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    Method for qualitative and quantitative detection of common wheat
    2.
    发明授权
    Method for qualitative and quantitative detection of common wheat 有权
    普通小麦定性和定量检测方法

    公开(公告)号：US08865433B2

    公开(公告)日：2014-10-21

    申请号：US13518298

    申请日：2010-12-17

    申请人： Kazumi Kitta ,  Satoshi Furui ,  Junichi Mano ,  Yasuyuki Matsuoka ,  Shinichiro Arami ,  Megumi Sato ,  Hiroyuki Haraguchi ,  Youichi Kurimoto ,  Shinjiro Imai ,  Keiko Tanaka

    发明人： Kazumi Kitta ,  Satoshi Furui ,  Junichi Mano ,  Yasuyuki Matsuoka ,  Shinichiro Arami ,  Megumi Sato ,  Hiroyuki Haraguchi ,  Youichi Kurimoto ,  Shinjiro Imai ,  Keiko Tanaka

    IPC分类号： C12P19/34 ,  C12Q1/68

    CPC分类号： C12Q1/6895

    摘要： Disclosed are: a method for detecting common wheat among from wheat varieties contained in a sample of interest such as a food raw material or a processed food specifically, with high sensitivity, and in a qualitative and/or quantitative manner; a method for discriminating between common wheat and a wheat variety other than common wheat (e.g., durum wheat) contained in a food raw material or a processed food and detecting the common wheat in a qualitative and/or quantitative manner; and a primer set, a nucleic acid probe, and a detection kit, each of which can be used in the methods employing a PCR method. Specifically disclosed are: a method for detecting the occurrence of common wheat in a sample of interest, which comprises carrying out a PCR method using a nucleic acid extracted from the sample as a template and using a primer comprising the nucleotide sequence represented by SEQ ID NO:5 and a primer comprising the nucleotide sequence represented by SEQ ID NO:6 and detecting the occurrence of a PCR amplification product; and a method for detecting the occurrence of common wheat in a sample of interest, which comprises carrying out a quantitative PCR method using a nucleic acid extracted from the sample as a template and using a primer comprising the nucleotide sequence represented by SEQ ID NO:5, a primer comprising the nucleotide sequence represented by SEQ ID NO:6 and a nucleic acid probe comprising the nucleotide sequence represented by SEQ ID NO:11 and detecting the occurrence of common wheat qualitatively and/or quantitatively.

    摘要翻译： 本发明公开了一种以高灵敏度，定性和/或定量方式特异性检测食品原料或加工食品中所含小麦品种中普通小麦的方法; 用于区分普通小麦和食品原料或加工食品中包含的普通小麦（例如硬粒小麦）以外的小麦品种的方法，并以定性和/或定量方式检测普通小麦; 以及引物组，核酸探针和检测试剂盒，其可以用于采用PCR方法的方法中。 具体公开的是：检测感兴趣的样品中普通小麦的发生的方法，包括使用从样品提取的核酸作为模板进行PCR方法，并使用包含SEQ ID NO所示的核苷酸序列的引物 5和包含SEQ ID NO：6所示核苷酸序列的引物，并检测PCR扩增产物的出现; 以及用于检测感兴趣样品中普通小麦的发生的方法，其包括使用从样品提取的核酸作为模板进行定量PCR方法，并使用包含SEQ ID NO：5所示核苷酸序列的引物 ，包含由SEQ ID NO：6表示的核苷酸序列的引物和包含SEQ ID NO：11所示的核苷酸序列的核酸探针，并定性和/或定量地检测普通小麦的发生。

    Method for producing hot-rolled steel sheets having a low yield ratio and a high tensile strength due to dual phase structure
    4.
    发明授权
    Method for producing hot-rolled steel sheets having a low yield ratio and a high tensile strength due to dual phase structure 失效
    用于生产由于双相结构而具有低屈服比和高拉伸强度的热轧钢板的方法

    公开(公告)号：US4502897A

    公开(公告)日：1985-03-05

    申请号：US438844

    申请日：1982-10-15

    申请人： Masahiko Morita ,  Junichi Mano ,  Minoru Nishida ,  Tomoo Tanaka ,  Nobuo Aoyagi ,  Syoichi Takizawa

    发明人： Masahiko Morita ,  Junichi Mano ,  Minoru Nishida ,  Tomoo Tanaka ,  Nobuo Aoyagi ,  Syoichi Takizawa

    IPC分类号： C22C38/00 ,  C21D1/19 ,  C21D8/02 ,  C21D9/46 ,  C22C38/18 ,  C22C38/38

    CPC分类号： C21D1/19 ,  C21D8/0226 ,  C22C38/18 ,  C22C38/38 ,  C21D2211/005 ,  C21D2211/008 ,  C21D8/0263

    摘要： The present invention aims to obtain C-Si-Mn-Cr system of hot-rolled dual phase structured steel sheets having a low yield ratio YR of not greater than 65%, an excellent strength-elongation balance M, a low variation in quality and an excellent cold formability through stepwise cooling regulation in the course of cooling from the final rolling to coiling.The present invention is a method for producing hot-rolled steel sheets having a low yield ratio and a high tensile strength due to dual phase structure by effecting the final rolling of a hot-rolled steel sheet containing 0.02-0.2% of C, 0.05-2.0% of Si, 0.5-2.0% of Mn and 0.3-1.5% of Cr as the essential components at a temperature of finishing the final rolling of 780.degree. C., rapidly cooling the thus treated steel sheet at a cooling rate of more than 40.degree. C./S to the temperature range wherein the transformation of .gamma. into .alpha. is efficiently caused corresponding to the components in the steel and the rolling hysteresis, holding the steel sheet at this temperature range for more than 5 seconds and rapidly cooling the thus treated steel sheet at a cooling rate of more than 50.degree. C./S from said held temperature to a temperature of 550.degree.-200.degree. C. to obtain a hot-rolled steel sheet having YR of not greater than 65%, M of not less than 60 and a low variation of quality and an excellent cold formability.

