公开(公告)号：US09278108B2

公开(公告)日：2016-03-08

申请号：US13383826

申请日：2010-07-16

申请人： Hiromi Takenaka ,  Jou Akitomi ,  Shintarou Katou ,  Shotaro Tsuji ,  Takashi Ohtsu ,  Iwao Waga

发明人： Hiromi Takenaka ,  Jou Akitomi ,  Shintarou Katou ,  Shotaro Tsuji ,  Takashi Ohtsu ,  Iwao Waga

IPC分类号： C12N15/115 ,  A61K31/7088 ,  A61K31/7105 ,  C07K14/47 ,  G01N33/68

CPC分类号： A61K31/7088 ,  A61K31/7105 ,  C07K14/4703 ,  C12N15/115 ,  C12N2310/16 ,  G01N33/6863

摘要： A nucleic acid molecule that can bind to HMGB1 protein and applications thereof are provided. A nucleic acid molecule having a dissociation constant for HMGB1 protein of 5×10−7 or less can be used as the nucleic acid molecule that can bind to HMGB1 protein. The HMGB1 binding nucleic acid molecule can bind to HMGB1 protein that is known to be a cause of diseases such as cancer and inflammation, and it is therefore possible to obtain an effect to prevent and an effect to treat such diseases by allowing the HMGB1 binding nucleic acid molecule to bind to HMGB1 protein in a living body.

摘要翻译： 提供可结合HMGB1蛋白的核酸分子及其应用。 具有5×10 -7以下的HMGB1蛋白的解离常数的核酸分子可以用作能结合HMGB1蛋白的核酸分子。 HMGB1结合核酸分子可以结合已知是诸如癌症和炎症等疾病的原因的HMGB1蛋白，因此可以通过使HMGB1结合核酸分子获得预防和治疗这些疾病的效果， 酸分子结合活体中的HMGB1蛋白。

公开(公告)号：US08822667B2

公开(公告)日：2014-09-02

申请号：US13812190

申请日：2011-07-26

申请人： Naomi Hirabayashi ,  Shotaro Tsuji ,  Jou Akitomi ,  Shintarou Katou ,  Iwao Waga ,  Takashi Ohtsu

发明人： Naomi Hirabayashi ,  Shotaro Tsuji ,  Jou Akitomi ,  Shintarou Katou ,  Iwao Waga ,  Takashi Ohtsu

IPC分类号： C07H21/04 ,  C07H21/02 ,  A61K48/00

CPC分类号： C07H21/02 ,  A61K31/52 ,  A61K31/7105 ,  C12N15/115 ,  C12N2310/16 ,  C12Q1/6883 ,  C12Q2525/301 ,  C12Q2527/125 ,  A61K2300/00

摘要： The present invention provides a nucleic acid molecule capable of binding to c-Met as a substance that can be used for clarification of the pathogenic mechanism of diseases caused by c-Met, diagnosis and treatment of the diseases, and the like, and also the use thereof. The c-Met binding nucleic acid molecule of the present invention is any one of the following nucleic acid molecules (A1), (A2), (B1), and (B2). (A1) a nucleic acid molecule including the base sequence of any one of SEQ ID NOs: 1 to 38 (A2) a nucleic acid molecule that is capable of binding to c-Met and includes a base sequence obtained by substitution, deletion, addition, and/or insertion of one or more bases in the base sequence of any one of SEQ ID NOs: 1 to 38 (B1) a nucleic acid molecule including the base sequence of any one of SEQ ID NOs: 39 to 76 (B2) a nucleic acid molecule that is capable of binding to c-Met and includes a base sequence obtained by substitution, deletion, addition, and/or insertion of one or more bases in the base sequence of any one of SEQ ID NOs: 39 to 76.

