公开(公告)号：US20230082473A1

公开(公告)日：2023-03-16

申请号：US17801715

申请日：2021-02-19

Applicant: KANEKA CORPORATION

Inventor： Fuminori KONOIKE ,  Kazunobu MINAKUCHI ,  Dai MURATA ,  Kenta KISHI ,  Masakatsu NISHIHACHIJO

IPC: B01J20/04 ,  C12N1/08 ,  C12N7/00 ,  C07K1/22 ,  B01D39/16 ,  B01D39/20

Abstract: The objective of the present invention is to provide a method for effectively and easily reducing an amount of a specific impurity in a liquid. The method for reducing an amount of a nucleic acid in a liquid according to the present invention is characterized in comprising the steps of contacting the liquid with a water-insoluble magnesium compound to adsorb at least a part of the nucleic acid on the water-insoluble magnesium compound. Also, the objective of the present invention is to provide an adsorbing filter useful for purifying a useful substance, such as an antibody and an antibody-like molecule, used as a purification material for effectively removing an impurity with easily maintaining the yield of the target substance due to excellent adsorption ability to a nucleic acid and low adsorption ability to an antibody, an antibody-like molecule or the like. The adsorbing filter is characterized in comprising the layer comprising a water-insoluble magnesium compound.

公开(公告)号：US20180044401A1

公开(公告)日：2018-02-15

申请号：US15501320

申请日：2015-08-27

Applicant: KANEKA CORPORATION

Inventor： Shinichi YOSHIDA ,  Dai MURATA

IPC: C07K14/735

CPC classification number: C07K14/70535 ,  C07K16/00 ,  C07K2317/55

Abstract: The objective of the present invention is to provide a peptide of which both of binding forces to a Fc region and a Fab region are superior. In addition, the objective of the present invention is to provide a DNA which encodes the peptide, a vector which contains the DNA, and a transformant which is transformed by the vector. The problem can be solved by providing the peptide having the specific sequence.

公开(公告)号：US20220135705A1

公开(公告)日：2022-05-05

申请号：US17434039

申请日：2020-02-27

Applicant: KANEKA CORPORATION

Inventor： Takuma SUZUKI ,  Dai MURATA ,  Masakatsu NISHIHACHIJYO ,  Hisako YAURA

IPC: C07K17/14 ,  C07K1/22

Abstract: The objective of the present invention is to provide a method for efficiently supporting a thiol group-including compound on an insoluble base material. The method for supporting a thiol group-including compound on an insoluble base material according to the present invention is characterized in comprising Step A: treating the thiol group-including compound with a thiol group-including organic reducing agent and an inorganic reducing agent, and Step B: contacting a reaction liquid of said Step A with the insoluble base material.

公开(公告)号：US20170334947A1

公开(公告)日：2017-11-23

申请号：US15506657

申请日：2015-08-27

Applicant: KANEKA CORPORATION

Inventor： Dai MURATA ,  Shinichi YOSHIDA

IPC: C07K1/22 ,  B01D15/38 ,  C07K17/06 ,  C07K16/00 ,  C07K14/315

CPC classification number: C07K1/22 ,  B01D15/3809 ,  C07B2200/11 ,  C07K14/315 ,  C07K16/00 ,  C07K17/00 ,  C07K17/04 ,  C07K17/06 ,  C07K17/12 ,  C07K17/14 ,  C07K2317/55

Abstract: The objective of the present invention is to provide an affinity separation matrix having excellent adsorption performance and binding capacity to a peptide containing a Fab region of IgG, and a method for producing a Fab region-containing peptide using the affinity separation matrix. The affinity separation matrix according to the present invention is characterized in that a Fab region-binding peptide is immobilized as a ligand on a water-insoluble carrier in a density of 1.0 mg/mL-gel or more.

公开(公告)号：US20160289306A1

公开(公告)日：2016-10-06

申请号：US14914439

申请日：2014-08-28

Applicant: KANEKA CORPORATION

Inventor： Shinichi YOSHIDA ,  Dai MURATA

IPC: C07K16/12 ,  C07K14/315 ,  C07K1/22

CPC classification number: C07K16/1275 ,  C07K1/22 ,  C07K14/315 ,  C07K2317/55 ,  C12N2510/02

Abstract: An object of the present invention is to provide a Fab region-binding peptide having an excellent ability for binding to a Fab region of IgG, an affinity separation matrix having the peptide as a ligand, and a method for producing a Fab region-containing protein, the method using the affinity separation matrix. Further, another object of the present invention is to provide a DNA encoding for the peptide, a vector containing the DNA, and a transformant which has been transformed by the vector. The Fab region-binding peptide according to the present invention is characterized in having a mutation at a specific site in comparison with wild-type SpG-β1.

Abstract translation: 本发明的目的是提供具有优异的结合IgG Fab区域的Fab区域结合肽，具有该肽作为配体的亲和力分离基质，以及制备含有Fab区域的蛋白质的方法 ，使用亲和力分离矩阵的方法。 此外，本发明的另一个目的是提供编码肽的DNA，含有DNA的载体和已经通过载体转化的转化体。 与野生型SpG-β1相比，本发明的Fab区结合肽的特征在于在特定部位具有突变。