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    Process for the preparation in pure form of the protease activating blood clotting VII, its proenzyme or a mixture of both proteins by means of affinity chromatography
    7.
    发明授权
    Process for the preparation in pure form of the protease activating blood clotting VII, its proenzyme or a mixture of both proteins by means of affinity chromatography 失效
    以纯形式的蛋白酶活化凝血因子，其酶原或两种蛋白质的混合物通过亲和色谱法制备的方法

    公开(公告)号：US06670455B1

    公开(公告)日：2003-12-30

    申请号：US09632974

    申请日：2000-08-04

    申请人： Juergen Roemisch ,  Annette Feussner ,  Hans-Arnold Stoehr

    发明人： Juergen Roemisch ,  Annette Feussner ,  Hans-Arnold Stoehr

    IPC分类号： A61K3514

    CPC分类号： B01D15/3804 ,  B01D15/1871 ,  B01D15/327 ,  B01D15/3809 ,  B01D15/3828 ,  C12N9/6424

    摘要： A process for the preparation in pure form of the protease activating blood clotting factor VII and/or its proenzyme by the use of a chromatography separation processes and/or fractional precipitation is described. The process used may include adsorption on calcium phosphate/hydroxyapatite, a hydrophobic matrix, a chelate matrix, a matrix on which heparin or a substance related to heparin, such as heparin sulfate or dextran sulfate, is immobilized, or a matrix that is coated with an immobilized monoclonal or polyclonal antibody directed against the protein to be isolated, or F(ab) or F(ab)2 fragments of antibodies directed against the protein to be isolated. A pharmaceutical preparation and a reagent are described which contain the said protease and/or its proenzyme.

    摘要翻译： 描述了通过使用色谱分离方法和/或分级沉淀以纯形式的蛋白酶活化凝血因子VII和/或其酶原制备方法。 所使用的方法可以包括对磷酸钙/羟基磷灰石的吸附，疏水基质，螯合物基质，其上固定有肝素或与肝素相关的物质如硫酸肝素或硫酸葡聚糖的基质，或涂覆有 针对待分离的蛋白质的固定化单克隆抗体或多克隆抗体，或针对要分离的蛋白质的F（ab）或F（ab）2抗体片段）。 描述了含有所述蛋白酶和/或其酶原的药物制剂和试剂。

    Process for the preparation in pure form of the protease activating blood clotting factor VII, its proenzyme or a mixture of both proteins by means of ion-exchange chromatography
    9.
    发明授权
    Process for the preparation in pure form of the protease activating blood clotting factor VII, its proenzyme or a mixture of both proteins by means of ion-exchange chromatography 失效
    通过离子交换色谱法以纯形式的蛋白酶活化血液凝固因子VII，其酶原或两种蛋白质的混合物制备方法

    公开(公告)号：US06677440B1

    公开(公告)日：2004-01-13

    申请号：US09632627

    申请日：2000-08-04

    申请人： Juergen Roemisch ,  Annette Feussner ,  Hans-Arnold Stoehr

    发明人： Juergen Roemisch ,  Annette Feussner ,  Hans-Arnold Stoehr

    IPC分类号： A61K3800

    CPC分类号： C12N9/6424 ,  A61K38/00

    摘要： A process for the preparation of a pure form of protein, wherein the protein is protease activating blood clotting factor VII, the proenzyme for the protease, or a mixture of both the-protease and the proenzyme, is described. The protein is obtained from biological fluids or through genetic engineering and is isolated by anion- and/or cation-exchange chromatography at a pH below the isoelectric point of the protein to be isolated, or by a combination of anion- or cation-exchange chromatography with a chromatography process and/or fractional precipitation at pH between 2.5 and 9.0.

    摘要翻译： 描述了制备纯形式蛋白质的方法，其中蛋白质是蛋白酶活化凝血因子VII，蛋白酶的酶原或蛋白酶和酶的混合物。 该蛋白质是从生物流体中获得的或通过基因工程获得的，并且通过阴离子和/或阳离子交换色谱法在低于要分离的蛋白质的等电点的pH下分离，或通过阴离子或阳离子交换层析 色谱法和/或在2.5至9.0之间的pH分级沉淀。