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公开(公告)号：US06265387B1
公开(公告)日：2001-07-24
申请号：US08975573
申请日：1997-11-21
IPC分类号： A61K3500
CPC分类号： A61K48/00 , A61K48/0008
摘要： A process for delivering a polynucleotide to a parenchymal cell in a mammal by injecting the polynucleotide into a blood vessel connected to the parenchymal cell in tissue or organ of the mammal such that the polynucleotide is transfected into the parenchymal cell and functionally expressed to therapeutic levels.
摘要翻译： 将多核苷酸通过将多核苷酸注射到与哺乳动物的组织或器官中的实质细胞连接的血管中将多核苷酸递送至实质细胞的方法，使得多核苷酸被转染到实质细胞中并在功能上表达为治疗水平。
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公开(公告)号：US6093701A
公开(公告)日：2000-07-25
申请号：US990015
申请日：1997-12-12
CPC分类号： A61K47/48238 , A61K48/00
摘要： The described invention relates to methods for covalently attaching a compound to a gene. The method provides for covalently attaching compounds to genes for enhancing the cellular transport of the genes to predetermined targets, while maintaining the gene's functionality.
摘要翻译： 所述发明涉及将化合物共价连接至基因的方法。 该方法提供了将基因共价连接到基因上，以增强基因到预定靶标的细胞转运，同时保持基因的功能。
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公开(公告)号：US06593465B1
公开(公告)日：2003-07-15
申请号：US09767794
申请日：2001-01-23
IPC分类号： C07H2100
CPC分类号： C07H21/00
摘要： Compounds and methods are provided for a single-step covalent attachment of a label to a molecule comprising forming a covalently attachable labeling reagent for alkylating the molecule. Then, combining the covalently attachable labeling reagent with a mixture containing the molecule, under conditions wherein the labeling reagent has reactivity with the molecule thereby forming a covalent bond.
摘要翻译： 提供了化合物和方法用于将标记物与分子的单步共价连接，包括形成用于烷基化分子的共价连接的标记试剂。 然后，在标记试剂与分子具有反应性，从而形成共价键的条件下，将可共价连接的标记试剂与含有该分子的混合物结合。
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4.发明授权Single-step method for labeling nucleic acids with mustard or aziridine labeling reagents 失效用芥末或氮丙啶标记试剂标记核酸的单步方法公开(公告)号：US06262252B1
公开(公告)日：2001-07-17
申请号：US08982485
申请日：1997-12-02
IPC分类号： C07H2100
CPC分类号： C07H21/00
摘要： Compounds and methods are provided for a single-step covalent attachment of a label to a molecule comprising forming a covalently attachable labeling reagent for alkylating the molecule. Then, combining the covalently attachable labeling reagent with a mixture containing the molecule, under conditions wherein the labeling reagent has reactivity with the molecule thereby forming a covalent bond.
摘要翻译： 提供了化合物和方法用于将标记物与分子的单步共价连接，包括形成用于烷基化分子的共价连接的标记试剂。 然后，在标记试剂与分子具有反应性，从而形成共价键的条件下，将可共价连接的标记试剂与含有该分子的混合物结合。
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5.发明授权Process of transfecting a cell with a polynucleotide mixed with an amphipathic compound and a DNA-binding protein 失效用与两亲化合物和DNA结合蛋白质混合的多核苷酸转染细胞的方法
公开(公告)号：US5744335A
公开(公告)日：1998-04-28
申请号：US530598
申请日：1995-09-19
申请人： Jon A. Wolff , Jeffery Fritz , Vladimir Budker , James Hagstrom
发明人： Jon A. Wolff , Jeffery Fritz , Vladimir Budker , James Hagstrom
CPC分类号： C12N15/87
摘要： The present invention provides a process of transfecting a cell with a polynucleotide mixed with one or more amphipathic compounds and an effective amount of a DNA-binding protein. Exemplary and preferred DNA-binding proteins are H1, H2A, and H2B. Exemplary and preferred amphipathic compounds are cationic amphipathic compounds.
摘要翻译： 本发明提供了用与一种或多种两亲化合物和有效量的DNA结合蛋白混合的多核苷酸转染细胞的方法。 示例性和优选的DNA结合蛋白是H1，H2A和H2B。 示例性和优选的两亲性化合物是阳离子两亲性化合物。
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6.发明授权Compositions and methods for drug delivery using amphiphile binding molecules 失效使用两亲性结合分子的药物递送的组合物和方法公开(公告)号：US06740643B2
公开(公告)日：2004-05-25
申请号：US09726792
申请日：2000-11-29
申请人： Jon A. Wolff , James E. Hagstrom , Sean D. Monahan , Vladimir Budker , David B. Rozema , Paul M. Slattum
发明人： Jon A. Wolff , James E. Hagstrom , Sean D. Monahan , Vladimir Budker , David B. Rozema , Paul M. Slattum
IPC分类号： A61K4800
CPC分类号： C12N15/87 , A61K47/6951 , A61K48/00 , A61K48/0025 , B82Y5/00 , C07H21/00
摘要： The present invention relates to the delivery of desired compounds (e.g., nucleic acids) into cells using noncovalent delivery systems which include complexing nucleic acids, amphipathic binding agents, and amphiphiles.
