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公开(公告)号:US08026068B2
公开(公告)日:2011-09-27
申请号:US10337190
申请日:2003-01-06
申请人: Judith Pinsl-Ober , Peter Wenzig , Ralf Schoenbrunner , Patrick O'Donnell , Erich Kyger , Khushbeer Malhotra , Kurt Weindel , Knut Bartl
发明人: Judith Pinsl-Ober , Peter Wenzig , Ralf Schoenbrunner , Patrick O'Donnell , Erich Kyger , Khushbeer Malhotra , Kurt Weindel , Knut Bartl
IPC分类号: C12P19/34
CPC分类号: C12N15/1006 , C12Q1/6806 , C12Q2547/101 , C12Q2531/113
摘要: This invention relates to a method for the isolation of a target nucleic acid using a material with an unmodified silica surface and subsequent amplification of the target nucleic acid in the presence of the material with an unmodified silica surface. The method is preferably carried out as an automated process preferably in a high throughput format. The method is preferably used in diagnostics.
摘要翻译: 本发明涉及使用具有未修饰的二氧化硅表面的材料分离靶核酸的方法,并且在具有未改性的二氧化硅表面的材料存在下随后扩增靶核酸。 该方法优选以高通量格式作为自动化方法进行。 该方法优选用于诊断。
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公开(公告)号:US20110172408A1
公开(公告)日:2011-07-14
申请号:US12684762
申请日:2010-01-08
申请人: Jenny A. Johnson , Erich Kyger
发明人: Jenny A. Johnson , Erich Kyger
IPC分类号: C07H1/06
CPC分类号: C12N15/1006 , C12N15/1013 , C12Q1/6834 , C12Q2527/125 , C12Q2563/143
摘要: The present invention is a method of separating nucleic acids using a solid phase capable of binding nucleic acids, such as magnetic glass particles, where binding of nucleic acids to the solid phase is enhanced by the presence of an ethylene-amine compound. The invention further includes a reaction mixture for isolating nucleic acids containing an ethylene-amine compound and kits for carrying out the method.
摘要翻译: 本发明是使用能够结合核酸的固相(例如磁性玻璃颗粒)分离核酸的方法,其中核酸与固相的结合通过乙烯 - 胺化合物的存在得到增强。 本发明还包括用于分离含有乙烯胺化合物的核酸的反应混合物和用于实施该方法的试剂盒。
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公开(公告)号:US08420801B2
公开(公告)日:2013-04-16
申请号:US12684762
申请日:2010-01-08
申请人: Jenny A. Johnson , Erich Kyger
发明人: Jenny A. Johnson , Erich Kyger
IPC分类号: C07H21/00
CPC分类号: C12N15/1006 , C12N15/1013 , C12Q1/6834 , C12Q2527/125 , C12Q2563/143
摘要: The present invention is a method of separating nucleic acids using a solid phase capable of binding nucleic acids, such as magnetic glass particles, where binding of nucleic acids to the solid phase is enhanced by the presence of an ethylene-amine compound. The invention further includes a reaction mixture for isolating nucleic acids containing an ethylene-amine compound and kits for carrying out the method.
摘要翻译: 本发明是使用能够结合核酸的固相(例如磁性玻璃颗粒)分离核酸的方法,其中核酸与固相的结合通过乙烯 - 胺化合物的存在得到增强。 本发明还包括用于分离含有乙烯胺化合物的核酸的反应混合物和用于实施该方法的试剂盒。
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