公开(公告)号：US20120322116A1

公开(公告)日：2012-12-20

申请号：US13497894

申请日：2010-09-24

申请人： Keishi Sakaguchi ,  Takanori Matsuda ,  Takumi Kobayashi ,  Makoto Ito ,  Naoki Nagano ,  Masahiro Hayashi ,  Daisuke Honda ,  Yosuke Taoka ,  Yuji Okita ,  Hitoshi Izumida ,  Shinichi Sugimoto

发明人： Keishi Sakaguchi ,  Takanori Matsuda ,  Takumi Kobayashi ,  Makoto Ito ,  Naoki Nagano ,  Masahiro Hayashi ,  Daisuke Honda ,  Yosuke Taoka ,  Yuji Okita ,  Hitoshi Izumida ,  Shinichi Sugimoto

IPC分类号： C12P7/64 ,  C12N15/79

CPC分类号： C12P7/6409 ,  C11C3/00 ,  C12N9/0071 ,  C12N9/0083 ,  C12N15/79 ,  C12N15/8201 ,  C12N15/8247 ,  C12P7/6427

摘要： Disclosed is a transformation method whereby an ability to produce a useful substance of a stramenopile can be improved. The method for transforming a stramenopile comprises transferring a foreign gene into the stramenopile which is a microorganism belonging to the class Labyrinthula, more specifically, to a genus Labyrinthula, Altornia, Aplanochytrium, Schizochytrium, Aurantiochytrium, Thraustochytrium, Ulkenia, etc. Said foreign gene, which is a gene relating to tolerance against an antibiotic, a colorimetric protein and/or a fatty acid desaturase (Δ5 desaturase gene, Δ12 desaturase gene and/or ω3 desaturase gene), is transferred by using the electroporation or gene-gun technique.

摘要翻译： 公开了能够提高产生有机物的能力的转化方法。 用于转化stramenopile的方法包括将外源基因转移到作为属于Labyrinthula类的微生物的地幔中，更具体地是属于Labyrinthula，Altornia，Aplanochytrium，Schizochytrium，Aurantiochytrium，Thraustochytrium，Ulkenia等。所述外源基因， 其是与抗生素，比色蛋白质和/或脂肪酸去饱和酶（＆Dgr。5去饱和酶基因，＆Dgr; 12去饱和酶基因和/或ω3去饱和酶基因）的耐受相关的基因通过使用电穿孔或基因 - 枪技术。

公开(公告)号：US09150891B2

公开(公告)日：2015-10-06

申请号：US13497894

申请日：2010-09-24

申请人： Keishi Sakaguchi ,  Takanori Matsuda ,  Takumi Kobayashi ,  Makoto Ito ,  Naoki Nagano ,  Masahiro Hayashi ,  Daisuke Honda ,  Yosuke Taoka ,  Yuji Okita ,  Hitoshi Izumida ,  Shinichi Sugimoto

发明人： Keishi Sakaguchi ,  Takanori Matsuda ,  Takumi Kobayashi ,  Makoto Ito ,  Naoki Nagano ,  Masahiro Hayashi ,  Daisuke Honda ,  Yosuke Taoka ,  Yuji Okita ,  Hitoshi Izumida ,  Shinichi Sugimoto

IPC分类号： C12P7/64 ,  C12P21/06 ,  C12N1/00 ,  C12N1/10 ,  C12N15/00 ,  C11C3/00 ,  C12N9/02 ,  C12N15/79 ,  C12N15/82

CPC分类号： C12P7/6409 ,  C11C3/00 ,  C12N9/0071 ,  C12N9/0083 ,  C12N15/79 ,  C12N15/8201 ,  C12N15/8247 ,  C12P7/6427

摘要： Disclosed is a transformation method whereby an ability to produce a useful substance of a stramenopile can be improved. The method for transforming a stramenopile comprises transferring a foreign gene into the stramenopile which is a microorganism belonging to the class Labyrinthula, more specifically, to a genus Labyrinthula, Altornia, Aplanochytrium, Schizochytrium, Aurantiochytrium, Thraustochytrium, Ulkenia, etc. Said foreign gene, which is a gene relating to tolerance against an antibiotic, a colorimetric protein and/or a fatty acid desaturase (Δ5 desaturase gene, Δ12 desaturase gene and/or ω3 desaturase gene), is transferred by using the electroporation or gene-gun technique.

