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    Immunoassay element
    2.
    发明申请
    Immunoassay element 失效

    公开(公告)号：US20060024770A1

    公开(公告)日：2006-02-02

    申请号：US11230127

    申请日：2005-09-19

    申请人： Yoshiki Sakaino ,  Hitomi Ito ,  Toshihiro Mori ,  Osamu Seshimoto ,  Toshihisa Ito ,  Yoshikazu Amano

    发明人： Yoshiki Sakaino ,  Hitomi Ito ,  Toshihiro Mori ,  Osamu Seshimoto ,  Toshihisa Ito ,  Yoshikazu Amano

    IPC分类号： G01N33/537 ,  G01N33/53 ,  G01N33/543

    CPC分类号： G01N33/54386 ,  G01N2333/924 ,  Y10S435/966 ,  Y10S435/969 ,  Y10S435/97 ,  Y10S436/807 ,  Y10S436/81

    摘要： The immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity of an enzyme-labelled antigen or antibody caused by an immunological reaction. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. As the non-diffusible substrate, a substrate capable of reacting solely with the lebelling enzyme and incapable of reacting the fragmenting enzyme is utilized. When an endo-active glucosidase is used as the labelling enzyme, and an exo-active glucosidase is used the fragmenting enzyme in the reagent layer, the non-diffusible substrate of the substrate layer is preferred to be an endo type selectively reactive substrate, which means a substrate having a reactivity specific to endo-active glucosidase. Highly sensitive assay is realized with high accuracy and high reproducibility and good storage stability.

    Immunoassay element
    3.
    发明授权
    Immunoassay element 失效
    免疫测定元件

    公开(公告)号：US07820402B2

    公开(公告)日：2010-10-26

    申请号：US11230127

    申请日：2005-09-19

    申请人： Yoshiki Sakaino ,  Hitomi Ito ,  Toshihiro Mori ,  Osamu Seshimoto ,  Toshihisa Ito ,  Yoshikazu Amano

    发明人： Yoshiki Sakaino ,  Hitomi Ito ,  Toshihiro Mori ,  Osamu Seshimoto ,  Toshihisa Ito ,  Yoshikazu Amano

    IPC分类号： G01N33/53 ,  G01N33/543

    CPC分类号： G01N33/54386 ,  G01N2333/924 ,  Y10S435/966 ,  Y10S435/969 ,  Y10S435/97 ,  Y10S436/807 ,  Y10S436/81

    摘要： The immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity of an enzyme-labelled antigen or antibody caused by an immunological reaction. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. As the non-diffusible substrate, a substrate capable of reacting solely with the labelling enzyme and incapable of reacting the fragmenting enzyme is utilized. When an endo-active glucosidase is used as the labelling enzyme, and an exo-active glucosidase is used the fragmenting enzyme in the reagent layer, the non-diffusible substrate of the substrate layer is preferred to be an endo type selectively reactive substrate, which means a substrate having a reactivity specific to endo-active glucosidase. Highly sensitive assay is realized with high accuracy and high reproducibility and good storage stability.

    摘要翻译： 用于通过确定由免疫反应引起的酶标记的抗原或抗体的酶活性的变化来定量分析抗原的免疫测定元件。 免疫测定元件包括含有在标记酶存在下形成可扩散材料的不扩散基底的底物层和含有用于进一步将可扩散材料分解成较低分子量产物的碎裂酶的试剂层。 作为非扩散性底物，使用能够仅与标记酶反应并且不能使片段化酶反应的底物。 当使用内源活性葡糖苷酶作为标记酶，并且使用外源活性葡糖苷酶时，试剂层中的断裂酶，底物层的非扩散性基质优选为内型选择性反应性底物，其中 是指具有对内源性葡糖苷酶特异性的反应性的底物。 高精度，高重现性和良好的储存稳定性实现了高灵敏度测定。

    Immunoassay element
    4.
    发明申请
    Immunoassay element 审中-公开
    免疫测定元件

    公开(公告)号：US20050084953A1

    公开(公告)日：2005-04-21

    申请号：US10961459

    申请日：2004-10-07

    申请人： Yoshiki Sakaino ,  Hitomi Ito ,  Toshihiro Mori ,  Osamu Seshimoto ,  Toshihisa Ito ,  Yoshikazu Amano

    发明人： Yoshiki Sakaino ,  Hitomi Ito ,  Toshihiro Mori ,  Osamu Seshimoto ,  Toshihisa Ito ,  Yoshikazu Amano

    IPC分类号： G01N33/543 ,  G01N33/53

    CPC分类号： G01N33/54386 ,  G01N2333/924 ,  Y10S435/966 ,  Y10S435/969 ,  Y10S435/97 ,  Y10S436/807 ,  Y10S436/81

    摘要： The immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity of an enzyme-labelled antigen or antibody caused by an immunological reaction. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. As the non-diffusible substrate, a substrate capable of reacting solely with the lebelling enzyme and incapable of reacting the fragmenting enzyme is utilized. When an endo-active glucosidase is used as the labelling enzyme, and an exo-active glucosidase is used the fragmenting enzyme in the reagent layer, the non-diffusible substrate of the substrate layer is preferred to be an endo type selectively reactive substrate, which means a substrate having a reactivity specific to endo-active glucosidase. Highly sensitive assay is realized with high accuracy and high reproducibility and good storage stability.

