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    Fluorescent substances
    1.
    发明授权
    Fluorescent substances 失效
    荧光物质

    公开(公告)号：US06642375B2

    公开(公告)日：2003-11-04

    申请号：US09731279

    申请日：2000-12-06

    申请人： Hiroko Inomata ,  Hiroshi Shinoki ,  Masayoshi Kojima ,  Yukio Sudo ,  Junji Nishigaki ,  Osamu Seshimoto

    发明人： Hiroko Inomata ,  Hiroshi Shinoki ,  Masayoshi Kojima ,  Yukio Sudo ,  Junji Nishigaki ,  Osamu Seshimoto

    IPC分类号： C07H1904

    CPC分类号： G01N33/582

    摘要： The present invention provides a fluorescent substance which is represented by a formula: A-B-C wherein A is a residue of natural or synthetic nucleotide, oligonucleotide, polynucleotide, or derivative thereof, and binds to B at a base moiety in said residue, or A is a residue of avidin or streptavidin; B is a divalent linking group or a single bond; and C is a monovalent group derived from a general formula (I) and binds to B at a reactive group present in R1 or R2: wherein R1 and R2 each independently represent an alkyl group that may be substituted with a reactive group capable of covalently bonding to A-B-; R3, R4, R5, and R6 each independently represent an alkyl group, and R3 and R4, and/or R5 and R6 may bind to each other to form a saturated carbon-ring together with a carbon atom(s) to which they bind; V1, V2, V3, V4, V5, V6, V7, V8, V9 and V10 each independently represent a hydrogen atom or a monovalent substituent, and two adjacent groups thereof may bind to form a ring; L1, L2, and L3 represent a substituted or unsubstituted methine group; each of m, n, s, and t represents 0 or 1, provided that m+n=1 and s+t=1; p represents 1, 2, or 3; M represents a counter ion; and q represents a number required to neutralize the charge of a molecule. The fluorescent substance of the present invention is useful as a labeling substance for nucleic acids, or as a reagent for analyzing biological components such as nucleic acids, proteins or sugars.

    摘要翻译： 本发明提供一种荧光物质，其由下式表示：AB-C，其中A是天然或合成的核苷酸，寡核苷酸，多核苷酸或其衍生物的残基，并与所述残基中的碱基部分的B结合，或A是 抗生物素蛋白或链霉亲和素的残基; B是二价连接基团或单键; 和C是衍生自通式（I）的一价基团，并且在R 1或R 2中存在的反应性基团上与B结合：其中R 1和R 2各自独立地表示烷基， 可以被能够与AB-共价结合的反应性基团取代; R 3，R 4，R 5和R 6各自独立地表示烷基，R 3和R 4和/或R 5和R 6独立地表示烷基， 可以彼此结合形成饱和碳环与它们所结合的碳原子一起; V 1，V 2，V 3，V 4，V 5，V 6，V 7，V 8，V 9和V 10 独立地表示氢原子或一价取代基，其两个相邻基团可以结合形成环; L 1，L 2和L 3表示取代或未取代的次甲基; m，n，s和t中的每一个表示0或1，条件是m + n = 1且s + t = 1; p表示1,2或3; M表示抗衡离子; q表示中和分子电荷所需的数。 本发明的荧光物质可用作核酸的标记物质，或用作分析核酸，蛋白质或糖类等生物成分的试剂。

    Fixation of nucleotide derivatives to solid carrier
    3.
    发明授权
    Fixation of nucleotide derivatives to solid carrier 有权
    核苷酸衍生物固定于固体载体

    公开(公告)号：US06664051B1

    公开(公告)日：2003-12-16

    申请号：US09706405

    申请日：2000-11-03

    申请人： Hiroshi Shinoki ,  Yukio Sudo ,  Osamu Seshimoto

    发明人： Hiroshi Shinoki ,  Yukio Sudo ,  Osamu Seshimoto

    IPC分类号： C07H2104

    CPC分类号： C40B40/06 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00677 ,  B82Y30/00 ,  C40B50/06

    摘要： A DNA chip or its analogue composed of nucleotide derivatives attached to a solid carrier is prepared by method of bringing nucleotide derivatives having a reactive group at each one terminal into contact with a solid carrier having thereon reactive groups in an aqueous phase in the presence of a transferase which is capable of producing a covalent bond by rearrangement of the reactive group of the nucleotide derivative and the reactive group of the solid carrier.

