公开(公告)号：US20060247193A1

公开(公告)日：2006-11-02

申请号：US10544761

申请日：2004-02-10

申请人： Kazunari Taira ,  Hiroaki Kawasaki

发明人： Kazunari Taira ,  Hiroaki Kawasaki

IPC分类号： A61K48/00 ,  C12N15/09

CPC分类号： C12N15/113 ,  C12N2310/14 ,  C12N2310/141 ,  C12N2310/531

摘要： The present invention provides products and methods for modulating expression of a target gene in a cell. One such method includes introducing into the cell a polynucleotide that forms a duplex region with an mRNA transcribed from said target gene, where the duplex region comprises a mammalian miRNA target region. Another such method includes introducing into the cell an siRNA that forms a duplex region with an miRNA, or precursor thereof, where an mRNA transcribed from the target gene comprises a miRNA target region. In certain preferred embodiments, the methods further include measuring expression of the target gene. The methods are particularly useful for modulating ontogenesis, function, differentiation and/or viability of a mammalian cell. As such, the invention also provides methods for controlling ontogenesis of mammal, function of mammalian cell, differentiation of mammalian cell or viability of mammalian cell in the post-transcriptional phase by introducing into the cell a miRNA or a siRNA silencing precursor to the miRNA. The invention additionally provides polynucleotides, including vectors, useful in the method of the instant invention. The provided polynucleotides include a plasmid vector comprising a promoter and a polynucleotide sequence expressing miRNA or precursor to the miRNA. Also included is a plasmid vector comprising a promoter and a nucleotide sequence expressing siRNA silencing precursor to miRNA. In certain preferred embodiments, the mRNA is capable of forming a duplex region with an mRNA transcribed from a mammalian target gene.

摘要翻译： 本发明提供调节细胞中靶基因表达的产物和方法。 一种这样的方法包括向细胞中引入与从所述靶基因转录的mRNA形成双链体区的多核苷酸，其中双链区包含哺乳动物miRNA靶区。 另一种这样的方法包括向细胞中引入与miRNA或其前体形成双链体区的siRNA，其中从靶基因转录的mRNA包含miRNA靶区。 在某些优选实施方案中，所述方法还包括测量靶基因的表达。 该方法对于调节哺乳动物细胞的发生，功能，分化和/或活力特别有用。 因此，本发明还提供了通过将miRNA或siRNA沉默前体引入细胞来控制哺乳动物细胞的发生，哺乳动物细胞的功能，哺乳动物细胞的分化或哺乳动物细胞在转录后阶段的存活力的方法。 本发明另外提供了可用于本发明方法的多核苷酸，包括载体。 所提供的多核苷酸包括质粒载体，其包含启动子和表达miRNA或miRNA前体的多核苷酸序列。 还包括质粒载体，其包含启动子和表达miRNA沉默前体的核苷酸序列。 在某些优选的实施方案中，mRNA能够与从哺乳动物靶基因转录的mRNA形成双链体区域。

公开(公告)号：US20060045873A1

公开(公告)日：2006-03-02

申请号：US11209044

申请日：2005-08-23

申请人： Kazunari Taira ,  Hiroaki Kawasaki

发明人： Kazunari Taira ,  Hiroaki Kawasaki

IPC分类号： C12N15/87 ,  A61K48/00

CPC分类号： C12N15/85 ,  A01K2217/05 ,  A01K2267/0381 ,  A61K31/7088 ,  C12N15/111 ,  C12N15/8509 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/3519 ,  C12N2310/53 ,  C12N2330/30 ,  C12N2830/008 ,  C12N2830/42 ,  C12N2830/46

摘要： The present inventors found that in human cells, synthetic small interfering RNAs (siRNAs) targeted to a region containing the CpG islands on gene promoters will specifically induce the methylation of CpG sequences within the target region or adjacent regions. Methylation of the promoters results in suppression at a transcriptional level of expression of genes downstream of the promoters. The present invention provides methods for inducing DNA methylation using the siRNAs, and methods for suppressing gene expression based on siRNA-directed methylation of promoters. The present invention also provides DNA methylation-inducing agents or gene expression-suppressing agents that comprise siRNAs or expression vectors for the siRNAs or dsRNAs.

