公开(公告)号：US09815935B2

公开(公告)日：2017-11-14

申请号：US14783892

申请日：2014-04-14

Applicant: KENT STATE UNIVERSITY

Inventor： Elda Hegmann ,  Torsten Hegmann ,  Anshul Sharma ,  Abdollah Neshat

IPC: A61L27/56 ,  C08G63/91 ,  A61L27/58 ,  C08G63/08 ,  C09K19/38 ,  A61L27/18 ,  A61L31/06

CPC classification number: C08G63/916 ,  A61L27/18 ,  A61L27/56 ,  A61L27/58 ,  A61L31/06 ,  C08G63/08 ,  C09K19/3866 ,  C08L67/04

Abstract: Controlled biodegradable smart responsive scaffold (SRS) materials enhance attachment and viability of cells, i.e. actively guiding their expansion, proliferation and in some cases differentiation, while increasing their biomechanical functionality is an important key issue for tissue regeneration. Chemically build-in functionality in these biodegradable SRS materials is achieved by varying structural functionalization with biocompatible liquid crystal motifs and general polymer composition allowing for regulation and alteration of tensile strength, surface ordering, bioadhesion and biodegradability, bulk liquid crystal phase behavior, porosity, and cell response to external stimuli. Liquid crystal modification of such polymeric scaffolds is an ideal tool to induce macroscopic ordering events through external stimuli. None of these approaches have been demonstrated in prior art, and the use of biocompatible scaffolds that respond to a variety of external stimuli resulting in a macroscopic ordering event is a novel aspect of the present invention.

公开(公告)号：US20160339145A1

公开(公告)日：2016-11-24

申请号：US15106468

申请日：2014-12-19

Applicant: KENT STATE UNIVERSITY

Inventor： Elda Hegmann ,  Torsten Hegmann ,  Yunxiang Gao

IPC: A61L27/18 ,  A61L27/56 ,  C08J9/26 ,  C08G63/682 ,  C09K19/38 ,  C12N5/00 ,  A61L27/58

CPC classification number: A61L27/18 ,  A61L27/3834 ,  A61L27/54 ,  A61L27/56 ,  A61L27/58 ,  A61L2300/63 ,  A61L2400/00 ,  A61L2400/08 ,  C08G63/6822 ,  C08J9/26 ,  C08J2201/0442 ,  C08J2207/10 ,  C08J2367/04 ,  C09K19/3833 ,  C09K19/3838 ,  C09K2219/00 ,  C12N5/0062 ,  C12N2533/30 ,  C12N2537/10 ,  C08L67/04 ,  C08L101/12

Abstract: A polymeric scaffold contains pendant liquid crystal side chains and has fully interconnected pores. Such a polymeric scaffold will preferably be 3D in nature and elastomeric, biocompatible and biodegradable. Such 3D liquid crystal elastomer (LCE) scaffolds can be used for various biomedical applications, including cell culture applications. A method for the production of such a polymeric scaffold containing liquid crystals and having interconnected pores is also disclosed that uses a metal foam sacrificial template as a scaffold to produce the polymeric smart response scaffold of the present invention. Consistent and controlled pore sizes result from etching the sacrificial metal foam template away from the polymeric scaffold, permitting the incorporation of growth factors, when needed, for enhancing cell viability and proliferation.

Abstract translation: 聚合物支架包含侧链液晶侧链并且具有完全互连的孔。 这种聚合物支架本质上优选为3D，弹性体，生物相容性和生物降解性。 这种3D液晶弹性体（LCE）支架可用于各种生物医学应用，包括细胞培养应用。 还公开了一种用于生产含有液晶并具有互连孔的聚合物支架的方法，其使用金属泡沫牺牲模板作为支架来生产本发明的聚合智能反应支架。 通过将牺牲金属泡沫模板蚀刻离开聚合物支架，导致一致和受控的孔径，允许在需要时引入生长因子以增强细胞活力和增殖。

