摘要:
A nucleic acid molecule that can bind to HMGB1 protein and applications thereof are provided. A nucleic acid molecule having a dissociation constant for HMGB1 protein of 5×10−7 or less can be used as the nucleic acid molecule that can bind to HMGB1 protein. The HMGB1 binding nucleic acid molecule can bind to HMGB1 protein that is known to be a cause of diseases such as cancer and inflammation, and it is therefore possible to obtain an effect to prevent and an effect to treat such diseases by allowing the HMGB1 binding nucleic acid molecule to bind to HMGB1 protein in a living body.
摘要:
The present invention provides a nucleic acid molecule capable of binding to c-Met as a substance that can be used for clarification of the pathogenic mechanism of diseases caused by c-Met, diagnosis and treatment of the diseases, and the like, and also the use thereof. The c-Met binding nucleic acid molecule of the present invention is any one of the following nucleic acid molecules (A1), (A2), (B1), and (B2). (A1) a nucleic acid molecule including the base sequence of any one of SEQ ID NOs: 1 to 38 (A2) a nucleic acid molecule that is capable of binding to c-Met and includes a base sequence obtained by substitution, deletion, addition, and/or insertion of one or more bases in the base sequence of any one of SEQ ID NOs: 1 to 38 (B1) a nucleic acid molecule including the base sequence of any one of SEQ ID NOs: 39 to 76 (B2) a nucleic acid molecule that is capable of binding to c-Met and includes a base sequence obtained by substitution, deletion, addition, and/or insertion of one or more bases in the base sequence of any one of SEQ ID NOs: 39 to 76.
摘要翻译:本发明提供能够结合c-Met的核酸分子,作为能够用于澄清由c-Met,疾病的诊断和治疗等引起的疾病的致病机制的物质,以及 使用它。 本发明的c-Met结合核酸分子是以下核酸分子(A1),(A2),(B1)和(B2)中的任一种。 (A1)包含SEQ ID NO:1至38(A2)中任一个的碱基序列的核酸分子,其能够结合c-Met并包含通过取代,缺失,加成获得的碱基序列 ,(SEQ ID NO:39)至(38)中的任何一个的碱基序列的核酸分子,和/或在SEQ ID NO:1至38(B1)中任一个的碱基序列中插入一个或多个碱基, 能够结合c-Met的核酸分子,并且包含通过SEQ ID NO:39至76中任一个的碱基序列中的一个或多个碱基的取代,缺失,添加和/或插入获得的碱基序列 。
摘要:
An aptamer capable of binding to a histidine peptide is provided. A nucleic acid used as the aptamer capable of binding to a histidine peptide may be a nucleic acid containing the base sequence of SEQ ID NO: 17, SEQ ID NO: 18, or containing a base sequence obtained by substitution, deletion, addition, or insertion of one or more bases in SEQ ID NO: 17 or 18.
摘要翻译:提供了能够结合组氨酸肽的适体。 用作能够结合组氨酸肽的适体的核酸可以是含有SEQ ID NO:17,SEQ ID NO:18的碱基序列或含有通过取代,缺失,添加或 在SEQ ID NO:17或18中插入一个或多个碱基。
摘要:
An aptamer capable of binding to a histidine peptide is provided. A nucleic acid used as the aptamer capable of binding to a histidine peptide is any of the following nucleic acids (a) to (d): (a) a nucleic acid having a base sequence represented by SEQ ID NO: 17: GGUNnAYUmGGH (SEQ ID NO: 17), where in the nucleic acid (a), N represents A, G, C, U, or T, n of Nn represents the number of Ns and is an integer from 1 to 3, Y represents U, T, or C, m of Um represents the number of Us and is an integer from 1 to 3, and H represents U, T, C, or A; (b) a nucleic acid having a base sequence obtained by substitution, deletion, addition, or insertion of one or more bases in a base sequence of the nucleic acid (a) and being capable of binding to the histidine peptide; (c) a nucleic acid having a base sequence represented by SEQ ID NO: 18: GGCGCCUUCGUGGAAUGUC (SEQ ID NO: 18); and (d) a nucleic acid having a base sequence obtained by substitution, deletion, addition, or insertion of one or more bases in a base sequence of the nucleic acid (c) and being capable of binding to the histidine peptide.
摘要翻译:提供了能够结合组氨酸肽的适体。 用作能够结合组氨酸肽的适体的核酸是以下任一核酸(a)至(d):(a)具有由SEQ ID NO:17表示的碱基序列的核酸:GGUNnAYUmGGH(SEQ ID NO: ID号:17),其中在核酸(a)中,N表示A,G,C,U或T,N n表示N的数,为1〜3的整数,Y表示U,T 或C m表示Us的数,是1〜3的整数,H表示U,T,C或A; (b)具有通过在核酸(a)的碱基序列中取代,缺失,添加或插入一个或多个碱基并且能够结合组氨酸肽而获得的碱基序列的核酸; (c)具有由SEQ ID NO:18表示的碱基序列的核酸:GGCGCCUUCGUGGAAUGUC(SEQ ID NO:18); 和(d)具有通过在核酸(c)的碱基序列中取代,缺失,添加或插入一个或多个碱基并且能够结合组氨酸肽而获得的碱基序列的核酸。
摘要:
The present invention provides a prediction device, a prediction method, a program, and a recording medium, with which whether or not desired aptamer sequences are enriched can be predicted easily. The prediction device of the present invention 10 includes an input unit 11, a calculation unit 12, and a prediction unit 13. The input unit 11 is a unit through which sequence information on a target aptamer sequence group including selected aptamers in a target pool and a reference aptamer sequence group including reference aptamer sequences are inputted. The calculation unit 12 calculates the free energy of the target aptamer sequence group and the free energy of the reference aptamer sequence group. The prediction unit 13 compares the free energy of these sequence groups, and predicts that the target pool is an enriched pool when the free energy of the target aptamer sequence group is lower than the free energy of the reference aptamer sequence group. The reference aptamer sequence group is a candidate aptamer sequence group including a plurality of candidate aptamer sequences or a virtual aptamer sequence group that is derived from the target aptamer sequence group and includes virtual aptamer sequences having the same base composition as the target aptamer sequence group.