摘要翻译： 本发明人发现，在人类细胞中，靶向包含基因启动子上的CpG岛的区域的合成小干扰RNA（siRNA）将特异性诱导靶区域或相邻区域内的CpG序列的甲基化。 启动子的甲基化导致抑制启动子下游基因表达的转录水平。 本发明提供使用siRNA诱导DNA甲基化的方法，以及基于siRNA引导的启动子甲基化抑制基因表达的方法。 本发明还提供了包含用于siRNA或dsRNA的siRNA或表达载体的DNA甲基化诱导剂或基因表达抑制剂。

公开(公告)号：US20060088837A1

公开(公告)日：2006-04-27

申请号：US10512386

申请日：2003-04-25

申请人： Kazunari Taira ,  Hiroaki Kawasaki

发明人： Kazunari Taira ,  Hiroaki Kawasaki

IPC分类号： C12Q1/68 ,  A01K67/027 ,  C07H21/02 ,  A61K48/00

CPC分类号： A01K67/0275 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2217/20 ,  A01K2227/101 ,  A01K2227/103 ,  A01K2227/105 ,  A01K2227/106 ,  A01K2227/107 ,  A01K2227/108 ,  A61K31/7088 ,  A61K48/00 ,  A61K2121/00 ,  C12N15/111 ,  C12N15/85 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/3519 ,  C12N2310/53 ,  C12N2330/30 ,  C12N2830/00 ,  C12N2830/003 ,  C12N2830/85

摘要： The present inventors discovered that an RNA molecule can be efficiently transferred into cytoplasm and exert RNAi effects, by producing a stem loop RNA molecule from the DNA that encodes this RNA molecule, with the use of a tRNA promoter. Also, the RNAi effects can be exerted effectively, by introducing a cytoplasm translocation signal sequence into the DNA that encodes a stem loop RNA molecule. Moreover, the RNAi effects can be exerted effectively by transferring a transcriptional product into the cytoplasm, using a pol II-type promoter. In this case, cytotoxicity can be reduced by co-expressing a Dicer gene. Furthermore, an effective dsRNA can be constructed by treating a dsRNA or a stem loop RNA molecule with Dicer protein. Knockout cells lacking a gene of interest can be conveniently constructed, using these stem-loop RNA molecule expression systems.

摘要翻译： 本发明人通过使用tRNA启动子，通过从编码该RNA分子的DNA产生茎环RNA分子，可以有效地将RNA分子转移到细胞质中并发挥RNAi作用。 此外，通过将细胞质易位信号序列引入到编码茎环RNA分子的DNA中，可以有效地发挥RNAi作用。 此外，通过使用pol II型启动子将转录产物转移到细胞质中可以有效地发挥RNAi作用。 在这种情况下，可以通过共表达Dicer基因来减少细胞毒性。 此外，通过用Dicer蛋白处理dsRNA或茎环RNA分子可以构建有效的dsRNA。 使用这些茎环RNA分子表达系统，可以方便地构建缺乏目的基因的敲除细胞。

公开(公告)号：US09293954B2

公开(公告)日：2016-03-22

申请号：US13983895

申请日：2011-12-06

申请人： Hiroaki Kawasaki ,  Yuichi Yoshikawa

发明人： Hiroaki Kawasaki ,  Yuichi Yoshikawa

IPC分类号： H02K21/12 ,  H02K1/27 ,  H02K21/16

CPC分类号： H02K1/2706 ,  H02K1/276 ,  H02K21/16

摘要： Provided are a rotor of a motor capable of attaining a high-speed rotation by appropriately dispersing a stress exerted on a flux barrier portion while reducing a leakage of magnetic flux, and a fan driving motor including the rotor. A second inner wall surface is greater than a first inner wall surface in length in a cross-section perpendicular to a rotational axis (C). A side wall surface has a flux barrier wall surface extending from each of both ends of the second inner wall surface in the cross-section perpendicular to the rotational axis (C), the flux barrier wall surface extending outward relative to the second inner wall surface in an extending direction of the second inner wall surface and more distant from an outer edge of a rotor as it goes from the second inner wall surface toward the first inner wall surface in the cross-section perpendicular to the rotational axis (C).

