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公开(公告)号：US20090311725A1
公开(公告)日：2009-12-17
申请号：US12299918
申请日：2007-05-15
CPC分类号： C12N15/85 , C07K16/00 , C07K16/065 , C07K2319/03
摘要： The current invention describes a nucleic acid comprising in a 5′ to 3′ direction a) a first nucleic acid encoding a heterologous polypeptide without an in frame stop codon, b) a second nucleic acid beginning with a 5′ splice donor site and terminated by a 3′ splice acceptor site comprising an in frame translational stop codon and a polyadenylation signal, and c) a nucleic acid encoding i) at least a fragment of a transmembrane domain, or ii) a signal peptide for a GPI-anchor.
摘要翻译： 本发明描述了包含在5'至3'方向的核酸a）编码不具有帧内终止密码子的异源多肽的第一核酸，b）以5'剪接供体位点开始并以 3''剪接受体位点，其包含内部翻译终止密码子和多聚腺苷酸化信号，以及c）编码i）至少跨膜结构域片段的核酸，或ii）GPI锚的信号肽。
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公开(公告)号：US08354516B2
公开(公告)日：2013-01-15
申请号：US12299918
申请日：2007-05-15
CPC分类号： C12N15/85 , C07K16/00 , C07K16/065 , C07K2319/03
摘要： The current invention describes a nucleic acid comprising in a 5′ to 3′ direction a) a first nucleic acid encoding a heterologous polypeptide without an in frame stop codon, b) a second nucleic acid beginning with a 5′ splice donor site and terminated by a 3′ splice acceptor site comprising an in frame translational stop codon and a polyadenylation signal, and c) a nucleic acid encoding i) at least a fragment of a transmembrane domain, or ii) a signal peptide for a GPI-anchor.
摘要翻译： 本发明描述了包含在5'至3'方向的核酸a）编码不具有帧内终止密码子的异源多肽的第一核酸，b）以5'剪接供体位点开始并以 3''剪接受体位点，其包含内部翻译终止密码子和多聚腺苷酸化信号，以及c）编码i）至少跨膜结构域片段的核酸，或ii）GPI锚的信号肽。
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公开(公告)号：US20100184144A1
公开(公告)日：2010-07-22
申请号：US12664401
申请日：2008-06-25
CPC分类号： C07K16/00 , C07K2317/21 , C07K2317/52 , C12N2830/42
摘要： The current invention comprises a nucleic acid encoding the amino acid sequence of the C-terminal part of the CH3-domain of an immunoglobulin of the class IgA or IgG, or the C-terminal part of the CH4-domain of an immunoglobulin of the class IgE or IgM, wherein the glycine-lysine-dipeptide comprised in the amino acid sequence of the C-terminal part of the CH3- or CH4-domain is encoded by the nucleic acid ggaaaa, or the nucleic acid ggcaaa, or the nucleic acid gggaaa, or the nucleic acid gggaag, or the nucleic acid ggcaag, or the nucleic acid ggaaag.
摘要翻译： 本发明包括编码IgA或IgG类免疫球蛋白的CH3结构域的C末端部分的氨基酸序列的核酸，或类别的免疫球蛋白的CH4结构域的C末端部分 IgE或IgM，其中包含在CH 3 - 或CH 4结构域的C-末端部分的氨基酸序列中的甘氨酸 - 赖氨酸二肽由核酸ggaaaa或核酸ggcaaa或核酸gggaaa ，或核酸gggaag，或核酸ggcaag，或核酸ggaaag。
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公开(公告)号：US08314225B2
公开(公告)日：2012-11-20
申请号：US12664401
申请日：2008-06-25
IPC分类号： C07H21/04
CPC分类号： C07K16/00 , C07K2317/21 , C07K2317/52 , C12N2830/42
摘要： The current invention comprises a nucleic acid encoding the amino acid sequence of the C-terminal part of the CH3-domain of an immunoglobulin of the class IgA or IgG, or the C-terminal part of the CH4-domain of an immunoglobulin of the class IgE or IgM, wherein the glycine-lysine-dipeptide comprised in the amino acid sequence of the C-terminal part of the CH3- or CH4-domain is encoded by the nucleic acid ggaaaa, or the nucleic acid ggcaaa, or the nucleic acid gggaaa, or the nucleic acid gggaag, or the nucleic acid ggcaag, or the nucleic acid ggaaag.
