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    Method and apparatus for aligning microbeads in order to interrogate the same
    3.
    发明授权
    Method and apparatus for aligning microbeads in order to interrogate the same 有权
    用于对准微珠以便询问它的方法和装置

    公开(公告)号：US07399643B2

    公开(公告)日：2008-07-15

    申请号：US10661836

    申请日：2003-09-12

    申请人： John Moon ,  Martin A. Putnam ,  Tuo Li ,  Joseph Traynor

    发明人： John Moon ,  Martin A. Putnam ,  Tuo Li ,  Joseph Traynor

    IPC分类号： G01N33/552

    CPC分类号： G03H1/0272 ,  B01J2219/00459 ,  B01J2219/00463 ,  B01J2219/0047 ,  B01J2219/005 ,  B01J2219/00547 ,  B01J2219/00576 ,  B01J2219/00702 ,  B01L3/502761 ,  B01L3/5085 ,  B01L2200/0636 ,  B01L2200/0668 ,  B01L2300/0654 ,  B01L2400/0409 ,  B01L2400/0457 ,  B01L2400/0487 ,  G03H2210/53 ,  G03H2230/10 ,  G03H2270/20 ,  G03H2270/24 ,  Y10T436/25

    摘要： A method and apparatus are provided for aligning optical elements or microbeads, wherein each microbead has an elongated body with a code embedded therein along a longitudinal axis thereof to be read by a code reading device. The microbeads are aligned with a positioning device so the longitudinal axis of the microbeads is positioned in a fixed orientation relative to the code reading device. The microbeads are typically cylindrically shaped glass beads between 25 and 250 microns (μm) in diameter and between 100 and 500 μm long, and have a holographic code embedded in the central region of the bead, which is used to identify it from the rest of the beads in a batch of beads with many different chemical probes. A cross reference is used to determine which probe is attached to which bead, thus allowing the researcher to correlate the chemical content on each bead with the measured fluorescence signal. Because the code consists of a diffraction grating typically disposed along an axis, there is a particular alignment required between the incident readout laser beam and the readout detector in two of the three rotational axes. The third axis, rotation about the center axis of the cylinder, is azimuthally symmetric and therefore does not require alignment.

    摘要翻译： 提供了一种用于对准光学元件或微珠的方法和装置，其中每个微珠具有细长体，其沿着其纵向轴线嵌入其中的代码，以被代码读取装置读取。 微珠与定位装置对准，使得微珠的纵向轴线相对于代码读取装置定位成固定的取向。 微珠通常为直径为25至250微米（母体）和长度为100至500毫米的圆柱形玻璃珠，并且具有嵌入珠的中心区域中的全息代码，其用于从其余部分识别它 珠子中有一批具有许多不同化学探针的珠粒。 交叉参考用于确定哪个探针连接到哪个珠，从而允许研究者将每个珠上的化学成分与测量的荧光信号相关联。 由于代码由通常沿轴线设置的衍射光栅组成，因此在三个旋转轴中的两个中存在入射读出激光束和读出检测器之间所需的特定对准。 第三轴，围绕气缸的中心轴的旋转是方位对称的，因此不需要对准。

    Hybrid random bead/chip based microarray
    7.
    发明授权
    Hybrid random bead/chip based microarray 有权
    混合随机珠/芯片基微阵列

    公开(公告)号：US07164533B2

    公开(公告)日：2007-01-16

    申请号：US10763995

    申请日：2004-01-22

    申请人： John Moon ,  Alan D. Kersey ,  Martin A. Putnam ,  Tuo Li

    发明人： John Moon ,  Alan D. Kersey ,  Martin A. Putnam ,  Tuo Li

    IPC分类号： G02B5/18

    CPC分类号： G01N33/552 ,  G01N21/4788 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/7743 ,  G01N33/54306 ,  G01N33/54313 ,  G01N33/54366 ,  G01N33/54386 ,  G03H1/0005 ,  G03H1/02 ,  G03H1/0236 ,  G03H1/26 ,  G03H2001/0033 ,  G03H2001/0044 ,  G03H2210/53 ,  G03H2210/63 ,  G03H2230/10 ,  G03H2250/12 ,  G06K19/04 ,  G06K19/06009

    摘要： A method and apparatus f or performing an assay process, featuring providing microbeads in a solution; placing the microbeads on an alignment substrate; reading codes of the microbeads and the position thereof on the alignment substrate; reading the fluorescence on each microbead and the position order thereof on the alignment substrate; and determining an assay result based on bead position order and bead code of the earlier reading steps, where the microbead is an encoded particle having a particle substrate; a portion of the substrate being made of a substantially single material and having at least one diffraction grating embedded therein, the grating having a resultant refractive index variation within the single material at a grating location; and the grating providing an optical output signal indicative of a code when illuminated by an incident light signal propagating from outside said substrate, the optical output signal being a result of passive, non-resonant scattering from said grating when illuminated by said incident light signal.

