公开(公告)号：US20100151057A1

公开(公告)日：2010-06-17

申请号：US12095007

申请日：2008-05-08

申请人： Jong-Bae Seo ,  Sun-Mi Choi ,  Eun-Jung Choi ,  Sang-Wook Park ,  Eun-Wook Choi ,  Dong-Seung Seen ,  Tae-Gyu Lee

发明人： Jong-Bae Seo ,  Sun-Mi Choi ,  Eun-Jung Choi ,  Sang-Wook Park ,  Eun-Wook Choi ,  Dong-Seung Seen ,  Tae-Gyu Lee

IPC分类号： A61K36/00 ,  A61P3/04 ,  A61K8/97 ,  A61Q90/00

CPC分类号： A61K36/185 ,  A61K9/0019 ,  A61K9/08 ,  A61K9/2059 ,  A61K9/4866

摘要： Disclosed is an anti-obesity active extract obtained from a medicinal plant. More specifically, the extract obtained from lysimachiae foenum-graeci herba (which is a medicinal plant traditionally used in the East) can be used as an active ingredient for a raw material, a functional food, cosmetic, a crude drug, etc. for an obesity preventing or therapeutic agent because the extract can suppress the adipocyte differentiation and decrease the body weight and body fat in an obese animal, thereby suppressing fat accumulation (that is, a factor for obesity).

摘要翻译： 公开了从药用植物获得的抗肥胖活性提取物。 更具体地说，从益生草药（它是传统上用于东方的药用植物）获得的提取物可以用作原料，功能性食品，化妆品，生药等的活性成分，用于 因为提取物可以抑制肥胖动物的脂肪细胞分化和降低体重和体脂肪，从而抑制脂肪蓄积（即，肥胖的因素）。

公开(公告)号：US08168237B2

公开(公告)日：2012-05-01

申请号：US12095007

申请日：2008-05-08

申请人： Jong-Bae Seo ,  Sun-Mi Choi ,  Eun-Jung Choi ,  Sang-Wook Park ,  Eun-Wook Choi ,  Dong-Seung Seen ,  Tae-Gyu Lee

发明人： Jong-Bae Seo ,  Sun-Mi Choi ,  Eun-Jung Choi ,  Sang-Wook Park ,  Eun-Wook Choi ,  Dong-Seung Seen ,  Tae-Gyu Lee

IPC分类号： A61K36/00

CPC分类号： A61K36/185 ,  A61K9/0019 ,  A61K9/08 ,  A61K9/2059 ,  A61K9/4866

摘要： Disclosed is an anti-obesity active extract obtained from a medicinal plant. More specifically, the extract obtained from lysimachiae foenum-graeci herba (which is a medicinal plant traditionally used in the East) can be used as an active ingredient for a raw material, a functional food, cosmetic, a crude drug, etc. for an obesity preventing or therapeutic agent because the extract can suppress the adipocyte differentiation and decrease the body weight and body fat in an obese animal, thereby suppressing fat accumulation (that is, a factor for obesity).

摘要翻译： 公开了从药用植物获得的抗肥胖活性提取物。 更具体地说，从益生草药（其是东方传统使用的药用植物）获得的提取物可以用作原料，功能性食品，化妆品，生药等的有效成分，用于 因为提取物可以抑制肥胖动物的脂肪细胞分化和降低体重和体脂肪，从而抑制脂肪蓄积（即，肥胖的因素）。

公开(公告)号：US20100285156A1

公开(公告)日：2010-11-11

申请号：US12811056

申请日：2008-12-28

申请人： Jong-Bae Seo ,  Jae-Young Park ,  Sun-Mi Choi ,  Sang-Wook Park ,  Sung-Sik Choe ,  Eun-Wook Choi ,  Dong-Seung Seen ,  Tae-Gyu Lee

发明人： Jong-Bae Seo ,  Jae-Young Park ,  Sun-Mi Choi ,  Sang-Wook Park ,  Sung-Sik Choe ,  Eun-Wook Choi ,  Dong-Seung Seen ,  Tae-Gyu Lee

IPC分类号： A61K36/185 ,  A61P3/10 ,  A61P3/06 ,  A61P1/16 ,  A61P9/10

CPC分类号： A61K36/185

摘要： Disclosed are raw material, functional foods, and herb medicines for preventing and treating metabolic diseases, which comprise a Lysimachiae Foenum-Graeci Herba extract as an effective ingredient. The Lysimachiae Foenum-Graeci Herba extract have an activity of reducing blood glucose levels, triglycerides and cholesterol levels, AST and ALT levels, and fat in the liver, and thus can be effectively used as a hepatic protector and a remedy for preventing and treating various metabolic diseases including diabetes, high blood pressure, fatty liver, cardiovascular diseases, and hyperlipidemia.