    摘要翻译： PCT No.PCT / JP82 / 00030 Sec。 371日期1982年10月15日 102（e）日期1982年10月15日PCT提交1982年2月2日PCT公布。 公开号WO82 / 02902 日本1982年9月2日。本发明旨在获得低收率比YR为65％以下的热轧双相结构钢板的C-Si-Mn-Cr系，优异的强度伸长率平衡M ，通过从最终轧制到卷取的冷却过程中的逐步冷却调节，质量的差异和优异的冷成形性。 本发明是通过对含有0.02-0.2％C，0.05〜0.2％的热轧钢板进行最终轧制，由于双相结构而制造具有低屈服比和高拉伸强度的热轧钢板的方法， 在2.0％的Si，0.5-2.0％的Mn和0.3-1.5％的Cr作为必要组分，在最终轧制的最终轧制温度为780℃，快速冷却如此处理的钢板以大于 40℃/秒到对应于钢中的成分和轧制滞后有效地将γ转变成α的温度范围，将钢板保持在该温度范围超过5秒并迅速冷却 将处理后的钢板以大于50℃/秒的冷却速度从所述保持温度升温至550〜-200℃，得到YR为65％以下的热轧钢板，M 不低于60，质量变化不大， 优良的冷成型性。

    METHOD FOR QUALITATIVE AND QUANTITATIVE DETECTION OF COMMON WHEAT
    6.
    发明申请
    METHOD FOR QUALITATIVE AND QUANTITATIVE DETECTION OF COMMON WHEAT 有权
    普通小麦定性和定量检测方法

    公开(公告)号：US20120264128A1

    公开(公告)日：2012-10-18

    申请号：US13518298

    申请日：2010-12-17

    申请人： Kazumi Kitta ,  Satoshi Furui ,  Junichi Mano ,  Yasuyuki Matsuoka ,  Shinichiro Arami ,  Megumi Sato ,  Hiroyuki Haraguchi ,  Youichi Kurimoto ,  Shinjiro Imai ,  Keiko Tanaka

    发明人： Kazumi Kitta ,  Satoshi Furui ,  Junichi Mano ,  Yasuyuki Matsuoka ,  Shinichiro Arami ,  Megumi Sato ,  Hiroyuki Haraguchi ,  Youichi Kurimoto ,  Shinjiro Imai ,  Keiko Tanaka

    IPC分类号： G01N21/64 ,  C07H21/04 ,  C12Q1/68

    CPC分类号： C12Q1/6895

    摘要： Disclosed are: a method for detecting common wheat among from wheat varieties contained in a sample of interest such as a food raw material or a processed food specifically, with high sensitivity, and in a qualitative and/or quantitative manner; a method for discriminating between common wheat and a wheat variety other than common wheat (e.g., durum wheat) contained in a food raw material or a processed food and detecting the common wheat in a qualitative and/or quantitative manner; and a primer set, a nucleic acid probe, and a detection kit, each of which can be used in the methods employing a PCR method. Specifically disclosed are: a method for detecting the occurrence of common wheat in a sample of interest, which comprises carrying out a PCR method using a nucleic acid extracted from the sample as a template and using a primer comprising the nucleotide sequence represented by SEQ ID NO:5 and a primer comprising the nucleotide sequence represented by SEQ ID NO:6 and detecting the occurrence of a PCR amplification product; and a method for detecting the occurrence of common wheat in a sample of interest, which comprises carrying out a quantitative PCR method using a nucleic acid extracted from the sample as a template and using a primer comprising the nucleotide sequence represented by SEQ ID NO:5, a primer comprising the nucleotide sequence represented by SEQ ID NO:6 and a nucleic acid probe comprising the nucleotide sequence represented by SEQ ID NO:11 and detecting the occurrence of common wheat qualitatively and/or quantitatively.

    摘要翻译： 本发明公开了一种以高灵敏度，定性和/或定量方式特异性检测食品原料或加工食品中所含小麦品种中普通小麦的方法; 用于区分普通小麦和食品原料或加工食品中包含的普通小麦（例如硬粒小麦）以外的小麦品种的方法，并以定性和/或定量方式检测普通小麦; 以及引物组，核酸探针和检测试剂盒，其可以用于采用PCR方法的方法中。 具体公开的是：检测感兴趣的样品中普通小麦的发生的方法，包括使用从样品提取的核酸作为模板进行PCR方法，并使用包含SEQ ID NO所示的核苷酸序列的引物 5和包含SEQ ID NO：6所示核苷酸序列的引物，并检测PCR扩增产物的出现; 以及用于检测感兴趣样品中普通小麦的发生的方法，其包括使用从样品提取的核酸作为模板进行定量PCR方法，并使用包含SEQ ID NO：5所示核苷酸序列的引物 ，包含由SEQ ID NO：6表示的核苷酸序列的引物和包含SEQ ID NO：11所示的核苷酸序列的核酸探针，并定性和/或定量地检测普通小麦的发生。