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    Liposome immunoassay method and kit therefor
    1.
    发明授权
    Liposome immunoassay method and kit therefor 失效
    LIPOSOME免疫方法和套件

    公开(公告)号：US5173406A

    公开(公告)日：1992-12-22

    申请号：US339824

    申请日：1989-01-05

    申请人： Kenji Hosoda ,  Hideaki Suzuki ,  Tatsuji Yasuda

    发明人： Kenji Hosoda ,  Hideaki Suzuki ,  Tatsuji Yasuda

    IPC分类号： G01N33/543 ,  G01N33/544 ,  G01N33/577 ,  G01N33/58

    CPC分类号： G01N33/5432 ,  G01N33/586 ,  Y10S435/966 ,  Y10S435/968 ,  Y10S435/975 ,  Y10S436/80 ,  Y10S436/805 ,  Y10S436/821 ,  Y10S436/829

    摘要： A liposome immunoassay comprising the steps of reacting an analyte antigen, a liposome bearing antibody comprising a first antibody to the analyte antigen and a liposome encapsulating a marker therein linked to the antibody, and a second antibody to the analyte antigen to form an antigen-antibody complex, releasing the marker from the liposome in an amount depending on an amount of the analyte antigen in the presence of a complement, and measuring the released marker to determine the analyte antigen, characterized in using a third antibody capable of binding directly or indirectly to the second antibody and having an ability to activate the complement; andA kit for liposome immunoassay comprising at least one of; (1) an liposome bearing antibody comprising a first antibody to an analyte antigen and liposome encapsulating a marker therein linked to the antibody; (2) a second antibody to the analyte antigen; (3) a third antibody capable of binding directly or indirectly to the second antibody and having an ability to activate a complement; and (4) the complement.

    Quantitation of human pulmonary surfactant and reagent kit to be used therefor
    2.
    发明授权
    Quantitation of human pulmonary surfactant and reagent kit to be used therefor 失效
    人类使用的人肺表面活性剂和试剂盒的定量

    公开(公告)号：US5156950A

    公开(公告)日：1992-10-20

    申请号：US638026

    申请日：1991-01-07

    申请人： Toyoaki Akino ,  Yoshio Kuroki ,  Kenji Hosoda ,  Hideaki Suzuki

    发明人： Toyoaki Akino ,  Yoshio Kuroki ,  Kenji Hosoda ,  Hideaki Suzuki

    IPC分类号： C07K16/18 ,  G01N33/68

    CPC分类号： G01N33/6884 ,  C07K16/18 ,  Y10S435/962 ,  Y10S435/975

    摘要： A method of quantitation of human pulmonary surfactant characterized by determining the quantity of human pulmonary surfactant in the test sample according to the immunological method by use of the primary monoclonal antibodies which recognize the human pulmonary surfactant apoproteins and the labeled secondary monoclonal antibodies which recognize said apoproteins but bind to a part of the antigen which is different from the one to which the primary monoclonal antibodies bind, and a reagent kit to be used for the quantitation.

    摘要翻译： 一种定量人肺表面活性剂的方法，其特征在于根据免疫学方法通过识别人肺表面活性蛋白的首要单克隆抗体和识别所述脱辅基蛋白的标记的次级单克隆抗体来测定测试样品中人肺表面活性剂的量 但是结合到与一级单克隆抗体结合的抗原的不同部分的抗原和用于定量的试剂盒。

    Method of determining human acid glutathione S-transferase, reagent, therefor, kit therefor, method of diagnosing cancer in digestive organs, and monoclonal antibody for use therein
    4.
    发明授权
    Method of determining human acid glutathione S-transferase, reagent, therefor, kit therefor, method of diagnosing cancer in digestive organs, and monoclonal antibody for use therein 失效
    确定人酸谷胱甘肽S-转移酶的方法，其试剂，用于其的试剂盒，消化器官中的癌症诊断方法和用于其中的单克隆抗体