摘要翻译： 公开了能够提高产生有机物的能力的转化方法。 用于转化stramenopile的方法包括将外源基因转移到作为属于Labyrinthula类的微生物的地幔中，更具体地是属于Labyrinthula，Altornia，Aplanochytrium，Schizochytrium，Aurantiochytrium，Thraustochytrium，Ulkenia等。所述外源基因， 其是与抗生素，比色蛋白质和/或脂肪酸去饱和酶（＆Dgr。5去饱和酶基因，＆Dgr; 12去饱和酶基因和/或ω3去饱和酶基因）的耐受相关的基因通过使用电穿孔或基因 - 枪技术。

公开(公告)号：US20130309772A1

公开(公告)日：2013-11-21

申请号：US13877225

申请日：2011-09-30

申请人： Keishi Sakaguchi ,  Rie Hamaguchi ,  Takanori Matsuda ,  Makoto Ito ,  Naoki Nagano ,  Masahiro Hayashi ,  Daisuke Honda ,  Yuji Okita ,  Shinichi Sugimoto

发明人： Keishi Sakaguchi ,  Rie Hamaguchi ,  Takanori Matsuda ,  Makoto Ito ,  Naoki Nagano ,  Masahiro Hayashi ,  Daisuke Honda ,  Yuji Okita ,  Shinichi Sugimoto

IPC分类号： C12N15/79 ,  C12N15/89

CPC分类号： C12N15/79 ,  C12N9/0071 ,  C12N9/0083 ,  C12N9/1029 ,  C12N15/113 ,  C12N15/52 ,  C12N15/895 ,  C12N2310/11 ,  C12N2310/14 ,  C12P7/6409 ,  C12P7/6427 ,  C12P7/6472 ,  C12Y114/19001 ,  C12Y114/19006 ,  C12Y203/01119

摘要： To provide a transformation method for producing a stramenopile organism having an improved unsaturated fatty acid production capability by disrupting a gene of the stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner. [Solution] A method for transforming a stramenopile organism, which comprises disrupting a gene of the stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner, and which is characterized in that the stramenopile organism is selected from Thraustochytrium aureum, Parietichytrium sarkarianum, Thraustochytrium roseum and Parietichytrium sp. and the gene to be disrupted or of which the expression is to be inhibited is a gene associated with the biosynthesis of a fatty acid.

摘要翻译： 提供通过以遗传工程方式破坏该层状生物体的基因或抑制该基因的表达来生产具有改善的不饱和脂肪酸生产能力的前烧石生物的转化方法。 [解决方案]一种转基因生物的方法，其包括以基因工程方式破坏所述地精生物体的基因或抑制所述基因的表达，其特征在于，所述前列腺生物体选自甲状腺破囊壶菌（Salisochytrium aureum），，鱼（Parietichytrium sarkarianum） ，玫瑰色胸，和Parietichytrium sp。 并且待破坏的基因或其表达被抑制的基因是与脂肪酸的生物合成相关的基因。

公开(公告)号：US09062315B2

公开(公告)日：2015-06-23

申请号：US13877225

申请日：2011-09-30

申请人： Keishi Sakaguchi ,  Rie Hamaguchi ,  Takanori Matsuda ,  Makoto Ito ,  Naoki Nagano ,  Masahiro Hayashi ,  Daisuke Honda ,  Yuji Okita ,  Shinichi Sugimoto

发明人： Keishi Sakaguchi ,  Rie Hamaguchi ,  Takanori Matsuda ,  Makoto Ito ,  Naoki Nagano ,  Masahiro Hayashi ,  Daisuke Honda ,  Yuji Okita ,  Shinichi Sugimoto

IPC分类号： C12N15/79 ,  C12N15/89 ,  C12N9/02 ,  C12N9/10 ,  C12P7/64 ,  C12N15/52

CPC分类号： C12N15/79 ,  C12N9/0071 ,  C12N9/0083 ,  C12N9/1029 ,  C12N15/113 ,  C12N15/52 ,  C12N15/895 ,  C12N2310/11 ,  C12N2310/14 ,  C12P7/6409 ,  C12P7/6427 ,  C12P7/6472 ,  C12Y114/19001 ,  C12Y114/19006 ,  C12Y203/01119

摘要： To provide a transformation method for producing a stramenopile organism having an improved unsaturated fatty acid production capability by disrupting a gene of the stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner. [Solution] A method for transforming a stramenopile organism, which comprises disrupting a gene of the stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner, and which is characterized in that the stramenopile organism is selected from Thraustochytrium aureum, Parietichytrium sarkarianum, Thraustochytrium roseum and Parietichytrium sp. and the gene to be disrupted or of which the expression is to be inhibited is a gene associated with the biosynthesis of a fatty acid.