摘要翻译： 本发明提供能够结合c-Met的核酸分子，作为能够用于澄清由c-Met，疾病的诊断和治疗等引起的疾病的致病机制的物质，以及 使用它。 本发明的c-Met结合核酸分子是以下核酸分子（A1），（A2），（B1）和（B2）中的任一种。 （A1）包含SEQ ID NO：1至38（A2）中任一个的碱基序列的核酸分子，其能够结合c-Met并包含通过取代，缺失，加成获得的碱基序列 ，（SEQ ID NO：39）至（38）中的任何一个的碱基序列的核酸分子，和/或在SEQ ID NO：1至38（B1）中任一个的碱基序列中插入一个或多个碱基， 能够结合c-Met的核酸分子，并且包含通过SEQ ID NO：39至76中任一个的碱基序列中的一个或多个碱基的取代，缺失，添加和/或插入获得的碱基序列 。

公开(公告)号：US09382544B2

公开(公告)日：2016-07-05

申请号：US13320462

申请日：2010-05-14

申请人： Shotaro Tsuji ,  Jou Akitomi ,  Shintarou Katou ,  Iwao Waga ,  Takashi Ohtsu

发明人： Shotaro Tsuji ,  Jou Akitomi ,  Shintarou Katou ,  Iwao Waga ,  Takashi Ohtsu

IPC分类号： C12N15/115 ,  C07H21/02 ,  C12N15/113 ,  C12N15/10

CPC分类号： C12N15/115 ,  C07H21/02 ,  C12N15/1048 ,  C12N15/113 ,  C12N2310/113 ,  C12N2310/16 ,  C12N2320/50

摘要： An aptamer capable of binding to a histidine peptide is provided. A nucleic acid used as the aptamer capable of binding to a histidine peptide may be a nucleic acid containing the base sequence of SEQ ID NO: 17, SEQ ID NO: 18, or containing a base sequence obtained by substitution, deletion, addition, or insertion of one or more bases in SEQ ID NO: 17 or 18.

摘要翻译： 提供了能够结合组氨酸肽的适体。 用作能够结合组氨酸肽的适体的核酸可以是含有SEQ ID NO：17，SEQ ID NO：18的碱基序列或含有通过取代，缺失，添加或 在SEQ ID NO：17或18中插入一个或多个碱基。

公开(公告)号：US20120129720A1

公开(公告)日：2012-05-24

申请号：US13320462

申请日：2010-05-14

申请人： Shotaro Tsuji ,  Jou Akitomi ,  Shintarou Katou ,  Iwao Waga ,  Takashi Ohtsu

发明人： Shotaro Tsuji ,  Jou Akitomi ,  Shintarou Katou ,  Iwao Waga ,  Takashi Ohtsu

IPC分类号： C40B30/04 ,  C12N15/63 ,  C12N15/115 ,  C07H21/02

CPC分类号： C12N15/115 ,  C07H21/02 ,  C12N15/1048 ,  C12N15/113 ,  C12N2310/113 ,  C12N2310/16 ,  C12N2320/50

摘要： An aptamer capable of binding to a histidine peptide is provided. A nucleic acid used as the aptamer capable of binding to a histidine peptide is any of the following nucleic acids (a) to (d): (a) a nucleic acid having a base sequence represented by SEQ ID NO: 17: GGUNnAYUmGGH (SEQ ID NO: 17), where in the nucleic acid (a), N represents A, G, C, U, or T, n of Nn represents the number of Ns and is an integer from 1 to 3, Y represents U, T, or C, m of Um represents the number of Us and is an integer from 1 to 3, and H represents U, T, C, or A; (b) a nucleic acid having a base sequence obtained by substitution, deletion, addition, or insertion of one or more bases in a base sequence of the nucleic acid (a) and being capable of binding to the histidine peptide; (c) a nucleic acid having a base sequence represented by SEQ ID NO: 18: GGCGCCUUCGUGGAAUGUC (SEQ ID NO: 18); and (d) a nucleic acid having a base sequence obtained by substitution, deletion, addition, or insertion of one or more bases in a base sequence of the nucleic acid (c) and being capable of binding to the histidine peptide.

