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公开(公告)号：US5702927A
公开(公告)日：1997-12-30
申请号：US484477
申请日：1995-06-07
CPC分类号： C08B37/0033 , C12P19/06 , Y10S435/822 , Y10S435/91
摘要： A xanthan gum having an improved transmittance is disclosed. Further, the transmittance of the xanthan gum is at least 75% in an 1% by weight aqueous solution. The xanthan gum is prepared by subjecting a strain ATCC 55429 or ATCC 55298 to submerged fermentation conditions to produce a broth containing the gum and debris. The broth is then subjected to heating at a temperature from 45.degree. to 70 .degree. C. for a period of time of at least one half to two hours and at a pH of 9 to 12.5. Two enzyme treatments are performed using an alkaline protease and lysozyme of which the order of their application is irrelevant to the recovery of the xanthan gum. The enzymes are applied at specific temperatures and time periods as well as pH conditions such as a protease is applied at a temperature of 40.degree. to 65 .degree. C. for a period of from 20 minutes to five hours at a pH range of 6 to 10; and the lysozyme is applied at a temperature of 25.degree. to 60 .degree. C. for a period of 20 minutes to five hours at a pH range of 5.5 to 8.0. Both of the enzyme treatments are applied after the broth has been heated in step b) and before the recovery of the xanthan gum.
摘要翻译： 公开了具有改善的透光率的黄原胶。 此外，在1重量％水溶液中，黄原胶的透光率为至少75％。 黄原胶通过使菌株ATCC 55429或ATCC 55298经受浸没发酵条件制备含有牙龈和碎屑的肉汤来制备。 然后在45℃至70℃的温度下将肉汤加热至少一半至两小时，pH为9至12.5。 使用碱性蛋白酶和溶菌酶进行两种酶处理，其应用顺序与黄原胶的回收无关。 酶在特定的温度和时间段以及pH条件例如蛋白酶在40至65℃的温度下施用，时间为20分钟至5小时，pH范围为6至10 ; 将溶菌酶在25〜60℃的温度下，在5.5〜8.0的pH范围内进行20分钟〜5小时。 在肉汤在步骤b）中加热并且在黄原胶回收之前，都应用两种酶处理。
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公开(公告)号：US5595892A
公开(公告)日：1997-01-21
申请号：US345076
申请日：1994-11-28
CPC分类号： C08B37/0033 , C12P19/06 , Y10S435/822 , Y10S435/91
摘要： The process includes heat-treating a xanthan gum fermented broth, and consecutively treating the broth first with alkaline protease and then with lysozyme or in reverse order, and thereafter recovering xanthan gum from the treated broth. A clear aqueous solution of xanthan gum may be obtained Without complex procedures.
摘要翻译： 该方法包括热处理黄原胶发酵肉汤，首先用碱性蛋白酶，然后用溶菌酶或相反的顺序连续处理肉汤，然后从处理的肉汤中回收黄原胶。 无需复杂的操作即可获得透明的黄原胶水溶液。
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3.发明授权Process for preparing and selecting hyperproducing microorganism strains used for the process for producing streptovaricin C 失效制备和选择用于生产链脲霉素C的方法的超生产微生物菌株的方法
公开(公告)号：US5210033A
公开(公告)日：1993-05-11
申请号：US872519
申请日：1992-04-23
IPC分类号： C12P17/18
CPC分类号： C12R1/465 , C12P17/18 , Y10S435/803 , Y10S435/886
摘要： Disclosed is a method for selecting a mutant strain belonging to the genus Streptomyces which is a hyper-producer of Streptovaricin C superior to those know heretofore. This is accomplished by first subjecting a natural strain of Streptomyces spectabilis to conditions so as to isolate organisms which are streptovaricin resistant. The streptovaricin resistant organisms thus isolated are then subjected to mutagenesis and then cultured. The colonies which are asporogenous are individually cultured in fermentation batches such that the strains take the form of pellets of varying sizes and colors. From the batch having the most heterogeneous mixture of pellets, the smallest pellet or the pellet(s) having the deepest color (usually deep red or crimson) is isolated. We have discovered that the strain of this pellet has a high likelihood of being a hyperproducer of streptovaricin. The strain of this pellet may then be subjected to fermentation conditions to produce streptovaricin. The nutrient broth containing fumaric acid or water-soluble salts thereof, and adsorbent polymer beads and the streptovaricin produced are recovered in the usual manner. In another embodiment, the fermentation may be carried out in a nutrient broth wherein fumaric acid or its water-soluble salts are deliberately excluded to achieve even higher productivity.
