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    LC-MS SEPARATION AND DETECTION OF VITAMIN D METABOLITES
    3.
    发明申请
    LC-MS SEPARATION AND DETECTION OF VITAMIN D METABOLITES 审中-公开
    维生素D代谢物的LC-MS分离和检测

    公开(公告)号：US20140147878A1

    公开(公告)日：2014-05-29

    申请号：US13881843

    申请日：2011-10-28

    申请人： Joseph L. Herman ,  Dayana Argoti

    发明人： Joseph L. Herman ,  Dayana Argoti

    IPC分类号： G01N33/82

    CPC分类号： G01N33/82 ,  G01N2560/00 ,  Y10T436/203332

    摘要： Systems, kits, and methods for quantitation of metabolites of vitamin D by liquid chromatography-mass spectrometry (LC-MS). The systems, kits, and methods described herein stabilize and/or promote the formation of the protonated molecular ion ([M+H]+) for the vitamin D metabolites in the ionization source (e.g., electrospray ionization (“ESI”)). Formation of the molecular ion does not involve loss of a water molecule from the parent molecule. Subsequent fragmentation of the [M+H]+ ion yields product ions that are specific to each molecular ion. The systems, kits, and methods described herein provide for no compromise in specificity and provide for a significant increase in sensitivity relative to previously described methods.

    摘要翻译： 通过液相色谱 - 质谱（LC-MS）定量维生素D代谢物的系统，试剂盒和方法。 本文所述的系统，试剂盒和方法稳定和/或促进电离源（例如，电喷雾离子化（“ESI”））中维生素D代谢物的质子化分子离子（[M + H] +）的形成）。 分子离子的形成不涉及从母体分子损失水分子。 随后的[M + H] +离子的断裂产生对每个分子离子特异的产物离子。 本文所述的系统，试剂盒和方法提供了特异性的妥协，并且提供了相对于先前描述的方法显着增加的灵敏度。

    LC-MS separation and detection of vitamin D metabolites
    4.
    发明授权
    LC-MS separation and detection of vitamin D metabolites 有权
    LC-MS分离和检测维生素D代谢物

    公开(公告)号：US09063159B2

    公开(公告)日：2015-06-23

    申请号：US13881843

    申请日：2011-10-28

    申请人： Joseph L. Herman ,  Dayana Argoti

    发明人： Joseph L. Herman ,  Dayana Argoti

    IPC分类号： G01N33/00 ,  G01N33/82

    CPC分类号： G01N33/82 ,  G01N2560/00 ,  Y10T436/203332

    摘要： Systems, kits, and methods for quantitation of metabolites of vitamin D by liquid chromatography-mass spectrometry (LC-MS). The systems, kits, and methods described herein stabilize and/or promote the formation of the protonated molecular ion ([M+H]+) for the vitamin D metabolites in the ionization source (e.g., electrospray ionization (“ESI”)). Formation of the molecular ion does not involve loss of a water molecule from the parent molecule. Subsequent fragmentation of the [M+H]+ ion yields product ions that are specific to each molecular ion. The systems, kits, and methods described herein provide for no compromise in specificity and provide for a significant increase in sensitivity relative to previously described methods.

    摘要翻译： 通过液相色谱 - 质谱（LC-MS）定量维生素D代谢物的系统，试剂盒和方法。 本文所述的系统，试剂盒和方法稳定和/或促进电离源（例如，电喷雾离子化（“ESI”））中维生素D代谢物的质子化分子离子（[M + H] +）的形成）。 分子离子的形成不涉及从母体分子损失水分子。 随后的[M + H] +离子的断裂产生对每个分子离子特异的产物离子。 本文所述的系统，试剂盒和方法提供了特异性的妥协，并且提供了相对于先前描述的方法显着增加的灵敏度。

    LC-MS CONFIGURATION FOR PURIFICATION AND DETECTION OF ANALYTES HAVING A BROAD RANGE OF HYDROPHOBICITES
    5.
    发明申请
    LC-MS CONFIGURATION FOR PURIFICATION AND DETECTION OF ANALYTES HAVING A BROAD RANGE OF HYDROPHOBICITES 审中-公开
    用于净化和检测具有广泛的疏水性分析物的分析仪的LC-MS配置