摘要翻译： 本发明包括编码IgA或IgG类免疫球蛋白的CH3结构域的C末端部分的氨基酸序列的核酸，或类别的免疫球蛋白的CH4结构域的C末端部分 IgE或IgM，其中包含在CH 3 - 或CH 4结构域的C-末端部分的氨基酸序列中的甘氨酸 - 赖氨酸二肽由核酸ggaaaa或核酸ggcaaa或核酸gggaaa ，或核酸gggaag，或核酸ggcaag，或核酸ggaaag。
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公开(公告)号：US20100221781A1
公开(公告)日：2010-09-02
申请号：US12681783
申请日：2008-10-13
申请人： Erhard Kopetzki , Ursula Schwarz
发明人： Erhard Kopetzki , Ursula Schwarz
CPC分类号： C12N15/85 , C07K16/18 , C07K16/28 , C07K16/2812 , C07K16/2854 , C07K16/2866 , C07K16/2896 , C07K2317/14 , C07K2317/21 , C07K2317/51 , C07K2317/515 , C12P21/00
摘要： The current invention reports a CHO-K1 cell, characterized in that said CHO-K 1 cell is derived from CHO-K1 cell deposited as ATCC CCL-61, grows in suspension, requires no glutamine, no insulin, and no growth factors in the cultivation medium for growth, whereby said CHO-K1 cell is not modified compared to the deposited CHO-K1 cell ATCC CCL-61 cell line by the introduction, deletion, or inactivation of a nucleic acid. Also reported are a method for obtaining said CHO-K1 cell and a method for the production of a heterologous polypeptide using such a CHO-K1 cell according to the invention.
摘要翻译： 本发明报道了一种CHO-K1细胞，其特征在于所述CHO-K1细胞衍生自作为ATCC CCL-61保藏的CHO-K1细胞，以悬浮液形式生长，不需要谷氨酰胺，不含胰岛素，且不含生长因子 培养培养基用于生长，由此通过引入，缺失或失活核酸，与保藏的CHO-K1细胞ATCC CCL-61细胞系相比，所述CHO-K1细胞不被修饰。 还报道了获得所述CHO-K1细胞的方法和使用本发明的CHO-K1细胞产生异源多肽的方法。
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6.发明授权Methods of inhibiting the binding of P-selectin to PSGL-1 with anti-P-selectin antibodies 有权用抗P-选择蛋白抗体抑制P-选择素与PSGL-1结合的方法公开(公告)号：US07824684B2
公开(公告)日：2010-11-02
申请号：US12768150
申请日：2010-04-27
申请人： Yvo Graus , Jacques Himber , Miranda Jansen-Molenaar , Dorothee Kling , Erhard Kopetzki , Paul Parren , Frank Rebers , Beat Steiner , Anne Stern , Pamela Strein , Kay-Gunnar Stubenrauch , Jan van de Winkel , Martine van Vugt
发明人： Yvo Graus , Jacques Himber , Miranda Jansen-Molenaar , Dorothee Kling , Erhard Kopetzki , Paul Parren , Frank Rebers , Beat Steiner , Anne Stern , Pamela Strein , Kay-Gunnar Stubenrauch , Jan van de Winkel , Martine van Vugt
IPC分类号： A61K39/395 , C07K16/28
CPC分类号： C07K16/2854 , C07K2317/21 , C07K2317/52 , C07K2317/56 , C07K2317/565 , C07K2317/76 , C07K2317/92 , Y10S435/81
摘要： This invention relates to anti-P-selectin antibodies and, in particular, to anti-P-selectin antibodies and variants thereof that contain an Fc part derived from human origin and do not bind complement factor C1q. These antibodies have new and inventive properties causing a benefit for a patient suffering from critical limb ischemia or peripheral arterial occlusive disease (CLI/PAOD).
摘要翻译： 本发明涉及抗P-选择素抗体，特别涉及含有源自人源的Fc部分并且不结合补体因子C1q的抗P-选择素抗体及其变体。 这些抗体具有新的和本发明的性质，为患有严重肢体缺血或外周动脉闭塞性疾病（CLI / PAOD）的患者带来益处。
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7.发明申请公开(公告)号：US20090275126A1
公开(公告)日：2009-11-05
申请号：US12504717
申请日：2009-07-17
申请人： Yvo Graus , Erhard Kopetzki , Klaus-Peter Kuenkele , Olaf Mundigl , Paul Parren , Frank Rebers , Ralf Schumacher , Jan van de Winkel , Martine Vriesema-van Vugt
发明人： Yvo Graus , Erhard Kopetzki , Klaus-Peter Kuenkele , Olaf Mundigl , Paul Parren , Frank Rebers , Ralf Schumacher , Jan van de Winkel , Martine Vriesema-van Vugt
CPC分类号： C07K16/2863 , A61K2039/505 , C07K16/2896 , C07K2317/73
摘要： Antibodies against insulin like growth factor I receptor (IGF-IR), methods for their production, pharmaceutical compositions containing said antibodies, and uses for such antibodies are disclosed. Such antibodies are implicated in antitumor therapy.