摘要翻译： 公开了一种选择属于链霉菌属（Streptomyces）属的突变菌株的方法，其是链节链球菌C的超级生产者，其比迄今所知道的更高。 这是通过首先对天然链霉菌链霉菌进行条件来分离的，以分离链霉素抗性的生物体。 然后将如此分离的链脲霉素抗性生物体进行诱变，然后进行培养。 在发酵批次中单独培养异源菌落，使得菌株呈不同大小和颜色的颗粒的形式。 从具有最不均匀的颗粒混合物的批料中，分离出具有最深颜色（通常深红色或深红色）的最小颗粒或颗粒。 我们已经发现，这种颗粒的菌株很可能成为链球菌素的超生产菌。 然后可以将该颗粒的菌株进行发酵条件以产生链脲霉素。 以通常的方式回收含有富马酸或其水溶性盐的营养液，以及生成的吸附性聚合物珠粒和链球菌素。 在另一个实施方案中，发酵可以在营养肉汤中进行，其中富马酸或其水溶性盐被有意排除以达到更高的生产率。
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4.发明授权
公开(公告)号：US5266484A
公开(公告)日：1993-11-30
申请号：US872518
申请日：1992-04-23
CPC分类号： C12R1/465 , C12P17/18 , Y10S435/803 , Y10S435/886
摘要： Disclosed is a method for selecting a mutant strain belonging to the genus Streptomyces which is a hyper-producer of Streptovaricin C superior to those know heretofore. This is accomplished by first subjecting a natural strain of Streptomyces spectabilis to conditions so as to isolate organisms which are streptovaricin resistant. The streptovaricin resistant organisms thus isolated are then subjected to mutagenesis and then cultured. The colonies which are asporogenous are individually cultured in fermentation batches such that the strains take the form of pellets of varying sizes and colors. From the batch having the most heterogeneous mixture of pellets, the smallest pellet or the pellet(s) having the deepest color (usually deep red or crimson) is isolated. We have discovered that the strain of this pellet has a high likelihood of being a hyperproducer of streptovaricin. The strain of this pellet may then be subjected to fermentation conditions to produce streptovaricin. The nutrient broth containing fumaric acid or water-soluble salts thereof, and adsorbent polymer beads and the streptovaricin produced are recovered in the usual manner. In another embodiment, the fermentation may be carried out in a nutrient broth wherein fumaric acid or its water-soluble salts are deliberately excluded to achieve even higher productivity.
