公开(公告)号：US20120322116A1

公开(公告)日：2012-12-20

申请号：US13497894

申请日：2010-09-24

申请人： Keishi Sakaguchi ,  Takanori Matsuda ,  Takumi Kobayashi ,  Makoto Ito ,  Naoki Nagano ,  Masahiro Hayashi ,  Daisuke Honda ,  Yosuke Taoka ,  Yuji Okita ,  Hitoshi Izumida ,  Shinichi Sugimoto

发明人： Keishi Sakaguchi ,  Takanori Matsuda ,  Takumi Kobayashi ,  Makoto Ito ,  Naoki Nagano ,  Masahiro Hayashi ,  Daisuke Honda ,  Yosuke Taoka ,  Yuji Okita ,  Hitoshi Izumida ,  Shinichi Sugimoto

IPC分类号： C12P7/64 ,  C12N15/79

CPC分类号： C12P7/6409 ,  C11C3/00 ,  C12N9/0071 ,  C12N9/0083 ,  C12N15/79 ,  C12N15/8201 ,  C12N15/8247 ,  C12P7/6427

摘要： Disclosed is a transformation method whereby an ability to produce a useful substance of a stramenopile can be improved. The method for transforming a stramenopile comprises transferring a foreign gene into the stramenopile which is a microorganism belonging to the class Labyrinthula, more specifically, to a genus Labyrinthula, Altornia, Aplanochytrium, Schizochytrium, Aurantiochytrium, Thraustochytrium, Ulkenia, etc. Said foreign gene, which is a gene relating to tolerance against an antibiotic, a colorimetric protein and/or a fatty acid desaturase (Δ5 desaturase gene, Δ12 desaturase gene and/or ω3 desaturase gene), is transferred by using the electroporation or gene-gun technique.

摘要翻译： 公开了能够提高产生有机物的能力的转化方法。 用于转化stramenopile的方法包括将外源基因转移到作为属于Labyrinthula类的微生物的地幔中，更具体地是属于Labyrinthula，Altornia，Aplanochytrium，Schizochytrium，Aurantiochytrium，Thraustochytrium，Ulkenia等。所述外源基因， 其是与抗生素，比色蛋白质和/或脂肪酸去饱和酶（＆Dgr。5去饱和酶基因，＆Dgr; 12去饱和酶基因和/或ω3去饱和酶基因）的耐受相关的基因通过使用电穿孔或基因 - 枪技术。

公开(公告)号：US09150891B2

公开(公告)日：2015-10-06

申请号：US13497894

申请日：2010-09-24

申请人： Keishi Sakaguchi ,  Takanori Matsuda ,  Takumi Kobayashi ,  Makoto Ito ,  Naoki Nagano ,  Masahiro Hayashi ,  Daisuke Honda ,  Yosuke Taoka ,  Yuji Okita ,  Hitoshi Izumida ,  Shinichi Sugimoto

发明人： Keishi Sakaguchi ,  Takanori Matsuda ,  Takumi Kobayashi ,  Makoto Ito ,  Naoki Nagano ,  Masahiro Hayashi ,  Daisuke Honda ,  Yosuke Taoka ,  Yuji Okita ,  Hitoshi Izumida ,  Shinichi Sugimoto

IPC分类号： C12P7/64 ,  C12P21/06 ,  C12N1/00 ,  C12N1/10 ,  C12N15/00 ,  C11C3/00 ,  C12N9/02 ,  C12N15/79 ,  C12N15/82

CPC分类号： C12P7/6409 ,  C11C3/00 ,  C12N9/0071 ,  C12N9/0083 ,  C12N15/79 ,  C12N15/8201 ,  C12N15/8247 ,  C12P7/6427

摘要： Disclosed is a transformation method whereby an ability to produce a useful substance of a stramenopile can be improved. The method for transforming a stramenopile comprises transferring a foreign gene into the stramenopile which is a microorganism belonging to the class Labyrinthula, more specifically, to a genus Labyrinthula, Altornia, Aplanochytrium, Schizochytrium, Aurantiochytrium, Thraustochytrium, Ulkenia, etc. Said foreign gene, which is a gene relating to tolerance against an antibiotic, a colorimetric protein and/or a fatty acid desaturase (Δ5 desaturase gene, Δ12 desaturase gene and/or ω3 desaturase gene), is transferred by using the electroporation or gene-gun technique.