    公开(公告)号：US5427917A

    公开(公告)日：1995-06-27

    申请号：US169963

    申请日：1993-12-20

    申请人： Kenji Hosoda ,  Hitomi Honda ,  Takaharu Kubota ,  Hideaki Suzuki

    发明人： Kenji Hosoda ,  Hitomi Honda ,  Takaharu Kubota ,  Hideaki Suzuki

    IPC分类号： C07K16/30 ,  C07K16/40 ,  G01N33/53

    CPC分类号： G01N33/5306 ,  C07K16/3046 ,  C07K16/40

    摘要： A method of immunologically determining human acid glutathione S-transferase in a human assay sample, which comprises bringing the assay sample into contact with a first antibody bound to an insoluble solid carrier and a labelled second antibody, either the first or second antibody being a polyclonal antibody capable of recognizing human acid glutathione S-transferase or an equivalent fragment of the polyclonal antibody, and the other antibody being a monoclonal antibody capable of specifically recognizing human acid glutathione S-transferase or an equivalent fragment of the monoclonal antibody, a method of diagnosing cancer in a human digestive organ by using the above method, a reagent therefor, a kit therefor and a monoclonal antibody for use therein.

    摘要翻译： 一种在人测定样品中免疫测定人酸谷胱甘肽S-转移酶的方法，其包括使测定样品与结合于不溶性固体载体和标记的第二抗体的第一抗体接触，所述第一或第二抗体是多克隆 能够识别人酸谷胱甘肽S-转移酶或多克隆抗体的等同片段的抗体，另一抗体是能够特异性识别人酸谷胱甘肽S-转移酶或单克隆抗体的等同片段的单克隆抗体，诊断方法 通过使用上述方法的人类消化器官中的癌症，其试剂，用于其的试剂盒和用于其中的单克隆抗体。

    Immunoassay and reagent kit used therefor
    5.
    发明授权
    Immunoassay and reagent kit used therefor 失效
    用于此的免疫测定和试剂盒

    公开(公告)号：US5366861A

    公开(公告)日：1994-11-22

    申请号：US26110

    申请日：1993-03-01

    申请人： Kenji Hosoda ,  Takaharu Kubota ,  Hitomi Honda ,  Hideaki Suzuki

    发明人： Kenji Hosoda ,  Takaharu Kubota ,  Hitomi Honda ,  Hideaki Suzuki

    IPC分类号： G01N33/53 ,  G01N33/543 ,  G01N33/86 ,  C12Q1/00 ,  C12Q1/48 ,  G01N33/536

    CPC分类号： G01N33/5306 ,  G01N33/54393 ,  G01N33/86 ,  G01N2333/745 ,  G01N2333/81 ,  G01N2333/96461 ,  Y10S435/975

    摘要： An immunoassay method performed in solution wherein a protein having an average molecular weight of 16,000 to 50,000 and an isoelectric point of 1.0 to 5.0 or a mixture containing the same is permitted to exist as the antigen-antibody reaction controller in the immunoreaction solution, and the final concentration of the antigen-antibody controller in the immunoreaction solution is controlled to 0.02 to 0.9% by weight, and a reagent kit to be used for an immunoassay containing the above antigen-antibody reaction controller at a final concentration of 0.02 to 0.9% by weight in the immunoreaction solution, as a part of the constituents thereof.

    摘要翻译： 在溶液中进行的免疫测定方法，其中允许平均分子量为16,000至50,000，等电点为1.0至5.0的蛋白质或含有该蛋白质的混合物作为免疫反应溶液中的抗原 - 抗体反应控制剂存在， 将免疫反应溶液中的抗原 - 抗体控制剂的终浓度控制在0.02〜0.9重量％，将试剂盒用于含有上述抗原 - 抗体反应控制剂的终浓度为0.02〜0.9％的免疫测定 免疫反应溶液中的重量，作为其成分的一部分。

    Method of immunological assaying of human osteocalcin, reagent and kit therefor, antibody to human osteocalcin, hybridoma producing said antibody, and method of producing it
    8.
    发明授权
    Method of immunological assaying of human osteocalcin, reagent and kit therefor, antibody to human osteocalcin, hybridoma producing said antibody, and method of producing it 失效
    人骨钙蛋白的免疫学测定方法，其试剂及试剂盒，人骨钙蛋白抗体，产生所述抗体的杂交瘤及其生产方法

