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1.发明申请Novel Hydrogenases Isolated from Thermococcus SPP., Genes Encoding the Same, and Methods for Producing Hydrogen Using Microorganisms Having the Genes 有权从热球菌SPP分离的新型氢化酶，其编码基因以及使用具有基因的微生物产生氢的方法公开(公告)号：US20100311142A1
公开(公告)日：2010-12-09
申请号：US12746090
申请日：2009-09-07
申请人： Jung Hyun Lee , Sung Gyun Kang , Hyun Sook Lee , Sang Jin Kim , Kae Kyoung Kwon , Sun Shin Cha , Jung Ho Jeon , Yona Cho , Yun Jae Kim , Seung Seop Bae , Jae Kyu Lim , In Soon Jeong
发明人： Jung Hyun Lee , Sung Gyun Kang , Hyun Sook Lee , Sang Jin Kim , Kae Kyoung Kwon , Sun Shin Cha , Jung Ho Jeon , Yona Cho , Yun Jae Kim , Seung Seop Bae , Jae Kyu Lim , In Soon Jeong
CPC分类号： C12N9/0006 , C12N9/0067 , C12P3/00 , C12R1/01 , Y02P20/132 , Y02P20/52
摘要： The present invention relates to novel hydrogenases isolated from novel hyperthermophilic strains belonging to Thermococcus spp., genes encoding the hydrogenases, and methods of producing hydrogen using strains having the genes. According to the hydrogen production methods of the invention, a large amount of hydrogen can be produced merely by culturing the strains in specific culture conditions. Thus, the methods of the invention have advantages in that they are more economic and efficient than existing hydrogen production methods and can produce hydrogen even at high temperature.
摘要翻译： 本发明涉及从属于Thermococcus spp的新型超嗜热菌株分离的新型氢化酶，编码氢化酶的基因，以及使用具有该基因的菌株产生氢的方法。 根据本发明的氢生产方法，仅通过在特定培养条件下培养菌株可以产生大量的氢。 因此，本发明的方法的优点在于它们比现有的氢气生产方法更经济和有效，并且即使在高温下也可以产生氢。
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2.发明授权Hydrogenases isolated from Thermococcus SPP., genes encoding the same, and methods for producing hydrogen using microorganisms having the genes 有权从Thermococcus SPP分离的氢化酶，编码它们的基因，以及使用具有该基因的微生物产生氢的方法公开(公告)号：US08597926B2
公开(公告)日：2013-12-03
申请号：US12746090
申请日：2009-09-07
申请人： Jung Hyun Lee , Sung Gyun Kang , Hyun Sook Lee , Sang Jin Kim , Kae Kyoung Kwon , Sun Shin Cha , Jung Ho Jeon , Yona Cho , Yun Jae Kim , Seung Seop Bae , Jae Kyu Lim , In Soon Jeong
发明人： Jung Hyun Lee , Sung Gyun Kang , Hyun Sook Lee , Sang Jin Kim , Kae Kyoung Kwon , Sun Shin Cha , Jung Ho Jeon , Yona Cho , Yun Jae Kim , Seung Seop Bae , Jae Kyu Lim , In Soon Jeong
IPC分类号： C12N9/02
CPC分类号： C12N9/0006 , C12N9/0067 , C12P3/00 , C12R1/01 , Y02P20/132 , Y02P20/52
摘要： The present invention relates to novel hydrogenases isolated from novel hyperthermophilic strains belonging to Thermococcus spp., genes encoding the hydrogenases, and methods of producing hydrogen using strains having the genes. According to the hydrogen production methods of the invention, a large amount of hydrogen can be produced merely by culturing the strains in specific culture conditions. Thus, the methods of the invention have advantages in that they are more economic and efficient than existing hydrogen production methods and can produce hydrogen even at high temperature.
