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公开(公告)号：US20150079585A1
公开(公告)日：2015-03-19
申请号：US14371890
申请日：2013-01-09
IPC分类号： G01N21/64
CPC分类号： G01N21/6486 , B01J2219/00576 , B01J2219/00605 , B01J2219/00711 , B01J2219/00722 , C12Q1/6874 , C12Q2523/319 , C12Q2525/186 , C12Q2565/601 , G01N21/6428 , G01N21/6458 , G01N21/648 , G01N2021/6439 , G01N2201/06113 , G01N2201/103
摘要： The present invention relates to DNA sequencing with reagent cycling on the wiregrid. The sequencing approach suggested with which allows to use a single fluid with no washing steps. Based on strong optical confinement and of excitation light and of cleavage light, the sequencing reaction can be read-out without washing the surface. Stepwise sequencing is achieved by using nucleotides with optically cleavable blocking moietys. After read-out the built in nucleotide is deblocked by cleavage light through the same substrate. This ensures that only bound nucleotides will be unblocked.
摘要翻译： 本发明涉及在丝网上循环试剂的DNA测序。 提出的测序方法允许使用不含洗涤步骤的单一流体。 基于强的光学限制和激发光和裂解光，可以读出测序反应而不洗涤表面。 通过使用具有可光学切割阻断剂的核苷酸来实现逐步测序。 读出后，内含的核苷酸通过裂解光通过相同的底物解封。 这确保只有结合的核苷酸才能被阻止。
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公开(公告)号：US10281453B2
公开(公告)日：2019-05-07
申请号：US14345261
申请日：2012-09-25
IPC分类号： G01N33/487
摘要： The invention relates to an apparatus (100) and a method for the processing of single molecules, particularly for the sensing or sequencing of single-stranded DNA. A bottom layer (110) and an electrically conductive top layer (120) with a first and a second slit (111,121), respectively, are disposed on top of each other such that an aperture (A) is formed by the slits. The slits (111,121) are preferably perpendicular to each other. An electrical circuit (140) may be connected to the top layer (120), allowing to sense single molecules that pass through the aperture (A).
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公开(公告)号：US10564104B2
公开(公告)日：2020-02-18
申请号：US15806473
申请日：2017-11-08
IPC分类号： G01N21/64 , C12Q1/6874
摘要： The present invention relates to DNA sequencing with reagent cycling on the wiregrid. The sequencing approach suggested with which allows to use a single fluid with no washing steps. Based on strong optical confinement and of excitation light and of cleavage light, the sequencing reaction can be read-out without washing the surface. Stepwise sequencing is achieved by using nucleotides with optically cleavable blocking moieties. After read-out the built in nucleotide is deblocked by cleavage light through the same substrate. This ensures that only bound nucleotides will be unblocked.
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公开(公告)号：US10393101B2
公开(公告)日：2019-08-27
申请号：US14911480
申请日：2014-07-29
发明人： Johannes Theodorus Wilhelmus Maria Van Eemeren , Irene Johanna Monica Dobbelaer-Bosboom , Pieter Jan Van Der Zaag
摘要： A microfluidic device (100) comprising at least one passive valve (PV, 120, 130), the passive valve being constituted by a sequence of a first carrier layer (CL 1) with an aperture providing a passage (P 1) for fluid flow, a first binding layer (BLI) with a first opening, a flexible layer (FL) with a through-hole (TH), a second binding layer (BL2) with a second opening, and a second carrier layer (CL2). Moreover, the flexible layer (FL) can move within a valve chamber (VC) constituted by the openings. Depending on the pressure difference across the passive valve (PV), the flexible layer (FL) can bend towards the first carrier layer (CLI) and close the aperture, while it opens the passage (P1) when bending in the opposite direction. Two of such properly oriented passive valves together with an intermediate active valve (AV) can constitute a one-stroke pump. A method for manufacturing such a fluidic device is also disclosed.
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公开(公告)号：US09823196B2
公开(公告)日：2017-11-21
申请号：US14371890
申请日：2013-01-09
CPC分类号： G01N21/6486 , B01J2219/00576 , B01J2219/00605 , B01J2219/00711 , B01J2219/00722 , C12Q1/6874 , C12Q2523/319 , C12Q2525/186 , C12Q2565/601 , G01N21/6428 , G01N21/6458 , G01N21/648 , G01N2021/6439 , G01N2201/06113 , G01N2201/103
摘要： The present invention relates to DNA sequencing with reagent cycling on the wiregrid. The sequencing approach suggested with which allows to use a single fluid with no washing steps. Based on strong optical confinement and of excitation light and of cleavage light, the sequencing reaction can be read-out without washing the surface. Stepwise sequencing is achieved by using nucleotides with optically cleavable blocking moietys. After read-out the built in nucleotide is deblocked by cleavage light through the same substrate. This ensures that only bound nucleotides will be unblocked.
