarker associated with HPV infection in a biological sample are also provided.

摘要翻译： 提供了一种用于诊断受试者HPV感染的方法。 可以通过产生来自受试者的生物样品的质量分布数据来确定受试者中的HPV感染，并将质量分布数据与参考质量分布相关联，以检测与HPV感染有关的至少一种生物标志物的存在或不存在和/或数量 。 还提供了用于检测生物样品中与HPV感染相关联的至少一种生物标志物的方法。

公开(公告)号：US5567591A

公开(公告)日：1996-10-22

申请号：US289833

申请日：1994-08-12

申请人： Stephen J. Lovell ,  Jeffrey H. Bruton

发明人： Stephen J. Lovell ,  Jeffrey H. Bruton

IPC分类号： G01N33/543 ,  G01N33/538

CPC分类号： G01N33/543 ,  G01N33/5432 ,  G01N2333/22 ,  Y10S435/81 ,  Y10S435/969 ,  Y10S436/829

摘要： An indirect assay for analyte employs a particle derivatized with a plurality of molecules of one or more compounds to increase assay sensitivity. In an indirect sandwich assay format, at least one of the compounds is a binder for the analyte, and the tracer is comprised of a binder for at least one of the compounds on the particle. In this manner, a plurality of tracer molecules may be indirectly bound to a single analyte molecule which is bound in a complex formed in the assay.

摘要翻译： 用于分析物的间接测定使用由多个分子的一种或多种化合物衍生的颗粒以提高测定灵敏度。 在间接夹心测定形式中，至少一种化合物是分析物的粘合剂，示踪剂由颗粒上至少一种化合物的粘合剂组成。 以这种方式，多个示踪剂分子可以间接地结合到在分析中形成的复合物中结合的单一分析物分子。

公开(公告)号：US20090269732A1

公开(公告)日：2009-10-29

申请号：US12108550

申请日：2008-04-24

申请人： Richard Ivey ,  Stephen J. Lovell ,  Robert Rosenstein ,  Thomas Gentle ,  Song Shi

发明人： Richard Ivey ,  Stephen J. Lovell ,  Robert Rosenstein ,  Thomas Gentle ,  Song Shi

IPC分类号： C12Q1/70

CPC分类号： G01N33/56983 ,  G01N2333/025

摘要： Methods for diagnosis of HPV infection in a subject are provided. HPV infection in a subject can be determined by generating mass profile data for a biological sample from the subject and correlating the mass profile data with reference mass profiles to detect the presence or absence, and/or quantity of at least one biomarker associated with HPV infection. Methods for detecting at least one biomarker associated with HPV infection in a biological sample are also provided.

摘要翻译： 提供了一种用于诊断受试者HPV感染的方法。 可以通过产生来自受试者的生物样品的质量分布数据来确定受试者中的HPV感染，并将质量分布数据与参考质量分布相关联，以检测与HPV感染有关的至少一种生物标志物的存在或不存在和/或数量 。 还提供了用于检测生物样品中与HPV感染相关联的至少一种生物标志物的方法。

公开(公告)号：US20080014657A1

公开(公告)日：2008-01-17

申请号：US11463903

申请日：2006-08-11

申请人： Stephen J. Lovell ,  Kenneth A. Kopher

发明人： Stephen J. Lovell ,  Kenneth A. Kopher

IPC分类号： G01N33/558

CPC分类号： G01N33/558 ,  C12Q1/06 ,  G01N33/54366 ,  G01N33/56944

摘要： Devices, kits, and methods are provided for analyzing a sample for the presence or amount of a bacterium in a sample, and for determining whether or not conditions present while practicing the invention allow for accurate test results. Specifically, the invention provides devices, kits, and methods for analyzing a sample for the presence or amount of a bacterial carbohydrate, while using an analog of the bacterial carbohydrate as a control reagent. In certain embodiments, the invention may provide for analyzing a sample for the presence or amount of a Group A Streptococcus (GAS) bacterium, by determining the presence or amount of β-N-Acetyl-D-Glucosamine attached to a poly-rhamnose backbone, a bacterial carbohydrate found in GAS, in the sample, and by using Albumin, Bovine, p-Aminophenyl N-Acetyl B-D Glucosaminide, an analog to β-N-Acetyl-D-Glucosamine as a control reagent.