摘要翻译： 提供了能够结合组氨酸肽的适体。 用作能够结合组氨酸肽的适体的核酸是以下任一核酸（a）至（d）：（a）具有由SEQ ID NO：17表示的碱基序列的核酸：GGUNnAYUmGGH（SEQ ID NO： ID号：17），其中在核酸（a）中，N表示A，G，C，U或T，N n表示N的数，为1〜3的整数，Y表示U，T 或C m表示Us的数，是1〜3的整数，H表示U，T，C或A; （b）具有通过在核酸（a）的碱基序列中取代，缺失，添加或插入一个或多个碱基并且能够结合组氨酸肽而获得的碱基序列的核酸; （c）具有由SEQ ID NO：18表示的碱基序列的核酸：GGCGCCUUCGUGGAAUGUC（SEQ ID NO：18）; 和（d）具有通过在核酸（c）的碱基序列中取代，缺失，添加或插入一个或多个碱基并且能够结合组氨酸肽而获得的碱基序列的核酸。

公开(公告)号：US09454642B2

公开(公告)日：2016-09-27

申请号：US13807876

申请日：2011-07-02

申请人： Jou Akitomi ,  Shintarou Katou ,  Shotaro Tsuji ,  Iwao Waga

发明人： Jou Akitomi ,  Shintarou Katou ,  Shotaro Tsuji ,  Takashi Ohtsu ,  Iwao Waga

IPC分类号： G01N33/50 ,  G06F19/16 ,  C12N15/115 ,  G06F19/18

CPC分类号： G06F19/16 ,  C12N15/115 ,  C12N2310/16 ,  G06F19/18

摘要： The present invention provides a prediction device, a prediction method, a program, and a recording medium, with which whether or not desired aptamer sequences are enriched can be predicted easily. The prediction device of the present invention 10 includes an input unit 11, a calculation unit 12, and a prediction unit 13. The input unit 11 is a unit through which sequence information on a target aptamer sequence group including selected aptamers in a target pool and a reference aptamer sequence group including reference aptamer sequences are inputted. The calculation unit 12 calculates the free energy of the target aptamer sequence group and the free energy of the reference aptamer sequence group. The prediction unit 13 compares the free energy of these sequence groups, and predicts that the target pool is an enriched pool when the free energy of the target aptamer sequence group is lower than the free energy of the reference aptamer sequence group. The reference aptamer sequence group is a candidate aptamer sequence group including a plurality of candidate aptamer sequences or a virtual aptamer sequence group that is derived from the target aptamer sequence group and includes virtual aptamer sequences having the same base composition as the target aptamer sequence group.

摘要翻译： 本发明提供一种预测装置，预测方法，程序和记录介质，可以容易地预测其中是否需要富集需要的适体序列。 本发明的预测装置10包括输入单元11，计算单元12和预测单元13.输入单元11是通过目标池中包括所选择的适配体的目标适体序列组的序列信息和 输入包括参考适体序列的参照适体序列组。 计算单元12计算目标适体序列组的自由能和参考适体序列组的自由能。 预测单元13比较这些序列组的自由能，并且当靶适体序列组的自由能低于参考适体序列组的自由能时，预测目标池是富集池。 参照适体序列组是包含从目标适体序列组得到的多个候选适体序列或虚拟适体序列组的候选适体序列组，并且具有与靶适体序列组相同的基本组成的虚拟适配子序列。

公开(公告)号：US20130102480A1

公开(公告)日：2013-04-25

申请号：US13807876

申请日：2011-07-02

申请人： Jou Akitomi ,  Shintarou Katou ,  Shotaro Tsuji ,  Takashi Ohtsu ,  Iwao Waga

发明人： Jou Akitomi ,  Shintarou Katou ,  Shotaro Tsuji ,  Takashi Ohtsu ,  Iwao Waga

