公开(公告)号：US20180196019A1

公开(公告)日：2018-07-12

申请号：US15850146

申请日：2017-12-21

申请人： Waters Technologies Corporation

发明人： Michael O. Fogwill ,  Isabelle François

IPC分类号： G01N30/26 ,  G01N30/60

CPC分类号： G01N30/26 ,  G01N30/20 ,  G01N30/34 ,  G01N30/462 ,  G01N30/463 ,  G01N30/465 ,  G01N30/60 ,  B01D15/40

摘要： A method of performing a chromatographic separation includes modulating a portion of a flowstream to a trap column to retain at least one analyte from the flowstream on the trap column. A flow of a modifier is provided through the trap column to generate an elution comprising the at least one analyte. A flow of a compressible fluid-based chromatography (CFC) mobile phase or CFC solvent is merged with the elution from the trap column to generate a diluted elution. Carbon dioxide may be used as the CFC solvent or as a component of the CFC mobile phase. The diluted elution is provided to a CFC column where at least one analyte is focused at a head of the CFC column. Examples of a flowstream that may be used include an eluent from a chromatography column or a fluid flow from an extraction system.

公开(公告)号：US20170029802A1

公开(公告)日：2017-02-02

申请号：US15222800

申请日：2016-07-28

申请人： FloDesign Sonics, Inc.

发明人： Bart Lipkens ,  Rudolf Gilmanshin ,  Walter M. Presz ,  Louis Masi ,  Thomas J. Kennedy, III

IPC分类号： C12N13/00 ,  G01N30/72 ,  G01N30/46 ,  G01N30/96 ,  C12M1/42 ,  C12M1/00

CPC分类号： C12N13/00 ,  A61K2039/545 ,  C07K16/2851 ,  C12M35/04 ,  C12M47/02 ,  G01N30/462 ,  G01N30/72 ,  G01N30/96 ,  G01N33/54313 ,  G01N2030/027

摘要： Methods and systems for separating a first biomaterial from a second biomaterial can use functionalized material retained in a liquid-filled chamber at locales within an acoustic standing wave field. A culture suspension containing the first biomaterial and the second biomaterial flows into the liquid-filled chamber and at least portions of the first biomaterial with features complementary to the functionalized material becomes bound to the functionalized material while other portions of the culture suspension containing the second material pass through the chamber. The portion of the first biomaterial bound to the functionalized material is subsequently released from the liquid filled chamber.

摘要翻译： 用于从第二生物材料分离第一生物材料的方法和系统可以使用保持在充满液体的腔室中的功能化材料在声驻波场内的区域。 包含第一生物材料和第二生物材料的培养悬浮液流入液体填充的室，并且具有与官能化材料互补的特征的第一生物材料的至少部分变得与官能化材料结合，而含有第二材料的培养悬浮液的其它部分 通过房间。 与功能化材料结合的第一生物材料的部分随后从充满液体的室中释放。

公开(公告)号：US20120131987A1

公开(公告)日：2012-05-31

申请号：US13133856

申请日：2009-12-09

申请人： Udo Gellert ,  Frank Probst

发明人： Udo Gellert ,  Frank Probst

IPC分类号： G01N30/04

CPC分类号： G01N30/462 ,  G01N30/34 ,  G01N30/461 ,  G01N30/463 ,  G01N33/28 ,  G01N2030/382 ,  G01N2030/402

摘要： A method for analyzing a gas mixture through gas chromatography, wherein a sample of the gas mixture is fed to a dosing volume using a metering valve in a first valve position and, in a second valve position the sample of the gas mixture is fed from the dosing volume through a separating device by a carrier gas. A gas component of interest, arriving at the separating device and separated from the sample, is detected by a detecting device. Part of the separating device and the metering valve are flushed with another carrier gas after the gas component of interest has passed through a part of the separating device to facilitate a precise gas chromatography analysis using minimal technical effort. Another gas sample is then fed from the dosing volume to the separating device using the other carrier gas.

摘要翻译： 一种用于通过气相色谱法分析气体混合物的方法，其中使用第一阀位置中的计量阀将气体混合物的样品供给到计量体积，并且在第二阀位置中，将气体混合物的样品从 通过载气通过分离装置计量体积。 通过检测装置检测到达分离装置并与样品分离的感兴趣的气体成分。 在感兴趣的气体成分已经通过分离装置的一部分之后，分离装置和计量阀的一部分用另一个载气冲洗，以便利用最少的技术努力进行精确的气相色谱分析。 然后使用另一种载气将另外的气体样品从计量体积输送到分离装置。

公开(公告)号：US20110212536A1

公开(公告)日：2011-09-01

申请号：US12742364

申请日：2008-10-28

申请人： Michael Krummen ,  Johannes Schwieters

发明人： Michael Krummen ,  Johannes Schwieters

IPC分类号： G01N30/02 ,  B01D59/44 ,  G01N21/59

CPC分类号： G01N30/462 ,  B01D59/44 ,  G01N30/466 ,  G01N30/7273 ,  G01N30/84 ,  G01N2001/4016 ,  G01N2001/4027 ,  G01N2030/8411 ,  G01N2030/8494 ,  G01N2030/8868

摘要： A process and to an apparatus for isotope ratio analysis having the steps of: performing an LC process and thus providing a first eluate which comprises at least one first liquid carrier fluid and one or more analytes, collecting a portion of interest from the eluate, processing the eluate portion of interest by combining with a second liquid carrier fluid and removing the first carrier fluid to form a processed eluate portion, processing the processed eluate portion to form one or more gaseous conversion products of the analytes, and supplying the gaseous conversion products with gaseous carrier fluid to an isotope analyzer and determining the isotope ratios.

