公开(公告)号：US09625429B2

公开(公告)日：2017-04-18

申请号：US13881848

申请日：2011-10-28

申请人： Joseph L. Herman ,  Robert Dewitte ,  Dayana Argoti

发明人： Joseph L. Herman ,  Robert Dewitte ,  Dayana Argoti

IPC分类号： G01N30/34 ,  G01N30/32 ,  G01N30/88 ,  G01N30/72 ,  G01N33/68

CPC分类号： G01N30/7233 ,  G01N30/32 ,  G01N30/34 ,  G01N30/88 ,  G01N33/6848 ,  G01N33/82 ,  G01N33/9493 ,  G01N2030/8813 ,  G01N2430/00

摘要： Systems, apparatuses, kits, and methods for purification and analysis of analytes having a broad range of hydrophobicities by liquid chromatography-mass spectrometry (LC-MS). Using one set of liquid chromatography columns, one set of mobile phase buffers, and, optionally, a single ionization method (e.g., electrospray ionization), a wide range of analytes can be purified and analyzed on a liquid chromatography-mass spectrometry (LC-MS) system. LC-MS purification and analysis of analytes having a broad range of partition coefficients is accomplished by selecting LC run parameters and MS system parameters that are particular to different classes of analytes without having to make column or buffer changes or any other hardware configuration changes to the LC-MS system. The methods, systems, and kits described herein provide for substantially increased speed/throughput and ease of use for a wide range analytes with essentially no compromise in specificity for individual analytes relative to previously described methods.

公开(公告)号：US09000360B2

公开(公告)日：2015-04-07

申请号：US13882399

申请日：2011-10-28

申请人： Robert DeWitte ,  Juhani Siidorov ,  Vesa Nuotio ,  Jukka Saukkonen ,  John Edward Brann, III ,  Terry N. Olney

发明人： Robert DeWitte ,  Juhani Siidorov ,  Vesa Nuotio ,  Jukka Saukkonen ,  John Edward Brann, III ,  Terry N. Olney

IPC分类号： H01J49/26 ,  H01J49/10 ,  H01J49/00 ,  H01J49/04 ,  G01N30/72

CPC分类号： G01N30/7233 ,  G01N27/62 ,  G01N30/06 ,  G01N30/16 ,  G01N30/72 ,  G01N30/8658 ,  G01N30/88 ,  G01N35/00871 ,  G01N35/0092 ,  G01N35/026 ,  G01N35/08 ,  G01N2030/027 ,  G01N2030/067 ,  G01N2030/628 ,  G01N2030/8804 ,  G01N2030/8813 ,  G06F19/00 ,  H01J49/00 ,  H01J49/0031 ,  H01J49/0409 ,  H01J49/0413 ,  H01J49/0431 ,  H01J49/10 ,  Y10T436/24

摘要： A sample preparation and analysis system (10). The system (10) includes a housing (16) with a sample preparation station (12) and a sample analysis station (14) positioned within the housing (16). The sample analysis station (14) is spaced away from the sample preparation station (12). A transport assembly (50) is configured to move at least one sample within the housing (16) and between the sample preparation station (12) and the sample analysis station (14).

摘要翻译： 样品制备和分析系统（10）。 系统（10）包括具有样品制备站（12）和位于壳体（16）内的样品分析站（14）的壳体（16）。 样品分析站（14）与样品制备站（12）间隔开。 运输组件（50）被配置为移动壳体（16）内和样品制备站（12）与样品分析站（14）之间的至少一个样品。

公开(公告)号：US20130295597A1

公开(公告)日：2013-11-07

申请号：US13882393

申请日：2011-10-28

申请人： Robert DeWitte ,  Juhani Siidorov ,  Vesa Nuotio ,  Raimo Salminen ,  Jarmo Vehkomaki ,  Jukka Saukkonen ,  Bill Ostman ,  Joseph M. Senteno ,  John Edward Brann, III ,  Joseph L. Herman ,  Jeffrey A. Zonderman ,  Terry N. Olney