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公开(公告)号：US12029500B2
公开(公告)日：2024-07-09
申请号：US17053789
申请日：2019-05-05
发明人： Pieter Jan Van Der Zaag , Christiaan Varekamp , Bernardus Hendrikus Wilhelmus Hendriks , John Pedersen
CPC分类号： A61B34/20 , A61B34/30 , A61B90/37 , A61B2034/2051 , A61B2090/374 , A61B2090/3762 , A61B2090/378
摘要： The invention refers to a system (100) for affecting a subject and a method for operating the system comprising a medical device like a biopsy gun (130) set in a first state in which it is not able to affect the subject based on a trigger signal or in a second state in which it is able to affect the subject based on the trigger signal, wherein the state is set based on a provided location of the medical device, i.e., for instance, is set based on the distance of the medical device to a target region. The location of the medical device is provided by a location providing unit (140) comprising, for instance, an imaging unit like an ultrasound system (142). Thus, unintended, possible dangerous injuries of the patient are prevented and the safety of the patient during the interventional procedure is increased.
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公开(公告)号：US11160541B2
公开(公告)日：2021-11-02
申请号：US16300327
申请日：2017-05-09
摘要： The invention relates to a biopsy container, a biopsy device, an imaging system, and a method for processing images of a biopsy container. The biopsy container comprises a container orientation mark and an alignment mark. The container orientation mark is configured for a cooperation with a corresponding shaft orientation mark arranged on a tube shaft of a biopsy device to indicate a specific orientation of the biopsy container relative to the tube shaft. The alignment mark is configured for a registration of images of the biopsy container.
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公开(公告)号：US10463349B2
公开(公告)日：2019-11-05
申请号：US15524323
申请日：2015-11-09
发明人： Pieter Jan Van Der Zaag , Cornelius Antonius Nicolaas Maria Van Der Vleuten , Bernardus Hendrikus Wilhelmus Hendriks , Gerhardus Wilhelmus Lucassen , Klaas Cornelis Jan Wijbrans
摘要： A biopsy device for taking a 3D biopsy may comprise an outer sleeve, a hollow main shaft, a biopsy tube and a tube shaft. The hollow main shaft may have a distal end portion with a sideward facing notch, and the main shaft may be adapted to be accommodated within the outer sleeve. The biopsy tube may be provided for receiving cut and thus isolated tissue. A proximal end of the biopsy tube may be releasably attachable to a distal end of the tube shaft so that the biopsy tube is movable together with the tube shaft within the hollow main shaft between a proximal position in which the biopsy tube is not located in the notch, and a distal position in which the biopsy tube is located in the notch.
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公开(公告)号：US10330598B2
公开(公告)日：2019-06-25
申请号：US15100346
申请日：2014-11-25
发明人： Johannes Joseph Hubertina Barbara Schleipen , Reinhold Wimberger-Friedl , Pieter Jan Van Der Zaag , Hendrik Paul Urbach , Mitradeep Sarkar
IPC分类号： G01N21/64 , G01N21/77 , C12Q1/6869
摘要： The invention relates to an optical device (110) and a corresponding detection apparatus (100) that may for example be used for monitoring the replication of nucleotide sequences at a surface. In a preferred embodiment, the optical device (110) comprises a waveguide substrate (130) with a wiregrid (140) on a bottom surface (132), wherein apertures (141) of the wiregrid are in at least one direction (x) smaller than a characteristic wavelength (λ) of input light (IL). Moreover, a diffractive structure (120) is disposed on the opposite surface (131) of the substrate (130) for coupling input light (IL) into the substrate (130) such that constructive interference occurs at the apertures (141). Thus evanescent waves can be generated with high efficiency in these apertures, allowing for example for a surface-specific excitation of fluorescence (FL) that can be sensed by a detector (160).
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公开(公告)号：US20180067052A1
公开(公告)日：2018-03-08
申请号：US15806473
申请日：2017-11-08
CPC分类号： G01N21/6486 , B01J2219/00576 , B01J2219/00605 , B01J2219/00711 , B01J2219/00722 , C12Q1/6874 , C12Q2523/319 , C12Q2525/186 , C12Q2565/601 , G01N21/6428 , G01N21/6458 , G01N21/648 , G01N2021/6439 , G01N2201/06113 , G01N2201/103
摘要： The present invention relates to DNA sequencing with reagent cycling on the wiregrid. The sequencing approach suggested with which allows to use a single fluid with no washing steps. Based on strong optical confinement and of excitation light and of cleavage light, the sequencing reaction can be read-out without washing the surface. Stepwise sequencing is achieved by using nucleotides with optically cleavable blocking moieties. After read-out the built in nucleotide is deblocked by cleavage light through the same substrate. This ensures that only bound nucleotides will be unblocked.
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