IPC分类号： G06F19/16

CPC分类号： G06F19/16 ,  C12N15/115 ,  C12N2310/16 ,  G06F19/18

摘要： The present invention provides a prediction device, a prediction method, a program, and a recording medium, with which whether or not desired aptamer sequences are enriched can be predicted easily. The prediction device of the present invention 10 includes an input unit 11, a calculation unit 12, and a prediction unit 13. The input unit 11 is a unit through which sequence information on a target aptamer sequence group including selected aptamers in a target pool and a reference aptamer sequence group including reference aptamer sequences are inputted. The calculation unit 12 calculates the free energy of the target aptamer sequence group and the free energy of the reference aptamer sequence group. The prediction unit 13 compares the free energy of these sequence groups, and predicts that the target pool is an enriched pool when the free energy of the target aptamer sequence group is lower than the free energy of the reference aptamer sequence group. The reference aptamer sequence group is a candidate aptamer sequence group including a plurality of candidate aptamer sequences or a virtual aptamer sequence group that is derived from the target aptamer sequence group and includes virtual aptamer sequences having the same base composition as the target aptamer sequence group.

摘要翻译： 本发明提供一种预测装置，预测方法，程序和记录介质，可以容易地预测其中是否需要富集需要的适体序列。 本发明的预测装置10包括输入单元11，计算单元12和预测单元13.输入单元11是通过目标池中包括所选择的适配体的目标适体序列组的序列信息和 输入包括参考适体序列的参照适体序列组。 计算单元12计算目标适体序列组的自由能和参考适体序列组的自由能。 预测单元13比较这些序列组的自由能，并且当靶适体序列组的自由能低于参考适体序列组的自由能时，预测目标池是富集池。 参照适体序列组是包含从目标适体序列组得到的多个候选适体序列或虚拟适体序列组的候选适体序列组，并且具有与靶适体序列组相同的基本组成的虚拟适配子序列。

公开(公告)号：US20130123350A1

公开(公告)日：2013-05-16

申请号：US13812190

申请日：2011-07-26

申请人： Naomi Hirabayashi ,  Shotaro Tsuji ,  Jou Akitomi ,  Shintarou Katou ,  Iwao Waga ,  Takashi Ohtsu

发明人： Naomi Hirabayashi ,  Shotaro Tsuji ,  Jou Akitomi ,  Shintarou Katou ,  Iwao Waga ,  Takashi Ohtsu

IPC分类号： C07H21/02

CPC分类号： C07H21/02 ,  A61K31/52 ,  A61K31/7105 ,  C12N15/115 ,  C12N2310/16 ,  C12Q1/6883 ,  C12Q2525/301 ,  C12Q2527/125 ,  A61K2300/00

摘要： The present invention provides a nucleic acid molecule capable of binding to c-Met as a substance that can be used for clarification of the pathogenic mechanism of diseases caused by c-Met, diagnosis and treatment of the diseases, and the like, and also the use thereof. The c-Met binding nucleic acid molecule of the present invention is any one of the following nucleic acid molecules (A1), (A2), (B1), and (B2).(A1) a nucleic acid molecule including the base sequence of any one of SEQ ID NOs: 1 to 38 (A2) a nucleic acid molecule that is capable of binding to c-Met and includes a base sequence obtained by substitution, deletion, addition, and/or insertion of one or more bases in the base sequence of any one of SEQ ID NOs: 1 to 38 (B1) a nucleic acid molecule including the base sequence of any one of SEQ ID NOs: 39 to 76 (B2) a nucleic acid molecule that is capable of binding to c-Met and includes a base sequence obtained by substitution, deletion, addition, and/or insertion of one or more bases in the base sequence of any one of SEQ ID NOs: 39 to 76