    公开(公告)号：US5506111A

    公开(公告)日：1996-04-09

    申请号：US316364

    申请日：1994-10-03

    申请人： Kenji Hosoda ,  Hitomi Honda ,  Takaharu Kubota ,  Yasuhiko Masuho

    发明人： Kenji Hosoda ,  Hitomi Honda ,  Takaharu Kubota ,  Yasuhiko Masuho

    IPC分类号： C07K14/78 ,  C07K16/18 ,  G01N33/68 ,  G01N33/53

    CPC分类号： C07K14/78 ,  C07K16/18 ,  G01N33/6887 ,  G01N2800/108 ,  Y10S435/975

    摘要： A method of immunologically assaying intact human osteocalcin in a human assay sample is provided by using an antibody having an epitope in a region of an amino acid sequence 1 to 20 on the N-terminal side of human osteocalcin and an antibody having an epitope in a region of an amino acid sequence 36 to 49 on the C-terminal side of human osteocalcin. A reagent and a kit therefor are provided. Furthermore, a method of immunologically assaying the total amount of human intact osteocalcin in a human assay sample, and a reagent and a kit therefor are also provided. A monoclonal antibody and a polyclonal antibody are used for the assay. A process for producing these antibodies, and utilization of these antibodies is described.

    摘要翻译： 通过使用具有在人骨钙蛋白的N末端侧上具有1至20个氨基酸的区域中的表位的抗体和在其中具有表位的抗体来提供在人测定样品中免疫测定完整人骨钙蛋白的方法 在人骨钙蛋白的C末端侧的氨基酸序列36〜49的区域。 提供了一种试剂及其试剂盒。 此外，还提供了在人测定样品中免疫测定人完整骨钙素总量的方法，以及试剂及其试剂盒。 单克隆抗体和多克隆抗体用于测定。 描述了制备这些抗体的方法和这些抗体的利用。

    Method of immunological assaying human osteocalcin, reagent and kit therefor, antibody to human osteocalcin, hybridoma producing said antibody, and method of producing it
    9.
    发明授权
    Method of immunological assaying human osteocalcin, reagent and kit therefor, antibody to human osteocalcin, hybridoma producing said antibody, and method of producing it 失效
    免疫学测定方法人骨钙蛋白，其试剂和试剂盒，抗人骨钙素抗体，产生所述抗体的杂交瘤及其生产方法

    公开(公告)号：US5681707A

    公开(公告)日：1997-10-28

    申请号：US465348

    申请日：1995-06-05

    申请人： Kenji Hosoda ,  Hitomi Honda ,  Takaharu Kubota ,  Yasuhiko Masuho

    发明人： Kenji Hosoda ,  Hitomi Honda ,  Takaharu Kubota ,  Yasuhiko Masuho

    IPC分类号： C07K14/78 ,  C07K16/18 ,  G01N33/68 ,  G01N33/535

    CPC分类号： C07K14/78 ,  C07K16/18 ,  G01N33/6887 ,  G01N2800/108 ,  Y10S435/975

    摘要： A method of immunologically assaying intact human osteocalcin in a human assay sample is provided by using an antibody having an epitope in a region of an amino acid sequence 1 to 20 on the N-terminal side of human osteocalcinand an antibody having an epitope in a region of an amino acid sequence 36 to 49 on the C-terminal side of human osteocalcin. Furthermore, a method of immunologically assaying the total amount of human intact osteocalcin in a human assay sample is provided. A reagent and a kit therefor are also described, as well as a monclonal antibody and a polyclonal antibody used for the assay a process for producing these antibodies; and a process for use of these antibodies.

    摘要翻译： 通过使用具有在人骨钙蛋白的N末端侧上具有1至20个氨基酸的区域中的表位的抗体和在区域​​中具有表位的抗体来提供在人测定样品中免疫测定完整人骨钙素的方法 的人骨钙蛋白C末端侧的氨基酸序列36〜49。 此外，提供了在人测定样品中免疫测定人完整骨钙素总量的方法。 还描述了一种试剂及其试剂盒，以及用于测定生产这些抗体的方法的单克隆抗体和多克隆抗体; 以及使用这些抗体的方法。