公开(公告)号：US20160244483A1

公开(公告)日：2016-08-25

申请号：US15025152

申请日：2014-09-26

Applicant: KANEKA CORPORATION

Inventor： Takahiro OKUBO ,  Yoshikazu KAWAI ,  Masaru HIRANO ,  Fuminori KONOIKE ,  Keiichi KARASUGI ,  Tatsuya HONDA

IPC: C07K1/22 ,  B01J20/26 ,  C08J9/00 ,  B01J20/285 ,  B01J20/30 ,  C07K16/00 ,  B01J20/24 ,  B01J20/28

CPC classification number: C07K1/22 ,  B01D15/3809 ,  B01J20/24 ,  B01J20/267 ,  B01J20/28004 ,  B01J20/28016 ,  B01J20/285 ,  B01J20/286 ,  B01J20/3071 ,  B01J20/3085 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3274 ,  B01J20/3293 ,  B01J2220/52 ,  C07K16/00 ,  C08B1/08 ,  C08B3/06 ,  C08B15/005 ,  C08B15/08 ,  C08B15/10 ,  C08B16/00 ,  C08J3/16 ,  C08J9/00 ,  C08J2301/02 ,  C08J2301/04 ,  C08J2301/12 ,  C08L1/02 ,  C08L1/04 ,  C08L1/12 ,  C08L2205/18

Abstract: A process for producing porous cellulose beads of the present invention is characterized by comprising the steps of a) mixing an alkali aqueous solution and cellulose to prepare cellulose micro dispersion at low temperature, b) adding water to the cellulose micro dispersion to prepare cellulose slurry, and d) bringing the cellulose slurry into contact with coagulation solvent. A carrier for ligand immobilization of the present invention is characterized by being by shrinking polysaccharide porous beads not less than 10% by a shrinkage rate defined by the following formula, and crosslinking the polysaccharide porous beads: Shrinkage rate (%)=(1−V2/V1)×100 (wherein, V1 indicates the gel volume of polysaccharide porous beads before shrinkage, and V2 indicates the gel volume of polysaccharide porous beads after shrinkage).

Abstract translation: 本发明的多孔纤维素珠粒的制造方法的特征在于，包括以下步骤：a）将碱水溶液和纤维素混合以在低温下制备纤维素微分散体，b）向纤维素微分散体中加入水以制备纤维素浆料， 和d）使纤维素浆料与凝固溶剂接触。 本发明的配位体固定用载体的特征在于通过以下式定义的收缩率收缩不少于10％的多糖多孔珠，并交联多糖多孔珠：收缩率（％）=（1-V2 / V1）×100（其中，V1表示收缩前的多糖多孔珠粒的凝胶体积，V2表示收缩后的多糖多孔珠粒的凝胶体积）。

公开(公告)号：US20190256555A1

公开(公告)日：2019-08-22

申请号：US16227688

申请日：2018-12-20

Applicant: KANEKA CORPORATION

Inventor： Takahiro OKUBO ,  Yoshikazu KAWAI ,  Masaru HIRANO ,  Fuminori KONOIKE ,  Keiichi KARASUGI ,  Tatsuya HONDA

IPC: C07K1/22 ,  C08J9/00 ,  B01J20/24 ,  B01J20/30 ,  C08B1/08 ,  C08B15/08 ,  C08B15/10 ,  C08B3/06 ,  C07K16/00 ,  B01J20/26 ,  B01J20/285 ,  C08J3/16 ,  B01J20/28 ,  B01J20/32 ,  B01J20/286 ,  B01D15/38 ,  C08L1/12 ,  C08L1/04 ,  C08L1/02 ,  C08B16/00 ,  C08B15/00

Abstract: A carrier for ligand immobilization obtained by shrinking polysaccharide porous beads not less than 10% by a shrinkage rate defined by the following formula, and crosslinking the polysaccharide porous beads: Shrinkage rate (%)=(1−V2/V1)×100 (wherein, V1 indicates the gel volume of polysaccharide porous beads before shrinkage, and V2 indicates the gel volume of polysaccharide porous beads after shrinkage).

公开(公告)号：US20230082473A1

公开(公告)日：2023-03-16

申请号：US17801715

申请日：2021-02-19

Applicant: KANEKA CORPORATION

Inventor： Fuminori KONOIKE ,  Kazunobu MINAKUCHI ,  Dai MURATA ,  Kenta KISHI ,  Masakatsu NISHIHACHIJO

IPC: B01J20/04 ,  C12N1/08 ,  C12N7/00 ,  C07K1/22 ,  B01D39/16 ,  B01D39/20

Abstract: The objective of the present invention is to provide a method for effectively and easily reducing an amount of a specific impurity in a liquid. The method for reducing an amount of a nucleic acid in a liquid according to the present invention is characterized in comprising the steps of contacting the liquid with a water-insoluble magnesium compound to adsorb at least a part of the nucleic acid on the water-insoluble magnesium compound. Also, the objective of the present invention is to provide an adsorbing filter useful for purifying a useful substance, such as an antibody and an antibody-like molecule, used as a purification material for effectively removing an impurity with easily maintaining the yield of the target substance due to excellent adsorption ability to a nucleic acid and low adsorption ability to an antibody, an antibody-like molecule or the like. The adsorbing filter is characterized in comprising the layer comprising a water-insoluble magnesium compound.

公开(公告)号：US20210198309A1

公开(公告)日：2021-07-01

申请号：US17268637

申请日：2019-08-23

Applicant: KANEKA CORPORATION

Inventor： Fuminori KONOIKE ,  Kazunobu MINAKUCHI

IPC: C07K1/34 ,  C07K1/02

Abstract: The objective of the present invention is to provide a method capable of efficiently removing an impurity from an aqueous solution or a suspension comprising an antibody or an antibody-like molecule and the impurity. The method for purifying an antibody or an antibody-like molecule according to the present invention is characterized in treating an aqueous solution or a suspension comprising the antibody or the antibody-like molecule and an impurity with a water-insoluble inorganic compound, wherein the water-insoluble inorganic compound comprises one or more elements selected from magnesium, calcium and aluminum.