摘要翻译： 本发明提供能够结合c-Met的核酸分子，作为能够用于澄清由c-Met，疾病的诊断和治疗等引起的疾病的致病机制的物质，以及 使用它。 本发明的c-Met结合核酸分子是以下核酸分子（A1），（A2），（B1）和（B2）中的任一种。 （A1）包含SEQ ID NO：1至38（A2）中任一个的碱基序列的核酸分子，其能够结合c-Met并包含通过取代，缺失，加成获得的碱基序列 ，（SEQ ID NO：39）至（38）中的任何一个的碱基序列的核酸分子，和/或在SEQ ID NO：1至38（B1）中任一个的碱基序列中插入一个或多个碱基， 能够结合c-Met的核酸分子，并且包含通过SEQ ID NO：39至76中任一个的碱基序列中的一个或多个碱基的取代，缺失，添加和/或插入获得的碱基序列

公开(公告)号：US09557339B2

公开(公告)日：2017-01-31

申请号：US14336723

申请日：2014-07-21

申请人： Hiromi Takenaka ,  Yoshihito Yoshida ,  Katsunori Horii ,  Makio Furuichi ,  Hirotaka Yagi ,  Jou Akitomi ,  Mineko Yamaguchi ,  Shintarou Katou ,  Kensaku Nishikata ,  Iwao Waga

发明人： Hiromi Takenaka ,  Yoshihito Yoshida ,  Katsunori Horii ,  Makio Furuichi ,  Hirotaka Yagi ,  Jou Akitomi ,  Mineko Yamaguchi ,  Shintarou Katou ,  Kensaku Nishikata ,  Iwao Waga

IPC分类号： C12N15/115 ,  G01N33/68 ,  G01N33/53

CPC分类号： G01N33/6854 ,  C12N15/115 ,  C12N2310/16 ,  G01N33/5308

摘要： The present invention is to provide a nucleic acid molecule having a binding affinity to a rodent-derived IgG antibody, which can be prepared easier than an antibody and has a binding affinity equivalent or superior to that of an antibody, a binder using the nucleic acid molecule, a detection reagent, and a detection kit. The nucleic acid molecule of the present invention has a binding affinity to a rodent-derived IgG antibody and has a dissociation constant of 1 μM or less. The binder for a rodent-derived IgG antibody of the present invention includes the nucleic acid molecule of the present invention. The detection reagent for detecting a rodent-derived IgG antibody of the present invention includes the binder for a rodent-derived IgG antibody of the present invention. The detection kit for detecting a rodent-derived IgG antibody of the present invention includes the detection reagent for detecting a rodent-derived IgG antibody of the present invention.

摘要翻译： 本发明提供一种对啮齿动物IgG抗体具有结合亲和力的核酸分子，其可以比抗体更容易制备并具有与抗体相当或优于其抗体的结合亲和力，使用该核酸的粘合剂 分子，检测试剂和检测试剂盒。 本发明的核酸分子与啮齿动物IgG抗体具有结合亲和力，解离常数为1μM以下。 本发明的啮齿动物IgG抗体的粘合剂包括本发明的核酸分子。 用于检测本发明的啮齿动物IgG抗体的检测试剂包括本发明的啮齿动物IgG抗体的粘合剂。 用于检测本发明的啮齿动物IgG抗体的检测试剂盒包括用于检测本发明的啮齿动物的IgG抗体的检测试剂。

公开(公告)号：US20140128589A1

公开(公告)日：2014-05-08

申请号：US14129392

申请日：2012-07-02

申请人： Naoto Kaneko ,  Katsunori Horii ,  Jou Akitomi ,  Shintarou Katou ,  Iwao Waga

发明人： Naoto Kaneko ,  Katsunori Horii ,  Jou Akitomi ,  Shintarou Katou ,  Iwao Waga

IPC分类号： C12N15/113 ,  G01N27/26 ,  G01N33/58

CPC分类号： C12N15/113 ,  C12N15/1034 ,  C12N15/111 ,  C12N15/115 ,  C12N2310/127 ,  C12N2310/16 ,  C12N2310/351 ,  C12N2320/10 ,  C12N2330/31 ,  C12Q1/26 ,  C12Q1/6825 ,  G01N27/26 ,  G01N33/581 ,  C12Q2304/80 ,  C12Q2525/205 ,  C12Q2563/113