摘要翻译： 本发明涉及从属于Thermococcus spp的新型超嗜热菌株分离的新型氢化酶，编码氢化酶的基因，以及使用具有该基因的菌株产生氢的方法。 根据本发明的氢生产方法，仅通过在特定培养条件下培养菌株可以产生大量的氢。 因此，本发明的方法的优点在于它们比现有的氢气生产方法更经济和有效，并且即使在高温下也可以产生氢。
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公开(公告)号：US20090148896A1
公开(公告)日：2009-06-11
申请号：US12089587
申请日：2006-10-02
申请人： Jung Hyun Lee , Suk Tae Kwon , Sung Gyun Kang , Sang Jin Kim , Jung Ho Hyun , Kae Kyoung Kwon , Yun Jae Kim , Hyun Sook Lee , Seung Seob Bae , Ki Hoon Nam , Jae Kyu Lim , Jung Ho Jeon , Sung Hyun Yang
发明人： Jung Hyun Lee , Suk Tae Kwon , Sung Gyun Kang , Sang Jin Kim , Jung Ho Hyun , Kae Kyoung Kwon , Yun Jae Kim , Hyun Sook Lee , Seung Seob Bae , Ki Hoon Nam , Jae Kyu Lim , Jung Ho Jeon , Sung Hyun Yang
CPC分类号： C12N9/1252
摘要： The present invention relates to a hyperthermophilic DNA polymerase and a preparation method thereof. The invention provides a novel hyperthermophilic DNA polymerase isolated from a Thermococcus sp. strain, a functional equivalent thereof, a protein having the amino acid sequence thereof, and a preparation method thereof. The DNA polymerase according to the invention is a DNA polymerase, which is hyperthermophilic and has an elongation ability and fidelity higher than those of prior commercial DNA polymerases. Thus, the DNA polymerase according to the invention will be useful in precision analysis, precision diagnosis, identification and the like, which require accurate PCR.
摘要翻译： 本发明涉及一种超嗜热DNA聚合酶及其制备方法。 本发明提供了从Thermococcus sp。分离的新型超嗜热DNA聚合酶。 菌株，其功能等同物，具有其氨基酸序列的蛋白质及其制备方法。 根据本发明的DNA聚合酶是DNA聚合酶，其是超嗜热的，并且具有比以前的商业DNA聚合酶更高的伸长能力和保真度。 因此，根据本发明的DNA聚合酶可用于需要精确PCR的精密分析，精确诊断，鉴定等中。
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公开(公告)号：US08257953B2
公开(公告)日：2012-09-04
申请号：US12089587
申请日：2006-10-02
申请人： Jung Hyun Lee , Suk Tae Kwon , Sung Gyun Kang , Sang Jin Kim , Jung Ho Hyun , Kae Kyoung Kwon , Yun Jae Kim , Hyun Sook Lee , Seung Seob Bae , Ki Hoon Nam , Jae Kyu Lim , Jung Ho Jeon , Sung Hyun Yang
发明人： Jung Hyun Lee , Suk Tae Kwon , Sung Gyun Kang , Sang Jin Kim , Jung Ho Hyun , Kae Kyoung Kwon , Yun Jae Kim , Hyun Sook Lee , Seung Seob Bae , Ki Hoon Nam , Jae Kyu Lim , Jung Ho Jeon , Sung Hyun Yang
CPC分类号： C12N9/1252
摘要： The present invention relates to a hyperthermophilic DNA polymerase and a preparation method thereof. The invention provides a novel hyperthermophilic DNA polymerase isolated from a Thermococcus sp. strain, a functional equivalent thereof, a protein having the amino acid sequence thereof, and a preparation method thereof. The DNA polymerase according to the invention is a DNA polymerase, which is hyperthermophilic and has an elongation ability and fidelity higher than those of prior commercial DNA polymerases. Thus, the DNA polymerase according to the invention will be useful in precision analysis, precision diagnosis, identification and the like, which require accurate PCR.
