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    FUSION PROTEIN CAPABLE OF BINDING VEGF-A AND TNF-ALPHA
    2.
    发明申请
    FUSION PROTEIN CAPABLE OF BINDING VEGF-A AND TNF-ALPHA 有权
    融合蛋白能够结合VEGF-A和TNF-α

    公开(公告)号：US20100150926A1

    公开(公告)日：2010-06-17

    申请号：US12636013

    申请日：2009-12-11

    申请人： Keehoon JUNG ,  Young Jun Koh ,  Gyun Min Lee ,  Sun Chang Kim ,  Gou Young Koh

    发明人： Keehoon JUNG ,  Young Jun Koh ,  Gyun Min Lee ,  Sun Chang Kim ,  Gou Young Koh

    IPC分类号： A61K39/395 ,  C07K16/00 ,  C07H21/04 ,  C12N15/63 ,  C12N5/10 ,  C12P21/06 ,  A61P19/02

    CPC分类号： C07K16/241 ,  C07K14/71 ,  C07K14/715 ,  C07K16/22 ,  C07K2317/31 ,  C07K2319/70

    摘要： The present application describes an isolated nucleic acid molecule encoding a polypeptide capable of synchronously binding VEGF polypeptide and TNF polypeptide comprising: (a) a nucleotide sequence encoding a TNFR2 component and VEGFR1 component operatively linked to (b) a nucleotide sequence encoding a multimerizing component, wherein the TNFR2 component consists essentially of a nucleotide sequence encoding the amino acid sequences of cystein rich domain 1, cystein rich domain 2, cystein rich domain 3, and cystein rich domain 4 of the extracellular domain of TNFR2, and wherein the VEGFR1 component consists essentially of a nucleotide sequence encoding the amino acid sequences of Ig-like domain 2 of the extracellular domain of VEGFR1.

    摘要翻译： 本申请描述了编码能够同时结合VEGF多肽和TNF多肽的多肽的分离的核酸分子，其包含：（a）编码TNFR2组分的核苷酸序列和可操作地连接到（b）编码多聚化成分的核苷酸序列的VEGFR1组分， 其中TNFR2组分基本上由编码TNFR2细胞外结构域的富含半胱氨酸域1，半胱氨酸富含结构域2，半胱氨酸富含结构域3和半胱氨酸富含结构域4的氨基酸序列的核苷酸序列组成，其中VEGFR1组分基本上组成 的编码VEGFR1细胞外结构域的Ig样结构域2的氨基酸序列的核苷酸序列。

    Fusion protein capable of binding VEGF-A and TNF-alpha
    3.
    发明授权
    Fusion protein capable of binding VEGF-A and TNF-alpha 有权
    能够结合VEGF-A和TNF-α的融合蛋白

    公开(公告)号：US08927232B2

    公开(公告)日：2015-01-06

    申请号：US12636013

    申请日：2009-12-11

    申请人： Keehoon Jung ,  Young Jun Koh ,  Gyun Min Lee ,  Sun Chang Kim ,  Gou Young Koh

    发明人： Keehoon Jung ,  Young Jun Koh ,  Gyun Min Lee ,  Sun Chang Kim ,  Gou Young Koh

    IPC分类号： A61K38/17 ,  C07K14/71 ,  C07K14/715 ,  C12N5/10 ,  C12N15/12

    CPC分类号： C07K16/241 ,  C07K14/71 ,  C07K14/715 ,  C07K16/22 ,  C07K2317/31 ,  C07K2319/70

    摘要： The present application describes an isolated nucleic acid molecule encoding a polypeptide capable of synchronously binding VEGF polypeptide and TNF polypeptide comprising: (a) a nucleotide sequence encoding a TNFR2 component and VEGFR1 component operatively linked to (b) a nucleotide sequence encoding a multimerizing component, wherein the TNFR2 component consists essentially of a nucleotide sequence encoding the amino acid sequences of cystein rich domain 1, cystein rich domain 2, cystein rich domain 3, and cystein rich domain 4 of the extracellular domain of TNFR2, and wherein the VEGFR1 component consists essentially of a nucleotide sequence encoding the amino acid sequences of Ig-like domain 2 of the extracellular domain of VEGFR1.

    摘要翻译： 本申请描述了编码能够同时结合VEGF多肽和TNF多肽的多肽的分离的核酸分子，其包含：（a）编码TNFR2组分的核苷酸序列和可操作地连接到（b）编码多聚化成分的核苷酸序列的VEGFR1组分， 其中TNFR2组分基本上由编码TNFR2细胞外结构域的富含半胱氨酸域1，半胱氨酸富含结构域2，半胱氨酸富含结构域3和半胱氨酸富含结构域4的氨基酸序列的核苷酸序列组成，其中VEGFR1组分基本上组成 的编码VEGFR1细胞外结构域的Ig样结构域2的氨基酸序列的核苷酸序列。

    Inhibition of apoptosis by the expression of antisense RNA of caspase-3
    5.
    发明授权
    Inhibition of apoptosis by the expression of antisense RNA of caspase-3 失效
    通过caspase-3的反义RNA的表达抑制细胞凋亡

    公开(公告)号：US06943022B2

    公开(公告)日：2005-09-13

    申请号：US10245813

    申请日：2002-09-17

    申请人： Gyun Min Lee ,  No Soo Kim

    发明人： Gyun Min Lee ,  No Soo Kim

    IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K48/00 ,  A61P43/00 ,  C12N15/113 ,  C12N15/57 ,  C12N5/00 ,  C07H21/04

    CPC分类号： C12N15/1137 ,  A61K38/00 ,  C12N2310/111 ,  C12Y304/22056

    摘要： The present invention relates to an antisense nucleotide of caspase-3, an expression vector encoding antisense RNA of caspase-3, and an inhibition method of cellular apoptosis in recombinant Chinese hamster ovary (CHO) cells using the same. More precisely, the present invention relates to an antisense nucleotide composed of a base sequence represented by Sequence ID No. 1, which suppresses the expression of caspase-3, an expression vector containing the above antisense nucleotide and expressing antisense RNA of caspase-3, and an inhibition method of apoptosis of recombinant cells by suppressing the expression of caspase-3 through introducing the above expression vector into the recombinant cells and then expressing antisense RNA of caspase-3. According to the present invention, apoptosis can be inhibited by suppressing the activation of caspase-3, which is related to the apoptosis, and the integrity of target protein produced in the recombinant cells can be enhanced.

    摘要翻译： 本发明涉及半胱天冬酶-3的反义核苷酸，编码半胱天冬酶-3的反义RNA的表达载体，以及使用其的重组中国仓鼠卵巢（CHO）细胞中的细胞凋亡的抑制方法。 更准确地说，本发明涉及由序列号1表示的碱基序列构成的反义核苷酸，其抑制半胱天冬酶-3的表达，含有上述反义核苷酸并表达半胱天冬酶-3的反义RNA的表达载体， 以及通过将上述表达载体导入重组细胞中，然后表达半胱天冬酶-3的反义RNA，通过抑制半胱天冬酶-3的表达来抑制重组细胞凋亡的抑制方法。 根据本发明，通过抑制与凋亡相关的半胱天冬酶-3的活化，可以抑制细胞凋亡，能够提高重组细胞中产生的靶蛋白的完整性。