摘要翻译： 公开了能够提高产生有机物的能力的转化方法。 用于转化stramenopile的方法包括将外源基因转移到作为属于Labyrinthula类的微生物的地幔中，更具体地是属于Labyrinthula，Altornia，Aplanochytrium，Schizochytrium，Aurantiochytrium，Thraustochytrium，Ulkenia等。所述外源基因， 其是与抗生素，比色蛋白质和/或脂肪酸去饱和酶（＆Dgr。5去饱和酶基因，＆Dgr; 12去饱和酶基因和/或ω3去饱和酶基因）的耐受相关的基因通过使用电穿孔或基因 - 枪技术。

公开(公告)号：US20120100550A1

公开(公告)日：2012-04-26

申请号：US13254794

申请日：2010-03-04

申请人： Naoki Nagano ,  Masahiro Hayashi ,  Keishi Sakaguchi ,  Makoto Ito ,  Yosuke Taoka ,  Yuji Okita

发明人： Naoki Nagano ,  Masahiro Hayashi ,  Keishi Sakaguchi ,  Makoto Ito ,  Yosuke Taoka ,  Yuji Okita

IPC分类号： C12Q1/68

CPC分类号： C12Q1/689

摘要： Disclosed is a method for determining the fatty acid synthesis pathway of a microorganism. Specifically disclosed is a method for determining the fatty acid synthesis pathway of a microorganism, which is characterized by producing degenerate primers for a fatty acid synthesis-related enzyme gene and determining the presence or absence of the fatty acid synthesis-related gene in the genome gene extracted from the microorganism using the degenerate primers. The sequences for degenerate primers for C20-elongase gene, which is a fatty acid synthesis-related enzyme gene, are ATHGARTWYTKBRTITTYGTICA (SEQ ID NO:1) and TARTRISWRTACATIADIAMRTG (SEQ ID NO:2), and the sequences for degenerate primers for ?4-desaturase gene are GGNCAYCAYCMITAYACNAA (SEQ ID NO:3) and TCDATYTGRTGIBWIARNCC (SEQ ID NO:4). The microorganism is one belonging to the class Labyrinthulea. Also specifically disclosed is a PCR primer set for use in the determination method. The method is useful as a method for screening a microorganism capable of synthesizing a fatty acid.

摘要翻译： 公开了一种测定微生物的脂肪酸合成途径的方法。 具体公开的是测定微生物的脂肪酸合成途径的方法，其特征在于制备脂肪酸合成相关酶基因的简并引物，并测定基因组基因中脂肪酸合成相关基因的存在或不存在 使用简并引物从微生物中提取。 作为脂肪酸合成相关酶基因的C20延伸酶基因的简并引物的序列是ATHGARTWYTKBRTITTYGTICA（SEQ ID NO：1）和TARTRISWRTACATIADIAMRTG（SEQ ID NO：2），并且用于Δ4的简并引物的序列 - 脱氢酶基因是GGNCAYCAYCMITAYACNAA（SEQ ID NO：3）和TCDATYTGRTGIBWIARNCC（SEQ ID NO：4）。 该微生物属于Labyrinthulea类。 还具体公开了用于确定方法的PCR引物组。 该方法可用作筛选能够合成脂肪酸的微生物的方法。