摘要翻译： 本发明涉及一种超嗜热DNA聚合酶及其制备方法。 本发明提供了从Thermococcus sp。分离的新型超嗜热DNA聚合酶。 菌株，其功能等同物，具有其氨基酸序列的蛋白质及其制备方法。 根据本发明的DNA聚合酶是DNA聚合酶，其是超嗜热的，并且具有比以前的商业DNA聚合酶更高的伸长能力和保真度。 因此，根据本发明的DNA聚合酶可用于需要精确PCR的精密分析，精确诊断，鉴定等。
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公开(公告)号：US20100297706A1
公开(公告)日：2010-11-25
申请号：US12525347
申请日：2007-10-02
申请人： Jung Hyun Lee , Sung Gyun Kang , Sang Jin Kim , Kae Kyoung Kwon , Hyun Sook Lee , Yun Jae Kim , Yong Gu Ryu , Seung Seob Bae , Jae Kyu Lim , Jung Ho Jeon , Yo Na Cho
发明人： Jung Hyun Lee , Sung Gyun Kang , Sang Jin Kim , Kae Kyoung Kwon , Hyun Sook Lee , Yun Jae Kim , Yong Gu Ryu , Seung Seob Bae , Jae Kyu Lim , Jung Ho Jeon , Yo Na Cho
CPC分类号： C12N9/1252
摘要： The present invention relates to mutant DNA polymerases and their genes isolated from Thermococcus sp. More specifically, the present invention relates to mutant DNA polymerases which are originally isolated from Thermococcus sp NA1. strain and produced by site-specific mutagenesis, their amino acid sequences, genes encoding said mutant DNA polymerases, their nucleic acids sequences, recombinant vectors containing said nucleic acids sequences, host cells transformed with thereof and methods for producing mutant DNA polymerase protein by using thereof. As mutant DNA polymerases according to the present invention have increased processivity by site-specific mutagenesis on exonuclease active site compared to wild type DNA polymerase, the present invention is broadly applicable for PCR in various molecular genetic technologies.
摘要翻译： 本发明涉及从Thermococcus sp。分离的突变型DNA聚合酶及其基因。 更具体地，本发明涉及最初从Thermococcus sp NA1分离的突变型DNA聚合酶。 菌株并通过位点特异性诱变产生，其氨基酸序列，编码所述突变型DNA聚合酶的基因，其核酸序列，含有所述核酸序列的重组载体，用其转化的宿主细胞和通过使用其产生突变型DNA聚合酶蛋白的方法 。 由于本发明的突变型DNA聚合酶与野生型DNA聚合酶相比，通过对外切核酸酶活性位点的位点特异性诱变具有增加的进行性，因此本发明广泛适用于各种分子遗传技术中的PCR。
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公开(公告)号：US20110020896A1
公开(公告)日：2011-01-27
申请号：US12444015
申请日：2007-10-02
申请人： Jung Hyun Lee , Sung Gyun Kang , Sang Jin Kim , Kae Kyoung Kwon , Hyun Sook Lee , Yun Jae Kim , Seung Seob Bae , Jae Kyu Lim , Jung Ho Jeon , Yo Na Cho , Suk Tae Kwon
发明人： Jung Hyun Lee , Sung Gyun Kang , Sang Jin Kim , Kae Kyoung Kwon , Hyun Sook Lee , Yun Jae Kim , Seung Seob Bae , Jae Kyu Lim , Jung Ho Jeon , Yo Na Cho , Suk Tae Kwon
CPC分类号： C12N9/1252
摘要： The present invention relates to mutant DNA polymerases, their genes and their uses. More specifically, the present invention relates to mutant DNA polymerases which are originally isolated from Thermococcus sp NA1. strain and produced by site-specific mutagenesis, their amino acid sequences, genes encoding said mutant DNA polymerases, their nucleic acids and PCR methods by using thereof. As mutant DNA polymerases according to the present invention have decreased proofreading activity and changed function of inosine sensing effectively compared to wild type DNA polymerase, PCR using primers with specific nucleic acids has made rapid progress. Therefore, the present invention is broadly applicable for PCR in various molecular genetic technologies.
摘要翻译： 本发明涉及突变型DNA聚合酶，其基因及其用途。 更具体地，本发明涉及最初从Thermococcus sp NA1分离的突变型DNA聚合酶。 菌株并通过位点特异性诱变产生，其氨基酸序列，编码所述突变型DNA聚合酶的基因，其核酸和通过其使用的PCR方法。 由于与野生型DNA聚合酶相比，根据本发明的突变型DNA聚合酶与野生型DNA聚合酶相比，具有降低的校正活性和肌苷感应功能的改变，所以使用具有特异性核酸的引物的PCR已经取得了快速进展。 因此，本发明广泛适用于各种分子遗传技术中的PCR。
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