摘要翻译： 提供通过以遗传工程方式破坏该层状生物体的基因或抑制该基因的表达来生产具有改善的不饱和脂肪酸生产能力的前烧石生物的转化方法。 [解决方案]一种转基因生物的方法，其包括以基因工程方式破坏所述地精生物体的基因或抑制所述基因的表达，其特征在于，所述前列腺生物体选自甲状腺破囊壶菌（Salisochytrium aureum），，鱼（Parietichytrium sarkarianum） ，玫瑰色胸，和Parietichytrium sp。 并且待破坏的基因或其表达被抑制的基因是与脂肪酸的生物合成相关的基因。

公开(公告)号：US20120100550A1

公开(公告)日：2012-04-26

申请号：US13254794

申请日：2010-03-04

申请人： Naoki Nagano ,  Masahiro Hayashi ,  Keishi Sakaguchi ,  Makoto Ito ,  Yosuke Taoka ,  Yuji Okita

发明人： Naoki Nagano ,  Masahiro Hayashi ,  Keishi Sakaguchi ,  Makoto Ito ,  Yosuke Taoka ,  Yuji Okita

IPC分类号： C12Q1/68

CPC分类号： C12Q1/689

摘要： Disclosed is a method for determining the fatty acid synthesis pathway of a microorganism. Specifically disclosed is a method for determining the fatty acid synthesis pathway of a microorganism, which is characterized by producing degenerate primers for a fatty acid synthesis-related enzyme gene and determining the presence or absence of the fatty acid synthesis-related gene in the genome gene extracted from the microorganism using the degenerate primers. The sequences for degenerate primers for C20-elongase gene, which is a fatty acid synthesis-related enzyme gene, are ATHGARTWYTKBRTITTYGTICA (SEQ ID NO:1) and TARTRISWRTACATIADIAMRTG (SEQ ID NO:2), and the sequences for degenerate primers for ?4-desaturase gene are GGNCAYCAYCMITAYACNAA (SEQ ID NO:3) and TCDATYTGRTGIBWIARNCC (SEQ ID NO:4). The microorganism is one belonging to the class Labyrinthulea. Also specifically disclosed is a PCR primer set for use in the determination method. The method is useful as a method for screening a microorganism capable of synthesizing a fatty acid.

摘要翻译： 公开了一种测定微生物的脂肪酸合成途径的方法。 具体公开的是测定微生物的脂肪酸合成途径的方法，其特征在于制备脂肪酸合成相关酶基因的简并引物，并测定基因组基因中脂肪酸合成相关基因的存在或不存在 使用简并引物从微生物中提取。 作为脂肪酸合成相关酶基因的C20延伸酶基因的简并引物的序列是ATHGARTWYTKBRTITTYGTICA（SEQ ID NO：1）和TARTRISWRTACATIADIAMRTG（SEQ ID NO：2），并且用于Δ4的简并引物的序列 - 脱氢酶基因是GGNCAYCAYCMITAYACNAA（SEQ ID NO：3）和TCDATYTGRTGIBWIARNCC（SEQ ID NO：4）。 该微生物属于Labyrinthulea类。 还具体公开了用于确定方法的PCR引物组。 该方法可用作筛选能够合成脂肪酸的微生物的方法。

公开(公告)号：US08349595B2

公开(公告)日：2013-01-08

申请号：US12524647

申请日：2008-01-25

申请人： Masahiro Hayashi ,  Sanae Nakajima ,  Naoki Nagano ,  Yosuke Taoka

发明人： Masahiro Hayashi ,  Sanae Nakajima ,  Naoki Nagano ,  Yosuke Taoka

IPC分类号： C12P7/64 ,  C12N1/12 ,  C07C57/03

CPC分类号： C12P7/6427 ,  C11C3/00 ,  C12N1/14 ,  C12P7/6472

摘要： [PROBLEMS] To provide a fat-and-oil in which the content of docosahexaenoic acid is increased.[MEANS FOR SOLVING PROBLEMS] A process for producing highly unsaturated fatty acids comprising culturing a stramenopile capable of producing highly unsaturated fatty acids in a culture medium containing an inhibitor for fatty acid desaturases; fats-and-oils in which the content of highly unsaturated fatty acids, particularly docosahexaenoic acid produced by the relevant method, is increased. A method for enhancing the productivity of highly unsaturated fatty acids in stramenopiles, comprising culturing a stramenopile in a culture medium containing an inhibitor for fatty acid desaturases; stramenopiles having the enhanced productivity of highly unsaturated fatty acids, generated by the relevant method. Particularly, the process for producing highly unsaturated fatty acids, the method for enhancing the productivity of highly unsaturated fatty acids, and the stramenopile having the enhanced productivity of highly unsaturated fatty acids, wherein the stramenopile is a microorganism classified into Labyrinthulea.