摘要： The present invention provides a novel technique by which the redox activity of a nucleic acid molecule can be evaluated. An evaluation method of the present invention includes: a detection step of electrochemically detecting a redox reaction to a substrate, the redox reaction being catalyzed by a nucleic acid molecule to be evaluated, using a device that electrochemically detects a redox reaction; and an evaluation step of evaluating redox activity of the nucleic acid molecule from a result of the detection of the redox reaction. As the device, a device in which a base provided with a detection portion is included, the detection portion.includes an electrode system, and the nucleic acid molecule to be evaluated is arranged on the base is used. In the present invention, it is preferred that a plurality of kinds of nucleic acid molecule to be evaluated is arranged on the base, and the plurality of kinds of nucleic acid molecules to be evaluated is evaluated by a single device.

摘要翻译： 本发明提供可以评价核酸分子的氧化还原活性的新技术。 本发明的评价方法包括：使用电化学检测氧化还原反应的装置电化学检测对基底的氧化还原反应的检测步骤，所述氧化还原反应由待评价的核酸分子催化; 以及从检测氧化还原反应的结果来评价核酸分子的氧化还原活性的评价步骤。 作为该装置，包括具有检测部的基部的检测部。 包括电极系统，并且使用要评估的核酸分子设置在基底上。 在本发明中，优选在基材上排列要评价的多种核酸分子，通过单个装置评价要评价的多种核酸分子。

公开(公告)号：US20130273530A1

公开(公告)日：2013-10-17

申请号：US13996389

申请日：2011-12-22

申请人： Katsunori Horii ,  Shintarou Katou ,  Jou Akitomi ,  Iwao Waga

发明人： Katsunori Horii ,  Shintarou Katou ,  Jou Akitomi ,  Iwao Waga

IPC分类号： C12Q1/68

CPC分类号： C12Q1/68 ,  C12N15/111 ,  C12N2310/16 ,  C12N2320/10 ,  C12Q1/6834 ,  G01N33/54306 ,  C12Q2525/205 ,  C12Q2563/131

摘要： The present invention provides a technique capable of simply analyzing a target to be analyzed. An analytical device of the present invention includes a basal plate; a nucleic acid element; and a detection section of detecting a signal. The nucleic acid element and the detection section are arranged on the basal plate. The nucleic acid element includes a first nucleic acid molecule and a second nucleic acid molecule. The first nucleic acid molecule is a nucleic acid molecule capable of binding to a target. The second nucleic acid molecule is a nucleic acid molecule capable of binding to streptavidin. When the target does not bind to the first nucleic acid molecule, a binding capacity of the second nucleic acid molecule to the streptavidin is inactivated. When the target binds to the first nucleic acid molecule, a binding capacity of the second nucleic acid molecule to the streptavidin is activated. The detection section detects binding between the second nucleic acid molecule and the streptavidin. The target is bound to the first nucleic acid molecule, so that the streptavidin is bound to the second nucleic acid molecule. Thus, the target can be analyzed through detecting the binding between the second nucleic acid molecule and the streptavidin using the detection device.

摘要翻译： 本发明提供能够简单地分析要分析的靶的技术。 本发明的分析装置包括基板; 核酸元件; 以及检测信号的检测部。 核酸元件和检测部设置在基板上。 核酸元件包括第一核酸分子和第二核酸分子。 第一核酸分子是能够结合靶的核酸分子。 第二核酸分子是能够结合链霉亲和素的核酸分子。 当靶不与第一核酸分子结合时，第二核酸分子与链霉抗生物素蛋白的结合能力失活。 当靶标与第一核酸分子结合时，第二核酸分子与链亲和素的结合能力被激活。 检测部分检测第二核酸分子和链霉抗生物素蛋白之间的结合。 靶标与第一核酸分子结合，使得链亲和素与第二核酸分子结合。 因此，可以使用检测装置通过检测第二核酸分子和链霉亲和素之间的结合来分析靶。