摘要翻译： 一种用于同位素比率分析的方法和装置，其具有以下步骤：执行LC过程，从而提供第一洗脱液，其包含至少一种第一液体载体流体和一种或多种分析物，从所述洗脱物中收集感兴趣的部分，处理 通过与第二液体载体流体结合并除去第一载体流体以形成经处理的洗脱液部分，处理所述经处理的洗脱液部分以形成所述分析物的一种或多种气态转化产物，并将所述气态转化产物供应至 气态载体流体到同位素分析仪，并确定同位素比率。

公开(公告)号：US20090000358A1

公开(公告)日：2009-01-01

申请号：US11579476

申请日：2004-05-04

申请人： Hendrik Kerkdijk

发明人： Hendrik Kerkdijk

IPC分类号： G01N30/84

CPC分类号： G01N30/461 ,  G01N30/34 ,  G01N30/462 ,  G01N2030/085

摘要： The present invention relates to a method for analysing a fluid, comprising applying the fluid to a pre-separation module and subjecting the fluid to a chromatographic pre-separation, taking a sample from said zone; and subjecting said sample to an analytical separation. The invention further relates to an apparatus for analysing a fluid, suitable for carrying out a method according to the invention.

摘要翻译： 本发明涉及一种用于分析流体的方法，包括将流体施加到预分离模块并使流体进行色谱预分离，从所述区域取样品; 并对所述样品进行分析分离。 本发明还涉及一种用于分析适于实施根据本发明的方法的流体的装置。

公开(公告)号：US20070295664A1

公开(公告)日：2007-12-27

申请号：US11820445

申请日：2007-06-19

申请人： Bernd Glatz ,  Karsten Kraiczek

发明人： Bernd Glatz ,  Karsten Kraiczek

IPC分类号： B01D15/08

CPC分类号： G01N30/462 ,  G01N30/463 ,  G01N30/465 ,  G01N30/468 ,  G01N2030/347

摘要： A focusing unit for a fluidic system adapted for processing a mobile phase containing a fluidic sample, the fluidic system having a first processing element and a second processing element each for interacting with the mobile phase, wherein the mobile phase is to be conducted through the first and second processing elements, wherein the focusing unit is adapted for being coupled to an inlet of the second processing element and is adapted for modifying an elution strength of the mobile phase in order to spatially focus at least a portion of the fluidic sample in a region close to the inlet of the second processing element.

摘要翻译： 一种用于流体系统的聚焦单元，适用于处理含有流体样品的流动相，所述流体系统具有第一处理元件和第二处理元件，每个处理元件和第二处理元件各自与流动相相互作用，其中流动相通过第一 和第二处理元件，其中所述聚焦单元适于耦合到所述第二处理元件的入口，并且适于改变所述流动相的洗脱强度，以便将所述流体样品的至少一部分空间聚焦在区域 靠近第二处理元件的入口。

公开(公告)号：US20040124128A1

公开(公告)日：2004-07-01

申请号：US10725387

申请日：2003-12-03

发明人： Yosuke Iwata

IPC分类号： B01D015/08

CPC分类号： G01N30/462 ,  G01N30/461 ,  G01N2030/8411 ,  G01N2030/121

摘要： In a trap flow path, two diluents are supplied by solvent pumps that can respectively determine the flow rates independently. One of the diluents is allowed to pass through a fraction loop so as to direct a fractioned component(s) held in the fraction loop to a trap column together with a mobile phase. The other diluent is allowed to join with a flow path that has passed through the fraction loop on the downstream side of the fraction loop, and flows to the trap column while diluting the mobile phase from the flow path. In the trap column, the sample component(s) is condensed while being trapped therein.

摘要翻译： 在陷阱流动路径中，两个稀释剂由溶剂泵提供，可分别独立确定流量。 允许一种稀释剂通过级分回路，以将保留在级分循环中的分馏组分与流动相一起引导到捕集柱。 允许另一种稀释剂与已经通过馏分回路的下游侧的馏分回路的流动路径连接，并且在从流动路径稀释流动相的同时流入捕集塔。 在捕集塔中，样品组分在被捕获时被冷凝。

公开(公告)号：US5522988A

公开(公告)日：1996-06-04

申请号：US318481

申请日：1994-10-05

申请人： Hernan J. Cortes ,  Curtis D. Pfeiffer ,  Bruce E. Richter

发明人： Hernan J. Cortes ,  Curtis D. Pfeiffer ,  Bruce E. Richter

IPC分类号： G01N30/02 ,  G01N30/16 ,  G01N30/46 ,  B01D15/08

CPC分类号： G01N30/465 ,  G01N2030/025 ,  G01N2030/165 ,  G01N30/462

摘要： Apparatus and method for on-line coupled liquid chromatography and gas chromatography analysis. The system includes a high-volume, liquid vaporizing chamber, or interface capillary tube which allows injection of a substantial volume of sample-carrying eluent and concentrates bands, obviating adverse effects on the resolution or peak shape of the components of interest. The vaporizing chamber is interposed between a packed or wall-coated open tubular capillary liquid chromatographic column and a capillary gas chromatographic column. The capillary liquid chromatographic column permits the use of small volumes of eluent and reduced flow rates.