摘要翻译： [问题]提供其中二十二碳六烯酸含量增加的脂肪和油。 解决问题的手段一种高不饱和脂肪酸的制造方法，其特征在于，在含有脂肪酸去饱和酶抑制剂的培养基中培养能够生产高度不饱和脂肪酸的前列腺素; 其中通过相关方法生产的高度不饱和脂肪酸，特别是二十二碳六烯酸的含量增加的脂肪和油。 一种增强层状高分子量不饱和脂肪酸的生产率的方法，包括在含有脂肪酸去饱和酶抑制剂的培养基中培养前列腺素; 具有通过相关方法生成的高度不饱和脂肪酸的生产率提高的前列腺素。 特别是生产高不饱和脂肪酸的方法，提高高不饱和脂肪酸的生产率的方法以及具有提高的高度不饱和脂肪酸的生产率的前列腺素，其中所述地釉菌是分类为迷迭虫的微生物。

公开(公告)号：US20100099901A1

公开(公告)日：2010-04-22

申请号：US12524647

申请日：2008-01-25

申请人： Masahiro Hayashi ,  Sanae Nakajima ,  Naoki Nagano ,  Yosuke Taoka

发明人： Masahiro Hayashi ,  Sanae Nakajima ,  Naoki Nagano ,  Yosuke Taoka

IPC分类号： C07C57/03 ,  C12P7/64 ,  C12N1/12

CPC分类号： C12P7/6427 ,  C11C3/00 ,  C12N1/14 ,  C12P7/6472

摘要： [PROBLEMS] To provide a fat-and-oil in which the content of docosahexaenoic acid is increased.[MEANS FOR SOLVING PROBLEMS] A process for producing highly unsaturated fatty acids comprising culturing a stramenopile capable of producing highly unsaturated fatty acids in a culture medium containing an inhibitor for fatty acid desaturases; fats-and-oils in which the content of highly unsaturated fatty acids, particularly docosahexaenoic acid produced by the relevant method, is increased. A method for enhancing the productivity of highly unsaturated fatty acids in stramenopiles, comprising culturing a stramenopile in a culture medium containing an inhibitor for fatty acid desaturases; stramenopiles having the enhanced productivity of highly unsaturated fatty acids, generated by the relevant method. Particularly, the process for producing highly unsaturated fatty acids, the method for enhancing the productivity of highly unsaturated fatty acids, and the stramenopile having the enhanced productivity of highly unsaturated fatty acids, wherein the stramenopile is a microorganism classified into Labyrinthulea.

摘要翻译： [问题]提供其中二十二碳六烯酸含量增加的脂肪和油。 解决问题的手段一种高不饱和脂肪酸的制造方法，其特征在于，在含有脂肪酸去饱和酶抑制剂的培养基中培养能够生产高度不饱和脂肪酸的前列腺素; 其中通过相关方法生产的高度不饱和脂肪酸，特别是二十二碳六烯酸的含量增加的脂肪和油。 一种增强层状高分子量不饱和脂肪酸的生产率的方法，包括在含有脂肪酸去饱和酶抑制剂的培养基中培养前列腺素; 具有通过相关方法生成的高度不饱和脂肪酸的生产率提高的前列腺素。 特别是生产高不饱和脂肪酸的方法，提高高不饱和脂肪酸的生产率的方法以及具有提高的高度不饱和脂肪酸的生产率的前列腺素，其中所述地釉菌是分类为迷迭虫的微生物。

公开(公告)号：US08528717B2

公开(公告)日：2013-09-10

申请号：US12198532

申请日：2008-08-26

申请人： Yukio Ando ,  Masahiro Hayashi

发明人： Yukio Ando ,  Masahiro Hayashi

IPC分类号： B65G15/64

CPC分类号： B41J3/4078 ,  B41F17/005 ,  B41J3/28 ,  B41J11/06

摘要： A printing apparatus including a pallet configured to receive a print substrate, a conveyer system configured to convey the pallet, and a plurality of work mechanisms arranged along a pallet conveying direction of the conveyer system. The work mechanisms are configured to conduct processing operations to the print substrate. The conveyer system includes an upper conveyer mechanism configured to sequentially convey the pallet to respective working positions of the work mechanisms, and a lower conveyer mechanism arranged below and along the upper conveyer mechanism. The lower conveyer mechanism is configured to convey the pallet in a direction opposite to the pallet conveying direction of the upper conveyer mechanism so that the conveyer system is structured to have an upper stage and a lower stage.