摘要翻译： 公开了一种选择属于链霉菌属（Streptomyces）属的突变菌株的方法，其是链节链球菌C的超级生产者，其比迄今所知道的更高。 这是通过首先对天然链霉菌链霉菌进行条件来分离的，以分离链霉素抗性的生物体。 然后将如此分离的链脲霉素抗性生物体进行诱变，然后进行培养。 在发酵批次中单独培养异源菌落，使得菌株呈不同大小和颜色的颗粒的形式。 从具有最不均匀的颗粒混合物的批料中，分离出具有最深颜色（通常深红色或深红色）的最小颗粒或颗粒。 我们已经发现，这种颗粒的菌株很可能成为链球菌素的超生产菌。 然后可以将该颗粒的菌株进行发酵条件以产生链脲霉素。 以通常的方式回收含有富马酸或其水溶性盐的营养液，以及生成的吸附性聚合物珠粒和链球菌素。 在另一个实施方案中，发酵可以在营养肉汤中进行，其中富马酸或其水溶性盐被有意排除以达到更高的生产率。
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公开(公告)号：US5208153A
公开(公告)日：1993-05-04
申请号：US776989
申请日：1991-10-16
CPC分类号： C12R1/465 , C12P17/18 , Y10S435/886
摘要： Disclosed is a method for selecting a mutant strain belonging to the genus Streptomyces which is a hyper-producer of Streptovaricin C superior to those know heretofore. This is accomplished by first subjecting a natural strain of Streptomyces spectabilis to conditions so as to isolate organisms which are streptovaricin resistant. The streptovaricin resistant organisms thus isolated are then subjected to mutagenesis and then cultured. The colonies which are asporogenous are individually cultured in fermentation batches such that the strains take the form of pellets of varying sizes and colors. From the batch having the most heterogeneous mixture of pellets, the smallest pellet or the pellet(s) having the deepest color (usually deep red or crimson) is isolated. We have discovered that the strain of this pellet has a high likelihood of being a hyperproducer of streptovaricin. The strain of this pellet may then be subjected to fermentation conditions to produce streptovaricin. The nutrient broth containing fumaric acid or water-soluble salts thereof, and adsorbent polymer beads and the streptovaricin produced are recovered in the usual manner. In another embodiment, the fermentation may be carried out in a nutrient broth wherein fumaric acid or its water-soluble salts are deliberately excluded to achieve even higher productivity.
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公开(公告)号：US09237763B2
公开(公告)日：2016-01-19
申请号：US13213663
申请日：2011-08-19
IPC分类号： A23K3/00 , A23F3/16 , A23K1/00 , A23B7/10 , A23F3/00 , A23L1/22 , A23L1/20 , A23L1/182 , A01N63/00 , A61K47/00 , C12P19/04 , C12N1/20 , A23L1/30 , A23K1/16 , A23L1/308 , A61K35/742
CPC分类号： A23L1/3014 , A23K10/18 , A23K20/163 , A23L33/135 , A23L33/21 , A23V2002/00 , A61K35/742
摘要： The present invention relates to a synbiotic product composition comprising a blend or mixture of a prebiotic carbohydrate and a probiotic spore-forming Bacillus bacteria. Examples of prebiotic carbohydrates useful in synbiotic product include arabinoxylan, arabinoxylan oligosaccharides, xylose, soluble fiber dextrin, soluble corn fiber, and polydextrose. The present invention also relates to human foodstuffs and animal feed comprising such synbiotic products and methods of increasing the titer of spore-forming bacteria in the intestinal tracts of mammals by administration of symbiotic products.
摘要翻译： 本发明涉及包含益生元碳水化合物和产生益生菌孢子芽孢杆菌的共混物或混合物的合生素产品组合物。 可用于合生产物的益生元碳水化合物的实例包括阿拉伯木聚糖，阿拉伯木聚糖寡糖，木糖，可溶性纤维糊精，可溶性玉米纤维和聚右旋糖。 本发明还涉及包含这些合生素产物的人类食品和动物饲料以及通过施用共生产物来增加哺乳动物肠道中的孢子形成细菌滴度的方法。
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公开(公告)号：US20120052152A1
公开(公告)日：2012-03-01
申请号：US13213663
申请日：2011-08-19
CPC分类号： A23L1/3014 , A23K10/18 , A23K20/163 , A23L33/135 , A23L33/21 , A23V2002/00 , A61K35/742
摘要： The present invention relates to a synbiotic product composition comprising a blend or mixture of a prebiotic carbohydrate and a probiotic spore-forming Bacillus bacteria. Examples of prebiotic carbohydrates useful in synbiotic product include arabinoxylan, arabinoxylan oligosaccharides, xylose, soluble fiber dextrin, soluble corn fiber, and polydextrose. The present invention also relates to human foodstuffs and animal feed comprising such synbiotic products and methods of increasing the titer of spore-forming bacteria in the intestinal tracts of mammals by administration of symbiotic products.