摘要翻译： 在线耦合液相色谱和气相色谱分析的仪器和方法。 该系统包括大容量的液体蒸发室或接口毛细管，其允许注入大量的样品携带洗脱液和浓缩物带，消除对感兴趣的组分的分辨率或峰形的不利影响。 蒸发室介于填充或壁涂开放的管状毛细管液相色谱柱和毛细管气相色谱柱之间。 毛细管液相色谱柱允许使用少量洗脱液和降低流速。

公开(公告)号：US5376277A

公开(公告)日：1994-12-27

申请号：US68486

申请日：1993-05-28

申请人： Hernan J. Cortes ,  Curtis D. Pfeiffer ,  Steven J. Martin ,  Charles G. Smith ,  Gary L. Jewett

发明人： Hernan J. Cortes ,  Curtis D. Pfeiffer ,  Steven J. Martin ,  Charles G. Smith ,  Gary L. Jewett

IPC分类号： G01N30/02 ,  G01N30/12 ,  G01N30/46 ,  G01N30/84 ,  G01N31/12 ,  G01N33/44 ,  B01D15/08

CPC分类号： G01N30/463 ,  G01N30/12 ,  G01N30/84 ,  G01N31/12 ,  G01N2030/025 ,  G01N2030/125 ,  G01N2030/8405 ,  G01N30/02 ,  G01N30/462 ,  G01N33/44

摘要： An on-line multidimensional system which includes a liquid chromatograph having an on-line connection to a pyrolysis probe, which in turn has on-line connection to a gas chromatograph. Preferred applications use a size-exclusion chromatograph coupled to a pyrolysis probe coupled to a gas chromatograph to simultaneously produce composition as a function of molecular weight/size information for polymeric materials.

摘要翻译： 一种在线多维系统，其包括具有与热解探针的在线连接的液相色谱仪，其又与气相色谱仪在线连接。 优选的应用使用与耦合到气相色谱仪的热解探针耦合的尺寸排阻色谱，以同时产生作为聚合物材料的分子量/尺寸信息的函数的组成。

公开(公告)号：US5234599A

公开(公告)日：1993-08-10

申请号：US837460

申请日：1992-02-14

申请人： Hernan J. Cortes ,  Robert M. Campbell ,  R. Paul Himes ,  Curtis D. Pfeffer

发明人： Hernan J. Cortes ,  Robert M. Campbell ,  R. Paul Himes ,  Curtis D. Pfeffer

IPC分类号： G01N30/02 ,  G01N30/06 ,  G01N30/12 ,  G01N30/38 ,  G01N30/46

CPC分类号： G01N30/463 ,  G01N2030/062 ,  G01N2030/126 ,  G01N30/02 ,  G01N30/38 ,  G01N30/462

摘要： An on-line multidimensional chromatographic system and method which couples a liquid chromatograph to a supercritical fluid chromatograph. The system includes an inlet column for depositing a fraction of interest eluting from the liquid chromatograph, an interface interposed between the inlet column and the supercritical fluid chromatograph for receiving and trapping an analyte from the deposited fraction on an impactor surface, and a valve arrangement for controlling fluid flow throughout the system. After the fraction of interest has been deposited in the inlet column, the liquid chromatograph solvent is removed from the inlet column, and then the deposited fraction is extracted from the inlet column in a supercritical fluid stream. The supercritical fluid stream is directed into the interface, where the analyte is trapped while the supercritical fluid is decompressed and vented. A subsequent supercritical fluid stream is then used to introduce the trapped analyte directly into the analytical column of the supercritical fluid chromatograph. The system and method can also be used to introduce samples contained in large volumes of solvent without the prior liquid chromatographic separation.

摘要翻译： 一种将液相色谱仪与超临界流体色谱仪相结合的在线多维色谱系统和方法。 该系统包括用于从液相色谱仪洗脱出一部分沉积物的入口柱，介于入口柱和超临界流体色谱仪之间的界面，用于接收和捕获来自沉积部分的分析物在冲击器表面上，以及阀装置 控制流经整个系统的流体。 感兴趣的部分已经沉积在入口塔中后，液体色谱溶剂从入口塔中除去，然后以超临界流体流从入口塔提取沉积的馏分。 将超临界流体流引入界面，在该界面中分析物被捕获，同时超临界流体被减压和排气。 然后使用随后的超临界流体流将捕获的分析物直接引入超临界流体色谱仪的分析柱中。 该系统和方法也可以用于在没有先前的液相色谱分离的情况下引入包含在大量溶剂中的样品。