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520 条记录 (耗时 0.039 秒)

    Method for separating components in liquid specimen and apparatus used in said method
    1.
    发明授权
    Method for separating components in liquid specimen and apparatus used in said method 失效
    用于分离液体试样中的组分的方法和用于所述方法的装置

    公开(公告)号：US6149818A

    公开(公告)日：2000-11-21

    申请号：US386149

    申请日：1999-08-31

    申请人： Kenji Nakamura ,  Shinji Satomura ,  Masayoshi Hayashi ,  Hideyoshi Arashima ,  Tetsushi Okuyama ,  Nobuhiro Hanafusa ,  Koji Tanimizu

    发明人： Kenji Nakamura ,  Shinji Satomura ,  Masayoshi Hayashi ,  Hideyoshi Arashima ,  Tetsushi Okuyama ,  Nobuhiro Hanafusa ,  Koji Tanimizu

    IPC分类号： B01D15/08 ,  G01N30/16 ,  G01N30/24 ,  G01N30/40 ,  G01N30/88

    CPC分类号： G01N30/40 ,  B01D15/1807 ,  B01D15/14 ,  G01N2030/402 ,  G01N2030/407 ,  G01N30/16 ,  G01N30/24 ,  G01N30/88

    摘要： The object of the present invention is to provide a method for separating components in a liquid specimen and an apparatus used in said method, by which it is possible to avoid carryover even when the specimen injector is not washed using washing solution and also to avoid the increase of inner pressure in column of the separation device or ineffective separating operation even when many liquid specimens are separated by many times using the same separation device. In the present invention, the liquid specimen is introduced into the separation device from a direction opposite to eluting direction of the separation device which retains an adsorbent, and by separating and eluting the specimen from eluting outlet together with the eluant, trace quantity of insoluble substances and the specimen remained on the specimen introducing passage can be removed by elution procedure. As a result, it is possible to avoid carryover, to prevent the increase of inner pressure in the column of the separation device and to prevent ineffective separating operation caused by clogging of the column.

    摘要翻译： 本发明的目的是提供一种用于分离液体试样中的组分和所述方法中使用的装置的方法，即使当使用洗涤溶液不洗涤试样注射器时也可以避免携带，并且避免 即使使用相同的分离装置多次分离多个液体样品，分离装置的塔内部压力增加或分离操作无效。 在本发明中，将液体试样从与保留吸附剂的分离装置的洗脱方向相反的方向引入分离装置，通过从洗脱液与洗脱液一起分离和洗脱试样，追踪微量的不溶物 残留在样品导入通道上的样品可以通过洗脱程序除去。 结果，可以避免残留，以防止分离装置的塔中的内压增加，并且防止由于柱的堵塞而导致的无效的分离操作。

    Process for separating and measuring glycoprotein
    3.
    发明授权
    Process for separating and measuring glycoprotein 失效
    糖蛋白分离和测定方法

    公开(公告)号：US5591589A

    公开(公告)日：1997-01-07

    申请号：US340299

    申请日：1994-11-14

    申请人： Hideo Katoh ,  Kenji Nakamura ,  Shinji Satomura

    发明人： Hideo Katoh ,  Kenji Nakamura ,  Shinji Satomura

    IPC分类号： G01N33/574 ,  G01N33/68 ,  G01N33/53

    CPC分类号： G01N33/6803 ,  G01N33/57469 ,  G01N33/57476 ,  Y10S435/973 ,  Y10S436/827

    摘要： The degree of a structure change of the sugar chain of a glycoprotein caused by a disease can be measured with high precision rapidly by separating and measuring two or more forms of glycoproteins which are different in sugar chain structure but have substantially the same protein structure, by using a combination of a lectin capable of recognizing the specific sugar chain structure of at least one of these glycoprotein analytes to be measured, and a first antibody which has a property of binding to all the glycoprotein analytes but is kept from binding to glycoprotein analyte(s) having the lectin attached thereto; and separating and measuring glycoprotein analyte(s) having the first antibody attached thereto and glycoprotein analyte(s) having no first antibody attached thereto, by utilizing differences in properties between the former glycoprotein analyte(s) and the latter glycoprotein analyte(s).

    摘要翻译： 由疾病引起的糖蛋白的糖链的结构变化程度可以通过分离和测定两种或多种形式的糖链结构不同但具有基本相同的蛋白质结构的糖蛋白，通过快速测定，由 使用能够识别待测量的这些糖蛋白分析物中的至少一种的特定糖链结构的凝集素的组合和具有结合所有糖蛋白分析物但不与糖蛋白分析物结合的性质的第一抗体（ s）具有凝集素附着; 并且通过利用前一种糖蛋白分析物和后一种糖蛋白分析物之间的特性差异来分离和测量具有附着于其上的第一抗体的糖蛋白分析物和与其无关的第一抗体的糖蛋白分析物。

    Process for separating and measuring trace components
    4.
    发明授权
    Process for separating and measuring trace components 失效
    分离和测量痕量组分的方法