公开(公告)号：US08349595B2

公开(公告)日：2013-01-08

申请号：US12524647

申请日：2008-01-25

申请人： Masahiro Hayashi ,  Sanae Nakajima ,  Naoki Nagano ,  Yosuke Taoka

发明人： Masahiro Hayashi ,  Sanae Nakajima ,  Naoki Nagano ,  Yosuke Taoka

IPC分类号： C12P7/64 ,  C12N1/12 ,  C07C57/03

CPC分类号： C12P7/6427 ,  C11C3/00 ,  C12N1/14 ,  C12P7/6472

摘要： [PROBLEMS] To provide a fat-and-oil in which the content of docosahexaenoic acid is increased.[MEANS FOR SOLVING PROBLEMS] A process for producing highly unsaturated fatty acids comprising culturing a stramenopile capable of producing highly unsaturated fatty acids in a culture medium containing an inhibitor for fatty acid desaturases; fats-and-oils in which the content of highly unsaturated fatty acids, particularly docosahexaenoic acid produced by the relevant method, is increased. A method for enhancing the productivity of highly unsaturated fatty acids in stramenopiles, comprising culturing a stramenopile in a culture medium containing an inhibitor for fatty acid desaturases; stramenopiles having the enhanced productivity of highly unsaturated fatty acids, generated by the relevant method. Particularly, the process for producing highly unsaturated fatty acids, the method for enhancing the productivity of highly unsaturated fatty acids, and the stramenopile having the enhanced productivity of highly unsaturated fatty acids, wherein the stramenopile is a microorganism classified into Labyrinthulea.

摘要翻译： [问题]提供其中二十二碳六烯酸含量增加的脂肪和油。 解决问题的手段一种高不饱和脂肪酸的制造方法，其特征在于，在含有脂肪酸去饱和酶抑制剂的培养基中培养能够生产高度不饱和脂肪酸的前列腺素; 其中通过相关方法生产的高度不饱和脂肪酸，特别是二十二碳六烯酸的含量增加的脂肪和油。 一种增强层状高分子量不饱和脂肪酸的生产率的方法，包括在含有脂肪酸去饱和酶抑制剂的培养基中培养前列腺素; 具有通过相关方法生成的高度不饱和脂肪酸的生产率提高的前列腺素。 特别是生产高不饱和脂肪酸的方法，提高高不饱和脂肪酸的生产率的方法以及具有提高的高度不饱和脂肪酸的生产率的前列腺素，其中所述地釉菌是分类为迷迭虫的微生物。

公开(公告)号：US20100099901A1

公开(公告)日：2010-04-22

申请号：US12524647

申请日：2008-01-25

申请人： Masahiro Hayashi ,  Sanae Nakajima ,  Naoki Nagano ,  Yosuke Taoka

发明人： Masahiro Hayashi ,  Sanae Nakajima ,  Naoki Nagano ,  Yosuke Taoka

IPC分类号： C07C57/03 ,  C12P7/64 ,  C12N1/12

CPC分类号： C12P7/6427 ,  C11C3/00 ,  C12N1/14 ,  C12P7/6472

摘要： [PROBLEMS] To provide a fat-and-oil in which the content of docosahexaenoic acid is increased.[MEANS FOR SOLVING PROBLEMS] A process for producing highly unsaturated fatty acids comprising culturing a stramenopile capable of producing highly unsaturated fatty acids in a culture medium containing an inhibitor for fatty acid desaturases; fats-and-oils in which the content of highly unsaturated fatty acids, particularly docosahexaenoic acid produced by the relevant method, is increased. A method for enhancing the productivity of highly unsaturated fatty acids in stramenopiles, comprising culturing a stramenopile in a culture medium containing an inhibitor for fatty acid desaturases; stramenopiles having the enhanced productivity of highly unsaturated fatty acids, generated by the relevant method. Particularly, the process for producing highly unsaturated fatty acids, the method for enhancing the productivity of highly unsaturated fatty acids, and the stramenopile having the enhanced productivity of highly unsaturated fatty acids, wherein the stramenopile is a microorganism classified into Labyrinthulea.

摘要翻译： [问题]提供其中二十二碳六烯酸含量增加的脂肪和油。 解决问题的手段一种高不饱和脂肪酸的制造方法，其特征在于，在含有脂肪酸去饱和酶抑制剂的培养基中培养能够生产高度不饱和脂肪酸的前列腺素; 其中通过相关方法生产的高度不饱和脂肪酸，特别是二十二碳六烯酸的含量增加的脂肪和油。 一种增强层状高分子量不饱和脂肪酸的生产率的方法，包括在含有脂肪酸去饱和酶抑制剂的培养基中培养前列腺素; 具有通过相关方法生成的高度不饱和脂肪酸的生产率提高的前列腺素。 特别是生产高不饱和脂肪酸的方法，提高高不饱和脂肪酸的生产率的方法以及具有提高的高度不饱和脂肪酸的生产率的前列腺素，其中所述地釉菌是分类为迷迭虫的微生物。