摘要翻译： 本发明涉及使用非共价递送系统将期望的化合物（例如核酸）递送到细胞中，其包括络合的核酸，两亲性结合剂和两亲物。
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7.发明授权Amphipathic PH sensitive compounds and delivery systems for delivering biologically active compounds 失效两亲PH敏感化合物和用于递送生物活性化合物的递送系统
公开(公告)号：US5965434A
公开(公告)日：1999-10-12
申请号：US365841
申请日：1994-12-29
申请人： Jon A. Wolff , Vladimir Budker , Vladimir Gurevich
发明人： Jon A. Wolff , Vladimir Budker , Vladimir Gurevich
IPC分类号： A61K9/127 , C07C219/06 , C07C239/12 , C07C279/12 , C07D295/092 , C07D295/13 , C12N15/88 , C12N15/63
CPC分类号： C07D295/088 , A61K9/1272 , C07C219/06 , C07C239/12 , C07C279/12 , C07D295/13 , C12N15/88 , Y10T428/2984
摘要： The present invention provides amphipathic lipid compounds comprising a hydrophilic, catonic, pH-sensitive moiety, the positive charge of which moiety increases as pH decreases over the pH range of 8.0 to 4.5. Vesicular delivery systems comprising such amphipathic compounds and the use of those systems for delivering biologically active substances to cells are also provided.
摘要翻译： 本发明提供包含亲水性，结合性，pH敏感性部分的两亲性脂质化合物，当pH在8.0至4.5的pH范围内pH降低时，其部分的正电荷增加。 还提供了包含这种两亲化合物的囊泡递送系统和用于将生物活性物质递送到细胞的那些系统的用途。
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8.发明授权
公开(公告)号：US5635487A
公开(公告)日：1997-06-03
申请号：US368150
申请日：1994-12-29
申请人： Jon A. Wolff , Vladimir Budker , Vladimir Gurevich
发明人： Jon A. Wolff , Vladimir Budker , Vladimir Gurevich
IPC分类号： A61K9/107 , A61K9/127 , A61K48/00 , C07J31/00 , C07J41/00 , C07J43/00 , A61K31/415 , A61K31/66
CPC分类号： C07J41/0055 , A61K48/00 , A61K9/1075 , A61K9/1272 , C07J31/003 , C07J41/0066 , C07J43/003
摘要： The present invention provides a composition comprising a population of micelles wherein each micelle comprises at least one amphipathic compound layer that surrounds a non-aqueous core that contains a polyion. Also provided are a method of preparing such a composition and the uses of such compositions for delivering biologically active polyions to cells.
摘要翻译： 本发明提供一种包含一组胶束的组合物，其中每个胶束包含至少一个两性化合物层，其包围含有聚阴离子的非水性核心。 还提供了制备这种组合物的方法以及这种组合物用于将生物活性聚阴离子递送至细胞的用途。
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公开(公告)号：US06818626B1
公开(公告)日：2004-11-16
申请号：US09234606
申请日：1999-01-21
申请人： Jon A. Wolff , James E. Hagstrom , Sean D. Monahan , Vladimir Budker , Vladimir Trubetskoy , Paul M. Slattum
发明人： Jon A. Wolff , James E. Hagstrom , Sean D. Monahan , Vladimir Budker , Vladimir Trubetskoy , Paul M. Slattum
IPC分类号： A61K4800
CPC分类号： A61K48/0025 , A61K48/00 , C12N15/87
摘要： Chelator containing compounds are utilized in the delivery of molecules, polymers, nucleic acids and genes to animal cells. At least one chelator such as crown ether is attached to a polymer and then associated with another polymer such as DNA. An ion is then added to the mixture thereby forming condensed DNA. In condensed form and in complex with the chelator, DNA can be delivered to a cell.
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公开(公告)号：US20070071823A1
公开(公告)日：2007-03-29
申请号：US10095681
申请日：2002-03-11
CPC分类号： C12N15/88 , A61K47/58 , A61K47/59 , A61K47/60 , A61K47/645 , A61K48/00 , A61K48/0008 , A61K48/0025 , A61K48/0041 , C12N15/87
摘要： An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
摘要翻译： 为了向细胞递送核酸，将聚二聚物用于缩合的多核苷酸复合物中。 络合物可以用适量的正电荷和/或负电荷形成，使得所得复合物可以递送到血管外空间并且可以进一步递送至细胞。
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