摘要翻译： 公开了用于预防和治疗代谢疾病的原料，功能性食品和草药，其包括作为有效成分的赖e草提取物。 枸杞提取物具有降低血糖水平，甘油三酯和胆固醇水平，AST和ALT水平以及肝脏脂肪的活性，因此可以有效地用作肝脏保护剂和预防和治疗各种各样的药物 代谢疾病包括糖尿病，高血压，脂肪肝，心血管疾病和高脂血症。

公开(公告)号：US20050170428A1

公开(公告)日：2005-08-04

申请号：US10506925

申请日：2003-03-08

申请人： Dong-Seung Seen ,  Jac-Yong Park ,  Young-Shik Choi ,  Eun-Wook Choi ,  Ho-Sun Son ,  Neon-C. Jung ,  Anthony Kang ,  Ji-Ung Maeng ,  Kyung-Jin Kim ,  Jung-Hee Shin

发明人： Dong-Seung Seen ,  Jac-Yong Park ,  Young-Shik Choi ,  Eun-Wook Choi ,  Ho-Sun Son ,  Neon-C. Jung ,  Anthony Kang ,  Ji-Ung Maeng ,  Kyung-Jin Kim ,  Jung-Hee Shin

IPC分类号： C07K14/435 ,  G01N33/53 ,  A01K67/00

CPC分类号： C07K14/43595

摘要： Disclosed are mutated genes for green fluorescence proteins and enhanced inserted YFPs expressed therefrom. The mutant proteins not only maintain their fluorescence even at 37° C., but also exhibit about 20 times stronger fluorescence intensities in comparison to the conventional fluorescence proteins. Accordingly, the mutant fluorescence proteins of the present invention can be used as biosensors for detecting and analyzing the bioactivities of desired materials.

摘要翻译： 公开了绿色荧光蛋白和由其表达的增强的插入YFP的突变基因。 突变体蛋白质不仅在37℃下保持荧光，而且与常规荧光蛋白质相比也显示出强大的荧光强度的20倍。 因此，本发明的突变荧光蛋白可用作生物传感器，用于检测和分析所需材料的生物活性。

公开(公告)号：US07319001B2

公开(公告)日：2008-01-15

申请号：US10383838

申请日：2003-03-07

申请人： Ho-Sun Son ,  Do-Hui Kim ,  Neon-C Jung ,  Eun-Wook Choi ,  Dong-Seung Seen ,  Min-Sung Kim ,  Yong-Weon Yi ,  Kyung-Jin Kim

发明人： Ho-Sun Son ,  Do-Hui Kim ,  Neon-C Jung ,  Eun-Wook Choi ,  Dong-Seung Seen ,  Min-Sung Kim ,  Yong-Weon Yi ,  Kyung-Jin Kim

IPC分类号： C12N15/10 ,  C12N15/63 ,  C12N15/64 ,  C12N15/866 ,  C12N15/86 ,  A61K48/00

CPC分类号： C12N15/86 ,  C12N15/1027 ,  C12N2710/14143

摘要： Disclosed are the methods for producing recombinant viruses using site-specific recombination in vitro. In the present invention, circular viral genomic DNAs are digested with restriction enzymes to generate a linear form viral genomic DNAs flanked by site-specific recombination sites, and then are subjected to site-specific recombination with the desired genomic materials flanked by site-specific recombination sites in vitro. According to the present invention, since the site-specific recombination mixture can be applied to host cells without further procedures of selecting the desired recombinant viral genomic DNAs, it is possible to obtain numerous recombinant viruses rapidly at the same time. Thus, the present invention can be used as a high throughput system for generating and screening hundreds or thousands of recombinant viruses.

摘要翻译： 公开了使用体外位点特异性重组生产重组病毒的方法。 在本发明中，循环病毒基因组DNA用限制性酶消化以产生侧翼为位点特异性重组位点的线性形式的病毒基因组DNA，然后进行与所需基因组物质的位点特异性重组，所述基因组物质侧接于位点特异性重组 体外试验。 根据本发明，由于可以不用进一步选择所需的重组病毒基因组DNA的方式将位点特异性重组混合物施用于宿主细胞，所以可以同时快速获得多种重组病毒。 因此，本发明可以用作产生和筛选数百或数千种重组病毒的高通量系统。

公开(公告)号：US07528230B2

公开(公告)日：2009-05-05

申请号：US10506925

申请日：2003-03-08

申请人： Dong-Seung Seen ,  Jac-Yong Park ,  Young-Shik Choi ,  Eun-Wook Choi ,  Ho-Sun Son ,  Neon-C. Jung ,  Anthony D. Kang ,  Ji-Ung Maeng ,  Kyung-Jin Kim ,  Jung-Hee Shin

发明人： Dong-Seung Seen ,  Jac-Yong Park ,  Young-Shik Choi ,  Eun-Wook Choi ,  Ho-Sun Son ,  Neon-C. Jung ,  Anthony D. Kang ,  Ji-Ung Maeng ,  Kyung-Jin Kim ,  Jung-Hee Shin

IPC分类号： C07K14/00 ,  C07H21/02 ,  C12N5/00

CPC分类号： C07K14/43595

摘要： Disclosed are mutated genes for green fluorescence proteins and enhanced inserted YFPs expressed therefrom. The mutant proteins not only maintain their fluorescence even at 37° C., but also exhibit about 20 times stronger fluorescence intensities in comparison to the conventional fluorescence proteins. Accordingly, the mutant fluorescence proteins of the present invention can be used as biosensors for detecting and analyzing the bioactivities of desired materials.

摘要翻译： 公开了绿色荧光蛋白和由其表达的增强的插入YFP的突变基因。 突变体蛋白质不仅在37℃下保持荧光，而且与常规荧光蛋白质相比也显示出强大的荧光强度的20倍。 因此，本发明的突变荧光蛋白可用作生物传感器，用于检测和分析所需材料的生物活性。