摘要翻译： 提供一种能够通过适当地分散施加在磁通屏障部分上的应力来实现高速旋转的电动机的转子，同时减少磁通量的泄漏，以及包括转子的风扇驱动电动机。 在垂直于旋转轴线（C）的横截面中，第二内壁表面的长度大于第一内壁表面。 侧壁表面具有从垂直于旋转轴线（C）的横截面中从第二内壁表面的两端各自延伸的通量阻挡壁表面，阻挡壁表面相对于第二内壁表面向外延伸 在第二内壁表面的延伸方向上，在与第二内壁表面垂直于旋转轴线（C）的横截面中从第二内壁表面朝向第一内壁表面更远离转子的外边缘。

公开(公告)号：US09149794B2

公开(公告)日：2015-10-06

申请号：US13266773

申请日：2009-12-03

申请人： Yoshikazu Kawai ,  Hiroaki Kawasaki ,  Naomi Kawahara

发明人： Yoshikazu Kawai ,  Hiroaki Kawasaki ,  Naomi Kawahara

IPC分类号： C07K16/00 ,  B01J20/32 ,  B01J20/289

CPC分类号： C08B37/00 ,  B01J20/289 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3229 ,  B01J20/3244 ,  B01J20/3248

摘要： The present invention relates to a method for producing a formyl group-containing porous base matrix, comprising the steps of introducing a spacer in a formyl group-containing porous particle; and then oxidizing the spacer with periodic acid and/or a periodate, to transform the part of the spacer into a formyl group; wherein the formyl group content in the porous particle after introduction of the spacer is not more than 3 μmol per 1 mL of the porous particle. Also, the present invention relates to a method for producing an adsorbent, comprising the step of immobilizing an amino group-containing ligand on the formyl group-containing porous base matrix. According to the present invention, a formyl-group containing porous base matrix and an adsorbent produced from the porous base matrix of which adsorption amount is high and which is has high strength and of which ligand is difficult to be leaked are provided.

摘要翻译： 本发明涉及一种含有甲酰基的多孔基质基质的制造方法，包括以下步骤：在含甲酰基的多孔质粒子中引入间隔物; 然后用高碘酸和/或高碘酸盐氧化间隔物，以将部分间隔物转化成甲酰基; 其中间隔物引入后的多孔质粒中的甲酰基含量相对于1mL多孔质粒不超过3μmol。 此外，本发明涉及一种吸附剂的制造方法，其特征在于，含有含氨基的配位体固定在含甲酰基的多孔质基体上的工序。 根据本发明，提供了含有甲酰基的多孔基质基质和由吸附量高，强度高，配体难以泄漏的多孔基质基质生成的吸附剂。

公开(公告)号：US08958940B2

公开(公告)日：2015-02-17

申请号：US13241347

申请日：2011-09-23

申请人： Hiroaki Kawasaki

发明人： Hiroaki Kawasaki

IPC分类号： G06F7/00 ,  G01G19/08 ,  B60C23/06

CPC分类号： G01G19/086 ,  B60C23/062

摘要： A vehicle mass estimation apparatus includes a wheel rotation speed information detection device configured to detect rotation speed information regarding the respective wheels of the vehicle; a frequency characteristic estimation device configured to calculate wheel acceleration information based on the rotation speed information of the wheels to estimate a frequency characteristic of the wheel acceleration information; and a vehicle mass estimation device configured to estimate a vehicle mass based on the estimated frequency characteristic. The frequency characteristic estimation device calculates an integrated value of frequency gain magnitudes of the respective frequency domains of front wheels and rear wheels of the vehicle, and the vehicle mass estimation device estimates the vehicle mass based on the ratio among the respective integrated values of the front wheels and rear wheels and a relation calculated in advance between the ratio and the vehicle mass.