    摘要翻译： 用于通过确定由免疫反应引起的酶标记的抗原或抗体的酶活性的变化来定量分析抗原的免疫测定元件。 免疫测定元件包括含有在标记酶存在下形成可扩散材料的不扩散基底的底物层和含有用于进一步将可扩散材料分解成较低分子量产物的碎裂酶的试剂层。 作为非扩散性底物，可以使用能够仅与前体酶反应并且不能使片段化酶反应的底物。 当使用内源活性葡糖苷酶作为标记酶，并且使用外源活性葡糖苷酶时，试剂层中的断裂酶，底物层的非扩散性基质优选为内型选择性反应性底物，其中 是指具有对内源性葡糖苷酶特异性的反应性的底物。 高精度，高重现性和良好的储存稳定性实现了高灵敏度测定。

    Ion selective electrode
    5.
    发明授权
    Ion selective electrode 有权
    离子选择电极

    公开(公告)号：US06767450B2

    公开(公告)日：2004-07-27

    申请号：US09823840

    申请日：2001-03-30

    申请人： Masaaki Terashima ,  Osamu Seshimoto ,  Yoshikazu Amano

    发明人： Masaaki Terashima ,  Osamu Seshimoto ,  Yoshikazu Amano

    IPC分类号： G01N27333

    CPC分类号： G01N27/333

    摘要： A composite ion selective electrode comprising a calcium ion selective electrode member and a hydrogen ion selective electrode member which is characterized in that a calcium ion selective membrane of the calcium ion selective electrode member has a thickness of 5 to 30 &mgr;m, and the hydrogen ion selective membrane of the hydrogen ion selective electrode member contains tri-n-dodecyl-amine and trisethylhexyl trimellitate. Each of the calcium ion selective electrode member and the hydrogen ion selective electrode member can be employed singly.

    摘要翻译： 1.一种复合离子选择电极，其包含钙离子选择电极部件和氢离子选择电极部件，其特征在于，所述钙离子选择电极部件的钙离子选择性膜的厚度为5〜30μm，氢离子选择性 氢离子选择电极构件的膜包含三正十二烷基胺和偏苯三酸三乙基己酯。 钙离子选择电极构件和氢离子选择电极构件中的每一个可以单独使用。

    Analytical element for measurements of enzyme activity
    7.
    发明授权
    Analytical element for measurements of enzyme activity 失效
    酶活性测定用分析元素

    公开(公告)号：US5508173A

    公开(公告)日：1996-04-16

    申请号：US373885

    申请日：1995-01-17

    申请人： Yoshikazu Amano ,  Kazuya Kawasaki ,  Harumi Katsuyama

    发明人： Yoshikazu Amano ,  Kazuya Kawasaki ,  Harumi Katsuyama

    IPC分类号： G01N31/22 ,  C12Q1/00 ,  G01N33/52 ,  C12Q1/28 ,  C12Q1/26 ,  C12Q1/52

    CPC分类号： G01N33/525 ,  G01N33/526

    摘要： A multilayer analytical element for measuring enzyme activity in a liquid sample such as a body fluid which is less affected by intrinsic pyruvic acid contained in the liquid sample is disclosed. The analytical element comprises a support, a reagent layer, an optionally provided other layer, and a porous spreading layer. The reagent layer and/or the optionally provided other layer contain pyruvic acid oxidase. The characteristic feature of the analytical element lies in that the spreading layer has a specific void volume in the range of 3-15 .mu.l/cm.sup.2.

    摘要翻译： 公开了一种用于测量液体样品中的酶活性的多层分析元件，所述液体样品例如体液中较少受到液体样品中所含的固有丙酮酸的影响。 分析元件包括载体，试剂层，任选提供的其它层和多孔扩散层。 试剂层和/或任选提供的其它层含有丙酮酸氧化酶。 分析元件的特征在于扩散层具有在3-15μml/ cm 2范围内的特定空隙体积。

    Method for correcting instrumental error of spectroscope of optical analyzer
    9.
    发明授权
    Method for correcting instrumental error of spectroscope of optical analyzer 失效
    光学分析仪分光仪器误差校正方法

    公开(公告)号：US5592290A

    公开(公告)日：1997-01-07

    申请号：US358486

    申请日：1994-11-14

    申请人： Fuminori Arai ,  Yoshikazu Amano ,  Kiyoshi Yamada

    发明人： Fuminori Arai ,  Yoshikazu Amano ,  Kiyoshi Yamada

    IPC分类号： G01N33/52 ,  G01N21/27 ,  G01N21/78 ,  G01N21/86 ,  G01N31/22 ,  G01J3/00

    CPC分类号： G01N21/8483 ,  G01N21/274

    摘要： A method for correcting an instrumental error due to wavelength error of a spectroscope of an optical analyzer for measuring an optical density reflected from or transmitted through a dry analysis element. One standard color plate having a standard optical density OD.sub.ST is measured by an optical analyzer to be corrected to obtain an measured value OD.sub.M. A measured optical density OD.sub.S of the element applied with a sample through the optical analyzer to be corrected is corrected by using a ratio of OD.sub.M /OD.sub.ST to obtain a corrected measured value OD.sub.C of the sample. The standard color plate to be used contains an indicator dye contained in the non-reacted dry analysis element or a dye having an absorption spectrum same as or similar to that of the indicator dye in the measurement wavelength range. Another correction method in consideration of a layer coefficient of a dry analysis element is also provided.

    摘要翻译： 一种用于校正由用于测量从干分析元件反射或透过干光分析元件的光密度的光学分析仪的分光镜引起的波长误差的仪器误差的方法。 通过光学分析器测量具有标准光密度ODST的一个标准色板以进行校正，以获得测量值ODM。 通过使用ODM / ODST的比例来校正通过待校正的光学分析器施加样品的元件的测量光密度ODS，以获得样品的校正测量值ODC。 所使用的标准色板包含未反应干燥分析元件中所含的指示剂染料或在测量波长范围内具有与指示剂染料相同或类似的吸收光谱的染料。 还提供了考虑干分析元件的层系数的另一校正方法。