公开(公告)号：US06333007B1

公开(公告)日：2001-12-25

申请号：US09612484

申请日：2000-07-07

申请人： Johnny Svensson ,  Bertil Nilsson ,  Per Olsson ,  Lars Jansson ,  Bo Jönsson

发明人： Johnny Svensson ,  Bertil Nilsson ,  Per Olsson ,  Lars Jansson ,  Bo Jönsson

IPC分类号： G01N2103

CPC分类号： B01F11/0266 ,  B01F13/0059 ,  G01N21/03 ,  G01N2021/0346

摘要： A photometer for determining the transmission of a liquid sample in a microcuvette (7, 20, 22) has a cavity for a capillary layer of the liquid sample with a free liquid surface extended transversely of the principal plane of the cavity. The cavity is initially prepared with a reagent to achieve a reaction, which affects the transmission of the liquid sample for indicating its contents of a predetermined substance. The photometer has a holder (6) for the microcuvette and measuring means (9, 10) for measuring the transmission of a bundle of rays directed towards the microcuvette. The cuvette holder (6) is mounted on bearings for vibration in a direction having a component, which extends in the plane of the free liquid surface and is parallel with the principal plane of the cavity, thereby achieving a mixing to accelerate the dissolution and the reaction.

摘要翻译： 用于确定液体样品在微型小瓶（7,20,22）中的透射的光度计具有用于液体样品的毛细管层的空腔，其具有横向于腔的主平面延伸的自由液体表面。 最初用试剂制备空腔以实现反应，其影响用于指示其预定物质的内容物的液体样品的透射。 光度计具有用于微型检测器的保持器（6）和测量装置（9,10），用于测量朝向微型检查器的射线束的透射。 将试管支架（6）安装在轴承上，以便在具有部分的方向上振动，该部件在自由液体表面的平面中延伸并与空腔的主平面平行，从而实现混合以加速溶解和 反应。

公开(公告)号：US06468807B1

公开(公告)日：2002-10-22

申请号：US09612273

申请日：2000-07-07

申请人： Johnny Svensson ,  Bertil Nilsson ,  Per Olsson ,  Lars Jansson

发明人： Johnny Svensson ,  Bertil Nilsson ,  Per Olsson ,  Lars Jansson

IPC分类号： G01N33543

CPC分类号： G01N21/03 ,  B01F13/0059 ,  B01L3/50273 ,  B01L2200/026 ,  B01L2300/0825 ,  B01L2400/0406 ,  B01L2400/0457 ,  G01N21/11 ,  G01N2021/0325 ,  G01N2021/0328 ,  G01N2021/0346 ,  Y10S436/827

摘要： The invention relates to a method for carrying out mixing in a thin liquid layer, which is arranged between essentially parallel walls at a capillary distance from each other. The mixing is carried out by subjecting the walls to a motion essentially in the plane of the liquid layer, balancing the motion against the capillary force exerted by the walls on the liquid, and selecting the interface between the liquid layer and the surrounding medium so that it functions as an elastic membrane. The invention also concerns a cuvette which is designed for mixing according to the method.

摘要翻译： 本发明涉及一种在薄液层中进行混合的方法，该薄液层布置在彼此毛细距离的基本上平行的壁之间。 混合是通过使壁基本上在液体层的平面中进行运动来实现的，该运动平衡了由液体上的壁施加的毛细管力的运动，并且选择液体层和周围介质之间的界面，使得 它用作弹性膜。 本发明还涉及一种根据该方法设计用于混合的试管。

公开(公告)号：US20060210428A1

公开(公告)日：2006-09-21

申请号：US11102837

申请日：2005-04-11

申请人： Stellan Lindberg ,  Johnny Svensson

发明人： Stellan Lindberg ,  Johnny Svensson

IPC分类号： G01N31/22

CPC分类号： G01N21/03 ,  A61B5/150022 ,  A61B5/15003 ,  A61B5/150099 ,  A61B5/150229 ,  A61B5/150343 ,  A61B5/150755 ,  A61B5/157 ,  G01N15/1475 ,  G01N15/1484 ,  G01N21/11 ,  G01N2015/1486 ,  G01N2021/0321 ,  G01N2021/0325 ,  G01N2021/0346 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/13

摘要： A sample acquiring device for volumetric enumeration of white blood cells in a blood sample comprises a measurement cavity for receiving a blood sample. The measurement cavity has a predetermined fixed thickness. The sample acquiring device further comprises a reagent, which is arranged in a dried form on a surface defining the measurement cavity. The reagent comprises a hemolysing agent for lysing red blood cells in the blood sample, and a staining agent for selectively staining white blood cells in the blood sample. A system comprises the sample acquiring device and a measurement apparatus. The measurement apparatus comprises a sample acquiring device holder, a light source, and an imaging system for acquiring a digital image of a magnification of the sample. The measurement apparatus further comprises an image analyser arranged to analyse the acquired digital image for determining the number of white blood cells in the blood sample.