    公开(公告)号：US20140047906A1

    公开(公告)日：2014-02-20

    申请号：US13881848

    申请日：2011-10-28

    申请人： Joseph L. Herman ,  Robert Dewitte ,  Dayana Argoti

    发明人： Joseph L. Herman ,  Robert Dewitte ,  Dayana Argoti

    IPC分类号： G01N30/72

    CPC分类号： G01N30/7233 ,  G01N30/32 ,  G01N30/34 ,  G01N30/88 ,  G01N33/6848 ,  G01N33/82 ,  G01N33/9493 ,  G01N2030/8813 ,  G01N2430/00

    摘要： Systems, apparatuses, kits, and methods for purification and analysis of analytes having a broad range of hydrophobicities by liquid chromatography-mass spectrometry (LC-MS). Using one set of liquid chromatography columns, one set of mobile phase buffers, and, optionally, a single ionization method (e.g., electrospray ionization), a wide range of analytes can be purified and analyzed on a liquid chromatography-mass spectrometry (LC-MS) system. LC-MS purification and analysis of analytes having a broad range of partition coefficients is accomplished by selecting LC run parameters and MS system parameters that are particular to different classes of analytes without having to make column or buffer changes or any other hardware configuration changes to the LC-MS system. The methods, systems, and kits described herein provide for substantially increased speed/throughput and ease of use for a wide range analytes with essentially no compromise in specificity for individual analytes relative to previously described methods.

    摘要翻译： 通过液相色谱 - 质谱（LC-MS）纯化和分析具有广泛疏水性的分析物的系统，设备，试剂盒和方法。 使用一套液相色谱柱，一组流动相缓冲液，以及任选的单一离子化方法（如电喷雾离子化），可以在液相色谱 - 质谱（LC- MS）系统。 通过选择不同类别分析物特有的LC运行参数和MS系统参数，而不必对列或缓冲区进行更改或对其进行任何其他硬件配置更改，从而实现具有宽范围分配系数的分析物的LC-MS纯化和分析。 LC-MS系统。 本文描述的方法，系统和试剂盒提供了对于宽范围分析物的显着提高的速度/通量和易用性，其相对于先前描述的方法基本上不损害针对各个分析物的特异性。

    System for analysis of phase-I and phase-II metabolites and parent compounds without hydrolysis
    6.
    发明授权
    System for analysis of phase-I and phase-II metabolites and parent compounds without hydrolysis 有权
    用于分析I期和II期代谢物和母体化合物而不进行水解的系统

    公开(公告)号：US09404900B2

    公开(公告)日：2016-08-02

    申请号：US13724316

    申请日：2012-12-21

    申请人： Joseph Herman ,  Sarah J. Fair ,  Dayana Argoti

    发明人： Joseph Herman ,  Sarah J. Fair ,  Dayana Argoti

    IPC分类号： G01N30/02 ,  G01N30/08 ,  G01N30/14 ,  G01N30/46

    CPC分类号： G01N30/462 ,  G01N30/02 ,  G01N30/08 ,  G01N30/14 ,  G01N30/468 ,  G01N2030/027 ,  G01N2030/143 ,  G01N2030/628

    摘要： An apparatus comprises: a first chromatographic column fluidically coupled to a source of sample and a source of a first chromatographic mobile phase solvent; a second chromatographic column fluidically coupled to the first column; a source of a second mobile phase solvent fluidically coupled between the first and second columns; and a detector, the first chromatographic column being configurable to receive, in a trapping step, the first solvent and sample and to retain a first portion of a plurality of analytes therein and to pass a second portion of the plurality of analytes therethrough, the second chromatographic column being configurable, in the trapping step, to receive the second portion of the plurality of analytes and the first and second solvents and to retain the second portion of the plurality of analytes therein, the detector being arranged to receive the second and first portions in respective first and second elution steps.

    摘要翻译： 一种装置包括：流体耦合到样品源和第一色谱流动相溶剂源的第一色谱柱; 流体耦合到第一柱的第二色谱柱; 流体耦合在第一和第二列之间的第二流动相溶剂的源; 和检测器，所述第一色谱柱可配置为在捕获步骤中接收所述第一溶剂和样品并将多个分析物的第一部分保留在其中并使所述多个分析物的第二部分通过其中，所述第二色谱柱 色谱柱是可配置的，在捕获步骤中，接收多个分析物的第二部分以及第一和第二溶剂并将多个分析物的第二部分保持在其中，检测器被布置成接收第二部分和第二部分 在相应的第一和第二洗脱步骤中。