摘要翻译： 车辆质量估计装置包括车轮转速信息检测装置，其被配置为检测关于车辆的各车轮的转速信息; 频率特性估计装置，被配置为基于所述车轮的转速信息来计算车轮加速度信息，以估计所述车轮加速度信息的频率特性; 以及车辆质量估计装置，其被配置为基于所估计的频率特性来估计车辆质量。 频率特性估计装置计算车辆的前轮和后轮的各个频域的频率增益量的积分值，车辆质量估计装置基于前方的各个积分值之间的比率来估计车辆质量 车轮和后轮以及在比率和车辆质量之间预先计算的关系。

公开(公告)号：US08299909B2

公开(公告)日：2012-10-30

申请号：US12497283

申请日：2009-07-02

申请人： Hajime Fujita ,  Hiroaki Kawasaki

发明人： Hajime Fujita ,  Hiroaki Kawasaki

IPC分类号： G08B21/00

CPC分类号： B60C23/061 ,  B60C23/062

摘要： An apparatus for detecting a decrease in a tire air pressure includes; a rotation velocity information detection means for regularly detecting tire rotation velocity information regarding the respective wheels of a vehicle; a resonance frequency estimation means for estimating a resonance frequency of the rotation velocity information based on the rotation velocity information obtained by the rotation velocity information detection means; and a determination means for determining, based on the estimated resonance frequency, a decrease in the tire air pressure. The apparatus includes a data rejecting means for rejecting data of rotation velocity information hindering the estimation of the resonance frequency.

摘要翻译： 用于检测轮胎气压降低的装置包括： 旋转速度信息检测装置，用于定期检测关于车辆的各个车轮的轮胎转速信息; 谐振频率估计装置，用于基于由所述旋转速度信息检测装置获得的旋转速度信息来估计所述旋转速度信息的共振频率; 以及确定装置，用于基于所估计的共振频率来确定轮胎空气压力的降低。 该装置包括用于拒绝阻碍谐振频率的估计的旋转速度信息的数据的数据拒绝装置。

公开(公告)号：US08159102B2

公开(公告)日：2012-04-17

申请号：US12813166

申请日：2010-06-10

申请人： Yuichi Yoshikawa ,  Hiroaki Kawasaki ,  Kenji Sugiura ,  Hiroshi Ueda ,  Hiroki Asai

发明人： Yuichi Yoshikawa ,  Hiroaki Kawasaki ,  Kenji Sugiura ,  Hiroshi Ueda ,  Hiroki Asai

IPC分类号： H02K7/00

CPC分类号： H02K11/225 ,  H02K1/146

摘要： A rotor 14 is rotatably disposed around a stator 13 that is provided with a plurality of magnetic poles 13a. The inner circumferential face of a rotor frame 14b of the rotor is provided with a magnet 15 magnetized to have alternately opposite polarities in a direction opposing the stator, and magnetized to have alternately opposite polarities in a direction opposing a substrate. The outer circumferential ends of the magnetic poles of the stator are provided with a first extended portion 13c that extends from a magnetic pole base 13d to a substrate 11 side, and a second extended portion 13b that extends from the magnetic pole base to a side opposite the substrate side. A face of the substrate opposing the rotor is provided with a FG pattern 19. When a distance between an end of the rotor frame on the substrate side and the substrate is taken as A, a distance between an end of the first extended portion on the substrate side and the substrate is taken as B, and a distance between the end of the first extended portion on the substrate side and the magnet is taken as G, B≦A≦B+G is satisfied. Accordingly, it is possible to improve the precision in detecting the rotational speed by reducing noise superimposed on the FG signal, while securing a high driving efficiency.