摘要翻译： 公开了针对胰岛素样生长因子I受体（IGF-1R）的抗体，其制备方法，含有所述抗体的药物组合物及其用途。 这些抗体涉及抗肿瘤治疗。
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8.发明授权公开(公告)号：US07378503B2
公开(公告)日：2008-05-27
申请号：US10815449
申请日：2004-04-01
申请人： Yvo Graus , Erhard Kopetzki , Klaus-Peter Kuenkele , Olaf Mundigl , Paul Parren , Frank Rebers , Ralf Schumacher , Jan van de Winkel , Martine Vriesema-van Vugt
发明人： Yvo Graus , Erhard Kopetzki , Klaus-Peter Kuenkele , Olaf Mundigl , Paul Parren , Frank Rebers , Ralf Schumacher , Jan van de Winkel , Martine Vriesema-van Vugt
CPC分类号： C07K16/2863 , A61K2039/505 , C07K2317/21 , C07K2317/52 , C07K2317/56 , C07K2317/565
摘要： Antibodies which bind to IGF-IR and inhibit the binding of IGF-I and IGF-II to IGF-IR are characterized. These antibodies are implicated in anti-tumor therapy.
摘要翻译： 表征与IGF-1R结合并抑制IGF-I和IGF-II与IGF-1R结合的抗体。 这些抗体涉及抗肿瘤治疗。
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公开(公告)号：US20070117185A1
公开(公告)日：2007-05-24
申请号：US11583573
申请日：2006-10-19
申请人： Erhard Kopetzki
发明人： Erhard Kopetzki
CPC分类号： C07K14/005 , A61K38/162 , C07K16/00 , C07K16/2863 , C07K2317/50 , C07K2319/00 , C12N2740/16122
摘要： A method for the recombinant production of a heterologous polypeptide in a eukaryotic host cell is described. The host cell comprises an expression plasmid, whereby the expression plasmid comprises in a 5′ to 3′ direction a) a promoter, b) a nucleic acid encoding a first polypeptide, whose amino acid sequence is selected from Table 1 depending on the first two amino acids of the second polypeptide, c) a nucleic acid encoding a second polypeptide comprising a nucleic acid encoding a heterologous polypeptide, a nucleic acid encoding a linker, and a nucleic acid encoding an immunoglobulin fragment, and d) a 3′ untranslated region comprising a polyadenylation signal. Further a plasmid and a kit are described.
摘要翻译： 描述了在真核宿主细胞中重组产生异源多肽的方法。 宿主细胞包含表达质粒，其中表达质粒包含5'至3'方向a）启动子，b）编码第一多肽的核酸，其氨基酸序列选自表1，取决于前两个 第二多肽的氨基酸，c）编码包含编码异源多肽的核酸，编码接头的核酸和编码免疫球蛋白片段的核酸的第二多肽的核酸，以及d）3'非翻译区，其包含 多聚腺苷酸化信号。 此外，还描述了质粒和试剂盒。
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公开(公告)号：US07034119B2
公开(公告)日：2006-04-25
申请号：US10866567
申请日：2004-06-12
IPC分类号： C07K1/00
CPC分类号： C12P21/02
摘要： A method for the recombinant production of a polypeptide by expressing a nucleic acid encoding said polypeptide in a microbial host cell, forming in the cytoplasm of said host cell inclusion bodies containing said polypeptide, and isolating, solubilizing and naturing said polypeptide, characterized in that after fermentation the host cell or the host cell content is incubated at a temperature of 40° C. or higher for at least 10 minutes and subsequently insoluble polypeptide is isolated from the host cell, this method providing an improved yield in inclusion bodies containing the desired polypeptide.
摘要翻译： 一种通过在微生物宿主细胞中表达编码所述多肽的核酸重组产生多肽的方法，在包含所述多肽的所述宿主细胞包涵体的细胞质中形成，并分离，增溶和变性所述多肽，其特征在于， 将宿主细胞或宿主细胞含量发酵在40℃或更高的温度下孵育至少10分钟，随后从宿主细胞中分离不溶性多肽，该方法在含有所需多肽的包涵体中提供改善的产量 。
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