摘要翻译： 提供了装置，试剂盒和方法，用于分析样品中样品中细菌的存在或数量，并确定在实施本发明时存在的条件是否允许准确的测试结果。 具体地，本发明提供了用于分析样品中细菌碳水化合物的存在或量的装置，试剂盒和方法，同时使用细菌碳水化合物的类似物作为对照试剂。 在某些实施方案中，本发明可以通过测定连接到聚鼠李糖骨架上的β-N-乙酰基-D-葡糖胺的存在或量来提供对A型链球菌（GAS）细菌的存在或量的样品的分析 ，在样品中的GAS中发现的细菌碳水化合物，以及通过使用白蛋白，牛，对氨基苯基N-乙酰基BD葡糖胺，类似于β-N-乙酰基-D-葡糖胺作为对照试剂。

公开(公告)号：US5989924A

公开(公告)日：1999-11-23

申请号：US941184

申请日：1997-09-30

申请人： Richard T. Root ,  Stephen J. Lovell

发明人： Richard T. Root ,  Stephen J. Lovell

IPC分类号： G01N33/543 ,  G01N33/5588

CPC分类号： G01N33/54366 ,  Y10S435/81 ,  Y10S436/805 ,  Y10S436/809 ,  Y10S436/81

摘要： The present invention relates to a device for determining an analyte in a fluid sample in an assay. The device comprises a lid having a plurality of elements and a membrane. The elements have openings which allow passage of fluid to the membrane. The membrane is supported by the lid and covers the openings of the elements. The undersurface of the membrane is in contact with a porous wick which contains a first zone which includes binder attached to a particulate label and a second zone containing salt. The wick extends downward from the membrane and is in contact with the fluid sample.

摘要翻译： 本发明涉及用于在测定中测定流体样品中分析物的装置。 该装置包括具有多个元件和膜的盖。 这些元件具有允许流体通过膜的开口。 膜由盖支撑并覆盖元件的开口。 膜的下表面与多孔芯接触，多孔芯包括第一区，其包括附着于颗粒标签的粘合剂和含有盐的第二区。 芯从膜向下延伸并与流体样品接触。

公开(公告)号：US5834217A

公开(公告)日：1998-11-10

申请号：US763858

申请日：1996-12-11

申请人： Robert A. Levine ,  Stephen C. Wardlaw ,  Leon W. M. M. Terstappen ,  Kristen L. Manion ,  Rodolfo R. Rodriguez ,  Adrien P. Malick ,  Subhash Dhanesar ,  Stephen J. Lovell ,  Alvydas J. Ozinskas

发明人： Robert A. Levine ,  Stephen C. Wardlaw ,  Leon W. M. M. Terstappen ,  Kristen L. Manion ,  Rodolfo R. Rodriguez ,  Adrien P. Malick ,  Subhash Dhanesar ,  Stephen J. Lovell ,  Alvydas J. Ozinskas

IPC分类号： G01N33/543 ,  B01L3/14 ,  G01N33/49 ,  G01N33/537 ,  G01N33/569 ,  G01N33/58 ,  G01N33/546

CPC分类号： B01L3/5021 ,  G01N33/491 ,  G01N33/5375 ,  G01N33/56972 ,  G01N33/585 ,  Y10S435/81 ,  Y10S435/967 ,  Y10S435/971 ,  Y10S435/973 ,  Y10S436/805 ,  Y10S436/81 ,  Y10S436/824 ,  Y10S436/829 ,  Y10T436/111666

摘要： A patient's health may be diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more bodies or groups of bodies such as floats, inserts, liposomes, or plastic beads of different densities. Each density-defined body carries analyte-capture binding materials such as antigens or antibodies, which are specific to an epitope, or other specific high affinity binding site on a target analyte which target analyte may be in the blood or other sample being tested; and the level of which analyte is indicative of the patient's health. At least one labeled binding material which is also specific to an epitope, or other specific high affinity binding site on the target analyte is added to the sample so as to form labeled binding material/analyte/body complexes in the sample. Upon centrifugation, the complexes will settle out in different areas in the tube according to the respective density of the body or bodies; and the degree of label emission of the complex layers can enable qualitative and/or quantitative analyses of the sample to be made. Unbound labeled binding materials will be separated from the complexed layers by the washing action of ascending or descending components of the sample during the centrifugation step. Unbound labeled binding material will thus not interfere with the analysis.