公开(公告)号：US20190112575A1

公开(公告)日：2019-04-18

申请号：US16095092

申请日：2017-04-24

申请人： KYOTO UNIVERSITY ,  SUMITOMO DAINIPPON PHARMA CO., LTD.

发明人： Jun TAKAHASHI ,  Daisuke DOI ,  Kenji YOSHIDA ,  Atsushi KUWAHARA ,  Masayo TAKAHASHI

IPC分类号： C12N5/0793 ,  C12N5/074

摘要： A cell population comprising Corin- and/or Lrtm1-positive cells was produced by the following steps (1) and (2), from which Corin positive and/or Lrtm1 positive cells are collected using a substance that binds to Corin and/or a substance that binds to Lrtm1, and dopaminergic neuron progenitor cells are produced by performing suspension culture of the Corin positive and/or Lrtm1 positive cells in a culture solution containing one or more nutritional factors: (1) a step of performing adhesion culture of pluripotent stem cells in a medium for maintaining undifferentiated state containing a Sonic hedgehog (SHH) signal stimulant, and an undifferentiated state-maintaining factor in the absence of feeder cells but in the presence of an extracellular matrix, and (2) a step of culturing the cell population obtained in the step (1) in a culture solution containing one or more differentiation-inducing factors.

公开(公告)号：US20240117304A1

公开(公告)日：2024-04-11

申请号：US18539589

申请日：2023-12-14

申请人： KYOTO UNIVERSITY ,  SUMITOMO PHARMA CO., LTD.

发明人： Jun TAKAHASHI ,  Daisuke DOI ,  Kenji YOSHIDA ,  Atsushi KUWAHARA ,  Masayo TAKAHASHI

IPC分类号： C12N5/0793 ,  A61K35/30 ,  C12N5/074

CPC分类号： C12N5/0619 ,  A61K35/30 ,  C12N5/0696 ,  C12N2500/38 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/13 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/41 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/45 ,  C12N2533/90

摘要： A cell population comprising Corin- and/or Lrtm1-positive cells was produced by the following steps (1) and (2), from which Corin positive and/or Lrtm1 positive cells are collected using a substance that binds to Corin and/or a substance that binds to Lrtm1, and dopaminergie neuron progenitor cells are produced by performing suspension culture of the Corin positive and/or Lrtm1 positive cells in a culture solution containing one or more nutritional factors:



(1) a step of performing adhesion culture of pluripotent stem cells in a medium for maintaining undifferentiated state containing a Sonic hedgehog (SHH) signal stimulant, and an undifferentiated state-maintaining factor in the absence of feeder cells but in the presence of an extracellular matrix, and
(2) a step of culturing the cell population obtained in the step (1) in a culture solution containing one or more differentiation-inducing factors.