摘要翻译： 用于在血液样本中体积计数白细胞的样本获取装置包括用于接收血液样本的测量腔。 测量空腔具有预定的固定厚度。 样品获取装置还包括在限定测量空腔的表面上以干燥形式布置的试剂。 该试剂包含用于裂解血液样品中的红细胞的溶血剂，以及用于选择性地染色血液样品中的白细胞的染色剂。 系统包括样本获取装置和测量装置。 测量装置包括样本获取装置保持器，光源和用于获取样本的放大倍数的数字图像的成像系统。 测量装置还包括一个图像分析器，被配置为分析所获取的数字图像以确定血液样本中的白细胞数。

公开(公告)号：US06831733B2

公开(公告)日：2004-12-14

申请号：US10323755

申请日：2002-12-20

申请人： Joakim Pettersson ,  Johnny Svensson

发明人： Joakim Pettersson ,  Johnny Svensson

IPC分类号： G01N3348

CPC分类号： G01N21/31

摘要： The invention concerns a method for quantitative hemoglobin determination in undiluted, unhemolyzed whole blood comprising the steps of: providing a disposable, capillary, which has an optical path length of less than 1 mm; filling said cuvette with a sample of unaltered whole blood; performing a first absorption measurement at a wavelength in the range 490-520 nm directly on the sample in the cuvette, and further conducting a second absorption measurement, and processing results of the first and second absorption measurements to determine the concentration of hemoglobin in the sample, wherein the step of processing comprises compensating for scattering in the sample, said compensating being dependent on the result of the second absorption measurement. A system for implementing the method is also disclosed.

摘要翻译： 本发明涉及一种在未稀释的未溶血全血中进行定量血红蛋白测定的方法，包括以下步骤：提供具有小于1mm光程长度的一次性毛细管;用未改变的全血样品填充所述比色皿;执行 在比色皿中的样品上直接在490-520nm范围内的波长进行第一次吸收测量，并进一步进行第二吸收测量，以及处理第一和第二吸收测量结果以确定样品中血红蛋白的浓度，其中 处理步骤包括补偿样品中的散射，所述补偿取决于第二吸收测量的结果。还公开了用于实现该方法的系统。

公开(公告)号：US08092758B2

公开(公告)日：2012-01-10

申请号：US11885637

申请日：2006-03-10

申请人： Stellan Lindberg ,  Johnny Svensson

发明人： Stellan Lindberg ,  Johnny Svensson

IPC分类号： G01N33/52 ,  G01N33/00

CPC分类号： G01N21/03 ,  A61B5/150022 ,  A61B5/15003 ,  A61B5/150099 ,  A61B5/150229 ,  A61B5/150343 ,  A61B5/150755 ,  A61B5/157 ,  G01N15/1475 ,  G01N15/1484 ,  G01N21/11 ,  G01N2015/1486 ,  G01N2021/0321 ,  G01N2021/0325 ,  G01N2021/0346 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/13

摘要： A sample acquiring device for volumetric enumeration of white blood cells in a blood sample comprises a measurement cavity for receiving a blood sample. The measurement cavity has a predetermined fixed thickness. The sample acquiring device further comprises a reagent, which is arranged in a dried form on a surface defining the measurement cavity. The reagent comprises a hemolysing agent for lysing red blood cells in the blood sample, and a staining agent for selectively staining white blood cells in the blood sample. A system comprises the sample acquiring device and a measurement apparatus. The measurement apparatus comprises a sample acquiring device holder, a light source, and an imaging system for acquiring a digital image of a magnification of the sample. The measurement apparatus further comprises an image analyser arranged to analyse the acquired digital image for determining the number of white blood cells in the blood sample.

摘要翻译： 用于在血液样本中体积计数白细胞的样本获取装置包括用于接收血液样本的测量腔。 测量空腔具有预定的固定厚度。 样品获取装置还包括在限定测量空腔的表面上以干燥形式布置的试剂。 该试剂包含用于裂解血液样品中的红细胞的溶血剂，以及用于选择性地染色血液样品中的白细胞的染色剂。 系统包括样本获取装置和测量装置。 测量装置包括样本获取装置保持器，光源和用于获取样本的放大倍数的数字图像的成像系统。 测量装置还包括一个图像分析器，被配置为分析所获取的数字图像以确定血液样本中的白细胞数。

公开(公告)号：US07521243B2

公开(公告)日：2009-04-21

申请号：US11102837

申请日：2005-04-11

申请人： Stellan Lindberg ,  Johnny Svensson

发明人： Stellan Lindberg ,  Johnny Svensson

IPC分类号： G01N31/00

CPC分类号： G01N21/03 ,  A61B5/150022 ,  A61B5/15003 ,  A61B5/150099 ,  A61B5/150229 ,  A61B5/150343 ,  A61B5/150755 ,  A61B5/157 ,  G01N15/1475 ,  G01N15/1484 ,  G01N21/11 ,  G01N2015/1486 ,  G01N2021/0321 ,  G01N2021/0325 ,  G01N2021/0346 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/13

摘要： A sample acquiring device for volumetric enumeration of white blood cells in a blood sample that includes a measurement cavity for receiving a blood sample. The measurement cavity has a predetermined fixed thickness. The sample acquiring device also has a reagent, which is arranged in a dried form on a surface defining the measurement cavity. The reagent has a hemolysing agent for lysing red blood cells in the sample and a staining agent for selectively staining white blood cells in the sample. Also, a system with the sample acquiring device and a measurement apparatus. The measurement apparatus has a sample acquiring device holder, a light source, and an imaging system for acquiring a digital image of a magnification of the sample The measurement apparatus also has an image analyzer arranged to analyze the acquired digital image for determining the number of white blood cells in the blood sample.