摘要翻译： 转子14围绕设置有多个磁极13a的定子13可旋转地设置。 转子的转子架14b的内周面设置有磁体15，该磁体被磁化成与定子相反的方向具有相反的极性，并且在与基板相对的方向上被磁化成具有相反的极性。 定子的磁极的外周端设置有从磁极基部13d延伸到基板11侧的第一延伸部13c和从磁极基部延伸到相反侧的第二延伸部13b 基板侧。 与转子相对的基板的表面设置有FG图案19.当基板侧的转子框架的端部与基板之间的距离取为A时，第一延伸部分的端部之间的距离 将基板侧和基板取为B，将基板侧的第一延伸部的端部与磁铁的距离设为G，B＆nlE;满足A＆lt; NlE; B + G。 因此，在确保高驱动效率的同时，通过降低叠加在FG信号上的噪音来提高检测转速的精度。

公开(公告)号：US08159097B2

公开(公告)日：2012-04-17

申请号：US12813258

申请日：2010-06-10

申请人： Yuichi Yoshikawa ,  Hiroaki Kawasaki ,  Kenji Sugiura ,  Hiroshi Ueda ,  Hiroki Asai

发明人： Yuichi Yoshikawa ,  Hiroaki Kawasaki ,  Kenji Sugiura ,  Hiroshi Ueda ,  Hiroki Asai

IPC分类号： H02K7/00

CPC分类号： H02K29/03 ,  H02K1/146 ,  H02K1/2786

摘要： A rotor 14 is rotatably disposed around a stator 13 that is provided with a plurality of magnetic poles 13a. The inner circumferential face of the rotor is provided with a magnet 15 on whose face opposing the stator main magnetization is performed, and on whose face opposing the substrate FG magnetization is performed. The outer circumferential ends of the magnetic poles of the stator are provided with extended portions 13b and 13c that vertically extend from the magnetic pole base 13d. A face of the substrate opposing the rotor is provided with a FG pattern 19 opposing the magnet 15. The FG pattern is disposed on an outer side in a radial direction of the outer circumferential face of the stator. When an angle formed by a straight line connecting a central position in the radial direction of the magnet and a central position in the radial direction of the FG pattern with respect to a central axis of a driving shaft is taken as θ, and an angle formed by a direction of the FG magnetization with respect to the central axis of the driving shaft is taken as A, 0 degree

摘要翻译： 转子14围绕设置有多个磁极13a的定子13可旋转地设置。 转子的内周面设置有磁铁15，在磁体15的与定子主磁化相反的方向上进行磁体15，并且在其表面与基板FG的磁化相反的方向被执行。 定子的磁极的外周端设置有从磁极基座13d垂直延伸的延伸部分13b和13c。 与转子相对的基板的表面设置有与磁体15相对的FG图案19.GV图案设置在定子的外周面的径向外侧。 当将连接磁体径向中心位置的直线和FG图案径向方向上的中心位置相对于驱动轴的中心轴线形成的角度取为角度时， 由相对于驱动轴的中心轴线的FG磁化方向形成的A为0度

公开(公告)号：US20100256416A1

公开(公告)日：2010-10-07

申请号：US12734661

申请日：2008-11-07

申请人： Hiroaki Kawasaki ,  Katsuji Maehara ,  Tadashi Moroshima

发明人： Hiroaki Kawasaki ,  Katsuji Maehara ,  Tadashi Moroshima

IPC分类号： C07C229/26

CPC分类号： C07C273/1827 ,  C07C275/16

摘要： The present invention relates to a process for producing an N-carbamoyl-tert-leucine, characterized in mixing tert-leucine with an isocyanic acid compound while a pH of the mixture is kept at not less than 8.0 and not more than 13.5, wherein an amount of the isocyanic acid compound is not less than 0.9 times by mole and not more than 1.1 times by mole relative to an amount of the tert-leucine. According to the present invention, it becomes possible to easily produce an N-carbamoyl-tert-leucine with high efficiency, while the generation of by-products such as a dipeptide-like compound and a urea compound is prevented.

摘要翻译： 本发明涉及一种N-氨基甲酰基 - 叔 - 亮氨酸的制备方法，其特征在于，将所述混合物的pH值保持在8.0以上且13.5以下的条件下，将叔亮氨酸与异氰酸化合物混合， 异氰酸化合物的量相对于叔亮氨酸的量为0.9倍摩尔以上1.1倍以下。 根据本发明，可以高效率地容易地制造N-氨基甲酰基 - 叔 - 亮氨酸，同时防止二肽类化合物和尿素化合物等副产物的产生。