摘要翻译： 本发明涉及包含益生元碳水化合物和产生益生菌孢子芽孢杆菌的共混物或混合物的合生素产品组合物。 可用于合生产物的益生元碳水化合物的实例包括阿拉伯木聚糖，阿拉伯木聚糖寡糖，木糖，可溶性纤维糊精，可溶性玉米纤维和聚右旋糖。 本发明还涉及包含这些合生素产物的人类食品和动物饲料以及通过施用共生产物来增加哺乳动物肠道中的孢子形成细菌滴度的方法。
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8.发明授权Biologically pure culture of bacillus ATCC 55294 that produces an endoglycanase 失效产生凝集素的杆菌ATCC 55294的生物学纯培养物
公开(公告)号：US5401659A
公开(公告)日：1995-03-28
申请号：US251600
申请日：1994-05-31
CPC分类号： C12R1/07 , C04B24/38 , C12N9/2402 , C12N9/2437 , C12P19/04 , Y10S435/832
摘要： An isolated endogly canase having activity against polysaccharides having the subunit backbone structure: ##STR1## wherein Glc is glucose, GlcA is glucuronic acid, Rha is rhamnose, Man is mannose, X may be Rha or Man, and wherein the reducing end of the polymer is toward the X residue of the backbone is disclosed. A method for producing the compound by culturing Bacillus ATCC 55294, the method for selecting Bacillus ATCC 55294 and method for using the endoglycanase are also disclosed.
摘要翻译： 其中Glc是葡萄糖，GlcA是葡萄糖醛酸，Rha是鼠李糖，Man是甘露糖，X可以是Rha或Man，并且其中 公开了聚合物的还原端朝向骨架的X残基。 还公开了通过培养芽孢杆菌ATCC 55294，选择芽孢杆菌ATCC 55294的方法和使用内聚聚糖酶的方法来生产化合物的方法。
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公开(公告)号：US5190866A
公开(公告)日：1993-03-02
申请号：US826371
申请日：1992-01-27
CPC分类号： C12P17/18 , C12N1/02 , C12P1/00 , Y10S435/803 , Y10S435/886
摘要： A method for separation and recovery of polymeric beads from an antibiotic fermentation broth comprising suspending a mixture of the beads and any inherent mold from the fermentation in an aqueous solution having a specific gravity which is effective to cause the beads to form a discrete layer at or on the surface of the solution, separate and apart from the mold. The separate layer of the beads may then be easily removed from the liquid by a conventional physical methods.
摘要翻译： 一种用于从抗生素发酵液中分离和回收聚合物珠的方法，其包括将珠粒和任何固有的霉菌的混合物从发酵中悬浮在具有比重的水溶液中，该水溶液有效地使珠粒在或 在溶液的表面上，与模具分离并分开。 然后可以通过常规的物理方法将珠的单独层容易地从液体中除去。
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10.发明授权公开(公告)号：US08741621B2
公开(公告)日：2014-06-03
申请号：US12571743
申请日：2009-10-01
CPC分类号： C07K14/43
摘要： Recombinant P. pastoris producing natural sweet proteins and methods for engineering these recombinant yeast are described. Methods for enhancing foreign protein production in yeast fermentation and improved methods for purification of foreign proteins produced in yeast fermentation are presented.
摘要翻译： 描述了产生天然甜蛋白的重组巴斯德毕赤酵母和用于工程化这些重组酵母的方法。 提出了增加酵母发酵中外源蛋白质生产的方法，并提出了酵母发酵产生的外源蛋白质纯化方法。
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