发明人： Robert DeWitte ,  Juhani Siidorov ,  Vesa Nuotio ,  Raimo Salminen ,  Jarmo Vehkomaki ,  Jukka Saukkonen ,  Bill Ostman ,  Joseph M. Senteno ,  John Edward Brann, III ,  Joseph L. Herman ,  Jeffrey A. Zonderman ,  Terry N. Olney

IPC分类号： G01N30/72

CPC分类号： G01N30/7233 ,  G01N27/62 ,  G01N30/06 ,  G01N30/16 ,  G01N30/72 ,  G01N30/8658 ,  G01N30/88 ,  G01N35/00871 ,  G01N35/0092 ,  G01N35/026 ,  G01N35/08 ,  G01N2030/027 ,  G01N2030/067 ,  G01N2030/628 ,  G01N2030/8804 ,  G01N2030/8813 ,  G06F19/00 ,  H01J49/00 ,  H01J49/0031 ,  H01J49/0409 ,  H01J49/0413 ,  H01J49/0431 ,  H01J49/10 ,  Y10T436/24

摘要： A sample preparation and analysis system. The system 10 includes a sample preparation system 12 and a sample analysis system 14. The sample preparation system 12 prepares samples in accordance with an assay that is selected from a database containing a plurality of unique assays. The sample analysis system 14 includes an analyzer 110 that is dynamically reconfigurable based on the selected assay so as to analyze the prepared sample in accordance with that selected assay. A data communication link 115a communicates data from the sample preparation system 12 to the sample analysis system 14 to reconfigure the analyzer 110 in accordance with the selected assay.

摘要翻译： 样品制备和分析系统。 系统10包括样品制备系统12和样品分析系统14.样品制备系统12根据从包含多个独特测定的数据库中选择的测定准备样品。 样本分析系统14包括基于所选择的测定法动态地可重新配置的分析器110，以便根据所选择的测定来分析所制备的样品。 数据通信链路115a将来自样品制备系统12的数据传送到样品分析系统14，以根据所选择的测定重新配置分析器110。

公开(公告)号：US20130288355A1

公开(公告)日：2013-10-31

申请号：US13882399

申请日：2011-10-28

申请人： Robert DeWitte ,  Juhani Siidorov ,  Vesa Nuotio ,  Jukka Saukkonen ,  John Edward Brann, III ,  Terry N. Olney

发明人： Robert DeWitte ,  Juhani Siidorov ,  Vesa Nuotio ,  Jukka Saukkonen ,  John Edward Brann, III ,  Terry N. Olney

IPC分类号： G01N27/62

CPC分类号： G01N30/7233 ,  G01N27/62 ,  G01N30/06 ,  G01N30/16 ,  G01N30/72 ,  G01N30/8658 ,  G01N30/88 ,  G01N35/00871 ,  G01N35/0092 ,  G01N35/026 ,  G01N35/08 ,  G01N2030/027 ,  G01N2030/067 ,  G01N2030/628 ,  G01N2030/8804 ,  G01N2030/8813 ,  G06F19/00 ,  H01J49/00 ,  H01J49/0031 ,  H01J49/0409 ,  H01J49/0413 ,  H01J49/0431 ,  H01J49/10 ,  Y10T436/24

摘要： A sample preparation and analysis system (10). The system (10) includes a housing (16) with a sample preparation station (12) and a sample analysis station (14) positioned within the housing (16). The sample analysis station (14) is spaced away from the sample preparation station (12). A transport assembly (50) is configured to move at least one sample within the housing (16) and between the sample preparation station (12) and the sample analysis station (14).

摘要翻译： 样品制备和分析系统（10）。 系统（10）包括具有样品制备站（12）和位于壳体（16）内的样品分析站（14）的壳体（16）。 样品分析站（14）与样品制备站（12）间隔开。 运输组件（50）被配置为移动壳体（16）内和样品制备站（12）与样品分析站（14）之间的至少一个样品。

公开(公告)号：US20170168029A1

公开(公告)日：2017-06-15

申请号：US15445826

申请日：2017-02-28

申请人： Joseph L. Herman ,  Robert Dewitte ,  Dayana Argoti

发明人： Joseph L. Herman ,  Robert Dewitte ,  Dayana Argoti

IPC分类号： G01N30/34 ,  G01N33/82 ,  G01N33/94 ,  G01N30/72 ,  G01N30/88

CPC分类号： G01N30/7233 ,  G01N30/32 ,  G01N30/34 ,  G01N30/88 ,  G01N33/6848 ,  G01N33/82 ,  G01N33/9493 ,  G01N2030/8813 ,  G01N2430/00