    摘要翻译： f或执行测定过程的方法和装置，其特征在于在溶液中提供微珠; 将微珠放置在取向基板上; 读取微珠的代码及其位置; 读取每个微珠上的荧光及其在取向基板上的位置顺序; 并且基于早期阅读步骤的珠粒位置顺序和珠粒码确定测定结果，其中微珠是具有颗粒底物的编码颗粒; 衬底的一部分由基本上单一的材料制成并且具有嵌入其中的至少一个衍射光栅，所述光栅在光栅位置处具有单个材料内的折射率变化; 并且所述光栅提供指示当通过从所述衬底外部传播的入射光信号照射时的代码的光学输出信号，所述光学输出信号是当被所述入射光信号照亮时来自所述光栅的被动的非共振散射的结果。

    Multi-well plate with alignment grooves for encoded microparticles
    9.
    发明申请
    Multi-well plate with alignment grooves for encoded microparticles 审中-公开
    具有用于编码微粒的对准槽的多孔板

    公开(公告)号：US20060160208A1

    公开(公告)日：2006-07-20

    申请号：US11063665

    申请日：2005-02-22

    申请人： Martin Putnam ,  James Sirkis ,  John Moon ,  Tuo Li ,  Joseph Traynor ,  Robert Brucato

    发明人： Martin Putnam ,  James Sirkis ,  John Moon ,  Tuo Li ,  Joseph Traynor ,  Robert Brucato

    IPC分类号： C12M1/34 ,  G06K7/00 ,  G01N33/551 ,  C12M3/00

    CPC分类号： G01N33/54313 ,  G01N33/54393

    摘要： A method and apparatus are provided for aligning optical elements or microbeads, wherein each microbead has an elongated body with a code embedded therein along a longitudinal axis thereof to be read by a code reading device. The microbeads are aligned with a positioning device so the longitudinal axis of the microbeads is positioned in a fixed orientation relative to the code reading device. The microbeads are typically cylindrically shaped glass beads between 25 and 250 microns (μm) in diameter and between 100 and 500 μm long, and have a holographic code embedded in the central region of the bead, which is used to identify it from the rest of the beads in a batch of beads with many different chemical probes. A cross reference is used to determine which probe is attached to which bead, thus allowing the researcher to correlate the chemical content on each bead with the measured fluorescence signal. Because the code consists of a diffraction grating typically disposed along an axis, there is a particular alignment required between the incident readout laser beam and the readout detector in two of the three rotational axes. The third axis, rotation about the center axis of the cylinder, is azimuthally symmetric and therefore does not require alignment.

    摘要翻译： 提供了一种用于对准光学元件或微珠的方法和装置，其中每个微珠具有细长体，其沿着其纵向轴线嵌入其中的代码，以被代码读取装置读取。 微珠与定位装置对准，使得微珠的纵向轴线相对于代码读取装置定位成固定的取向。 微珠通常为直径为25至250微米（母体）和长度为100至500毫米的圆柱形玻璃珠，并且具有嵌入珠的中心区域中的全息代码，其用于从其余部分识别它 珠子中有一批具有许多不同化学探针的珠粒。 交叉参考用于确定哪个探针连接到哪个珠，从而允许研究者将每个珠上的化学成分与测量的荧光信号相关联。 由于代码由通常沿轴线设置的衍射光栅组成，所以在三个旋转轴中的两个中存在入射读出激光束和读出检测器之间所需的特定对准。 第三轴，围绕气缸的中心轴的旋转是方位对称的，因此不需要对准。

    Diffraction grating-based encoded microparticle assay stick
    10.
    发明授权
    Diffraction grating-based encoded microparticle assay stick 有权
    基于衍射光栅的编码微粒测定棒

    公开(公告)号：US07901630B2

    公开(公告)日：2011-03-08

    申请号：US11226914

    申请日：2005-09-13

    申请人： Martin A. Putnam ,  John A. Moon ,  Tuo Li

    发明人： Martin A. Putnam ,  John A. Moon ,  Tuo Li

    IPC分类号： G01N33/00 ,  G01N33/556 ,  G03H1/00 ,  C12M1/34 ,  C12M3/00

    CPC分类号： G01N21/4788 ,  G01N21/6428 ,  G03H1/26 ,  G03H2001/0033

    摘要： An assay stick 7 includes a transparent reaction vessel or tube 14 having one or more microbeads 8 disposed therein. The microbeads 8 have a plurality of unique identification digital codes based on a diffraction grating 12 disposed therein that are detected when illuminated by incident light 24. The incident light 24 may be directed transversely onto the side or onto an end of the tube 14 with a narrow band (single wavelength) or multiple wavelength source, in which case the code is represented by a spatial distribution of light or a wavelength spectrum, respectively. The assay stick 7 may be reused or disposed upon completion of the assay. Alternatively, the beads may be attached to a strip or planar surface. The encoded beads can also provide traceability, quality-control, and authenticity of each bead 8 to its source and/or to the chemistry on each bead 8. Also, the low sample volume of the assay stick allows for faster reactions and better sensitivity.

    摘要翻译： 测定棒7包括其中设置有一个或多个微珠8的透明反应容器或管14。 微珠8具有基于设置在其中的衍射光栅12的多个独特的识别数字代码，其被入射光24照射时检测。入射光24可以横向地被引导到管14的一侧或一端上，其中 窄带（单波长）或多波长源，在这种情况下，码分别由光或波长光谱的空间分布表示。 测定棒7可以在测定完成后重复使用或放置。 或者，珠可以附接到条或平面表面。 编码的珠粒还可以提供每个珠粒8到其来源和/或每个珠粒8上的化学物质的可追溯性，质量控制和真实性。而且，测定棒的低样本体积允许更快的反应和更好的灵敏度。