摘要翻译： 一种打印装置，包括构造成接收打印基板的托盘，构造成输送托盘的输送器系统以及沿着输送器系统的托盘输送方向布置的多个工作机构。 工作机构被配置为对打印基板进行处理操作。 输送机系统包括上部输送机构，其构造成将托盘顺序地输送到工作机构的相应工作位置，以及下部输送机构，其布置在上部输送机构的下方并沿着该输送机构。 下输送机构构造成沿着与上输送机构的托盘输送方向相反的方向输送托盘，使得输送机系统被构造成具有上段和下段。

公开(公告)号：US20130206287A1

公开(公告)日：2013-08-15

申请号：US13816905

申请日：2011-08-15

申请人： Jun Sato ,  Shinya Imano ,  Mototsugu Osaki ,  Shigeki Ueta ,  Kiyohito Ishida ,  Toshihiro Omori ,  Hiroaki Nishida ,  Masahiro Hayashi ,  Tomoki Shiota

发明人： Jun Sato ,  Shinya Imano ,  Mototsugu Osaki ,  Shigeki Ueta ,  Kiyohito Ishida ,  Toshihiro Omori ,  Hiroaki Nishida ,  Masahiro Hayashi ,  Tomoki Shiota

IPC分类号： C22F1/10 ,  C22C30/00 ,  C22C19/05

CPC分类号： C22F1/10 ,  C22C19/055 ,  C22C19/056 ,  C22C19/057 ,  C22C30/00 ,  F01D5/28 ,  F05C2201/0463 ,  F05D2300/17 ,  Y02T50/671

摘要： A Co-based alloy containing not less than 0.001 mass % and less than 0.100 mass % of C, not less than 9.0 mass % and less than 20.0 mass % of Cr, not less than 2.0 mass % and less than 5.0 mass % of Al, not less than 13.0 mass % and less than 20.0 mass % of W, and not less than 39.0 mass % and less than 55.0 mass % of Ni, with the remainder being made up by Co and unavoidable impurities, wherein the contents of Mo, Nb, Ti and Ta which are included in the unavoidable impurities are as follows: Mo

摘要翻译： 含有不小于0.001质量％且小于0.100质量％的C，不小于9.0质量％且小于20.0质量％的Cr的Co基合金，不小于2.0质量％且小于5.0质量％的Al ，不小于13.0质量％且小于20.0质量％的W，不小于39.0质量％且小于55.0质量％的Ni，其余由Co和不可避免的杂质构成，其中Mo， 包含在不可避免的杂质中的Nb，Ti和Ta如下：Mo <0.010质量％，Nb <0.010质量％，Ti <0.010质量％，Ta <0.010质量％。

公开(公告)号：US08416822B2

公开(公告)日：2013-04-09

申请号：US13127531

申请日：2009-11-12

申请人： Hiroyoshi Shouji ,  Shunichi Sato ,  Toshihiro Ishii ,  Kengo Makita ,  Masahiro Hayashi ,  Toshihide Sasaki ,  Akihiro Itoh

发明人： Hiroyoshi Shouji ,  Shunichi Sato ,  Toshihiro Ishii ,  Kengo Makita ,  Masahiro Hayashi ,  Toshihide Sasaki ,  Akihiro Itoh

IPC分类号： H01S5/00

CPC分类号： H01S5/18361 ,  H01S5/0202 ,  H01S5/18311 ,  H01S5/3202 ,  H01S5/423 ,  H01S2301/173 ,  H01S2301/176

摘要： A manufacturing method for manufacturing a surface-emitting laser device includes the steps of forming a laminated body in which a lower reflecting mirror, a resonator structure including an active layer, and an upper reflecting layer having a selective oxidized layer are laminated on a substrate; etching the laminated body to form a mesa structure having the selective oxidized layer exposed at side surfaces thereof; selectively oxidizing the selective oxidized layer from the side surfaces of the mesa structure to form a constriction structure in which a current passing region is surrounded by an oxide; forming a separating groove at a position away from the mesa structure; passivating an outermost front surface of at least a part of the laminated body exposed when the separating groove is formed; and coating a passivated part with a dielectric body.

摘要翻译： 一种制造表面发射激光器件的制造方法包括以下步骤：在基板上层压下反射镜，包括有源层的谐振器结构和具有选择性氧化层的上反射层的层叠体; 蚀刻层叠体以形成在其侧表面露出的选择性氧化层的台面结构; 从台面结构的侧面选择性地氧化选择氧化层，形成电流通过区被氧化物包围的收缩结构; 在远离台面结构的位置处形成分隔槽; 钝化形成分离槽时暴露的层叠体的至少一部分的最外表面; 并用绝缘体涂覆钝化部分。