摘要： Systems, apparatuses, kits, and methods for purification and analysis of analytes having a broad range of hydrophobicities by liquid chromatography-mass spectrometry (LC-MS). Using one set of liquid chromatography columns, one set of mobile phase buffers, and, optionally, a single ionization method (e.g., electrospray ionization), a wide range of analytes can be purified and analyzed on a liquid chromatography-mass spectrometry (LC-MS) system. LC-MS purification and analysis of analytes having a broad range of partition coefficients is accomplished by selecting LC run parameters and MS system parameters that are particular to different classes of analytes without having to make column or buffer changes or any other hardware configuration changes to the LC-MS system. The methods, systems, and kits described herein provide for substantially increased speed/throughput and ease of use for a wide range analytes with essentially no compromise in specificity for individual analytes relative to previously described methods.

公开(公告)号：US20110151719A1

公开(公告)日：2011-06-23

申请号：US12644289

申请日：2009-12-22

申请人： THOMAS ROBERT DeWITTE

发明人： THOMAS ROBERT DeWITTE

IPC分类号： H01R13/68

CPC分类号： H01R13/688 ,  B60L11/1818 ,  H01R2201/12 ,  Y02T10/7005 ,  Y02T10/7072 ,  Y02T90/14

摘要： A connector assembly includes a housing, an input conductor, and an output conductor. The housing is configured to mate with a mating connector. The input conductor is held in the housing and extends between a receiving end and a coupling end. The receiving end is configured to receive electric current from a power source. The coupling end is configured to join with a fuse inside the housing. The output conductor is held in the housing and extends between a transmission end and a joining end. The joining end is configured to couple with the fuse inside the housing. The transmission end is configured to convey the electric current to a first electric load. The input conductor and the output conductor are electrically coupled with one another via the fuse to provide a fused conductive pathway within the connector assembly over which the electric current passes from the power source to the first electric load.

摘要翻译： 连接器组件包括壳体，输入导体和输出导体。 壳体配置成与配合连接器配合。 输入导体被保持在壳体中并且在接收端和耦合端之间延伸。 接收端被配置为从电源接收电流。 联接端被配置成与壳体内的保险丝连接。 输出导体保持在壳体中并在传输端和接合端之间延伸。 接合端配置成与壳体内的保险丝耦合。 传输端构造成将电流传送到第一电负载。 输入导体和输出导体经由熔丝彼此电耦合，以在电连接器组件内提供熔融的导电通路，电流从该电源通过第一电负载。

公开(公告)号：US10088460B2

公开(公告)日：2018-10-02

申请号：US13882393

申请日：2011-10-28

申请人： Robert DeWitte ,  Juhani Siidorov ,  Vesa Nuotio ,  Raimo Salminen ,  Jarmo Vehkomaki ,  Jukka Saukkonen ,  Bill Ostman ,  Joseph M. Senteno ,  John Edward Brann, III ,  Joseph L. Herman ,  Jeffrey A. Zonderman ,  Terry N. Olney

发明人： Robert DeWitte ,  Juhani Siidorov ,  Vesa Nuotio ,  Raimo Salminen ,  Jarmo Vehkomaki ,  Jukka Saukkonen ,  Bill Ostman ,  Joseph M. Senteno ,  John Edward Brann, III ,  Joseph L. Herman ,  Jeffrey A. Zonderman ,  Terry N. Olney

IPC分类号： G01N30/72 ,  G01N30/06 ,  G01N30/86 ,  G01N35/00 ,  G01N35/02 ,  G01N35/08 ,  G01N27/62 ,  H01J49/04 ,  H01J49/00 ,  H01J49/10 ,  G01N30/88 ,  G01N30/16 ,  G01N30/02 ,  G01N30/62

摘要： A sample preparation and analysis system. The system 10 includes a sample preparation system 12 and a sample analysis system 14. The sample preparation system 12 prepares samples in accordance with an assay that is selected from a database containing a plurality of unique assays. The sample analysis system 14 includes an analyzer 110 that is dynamically reconfigurable based on the selected assay so as to analyze the prepared sample in accordance with that selected assay. A data communication link 27 communicates data from the sample preparation system 12 to the sample analysis system 14 to reconfigure the analyzer 110 in accordance with the selected assay.