摘要:
The present invention provides a nucleic acid molecule capable of binding to c-Met as a substance that can be used for clarification of the pathogenic mechanism of diseases caused by c-Met, diagnosis and treatment of the diseases, and the like, and also the use thereof. The c-Met binding nucleic acid molecule of the present invention is any one of the following nucleic acid molecules (A1), (A2), (B1), and (B2).(A1) a nucleic acid molecule including the base sequence of any one of SEQ ID NOs: 1 to 38 (A2) a nucleic acid molecule that is capable of binding to c-Met and includes a base sequence obtained by substitution, deletion, addition, and/or insertion of one or more bases in the base sequence of any one of SEQ ID NOs: 1 to 38 (B1) a nucleic acid molecule including the base sequence of any one of SEQ ID NOs: 39 to 76 (B2) a nucleic acid molecule that is capable of binding to c-Met and includes a base sequence obtained by substitution, deletion, addition, and/or insertion of one or more bases in the base sequence of any one of SEQ ID NOs: 39 to 76
摘要翻译:本发明提供能够结合c-Met的核酸分子,作为能够用于澄清由c-Met,疾病的诊断和治疗等引起的疾病的致病机制的物质,以及 使用它。 本发明的c-Met结合核酸分子是以下核酸分子(A1),(A2),(B1)和(B2)中的任一种。 (A1)包含SEQ ID NO:1至38(A2)中任一个的碱基序列的核酸分子,其能够结合c-Met并包含通过取代,缺失,加成获得的碱基序列 ,(SEQ ID NO:39)至(38)中的任何一个的碱基序列的核酸分子,和/或在SEQ ID NO:1至38(B1)中任一个的碱基序列中插入一个或多个碱基, 能够结合c-Met的核酸分子,并且包含通过SEQ ID NO:39至76中任一个的碱基序列中的一个或多个碱基的取代,缺失,添加和/或插入获得的碱基序列
摘要:
The present invention provides a prediction device, a prediction method, a program, and a recording medium, with which whether or not desired aptamer sequences are enriched can be predicted easily. The prediction device of the present invention 10 includes an input unit 11, a calculation unit 12, and a prediction unit 13. The input unit 11 is a unit through which sequence information on a target aptamer sequence group including selected aptamers in a target pool and a reference aptamer sequence group including reference aptamer sequences are inputted. The calculation unit 12 calculates the free energy of the target aptamer sequence group and the free energy of the reference aptamer sequence group. The prediction unit 13 compares the free energy of these sequence groups, and predicts that the target pool is an enriched pool when the free energy of the target aptamer sequence group is lower than the free energy of the reference aptamer sequence group. The reference aptamer sequence group is a candidate aptamer sequence group including a plurality of candidate aptamer sequences or a virtual aptamer sequence group that is derived from the target aptamer sequence group and includes virtual aptamer sequences having the same base composition as the target aptamer sequence group.
摘要:
A nucleic acid molecule that can bind to HMGB1 protein and applications thereof are provided. A nucleic acid molecule having a dissociation constant for HMGB1 protein of 5×10−7 or less can be used as the nucleic acid molecule that can bind to HMGB1 protein. The HMGB1 binding nucleic acid molecule can bind to HMGB1 protein that is known to be a cause of diseases such as cancer and inflammation, and it is therefore possible to obtain an effect to prevent and an effect to treat such diseases by allowing the HMGB1 binding nucleic acid molecule to bind to HMGB1 protein in a living body.
摘要:
The present invention is to provide a new detection method that is superior in detection sensitivity and allows a simple operation. A nucleic acid construct that includes a cloning region, an encoding nucleic acid of a peptide tag, an encoding nucleic acid of an aptamer that is bindable to the peptide tag is used. By inserting the encoding nucleic acid of arbitrary peptide into the cloning region of the nucleic acid construct, the nucleic acid construct is expressed in vivo. Thereby, a complex of a fusion transcript having the base sequence that includes the encoding nucleic acid of arbitrary peptide, the encoding nucleic acid of a peptide tag, and the encoding nucleic acid of the aptamer and a fusion translation that includes the arbitrary peptide and the peptide tag is formed. By bringing the complex into contact with a target and recovering the complex that is bound to the target, the peptide that is bindable to a target and its encoding nucleic acid can be identified from the transcript of the encoding nucleic acid of arbitrary peptide in the complex.
摘要:
The TV receiver as a display device has a drawing generation unit for generating display side drawing data of drawn picture according to a display format for on-screen display, and a reproducing apparatus has a drawing generation unit for generating reproduction side drawing data of drawn picture according to a display format for display on the TV receiver. The drawing generation unit receives the display format information of the drawing generation unit through a connection cable, and generates reproduction side drawing data of display format based on the received display side display format information. The reproducing apparatus supplies the contents data including the generated reproduction side drawing data to the TV receiver through the connection cable, and the TV receiver presents the drawn picture based on the supplied reproduction side drawing data.