    公开(公告)号：US5780247A

    公开(公告)日：1998-07-14

    申请号：US488009

    申请日：1995-06-07

    申请人： Shinji Satomura ,  Kenji Nakamura ,  Shuji Matuura

    发明人： Shinji Satomura ,  Kenji Nakamura ,  Shuji Matuura

    IPC分类号： C12Q1/40 ,  C12Q1/48 ,  G01N33/543 ,  G01N33/76 ,  G01N33/574

    CPC分类号： G01N33/54306 ,  C12Q1/40 ,  C12Q1/48 ,  G01N33/76 ,  Y10S435/971 ,  Y10S435/973 ,  Y10S436/824 ,  Y10S436/827

    摘要： Two or more analytes having the same action, or having different actions in spite of their similar structures, or two or more analytes having the same action and the same detectable chemical characteristics, in samples derived from living bodies, etc., can be measured rapidly and easily by forming one or more complexes with one or more affinity substances, separating the complexes by using high pressure liquid chromatography, followed by measurement of the amount of an affinity substance or one of the analytes.

    摘要翻译： 具有相同作用的两种或更多种分析物，或者具有相似结构的不同作用，或者来自活体等的样品中具有相同作用和相同可检测化学特性的两种或更多种分析物可以快速测量 并且通过与一种或多种亲和物质形成一种或多种配合物，通过使用高压液相色谱法分离复合物，然后测量亲和物质或分析物之一，容易地。

    Electrophoresis
    5.
    发明授权
    Electrophoresis 有权
    电泳

    公开(公告)号：US07842175B2

    公开(公告)日：2010-11-30

    申请号：US10472753

    申请日：2002-04-03

    申请人： Tomohisa Kawabata ,  Kenji Nakamura ,  Shinji Satomura

    发明人： Tomohisa Kawabata ,  Kenji Nakamura ,  Shinji Satomura

    IPC分类号： G01N27/447

    CPC分类号： G01N33/532 ,  C12Q1/68 ,  G01N27/44726 ,  C12Q2565/125 ,  C12Q2563/179

    摘要： The invention relates to a method for separating a target for measurement utilizing electrophoresis, particularly capillary electrophoresis efficiently in high sensitivity and in a short period of time. It also relates to a method for measuring said target separated by said method for separation. The invention provides a method for separating a target for measurement and a method for measuring said target separated by said method for separation, characterized by using a substance to which is bound a nucleic acid chain labeled by a marker and which has an affinity for said target for measurement.

    摘要翻译： 本发明涉及一种利用电泳，特别是高灵敏度和短时间内有效毛细管电泳分离测定靶的方法。 本发明还涉及一种用于分离由所述分离方法分离的所述靶的测量方法。 本发明提供了一种用于分离测量目标的方法和由所述分离方法分离的用于测量所述靶的方法，其特征在于使用结合由标记物标记的核酸链的物质，所述核酸链对所述靶标具有亲和力 用于测量。

    Process for separating complex
    10.
    发明授权
    Process for separating complex 失效
    分离复合物的方法

    公开(公告)号：US5571729A

    公开(公告)日：1996-11-05

    申请号：US354264

    申请日：1994-12-12

    申请人： Kenji Nakamura ,  Taizo Hara ,  Hideo Katoh ,  Shinji Satomura

    发明人： Kenji Nakamura ,  Taizo Hara ,  Hideo Katoh ,  Shinji Satomura

    IPC分类号： G01N33/538 ,  G01N33/573

    CPC分类号： G01N33/538 ,  Y10S436/824

    摘要： This invention is intended to provide a process by which a complex formed by the interaction between one or more analytes to be measured and affinity substance can be more effectively separated from substances existing together therewith which tend to affect the detection of the complex, for example, free affinity substance, by using high pressure liquid chromatography (HPLC); and a process for measuring a trace component by utilizing said separating process.This invention is characterized in that a substance which has been modified with a substance capable of changing properties of the complex (a separation-improving substance) and has affinity for the complex is attached to the complex. Because of this characteristic, the invention is effective in that the position of elution of said complex in the HPLC can be freely controlled. Furthermore, it is markedly effective in that the process for measuring a trace component by utilizing the separating process of this invention makes it possible to carry out the measurement with higher precision as compared with conventional measuring processes using HPLC.

    摘要翻译： 本发明旨在提供一种方法，通过该方法可以将待测量的一种或多种待分析物与亲和物质之间的相互作用形成的复合物与其中存在的物质更有效地分离，这些物质倾向于影响复合物的检测，例如， 游离亲和性物质，通过使用高压液相色谱（HPLC）; 以及通过利用所述分离过程来测量痕量组分的方法。 本发明的特征在于，将具有能够改变复合物（分离改善物质）性质并对复合物具有亲和性的物质进行了改性的物质附着于该络合物。 由于该特征，本发明的效果在于可以自由地控制HPLC中所述络合物的洗脱位置。 此外，通过利用本发明的分离方法测量痕量组分的方法与使用HPLC的常规测量方法相比，可以更高精度地进行测量。