公开(公告)号：US10568986B2

公开(公告)日：2020-02-25

申请号：US15106468

申请日：2014-12-19

Applicant: Kent State University

Inventor： Elda Hegmann ,  Torsten Hegmann ,  Yunxiang Gao

IPC: A61L27/56 ,  C09K19/38 ,  A61L27/18 ,  C08G63/682 ,  A61L27/38 ,  A61L27/54 ,  A61L27/58 ,  C08J9/26 ,  C12N5/00

Abstract: A polymeric scaffold contains pendant liquid crystal side chains and has fully interconnected pores. Such a polymeric scaffold will preferably be 3D in nature and elastomeric, biocompatible and biodegradable. Such 3D liquid crystal elastomer (LCE) scaffolds can be used for various biomedical applications, including cell culture applications. A method for the production of such a polymeric scaffold containing liquid crystals and having interconnected pores is also disclosed that uses a metal foam sacrificial template as a scaffold to produce the polymeric smart response scaffold of the present invention. Consistent and controlled pore sizes result from etching the sacrificial metal foam template away from the polymeric scaffold, permitting the incorporation of growth factors, when needed, for enhancing cell viability and proliferation.

公开(公告)号：US10563012B2

公开(公告)日：2020-02-18

申请号：US15805510

申请日：2017-11-07

Applicant: KENT STATE UNIVERSITY

Inventor： Elda Hegmann ,  Torsten Hegmann ,  Anshul Sharma ,  Abdollah Neshat

IPC: A61L27/56 ,  C08G63/91 ,  A61L27/58 ,  A61L27/18 ,  C08G63/08 ,  C09K19/38 ,  A61L31/06

Abstract: Controlled biodegradable smart responsive scaffold (SRS) materials enhance attachment and viability of cells, i.e. actively guiding their expansion, proliferation and in some cases differentiation, while increasing their biomechanical functionality is an important key issue for tissue regeneration. Chemically build-in functionality in these biodegradable SRS materials is achieved by varying structural functionalization with biocompatible liquid crystal motifs and general polymer composition allowing for regulation and alteration of tensile strength, surface ordering, bioadhesion and biodegradability, bulk liquid crystal phase behavior, porosity, and cell response to external stimuli. Liquid crystal modification of such polymeric scaffolds is an ideal tool to induce macroscopic ordering events through external stimuli. None of these approaches have been demonstrated in prior art, and the use of biocompatible scaffolds that respond to a variety of external stimuli resulting in a macroscopic ordering event is a novel aspect of the present invention.

公开(公告)号：US10709815B2

公开(公告)日：2020-07-14

申请号：US15800446

申请日：2017-11-01

Applicant: KENT STATE UNIVERSITY

Inventor： Elda Hegmann ,  Marianne E. Prévôt ,  Torsten Hegmann

IPC: A61L27/56 ,  C08G18/28 ,  A61L27/58 ,  A61K9/70 ,  A61L27/16 ,  A61L27/38 ,  A61L27/50 ,  A61L27/14 ,  A61L27/60 ,  C08J9/26 ,  C08G18/48 ,  C08G18/42

Abstract: Star block copolymers having 3 to 8 arms are formed as a 3D foam scaffold having tailor-made pore sizes that mimic an actual cell size of a specific animal and/or human tissue and/or organs. The pore sizes are made within the elastomeric foams via a salt leaching process wherein a salt of a specific particle size is blended within the star block copolymers and crosslinked as by polyisocyanate compounds. Water or other suitable solvent are utilized to dissolve and leach out the salt leaving an open pore system. Animal and/or human cells are then injected into the 3D elastomeric foam scaffold that contains pendant liquid crystals on the star block copolymer whereby with the aid of nutrients, cells are formed within the pore system that are viable for at least three months. The size of the pore is predetermined to produce a desired cultured cell having a desired size. The tissue and/or cells within the elastomeric scaffold can be applied to animal and/or human tissue and/or organs whereupon they grow and reconstruct the damaged, injured, diseased, etc., area and result in a healthy, repaired, and viable tissue or organ. The elastomeric liquid crystal containing foam scaffold will degrade naturally and/or also be consumed by the growing cells so that it no longer exists. In other words, a specific type of animal or human cell can be culturally produced having a predetermined average cell diameter that is substantially or essentially the same diameter of a natural cell.