摘要翻译： 一种用于在血液样本中体积计数白细胞的样本获取装置，其包括用于接收血液样本的测量腔。 测量空腔具有预定的固定厚度。 样品获取装置还具有在限定测量空腔的表面上以干燥形式布置的试剂。 该试剂具有用于裂解样品中红细胞的溶血剂和用于选择性染色样品中白细胞的染色剂。 而且，具有采样装置和测量装置的系统。 测量装置具有取样装置保持器，光源以及用于获取样本的倍数的数字图像的摄像系统。测量装置还具有图像分析装置，被配置为分析所获取的数字图像以确定白色的数量 血液样本中的血细胞。

公开(公告)号：US20080160566A1

公开(公告)日：2008-07-03

申请号：US11885637

申请日：2006-03-10

申请人： Stellan Lindberg ,  Johnny Svensson

发明人： Stellan Lindberg ,  Johnny Svensson

IPC分类号： C12M1/34 ,  C12Q1/20 ,  G06K9/00

CPC分类号： G01N21/03 ,  A61B5/150022 ,  A61B5/15003 ,  A61B5/150099 ,  A61B5/150229 ,  A61B5/150343 ,  A61B5/150755 ,  A61B5/157 ,  G01N15/1475 ,  G01N15/1484 ,  G01N21/11 ,  G01N2015/1486 ,  G01N2021/0321 ,  G01N2021/0325 ,  G01N2021/0346 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/13

摘要： A sample acquiring device for volumetric enumeration of white blood cells in a blood sample comprises a measurement cavity for receiving a blood sample. The measurement cavity has a predetermined fixed thickness. The sample acquiring device further comprises a reagent, which is arranged in a dried form on a surface defining the measurement cavity. The reagent comprises a hemolysing agent for lysing red blood cells in the blood sample, and a staining agent for selectively staining white blood cells in the blood sample. A system comprises the sample acquiring device and a measurement apparatus. The measurement apparatus comprises a sample acquiring device holder, a light source, and an imaging system for acquiring a digital image of a magnification of the sample. The measurement apparatus further comprises an image analyser arranged to analyse the acquired digital image for determining the number of white blood cells in the blood sample.

摘要翻译： 用于在血液样本中体积计数白细胞的样本获取装置包括用于接收血液样本的测量腔。 测量空腔具有预定的固定厚度。 样品获取装置还包括在限定测量空腔的表面上以干燥形式布置的试剂。 该试剂包含用于裂解血液样品中的红细胞的溶血剂，以及用于选择性地染色血液样品中的白细胞的染色剂。 系统包括样本获取装置和测量装置。 测量装置包括样本获取装置保持器，光源和用于获取样本的放大倍数的数字图像的成像系统。 测量装置还包括一个图像分析器，被配置为分析所获取的数字图像以确定血液样本中的白细胞数。

公开(公告)号：US06638769B2

公开(公告)日：2003-10-28

申请号：US09972918

申请日：2001-10-10

申请人： Jan Lilja ,  Sven-Erik Nilsson ,  Johnny Svensson ,  Annika Eriksson

发明人： Jan Lilja ,  Sven-Erik Nilsson ,  Johnny Svensson ,  Annika Eriksson

IPC分类号： G01N3372

CPC分类号： G01N21/272 ,  G01N21/03 ,  G01N21/0303 ,  G01N21/31 ,  G01N33/5094 ,  G01N33/72 ,  G01N2021/0321 ,  G01N2021/0325 ,  G01N2021/0346 ,  G01N2021/0389

摘要： A method for hemoglobin determination is described which includes the steps of introducing a sample of undiluted whole blood by capillary action into a disposable microcuvette having at least one cavity for receiving the sample. The cavity includes a dry essentially non-hygroscopic hemolysing agent, which is dissolved by the blood, hemolyses the red blood cells and releases the haemoglobin contained in the blood cells. A first absorption measurement at a wavelength range 490-520 nm is then performed directly on the sample in the microcuvette, and a second absorption measurement is performed to compensate for background interference.

摘要翻译： 描述了一种用于血红蛋白测定的方法，其包括以下步骤：通过毛细管作用将未稀释的全血样品引入到具有用于接收样品的至少一个空腔的一次性微型小瓶中。 空腔包括干燥的基本上不吸湿的溶血剂，其被血液溶解，溶血红细胞并释放血细胞中所含的血红蛋白。 然后直接在微量检测器中的样品上进行490-520nm波长范围的第一次吸收测量，并进行第二次吸收测量以补偿背景干扰。