    摘要翻译： 通过以下方法制备DNA芯片或其由连接于固体载体上的核苷酸衍生物组成的类似物的方法：使得在每个末端具有反应性基团的核苷酸衍生物与其中具有反应性基团的固体载体在水相中存在下接触 能够通过核苷酸衍生物的反应性基团和固体载体的反应性基团的重排而产生共价键的转移酶。

    Assay chip
    6.
    发明申请
    Assay chip 审中-公开
    测定芯片

    公开(公告)号：US20070178521A1

    公开(公告)日：2007-08-02

    申请号：US10592748

    申请日：2005-03-15

    申请人： Yoshiki Sakaino ,  Yukio Sudo ,  Yoshihiko Abe ,  Bo Yang

    发明人： Yoshiki Sakaino ,  Yukio Sudo ,  Yoshihiko Abe ,  Bo Yang

    IPC分类号： G01N33/53 ,  C12M3/00

    CPC分类号： B01L3/502753 ,  B01L3/5023 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2400/0409

    摘要： An integrated assay chip for assaying one or more components of a specimen, which comprises: (1) one or more pretreatment elements for pretreating a specimen; (2) one or more multilayer dry assay elements capable of assaying one or more components of the pretreated specimen; and (3) one or more flow channels connecting the pretreatment element and the multilayer dry assay element is provided.

    摘要翻译： 一种用于测定样品的一种或多种组分的综合测定芯片，其包括：（1）用于预处理样品的一种或多种预处理元件; （2）能够测定预处理样品的一种或多种组分的一种或多种多层干测定元件; 和（3）提供连接预处理元件和多层干测定元件的一个或多个流动通道。

    Microreactor
    7.
    发明申请
    Microreactor 审中-公开
    微反应器

    公开(公告)号：US20070141616A1

    公开(公告)日：2007-06-21

    申请号：US11657538

    申请日：2007-01-25

    申请人： Yukio Sudo

    发明人： Yukio Sudo

    IPC分类号： C12Q1/68 ,  G01N33/53 ,  C12M1/34

    CPC分类号： G01N33/558

    摘要： The present invention provides a reactive microreactor wherein a group of the following formula (1) which can bind to a member of specifically binding partner, is bound to a part or entirety of a wall surface of a channel: -L-SO2—X1  (1) wherein X1 represents —CR1═CR2R3 or —CHR1—CR2R3Y; R1, R2 and R3 independently represent an atom or a group selected from the group consisting of a hydrogen atom, a C1-6 alkyl group, a C6-20 aryl group, and a C7-20 aralkyl group having a C1-6 alkyl chain; Y represents a group which can be substitutable by a nucleophilic reagent or a group which is released as “HY” by a base; and L represents a linking group. The microreactor according to the present invention has advantages that it requires a less amount of a reagent and achieves a higher S/N ratio.

    摘要翻译： 本发明提供了一种反应性微反应器，其中可结合特异性结合配偶体成员的下列通式（1）的基团与通道的壁表面的一部分或全部结合： 描述=“在线公式”end =“lead”？> - L-SO <2> <1> <1> <？in-line-formula description =“ 在线公式“end =”tail“？>其中X 1表示-CR 1 -CR 2 2 R 3， SUP>或-CHR 1 -CR 2 R 3 3 Y; R 1，R 2和R 3独立地表示选自以下的原子或基团：氢原子，C 1-6 烷基，C6-20芳基和具有C1-6烷基链的C7-20芳烷基; Y表示可以被亲核试剂或被基团释放为“HY”的基团取代的基团; L表示连接基团。 根据本发明的微反应器具有需要较少量的试剂并实现更高S / N比的优点。

    Analytical element for immunoassay and method for its preparation
    9.
    依法登记的发明
    Analytical element for immunoassay and method for its preparation 失效
    免疫测定的分析元素及其制备方法

    公开(公告)号：USH1664H

    公开(公告)日：1997-07-01

    申请号：US320771

    申请日：1989-03-08

    申请人： Yukio Sudo ,  Nobuhito Masuda

    发明人： Yukio Sudo ,  Nobuhito Masuda

    IPC分类号： G01N33/543

    CPC分类号： G01N33/54386

    摘要： A dry-type analytical element for immunoassay utilizing antigen-antibody reaction in which the antigenic component is isolated from the antibody component by incorporating both components in separate layers or separately in a layer using different solvents. The analytical element is excellent in sensitivity and accuracy, and the analytical operation is simple.

    摘要翻译： 用于使用抗原 - 抗体反应进行免疫测定的干式分析元件，其中通过将两种组分并入分开的层中，或者使用不同溶剂在一层中分别地将抗原成分从抗体成分中分离出来。 分析元件的灵敏度和精度都很好，分析操作简单。