公开(公告)号：US08221165B2

公开(公告)日：2012-07-17

申请号：US12644289

申请日：2009-12-22

申请人： Thomas Robert DeWitte

发明人： Thomas Robert DeWitte

IPC分类号： H01R13/68

CPC分类号： H01R13/688 ,  B60L11/1818 ,  H01R2201/12 ,  Y02T10/7005 ,  Y02T10/7072 ,  Y02T90/14

摘要： A connector assembly includes a housing, an input conductor, and an output conductor. The housing is configured to mate with a mating connector. The input conductor is held in the housing and extends between a receiving end and a coupling end. The receiving end is configured to receive electric current from a power source. The coupling end is configured to join with a fuse inside the housing. The output conductor is held in the housing and extends between a transmission end and a joining end. The joining end is configured to couple with the fuse inside the housing. The transmission end is configured to convey the electric current to a first electric load. The input conductor and the output conductor are electrically coupled with one another via the fuse to provide a fused conductive pathway within the connector assembly over which the electric current passes from the power source to the first electric load.

摘要翻译： 连接器组件包括壳体，输入导体和输出导体。 壳体配置成与配合连接器配合。 输入导体被保持在壳体中并且在接收端和耦合端之间延伸。 接收端被配置为从电源接收电流。 联接端被配置成与壳体内的保险丝连接。 输出导体保持在壳体中并在传输端和接合端之间延伸。 接合端配置成与壳体内的保险丝耦合。 传输端构造成将电流传送到第一电负载。 输入导体和输出导体经由熔丝彼此电耦合，以在电连接器组件内提供熔融的导电通路，电流从该电源通过第一电负载。

公开(公告)号：US20140047906A1

公开(公告)日：2014-02-20

申请号：US13881848

申请日：2011-10-28

申请人： Joseph L. Herman ,  Robert Dewitte ,  Dayana Argoti

发明人： Joseph L. Herman ,  Robert Dewitte ,  Dayana Argoti

IPC分类号： G01N30/72

CPC分类号： G01N30/7233 ,  G01N30/32 ,  G01N30/34 ,  G01N30/88 ,  G01N33/6848 ,  G01N33/82 ,  G01N33/9493 ,  G01N2030/8813 ,  G01N2430/00

摘要： Systems, apparatuses, kits, and methods for purification and analysis of analytes having a broad range of hydrophobicities by liquid chromatography-mass spectrometry (LC-MS). Using one set of liquid chromatography columns, one set of mobile phase buffers, and, optionally, a single ionization method (e.g., electrospray ionization), a wide range of analytes can be purified and analyzed on a liquid chromatography-mass spectrometry (LC-MS) system. LC-MS purification and analysis of analytes having a broad range of partition coefficients is accomplished by selecting LC run parameters and MS system parameters that are particular to different classes of analytes without having to make column or buffer changes or any other hardware configuration changes to the LC-MS system. The methods, systems, and kits described herein provide for substantially increased speed/throughput and ease of use for a wide range analytes with essentially no compromise in specificity for individual analytes relative to previously described methods.

摘要翻译： 通过液相色谱 - 质谱（LC-MS）纯化和分析具有广泛疏水性的分析物的系统，设备，试剂盒和方法。 使用一套液相色谱柱，一组流动相缓冲液，以及任选的单一离子化方法（如电喷雾离子化），可以在液相色谱 - 质谱（LC- MS）系统。 通过选择不同类别分析物特有的LC运行参数和MS系统参数，而不必对列或缓冲区进行更改或对其进行任何其他硬件配置更改，从而实现具有宽范围分配系数的分析物的LC-MS纯化和分析。 LC-MS系统。 本文描述的方法，系统和试剂盒提供了对于宽范围分析物的显着提高的速度/通量和易用性，其相对于先前描述的方法基本上不损害针对各个分析物的特异性。